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View Code? Open in Web Editor NEWde Bruijn Workflow using Integral information of Short pair End reads
de Bruijn Workflow using Integral information of Short pair End reads
Hi,
Do you have some statistics regarding the running time of the assembler with how many CPU associated ?
Hello,
running Bwise returns this error
/home/alessandro/BWISE/Bwise.py -x reads.fa -t 2 -o test
*** This is BWISE - High order De Bruijn graph assembler ***
Binaries are in: /home/alessandro/BWISE/bin
The command line was: /home/alessandro/BWISE/Bwise.py -x reads.fa -t 2 -o test
Results will be stored in: /home/alessandro/Documents/PhD/Bwise_test/test
[14:48:48 18/09/2017] --> Starting Read Correction with Bloocoo...
Correction step 1
/home/alessandro/BWISE/bin/Bloocoo32 -file /home/alessandro/Documents/PhD/Bwise_test/reads.fa -slow -kmer-size 31 -nbits-bloom 24 -out reads_corrected1.fa -nb-cores 2
ln -fs /home/alessandro/Documents/PhD/Bwise_test/test/reads_corrected1.fa /home/alessandro/Documents/PhD/Bwise_test/test/reads_corrected.fa
[15:42:37 18/09/2017] --> Correction Done
Correction took: 0:53:49
[15:42:37 18/09/2017] --> Starting Graph construction and Super Reads generation...
Unexpected error during graph construction: <class 'FileNotFoundError'>
[FATAL ERROR]
Try `Bwise --help` for more information
zsh: exit 1 /home/alessandro/BWISE/Bwise.py -x reads.fa -t 2 -o test
I installed Bwise today, 18th of September 2017, so it's the very last version.
Any idea? the input file is in correct fasta format.
BWISE seems to be working fine as long as k<220, then fails
Here is the output:
Reads Correction
Graph construction 0
Read mapping on the graph 0
Graph construction 1
Read mapping on the graph 1
Graph construction 2
Read mapping on the graph 2
Graph construction 3
Read mapping on the graph 3
Graph construction 4
Read mapping on the graph 4
Graph construction 5
Read mapping on the graph 5
sh: line 1: 56060 Killed /cluster/scratch4/ucbtlot/BWISEbin/bgreat -k 220 -x reads_corrected1.fa -u reads_corrected2.fa -g dbg5.fa -t 10 -c -m 0 -e 1 >> logs/logBgreat 2>> logs/logBgreat
Graph construction 6
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 6
Graph construction 7
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 7
Graph construction 8
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 8
Graph construction 9
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 9
Graph construction 10
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 10
Graph construction 11
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 11
Graph construction 12
h5dump error: unable to get link info from "histogram/histogram"
Read mapping on the graph 12
Hi, I run BWISE (commit cac2d0) with minimal command bwise -x Sm2.trim.norm.180.all.fastq -t 32
and it crushed.
Std out:
2
Reads Correction
Graph construction 0
Read mapping on the graph 0
Graph construction 1
Read mapping on the graph 1
Graph construction 2
Read mapping on the graph 2
Graph construction 3
Read mapping on the graph 3
Graph construction 4
Read mapping on the graph 4
SuperReads Compactions
The end
Error log:
mkdir: cannot create directory `.': File exists
sh: line 1: 74736 Segmentation fault (core dumped) /scratch/beegfs/monthly/kjaron/src/BWISE/bin/numbersFilter paths 2 2 > cleanedPaths 2>> logs/logBgreat
SR load super reads
SR add reverse complements 0
sorting 0
remove inclusions 0
Traceback (most recent call last):
File "/scratch/beegfs/monthly/kjaron/src/BWISE/bin/K2000.py", line 232, in <module>
main()
File "/scratch/beegfs/monthly/kjaron/src/BWISE/bin/K2000.py", line 204, in main
SR=remove_strict_inclusions(SR)
File "/scratch/beegfs/monthly/kjaron/src/BWISE/bin/K2000.py", line 72, in remove_strict_inclusions
sys.stderr.write("Removing inclusions, "+str(checked)+" checked. Size SR "+str(len(SR))+" %.2f"%(100*checked/len(SR))+"%\n")
ZeroDivisionError: division by zero
Print GFA Nodes
100.00% -- 0 error(s)
Print GFA Edges
100.00%
Hi, I was able to compile BWISE but can't run the tests all the way through. It fails at this step:
#Graph 3: Construction...
#Current date & time Tue Jul 17 16:25:12 2018
/home/bsiranos/software/BWISE/bin/bcalm -max-memory 10000 -in /data/bsiranos/software/BWISE/data/testFolder/contigs_k101.fa -kmer-size 102 -abundance-min 1 -out /data/bsiranos/software/BWISE/data/testFolder/out -nb-cores 4
[ERROR] There was a problem generating "/data/bsiranos/software/BWISE/data/testFolder/out.unitigs.fa".
[FATAL ERROR] One or several files could not be generated.
To find out why, try `Bwise --help` and/or check the logs files of the various steps of the pipeline (logs/logBloocoo, logs/logBcalm, logs/logTips, logs/logBgreat, logs/logK2000).
IT WORKS !
N50: 1
L50: 1
N80: 1
L80: 2
N90: 1
L90: 2
Here's the tail end of testFolder/logs/logBcalm. Something related to hdf5?
debug: not deleting glue files
Finding links between unitigs 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 0 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (0kmers/0extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 1 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (0kmers/0extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 2 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (0kmers/0extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 3 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (0kmers/0extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 4 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (1kmers/1extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 5 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (1kmers/1extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 6 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (0kmers/0extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 1 pass 7 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
step 2 (0kmers/0extremities) 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
gathering links from disk 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
Done finding links between unitigs 16:25:10 memory [current, maxRSS]: [ 173, 173] MB
BCALM 2, git commit e9ba83c
setting storage type to hdf5
HDF5-DIAG: Error detected in HDF5 (1.8.18) thread 0:
#000: /home/bsiranos/software/BWISE/src/bcalm/gatb-core/gatb-core/thirdparty/hdf5/src/H5A.c line 407 in H5Aopen(): unable to load attribute info from object header for attribute: 'version'
major: Attribute
minor: Unable to initialize object
#001: /home/bsiranos/software/BWISE/src/bcalm/gatb-core/gatb-core/thirdparty/hdf5/src/H5Oattribute.c line 537 in H5O_attr_open_by_name(): can't locate attribute: 'version'
major: Attribute
minor: Object not found
HDF5-DIAG: Error detected in HDF5 (1.8.18) thread 0:
#000: /home/bsiranos/software/BWISE/src/bcalm/gatb-core/gatb-core/thirdparty/hdf5/src/H5A.c line 677 in H5Aget_space(): not an attribute
major: Invalid arguments to routine
minor: Inappropriate type
HDF5-DIAG: Error detected in HDF5 (1.8.18) thread 0:
#000: /home/bsiranos/software/BWISE/src/bcalm/gatb-core/gatb-core/thirdparty/hdf5/src/H5S.c line 883 in H5Sget_simple_extent_dims(): not a dataspace
major: Invalid arguments to routine
minor: Inappropriate type
HDF5-DIAG: Error detected in HDF5 (1.8.18) thread 0:
#000: /home/bsiranos/software/BWISE/src/bcalm/gatb-core/gatb-core/thirdparty/hdf5/src/H5A.c line 634 in H5Aread(): not an attribute
major: Invalid arguments to routine
minor: Inappropriate type
EXCEPTION: Unable to open bank '/data/bsiranos/software/BWISE/data/testFolder/contigs_k101.fa' (if it is a list of files, perhaps some of the files inside don't exist)
urbe@urbo214b[BWISE] python3 Bwise.py -x /media/urbe/MyDDrive/OriginalReads/ANature/ANature_interleave.fastq -o testANatureBWISE
*** This is BWISE - High order De Bruijn graph assembler ***
Binaries are in: /home/urbe/Tools/BWISE/bin
The command line was: Bwise.py -x /media/urbe/MyDDrive/OriginalReads/ANature/ANature_interleave.fastq -o testANatureBWISE
[Warning] testANatureBWISE directory already exists, BWISE will use it.
Results will be stored in: /home/urbe/Tools/BWISE/testANatureBWISE
[18:51:01 01/08/2017] --> Starting Read Correction with Bloocoo...
Correction step 1
/home/urbe/Tools/BWISE/bin/Bloocoo32 -file /media/urbe/MyDDrive/OriginalReads/ANature/ANature_interleave.fastq -slow -kmer-size 31 -nbits-bloom 24 -out reads_corrected1.fa -nb-cores 0
Correction step 2
/home/urbe/Tools/BWISE/bin/Bloocoo64 -file /home/urbe/Tools/BWISE/testANatureBWISE/reads_corrected1.fa -slow -kmer-size 63 -nbits-bloom 24 -out reads_corrected2.fa -nb-cores 0
ln -fs /home/urbe/Tools/BWISE/testANatureBWISE/reads_corrected2.fa /home/urbe/Tools/BWISE/testANatureBWISE/reads_corrected.fa
[23:37:27 01/08/2017] --> Correction Done
Correction took: 4:46:26
[23:37:27 01/08/2017] --> Starting Graph construction and Super Reads generation...
#Graph 1: Construction...
/home/urbe/Tools/BWISE/bin/bcalm -max-memory 10000 -in /home/urbe/Tools/BWISE/testANatureBWISE/bankBcalm.txt -kmer-size 31 -abundance-min 2 -out /home/urbe/Tools/BWISE/testANatureBWISE/out -nb-cores 20
#Graph cleaning...
/home/urbe/Tools/BWISE/bin/btrim out.unitigs.fa 31 60 20 8 5
[ERROR] There was a problem writing "/home/urbe/Tools/BWISE/testANatureBWISE/tipped_out.unitigs.fa".
[FATAL ERROR] One or more files could not be written.
Try `Bwise --help` for more information
This should allow us to obtain a better level of continuity when assembling haploid genome (or diploid genomes with a very low level of heterozygosity).
In the "stable" branch, we should specify the exact version for each dependant (to prevent that dependency updates break the pipeline); whereas the "dev" branch should use the latest version of each dependency.
GPL licenses mandate that a copy of the license be distributed with source and binaries. We need that file for Bioconda to comply with the license restrictions.
Hi,
I have run the assembly and apparently it works without error (see log below).
But I would like to know what is the name of the final file (the contigs) ?
`
./Bwise.py -x Sm2.trim.180.pair12.fastq -k 300 -t 30 -o out
Binaries are in: /scratch/local/monthly/ptranvan/BWISE/bin
The command line was: ./Bwise.py -x Sm2.trim.180.pair12.fastq -k 300 -t 30 -o out
Results will be stored in: /scratch/local/monthly/ptranvan/BWISE/out
[08:49:30 20/04/2017] --> Starting Read Correction with Bloocoo...
Correction step 1
/scratch/local/monthly/ptranvan/BWISE/bin/Bloocoo32 -file /scratch/local/monthly/ptranvan/BWISE/Sm2.trim.180.pair12.fastq -slow -kmer
-size 31 -nbits-bloom 24 -out reads_corrected1.fa -nb-cores 30
Correction step 2
/scratch/local/monthly/ptranvan/BWISE/bin/Bloocoo64 -file /scratch/local/monthly/ptranvan/BWISE/out/reads_corrected1.fa -slow -kmer-s
ize 63 -nbits-bloom 24 -out reads_corrected2.fa -nb-cores 30
Correction step 3
/scratch/local/monthly/ptranvan/BWISE/bin/Bloocoo128 -file /scratch/local/monthly/ptranvan/BWISE/out/reads_corrected2.fa -slow -kmer-
size 95 -nbits-bloom 24 -out reads_corrected3.fa -nb-cores 30
Correction step 4
/scratch/local/monthly/ptranvan/BWISE/bin/Bloocoo128 -file /scratch/local/monthly/ptranvan/BWISE/out/reads_corrected3.fa -slow -kmer-size 127 -nbits-bloom 24 -out reads_corrected4.fa -nb-cores 30
ln -fs /scratch/local/monthly/ptranvan/BWISE/out/reads_corrected4.fa /scratch/local/monthly/ptranvan/BWISE/out/reads_corrected.fa
[13:35:31 20/04/2017] --> Correction Done
Correction took: 4:46:01
[13:35:31 20/04/2017] --> Starting Graph construction and Super Reads generation...
#Graph 1: Construction...
/scratch/local/monthly/ptranvan/BWISE/bin/bcalm -in /scratch/local/monthly/ptranvan/BWISE/out/bankBcalm.txt -kmer-size 51 -abundance-min 2 -out /scratch/local/monthly/ptranvan/BWISE/out/out -nb-cores 30
#Cleaning...
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL out.unitigs.fa 50 49
/scratch/local/monthly/ptranvan/BWISE/bin/tipCleaner out_out.unitigs.fa.fa 50 101
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL tiped.fa 50 49
mv out_tiped.fa.fa dbg51.fa
#Read mapping with BGREAT...
/scratch/local/monthly/ptranvan/BWISE/bin/bgreat -M -k 51 -i 10 -x reads_corrected.fa -g dbg51.fa -t 30 -a 63 -m 0 -e 100
/scratch/local/monthly/ptranvan/BWISE/bin/numbersFilter paths 2 cleanedPaths_51 2 dbg51.fa 51
/scratch/local/monthly/ptranvan/BWISE/bin/run_K2000.sh cleanedPaths_51 dbg51.fa 51 compacted_unitigs_k51.gfa compacted_unitigs_k51.fa
#Graph 2: Construction...
/scratch/local/monthly/ptranvan/BWISE/bin/bcalm -in /scratch/local/monthly/ptranvan/BWISE/out/compacted_unitigs_k51.fa -kmer-size 101 -abundance-min 1 -out /scratch/local/monthly/ptranvan/BWISE/out/out -nb-cores 30
#Cleaning...
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL out.unitigs.fa 100 99
/scratch/local/monthly/ptranvan/BWISE/bin/tipCleaner out_out.unitigs.fa.fa 100 151
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL tiped.fa 100 99
mv out_tiped.fa.fa dbg101.fa
#Read mapping with BGREAT...
/scratch/local/monthly/ptranvan/BWISE/bin/bgreat -M -k 101 -i 10 -x reads_corrected.fa -g dbg101.fa -t 30 -a 63 -m 0 -e 100
/scratch/local/monthly/ptranvan/BWISE/bin/numbersFilter paths 2 cleanedPaths_101 2 dbg101.fa 101
/scratch/local/monthly/ptranvan/BWISE/bin/run_K2000.sh cleanedPaths_101 dbg101.fa 101 compacted_unitigs_k101.gfa compacted_unitigs_k101.fa
#Graph 3: Construction...
/scratch/local/monthly/ptranvan/BWISE/bin/bcalm -in /scratch/local/monthly/ptranvan/BWISE/out/compacted_unitigs_k101.fa -kmer-size 151 -abundance-min 1 -out /scratch/local/monthly/ptranvan/BWISE/out/out -nb-cores 30
#Cleaning...
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL out.unitigs.fa 150 149
/scratch/local/monthly/ptranvan/BWISE/bin/tipCleaner out_out.unitigs.fa.fa 150 201
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL tiped.fa 150 149
mv out_tiped.fa.fa dbg151.fa
#Read mapping with BGREAT...
/scratch/local/monthly/ptranvan/BWISE/bin/bgreat -M -k 151 -i 10 -x reads_corrected.fa -g dbg151.fa -t 30 -a 63 -m 0 -e 100
/scratch/local/monthly/ptranvan/BWISE/bin/numbersFilter paths 2 cleanedPaths_151 2 dbg151.fa 151
/scratch/local/monthly/ptranvan/BWISE/bin/run_K2000.sh cleanedPaths_151 dbg151.fa 151 compacted_unitigs_k151.gfa compacted_unitigs_k151.fa
#Graph 4: Construction...
/scratch/local/monthly/ptranvan/BWISE/bin/bcalm -in /scratch/local/monthly/ptranvan/BWISE/out/compacted_unitigs_k151.fa -kmer-size 201 -abundance-min 1 -out /scratch/local/monthly/ptranvan/BWISE/out/out -nb-cores 30
#Cleaning...
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL out.unitigs.fa 200 199
/scratch/local/monthly/ptranvan/BWISE/bin/tipCleaner out_out.unitigs.fa.fa 200 251
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL tiped.fa 200 199
mv out_tiped.fa.fa dbg201.fa
#Read mapping with BGREAT...
/scratch/local/monthly/ptranvan/BWISE/bin/bgreat -M -k 201 -i 10 -x reads_corrected.fa -g dbg201.fa -t 30 -a 63 -m 0 -e 100
/scratch/local/monthly/ptranvan/BWISE/bin/numbersFilter paths 2 cleanedPaths_201 2 dbg201.fa 201
/scratch/local/monthly/ptranvan/BWISE/bin/run_K2000.sh cleanedPaths_201 dbg201.fa 201 compacted_unitigs_k201.gfa compacted_unitigs_k201.fa
#Graph 5: Construction...
/scratch/local/monthly/ptranvan/BWISE/bin/bcalm -in /scratch/local/monthly/ptranvan/BWISE/out/compacted_unitigs_k201.fa -kmer-size 251 -abundance-min 1 -out /scratch/local/monthly/ptranvan/BWISE/out/out -nb-cores 30
#Cleaning...
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL out.unitigs.fa 250 249
/scratch/local/monthly/ptranvan/BWISE/bin/tipCleaner out_out.unitigs.fa.fa 250 301
/scratch/local/monthly/ptranvan/BWISE/bin/kMILL tiped.fa 250 249
mv out_tiped.fa.fa dbg251.fa
#Read mapping with BGREAT...
/scratch/local/monthly/ptranvan/BWISE/bin/bgreat -M -k 251 -i 10 -x reads_corrected.fa -g dbg251.fa -t 30 -a 63 -m 0 -e 100
/scratch/local/monthly/ptranvan/BWISE/bin/numbersFilter paths 2 cleanedPaths_251 2 dbg251.fa 251
/scratch/local/monthly/ptranvan/BWISE/bin/run_K2000.sh cleanedPaths_251 dbg251.fa 251 compacted_unitigs_k251.gfa compacted_unitigs_k251.fa
[08:34:52 25/04/2017] --> Done!
Graph Construction took: 114:59:20
The end !
BWISE assembly took: 119:45:22
`
Hi, I'm facing an issue when compiling BWISE at step one itself, encountering the error.
I put binaries in /home/igib/visanu2/BWISE/bin PHASE ONE LAUNCHER: BWISE there was a problem with binary compilation, check logs
The OS I'm using is Ubuntu 22.04 LTS, CMAKE version 3.22.1, Make version 4.3 and GCC version 11.2.0 in a Python 3.7.13 environment.
Bwise terminated at Graph 1: Construction steps. All the log files are empty !
Bwise version used : https://anaconda.org/bioconda/bwise
`(base) jit@jit-HP-Pro-3335-MT:~/Downloads/setu$ bwise -x interleaved.fa -o bwise_assembly
*** This is BWISE - High order De Bruijn graph assembler ***
The command line was: /home/jit/anaconda3/bin/bwise -x interleaved.fa -o bwise_assembly
[Warning] bwise_assembly directory already exists, BWISE will use it.
Results will be stored in: /home/jit/Downloads/setu/bwise_assembly
[17:37:32 16/05/2020] --> Starting Graph construction and Super Reads generation...
#Graph 1: Construction...
#Current date & time Sat May 16 17:37:32 2020
bcalm -max-memory 10000 -in /home/jit/Downloads/setu/bwise_assembly/bankBcalm.txt -kmer-size 63 -abundance-min 3 -out /home/jit/Downloads/setu/bwise_assembly/out -nb-cores 20
[ERROR] There was a problem generating "/home/jit/Downloads/setu/bwise_assembly/out.unitigs.fa".
[FATAL ERROR] One or several files could not be generated.
To find out why, try Bwise --help
and/or check the logs files of the various steps of the pipeline (logs/logBloocoo, logs/logBcalm, logs/logTips, logs/logBgreat, logs/logK2000).
`
what is the difference between compacted_unitigs*.fa and dbg*.fa
If I launch Bwise.py in e.g. $HOME/1/2/3/4/5 without specifying any output directory, the output folder gets set to $HOME/1/2/3/4: this is the folder where all temporary files are created, but BWISE looks for them in $HOME/1/2/3/4/5 and crashes.
Hi!
I plan to try your assembler for my data which are composed of 3 PE libraries:
I am asking your help how to use your assembler for this kind of data.
The assembler can handle multiple libraries ?
If yes, it seems that I have to use the -x option: Does it has to be formated in interleaved format ?
Can I use -x 3 times or have to merge the 3 libraries.
Thanks for your support.
BWISE has been blocked on btrim for ~10h. It has not produced the haploid assembly
OUTPUT:
python ~/BWISE26-04/BWISE/Bwise.py -x ANature_interleave.fasta -p 5 -S 5 -K 401 -H 1 -t 45 -o /media/urbe/MyADrive/ANatureAssembly26-04-18
*** This is BWISE - High order De Bruijn graph assembler ***
Binaries are in: /home/urbe/BWISE26-04/BWISE/bin
The command line was: /home/urbe/BWISE26-04/BWISE/Bwise.py -x ANature_interleave.fasta -p 5 -S 5 -K 401 -H 1 -t 45 -o /media/urbe/MyADrive/ANatureAssembly26-04-18
[Warning] /media/urbe/MyADrive/ANatureAssembly26-04-18 directory already exists, BWISE will use it.
Results will be stored in: /media/urbe/MyADrive/ANatureAssembly26-04-18
[11:14:00 26/04/2018] --> Starting Graph construction and Super Reads generation...
[...]
The file contigs_k401.fa has been produced !
/home/urbe/BWISE26-04/BWISE/bin/bgreat -Z -g dbg401.fa -u dbg401.fa -i 1000 -o 0 -t 8 -k 401 -a 15
/home/urbe/BWISE26-04/BWISE/bin/btrim -u popped_dbg.fa -k 401 -c 45 -h 8 -o crushed_dbg.fa
Hello,
I got an error with Bloocoo
/home/alessandro/BWISE/bin/Bloocoo32: /usr/local/bin/anaconda3/lib/libstdc++.so.6: version `GLIBCXX_3.4.20' not found (required by /home/alessandro/BWISE/bin/Bloocoo32)
/home/alessandro/BWISE/bin/Bloocoo32: /usr/local/bin/anaconda3/lib/libstdc++.so.6: version `CXXABI_1.3.8' not found (required by /home/alessandro/BWISE/bin/Bloocoo32)
/home/alessandro/BWISE/bin/Bloocoo32: /usr/local/bin/anaconda3/lib/libstdc++.so.6: version `GLIBCXX_3.4.21' not found (required by /home/alessandro/BWISE/bin/Bloocoo32)
Maybe this should be checked at the installation step? I don't think by default anaconda3 will be installed.
I think anaconda3 and libstdc++ is not installed by default anyway, maybe it should be checked at install time or in a "prerequisite" section?
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