RomicsProcessor is an R package that can be used to analyze omics data. The package provides a structured R object to store the data, allowing for reproducible data analysis. The package also supports creating analytical pipelines from previously processed objects and applying these pipeline to other objects. This allows for rapid development and reuse of bioinformatics methods.
To install the package in R please first make sure devtools is installed
install.packages("devtools")
When devtools is installed, run the following command to install RomicsProcessor and its dependencies
devtools::install_github(“PNNL-Comp-Mass-Spec/RomicsProcessor”)
Alternately, download the built package and install the package manually.
We recommend the installation of the Bioconductor package 'sva' if batch corrections are needed and of 'pmartR'.
The folder /Example contain an R markdown file .
This example consist in an analysis of the proteome of B. cereus grown in different media. The Raw LC-MS/MS data is publicly available, on MassIVE, FTP at the following address (ftp://massive.ucsd.edu/MSV000085696/).
It was analyzed using MaxQuant set with the attached parameters .
The MaxQuant output proteinGroups.txt Can be used as data and the metadata is also provided.
The different examples dataset are accessible directly inside the package data folder.
To cite the package please use the following DOI:
Written by @GeremyClair for the Department of Energy (PNNL, Richland, WA)
E-mail: [email protected] or [email protected]
Website: https://omics.pnl.gov/ or https://panomics.pnnl.gov/
RomicsProcessor is licensed under the 2-Clause BSD License; you may not use this file except in compliance with the License. You may obtain a copy of the License at https://opensource.org/licenses/BSD-2-Clause
Copyright 2019 Battelle Memorial Institute