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fastqc's Introduction

###private fork of fastqc (0.10.0)

added a read argument for unaligned bam files. probably breaks other file types, but I don't care.

#####call with: fastqc-csf/fastqc --read {d} file.bam

where {d} is:

  • 0: unaligned bam file with one read only (4 in flags)
  • 1: unaligned bam file with paired end reads. Takes only 1. read
  • 2: unaligned bam file with paired end reads. Takes only 2. read

#####build:

  1. ant deploy.
  2. copy the fastqc-csf folder to your destination.

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fastqc's Issues

quality per sequence performance

This is just a question about performance.

Do you analyse each reads/each quality score to make the per base quality box plot ? I wonder how fast is fastqc , even with multi thread !

FastQC ignoring TruSeq adaptors?

I noticed that the contaminant_list has the right TruSeq adaptors but FastQC can't recognize partial adaptors that sit at the 5' ends of reads. I was wondering what you think of it.

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