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Comments (6)

zhanghao-njmu avatar zhanghao-njmu commented on June 16, 2024

SCP (v0.3.0) is not the latest version. You need to update SCP and try again.

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Byronxy avatar Byronxy commented on June 16, 2024

Hi,
I get a different error

> pancreas_sub <- RunSCVELO(
+     srt = pancreas_sub, group_by = "SubCellType",
+     linear_reduction = "PCA", nonlinear_reduction = "UMAP"
+ )
'misc' slot is not converted.
'tools' slot is not converted.
Error: RuntimeError: numpy.linalg.LinAlgError: Singular matrix

I used the latest version of SCP

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qindan2008 avatar qindan2008 commented on June 16, 2024

I updated SCP to version 0.4.7.9000, and still got the same error
截屏2023-04-10 13 45 58

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DocMonster77 avatar DocMonster77 commented on June 16, 2024

pancreas_sub <- RunPAGA(srt = pancreas_sub, group_by = "SubCellType", linear_reduction = "PCA", nonlinear_reduction = "UMAP")
'misc' slot is not converted.
'tools' slot is not converted.
Error in match.arg(arg = layer, choices = Layers(object = object, search = FALSE)) :
'arg' should be one of "counts", "data", "scale.data"

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DocMonster77 avatar DocMonster77 commented on June 16, 2024

I get a different error
pancreas_sub <- RunSCVELO(

  • srt = pancreas_sub, group_by = "SubCellType",
  • linear_reduction = "PCA", nonlinear_reduction = "UMAP"
  • )
    'misc' slot is not converted.
    'tools' slot is not converted.
    Filtered out 12123 genes that are detected 30 counts (shared).
    Normalized count data: X, spliced, unspliced.
    Logarithmized X.
    computing neighbors
    finished (0:00:00) --> added
    'distances' and 'connectivities', weighted adjacency matrices (adata.obsp)
    computing moments based on connectivities
    finished (0:00:01) --> added
    'Ms' and 'Mu', moments of un/spliced abundances (adata.layers)
    computing velocities
    finished (0:00:00) --> added
    'stochastic', velocity vectors for each individual cell (adata.layers)
    computing velocity graph (using 1/12 cores)
    Error in match.arg(arg = layer, choices = Layers(object = object, search = FALSE)) :
    'arg' should be one of “counts”, “data”, “scale.data”

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zjh0613 avatar zjh0613 commented on June 16, 2024

Hello developers, when I run RunSCVELO I get an error. How did this happen and how do I fix it. Thank you
5e428bc5adef217bf0fc93cb88b279c
Next is the basic information I entered for the seruat object
160f87a1eb2db29a7de92c87f07307e

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