Something went wrong with running split_hhbins.py. about metabinner HOT 3 CLOSED

Hi, Georgios-Filis

Thanks for using MetaBinner. Could you please provide additional information about the contigs, such as the number of contigs? Do the contigs primarily consist of bacterial or archaeal sequences?

Could you please provide the "post_result.log" and "result.log" located in the result path "/user_path/Binning_Results/Bins/metabinner_res/"? Thanks very much.

Best wishes,
Ziye

from metabinner.

Dear Ziye Wang,
Thank you for your immediate response. The file with the contigs contains 1993 contigs. After filtering for contigs of length higher than 1000, 14 contigs remained. The (compressed) input FASTQ files have a size equal to 62 MBs and contain reads with lengths in the range of 25-36 nucleotides. The contigs probably primarily contain bacterial sequences.
I just run MetaBinner successfully based on another dataset of FASTQ files, of a few GBs in size, which contain reads with lengths in the range of 25-151 nucleotides which had formed 59408 contigs from which 16017 were above 1000 nucleotides in length.

Therefore, could the error occur in the first case due to the low number of contigs or/and the small lengths of the reads?

I have attached the two log files related to the dataset of FASTQ and contig files that lead to the error.
result.log
post_result.log

Sincerely,
Giorgos

from metabinner.

Hi, Giorgos

It seems that the contig count is low (14), and no single-copy marker genes (SCG) can be identified within these contigs.

Best,
Ziye

from metabinner.