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redeemV

Introduction

ReDeeM: single-cell Regulatory multi-omics with Deep Mitochondrial mutation profiling. ReDeeM is a single-cell multiomics platform featuring ultra-sensitive mitochondrial DNA (mtDNA) variant calling and joint RNA+ATAC profiling. ReDeeM enables fine-scale lineage tracing at single cell level, allowing for subclonal and phylogenetic analyses, with simultaneous integrative analyses of cell-state and gene regulatory circuits.

The analytical pipelines for ReDeeM analysis includes two parts:

  • redeemV is set of in-house Bash pipeline and python scripts for mapping and deep mitochondrial mutation calling. (This page, Input from rawdata)
  • redeemR is an in-house R package for downstream lineage tracing and single cell integrative analysis. (Input from redeemV)

redeemV is a streamlined pipeline taking advantage of endogenous unique molecular identifier (eUMI) for consensus-based error correction in single-cell mitochondrial DNA mutation detection. Github fig variant calling strategy

Installation and usage

redeemV includes a set of ready-to-use Bash pipeline and Python scripts

git clone https://github.com/chenweng1991/redeemV.git

Please check the tutorial (A small set of example fastq files are included)

Additional documentation

Citation

Please check out our study of human hematopoiesis using ReDeeM Deciphering cell states and genealogies of human hematopoiesis

Contact

If you have any questions or suggestions, please feel free to contact us. Feedbacks are very welcome! (Chen Weng, [email protected])

redeemv's People

Contributors

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redeemv's Issues

Multiple 10x multiome reactions

Dear Chen,

Thanks again for making this wonderful tool and pipeline available. I have run the redeemV pipeline smoothly and get all expected results. One issue I have, however, is that I have multiple 10x multiome reactions. Thus, after running the pipeline, have multiple QualifiedTotalCts and RawGenotypes files from each run. Correct me if I am wrong, but I don't think I can simply concatenate the files because of the potential shared barcodes from different cells from different 10x reactions. I wonder if you could provide some guidance on where and how to modify the redeemV output files from different reactions so that they can be combined/pooled for the downstream redeemR analysis.

Thank you,
Li

empty files

Hi,
I'm running redeemV on 10x scRNA/ATAC from human but I get this error and files are empty.
what could be the issue?

rror in read.table(RawGenotypesFile) : no lines available in input Execution halted Traceback (most recent call last): File "/home/i439h/projects/heroes-aya-pools/AG_Thongjuea/Software/bulkWGS/Sadeghi/redeemV/REDEEM-V/mitoConsensus//RemoveStrandBias.py", line 16, in <module> with open(StrandBiaseBlackList_file) as f: FileNotFoundError: [Errno 2] No such file or directory: '/home/i439h/projects/heroes-aya-pools/AG_Thongjuea/Result/bulkWGS/mitochondria/redeem/pool3/final/StrandBiaseBlackList'

Best
Iman

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