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Comments (4)

ziyewang avatar ziyewang commented on July 30, 2024

Hi,

Could it be that the entire path is too long, causing this issue?
"/scratch/project_2007362/sandro/HeP_samples/2_assembly/HeP-1057_1_month_metabinner/s2_output/metabinner_res/ensemble_res/greedy_cont_weight_3_mincomp_50.0_maxcont_15.0_bins/ensemble_3logtrans/addrefined 2and3comps/greedy_cont_weight_3_mincomp_50.0_maxcont_15.0_bins_res.tsv"

Are there any fasta files in this folder? "/scratch/project_2007362/sandro/HeP_samples/2_assembly/HeP-1057_1_month_metabinner/s2_output/metabinner_res/intermediate_result/partial_seed_kmeans_bacar_marker_seed_length_weight_3quarter_X_t_logtrans_result.tsv_bins_post_process_mincomp_70_mincont_50_bins"

Best,
Ziye

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Sanrrone avatar Sanrrone commented on July 30, 2024

hi,
fastas are there

ls /scratch/project_2007362/sandro/HeP_samples/2_assembly/HeP-1057_1_month_metabinner/s2_output/metabinner_res/intermediate_result/partial_seed_kmeans_bacar_marker_seed_length_weight_3quarter_X_t_logtrans_result.tsv_bins_post_process_mincomp_70_mincont_50_bins
0.fa                     1484_reclustered_164.fa  1671.fa  2830.fa
1193.fa                  1484_reclustered_176.fa  2530.fa  2856.fa
1484_reclustered_0.fa    1484_reclustered_51.fa   2554.fa  3018.fa
1484_reclustered_133.fa  1484_reclustered_67.fa   2732.fa  3704.fa

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ziyewang avatar ziyewang commented on July 30, 2024

Hi,

The issue might be due to missing necessary files (/scratch/project_2007362/sandro/HeP_samples/2_assembly/HeP-1057_1_month_metabinner/s2_output/metabinner_res/ensemble_res/greedy_cont_weight_3_mincomp_50.0_maxcont_15.0_bins/ensemble_3logtrans/...) required for generating the final bins. Unfortunately, MetaBinner did not provide the essential error logs.

You can find the script responsible for generating the file "/scratch/project_2007362/sandro/HeP_samples/2_assembly/HeP-1057_1_month_metabinner/s2_output/metabinner_res/ensemble_res/greedy_cont_weight_3_mincomp_50.0_maxcont_15.0_bins/ensemble_3logtrans/addrefined2and3comps_bins_dir.tsv" at https://github.com/ziyewang/MetaBinner/blob/master/run_metabinner.sh.

If there are no privacy concerns regarding the data, please feel free to send the MetaBinner output to my email ([email protected]), and I will check it.

Best,
Ziye

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Sanrrone avatar Sanrrone commented on July 30, 2024

Hi,
just closing the comment due to the sample is not suitable for HQ bins.

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