crashed - invalid parametrization for the distribution about flashlfq HOT 6 CLOSED

can you try running quant with 2 files using the same parameters? If you get the same error, that will help us troubleshoot the problem.

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it runs to completion without errors when i use only 2 files out of the 30

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that's good and bad. suggests a bad file, which is harder to spot in a large set. good in the sense that the program seems to be working fine. Let me share this error w/ a colleague before I send you off on another errand

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After a lot of trial and error i've identified the offending files. Though I don't know what's wrong with them... I am using the '-calib.mzml' files that are in the Task2 folder of my metamorpheus run. That metamorpheus ran to completion without problems so the only errors come up with flashlfq. Could you please help me find what the problem is? I still want to use the data...happy to send along some files to your email to help with debugging

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Originally, I was using a custom database in fasta format containing proteins from Gencode, some novel ORFs, and some fungal proteins.
I used the same raw files and ran a new metamorpheus run searching only Uniprot human proteins + contaminants. When I run flashlfq with the same parameters as before on this output it runs to completion with all 30 files. I think the error has something to do with my search database...

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if you can provide the big database, I can see if it can be read successfully.

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