Spacemake is a modular, robust, and scalable spatial transcriptomics pipeline built in Snakemake and Python. Spacemake is designed to handle all major spatial transcriptomics datasets and can be readily configured for other technologies. It can process and analyze several samples in parallel, even if they stem from different experimental methods. Spacemake's unified framework enables reproducible data processing from raw sequencing data to automatically generated downstream analysis reports. Spacemake is built with a modular design and offers additional functionality such as sample merging, saturation analysis, and analysis of long reads as separate modules.

If you find Spacemake useful in your work, consider citing it:

Spacemake: processing and analysis of large-scale spatial transcriptomics data
Tamas Ryszard Sztanka-Toth, Marvin Jens, Nikos Karaiskos, Nikolaus Rajewsky
GigaScience, Volume 11, 2022, giac064

Documentation can be found here.

We are committed to achieving a high code coverage with unit tests. The master branch utilizes the unittest module to run spacemake with small test data sets. On the current development branches, we have switched to pytest and cover a much broader range of the code. This work is ongoing.

To run the currently implemented tests on master, run python spacemake/unittests.py. This will create a directory spacemake/_tests/ inside which a minimal spacemake directory structure will be created using spacemake init and subsequently some of the core functionality (adding genomes/species, samples, changing configuration, etc.) will be executed. All output will be logged to spacemake/_tests/run_spacemake.out.log. If you encounter any weird behavior, please make sure to include the content of this file in your ticket on the issue tracker. Thank you! ...

Spacemake is an open-source project mostly maintained by the Rajewsky lab @ MDC Berlin - so, your involvement is warmly welcome! If you're excited to join us, we recommend the following steps:

• Found a bug? Contact an admin in the form of an issue.
• Implement your idea following guidelines set by the official contributing guide
• Wait for admin approval; approval is iterative, but if accepted will belong to the main repository.

In general, you can always refer to the contribution guidelines for more details! Currently, only admins will be merging all accepted changes.

Everyone interacting in spacemake's codebases, issue trackers, and discussion forums is expected to follow the PSF Code of Conduct.