Comments (4)
the main issue should be solved. but QC for short sequences is not adequately addressed. We are looking into a better organization of the QC procedure, so will hopefully tackle this along with it.
from nextclade.
Thanks a lot @george-githinji, very good points!
we didn't think about partial sequences when doing QC or calling clades -- I'll address this ASAP.
from nextclade.
I don't view missing 5' and 3' parts as a QC issue. It likely means only a fragment was sequenced. But the QC criteria should be scaled relative to length of the fragment. Let us know how you'd expect the app to behave.
from nextclade.
One approach might be to disregard Ns within the 3' and 5' regions. This implies identifying these regions from a submitted sequence.
from nextclade.
Related Issues (20)
- Maximum Sequence Limit? HOT 2
- Web: Grey scale coloring for region/country/divison if scale not predefined in reference tree HOT 5
- How to decide if the reversionSubstitutions are valid variants or not and whether to keep them? HOT 2
- Direct Auspice SVG Download
- ENH: enlarge Visualization when more nucleotides/Codons even if mutations not fall in striclty adjacent codons but close enough to need a whole view. HOT 2
- Updating certain parts of the JSON file output from Map to Array HOT 2
- linux-musl artefact gets slower rather than faster when parallelizing in contrast to gnu HOT 5
- Parsing PCR primers HOT 1
- how many SARS-COV-2 sequences can nextclade handle in a MSA file? HOT 8
- Is there any example for handling HIV data? HOT 2
- Web crash: The target <seqName> could not be identified in the dom HOT 2
- Show "browser not supported HOT 1
- Show "browser not supported" modal only once per session HOT 3
- if the qc.overallStatus of my sequences are mediocre, can we keep them for next step analysis? HOT 5
- web-based nextclade issue when using another reference HOT 8
- web(minor): when customizing dataset files, it always says "pasted sequences" even if the field is for tree HOT 2
- I upload 1490 sequences to nextclade, and upload to auspice.us, why it shows me 4255 sequences?
- Allow suppression of ` |(reverse complement)` suffix in header of alignment output HOT 2
- how many genomes can nextclade handle? HOT 1
- PCR primer mutation functionality[v3] HOT 13
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