Comments (8)
Please check your indexed human and TE reference files. You can also check if the aligned BAM file is correctly indexed and sorted, if not, you can check your installed SAMtools version which should be higher than v1.5.
from ervcaller.
I runned ERVcaller with your test data in different servers, the ERVcaller reported same error and generated an empty vcf file, Maby you should check the file '${input_sampleID}_ERV.output', which didn't be generated as expected.
This is my code:
#!/bin/bash
conda activate R3 #activate the R3.3.2 envierment
perl /home/software/ERVcaller/ERVcaller_v1.4.pl \
-i TE_seq \
-I /home/software/ERVcaller/test/BWA/ \
-f .bam \
-H human.fa \
-T /home/software/ERVcaller/Database/HERVK.fa \
-t 2 -S 20 -G -BWA_MEM \
-l 500 \
-L 100
from ervcaller.
It works correctly on servers from many other users as well as mine. According to my experience, it usually caused by incorrect inputs. If it is easier for you, can you show the screenshots for: 1) a list of produced intermedia files (using command line: ls -lh); 2) your input paired-end reads (using command line cat); and 3) a list of generated index files for both human.fa and HERVK.fa (using command line: ls -lh).
I would suggest to use full paths and as less parameters as you can for now follow the manual of ERVcaller. For example:
perl /home/software/ERVcaller/ERVcaller_v1.4.pl
-i TE_seq
-I /home/software/ERVcaller/test/BWA/
-f .bam
-H **full paths/**human.fa
-T /home/software/ERVcaller/Database/HERVK.fa
-BWA_MEM
from ervcaller.
(base) root@zhu-PC:/media/zhu/A64E22B94E228263/clean/humman# ll
总用量 6.9G
-rwxrwxrwx 1 zhu zhu 445 9月 4 10:52 ervcaller.sh
-rwxrwxrwx 1 zhu zhu 3.1G 9月 2 17:10 human.fa
-rwxrwxrwx 1 zhu zhu 22K 9月 4 00:20 human.fa.amb
-rwxrwxrwx 1 zhu zhu 83K 9月 4 00:20 human.fa.ann
-rwxrwxrwx 1 zhu zhu 3.1G 9月 4 00:19 human.fa.bwt
-rwxrwxrwx 1 zhu zhu 781M 9月 4 00:20 human.fa.pac
-rwxrwxrwx 1 zhu zhu 11K 9月 4 00:20 nohup.out
drwxrwxrwx 1 zhu zhu 48 9月 4 12:29 TE_seq_subgenome
drwxrwxrwx 1 zhu zhu 408 9月 4 12:29 TE_seq_temp
-rwxrwxrwx 1 zhu zhu 0 9月 4 12:29 TE_seq.vcf
###############################################
(base) root@zhu-PC:/media/zhu/A64E22B94E228263/clean/humman/TE_seq_subgenome# ll
总用量 0
############################################################
(base) root@zhu-PC:/media/zhu/A64E22B94E228263/clean/humman/TE_seq_temp# ll
总用量 4.5K
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV_1.1fuq
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV1.bian
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV_1sf.fuq
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV_2.1fuq
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV.fine_mapped
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV.hf
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV.output
-rwxrwxrwx 1 zhu zhu 925 9月 4 12:28 TE_seq_ERV.output2
-rwxrwxrwx 1 zhu zhu 0 9月 4 12:29 TE_seq_ERV.output2.1
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV.TE_f
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV.TE_f2
-rwxrwxrwx 1 zhu zhu 0 9月 4 10:54 TE_seq_ERV.visualization
-rwxrwxrwx 1 zhu zhu 32 9月 4 10:54 TE_seq.type.gz
############################################
(base) root@zhu-PC:/home/software/ERVcaller/Database# ll
总用量 976K
-rw-r--r-- 1 root root 814K 9月 1 16:37 ERV_library.fa
-rw-r--r-- 1 root root 8.2K 9月 1 16:37 HERVK.fa
-rw-r--r-- 1 root root 9 9月 2 17:20 HERVK.fa.amb
-rw-r--r-- 1 root root 42 9月 2 17:20 HERVK.fa.ann
-rw-r--r-- 1 root root 8.1K 9月 2 17:20 HERVK.fa.bwt
-rw-r--r-- 1 root root 2.0K 9月 2 17:20 HERVK.fa.pac
-rw-r--r-- 1 root root 4.1K 9月 2 17:20 HERVK.fa.sa
-rw-r--r-- 1 root root 115K 9月 1 16:37 Human_TE_library.fa
#####################################################
(base) root@zhu-PC:/home/software/ERVcaller/test/BWA# ll
总用量 15M
-rw-r--r-- 1 root root 13M 9月 1 16:37 TE_seq.bam
-rw-r--r-- 1 root root 1.2M 9月 1 16:37 TE_seq.bam.bai
from ervcaller.
It looks like it stopped very early due to the alignment or format conversion steps, which mainly use BWA and SAMtools.
Can you specify the full path for your indexed human reference genome and try again? It guess it should be here /media/zhu/A64E22B94E228263/clean/humman/ on your PC.
Let me know if it is not working. With attached log file and similar screenshot.
from ervcaller.
It's worked with your test data and part of mine, here is the output and the TSD sequence is too long
MDM1.txt
from ervcaller.
That sounds good that the ERVcaller works. ERVcaller outputs all results, and the users can filter the results based on the reported genotype quality and likelihood. It is very useful when the users are working on a population. For the TSD sequence, based on the literature, long TSD existed. we currently use 500 bp for now to keep high sensitivity and accuracy. we may improve it in our next version.
from ervcaller.
Thank you for your work, you helped me a lot.
from ervcaller.
Related Issues (16)
- About input bam files HOT 2
- About required programs HOT 3
- About "xx_h1_sorted.bam" file HOT 4
- Step 2 - Improper reads onwards, bwa-mem fails to read index files
- 关于SE_MEI的问题 HOT 2
- Argument "-" isn't numeric in subtraction (-) at ERVcaller_v.1.3.pl line 1305, <OUT3> line 2. HOT 1
- failed at step 3 HOT 3
- Problem in Step 2 HOT 5
- Error unexpected end of file HOT 1
- The Requirement software HOT 1
- The help message of ERVcaller_v1.4.pl HOT 3
- Illegal division by zero HOT 8
- Error in User Manual HOT 1
- Do you have any recommended standard filter? HOT 4
- same sample, different result. HOT 8
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from ervcaller.