How important is the base sequence format? about flashlfq HOT 4 CLOSED

So nice to hear from you. It's been a while. Rob should be into the office in a couple hours. I'm sure he'll let you know. I hope you and your lab are doing well.

from flashlfq.

Thanks! :) it's been super-hectic recently, but we're getting there... btw - really liked the Neofusion paper. super impressive!

David.

from flashlfq.

The "Full Sequence" can be whatever you want, but the "Base Sequence" must only be amino acids in the actual peptide sequence. So in this case you'd need:

Full Sequence: K.LVNEVTEFAK[+162.053]TC[+57.021]VADESAENC[+57.021]DK.S
Base Sequence: LVNEVTEFAKTCVADESAENCDK

In Excel, you can use the function:
=LEFT(RIGHT(A1,LEN(A1)-2),LEN(A1)-4)

to trim off the trailing amino acids before/after the periods. This will get you to:
LVNEVTEFAK[+162.053]TC[+57.021]VADESAENC[+57.021]DK

It's harder to remove the mass-differences via a formula in Excel, but you can do find+replace for [*] to an empty value.

Hope that helps! Happy to hear from you again.

from flashlfq.

Thanks!

from flashlfq.