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License: GNU General Public License v3.0
Issue tracker repo for running Seurat on CLIP/CBE
License: GNU General Public License v3.0
Parameter | Value |
---|---|
Date | 08/12/2020 |
Time | ~23:00 |
Queue | m |
Node | ? |
Memory requested | ?1500 |
CPUs requested | ?65 |
CPUs used | 30 |
> workers
[1] 30
> tic(); combined.obj <- IntegrateData(anchorset = anchors, dims = 1:p$'n.CC'); toc(); say()
Finding integration vector weights
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Integrating data
Merging dataset 17 into 22 33
Extracting anchors for merged samples
Finding integration vectors
Finding integration vector weights
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
...
Integrating data
Merging dataset 22 33 17 36 37 35 38 39 40 1 into 6 15 18 19 14 20 7 8 10 11 9
Extracting anchors for merged samples
Finding integration vectors
Finding integration vector weights
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Integrating data
Merging dataset 24 41 23 31 29 30 28 3 21 32 25 26 27 12 13 16 into 6 15 18 19 14 20 7 8 10 11 9 22 33 17 36 37 35 38 39 40 1
Extracting anchors for merged samples
Finding integration vectors
Error in validityMethod(as(object, superClass)) :
long vectors not supported yet: ../../src/include/Rinlinedfuns.h:535
In addition: There were 50 or more warnings (use warnings() to see the first 50)
pipe broke overnight after this, so cannot see warnings().
require('devtools')
devtools::install_url('https://cran.r-project.org/src/contrib/Archive/Matrix.utils/Matrix.utils_0.9.7.tar.gz')
tic(); anchors <- FindIntegrationAnchors(object.list = ls.Seurat, dims = 1:p$'n.CC', reduction = "rpca"); toc(); say();
Computing 2000 integration features
Scaling features for provided objects
Error: Failed to retrieve the result of MulticoreFuture (future_lapply-21) from the forked worker (on localhost; PID 85677). Post-mortem diagnostic: No process exists with this PID, i.e. the forked localhost worker is no longer alive.
In addition: There were 19 warnings (use warnings() to see them)
combined.obj <- Calcq90Expression(obj = combined.obj)
Error in asMethod(object) :
Cholmod error 'problem too large' at file ../Core/cholmod_dense.c, line 105
https://github.com/satijalab/seurat/issues?q=+Cholmod+error+%27problem+too+large%27
While in local Rstudio, I can simply source from my own repo's, this does not work on CBE command line. Also, the token seems to keep changing
Error in file(filename, "r", encoding = encoding) :
cannot open the connection to 'https://raw.githubusercontent.com/vertesy/SEO/master/GO-scoring/Seurat.gene.sets.and.GO.terms.R?token=XXXXXXXXXXXXXXXXXXXXXXXXXXXX'
In addition: Warning messages:
1: no DISPLAY variable so Tk is not available
2: In file(filename, "r", encoding = encoding) :
cannot open URL 'https://raw.githubusercontent.com/vertesy/SEO/master/GO-scoring/Seurat.gene.sets.and.GO.terms.R?token=XXXXXXXXXXXXXXXXXXXXXXXXXXXX': HTTP status was '404 Not Found'
Using V8 engine 6.8.275.32-node.12
> if (TRUE) source('~/GitHub/Packages/Seurat.pipeline/elements/Differential.gene.expression.Loop.R'); create_set_Original_OutDir()
[1] "All files will be saved under the original OutDir: ~/Dropbox/Abel.IMBA/AnalysisD/Abel/SEO2/2021.04.12.rPCA.premRNA.dSample.Organoids/"
[1] "Differential.Gene.expression"
[1] "All files will be saved under 'NewOutDir': ~/Dropbox/Abel.IMBA/AnalysisD/Abel/SEO2/2021.04.12.rPCA.premRNA.dSample.Organoids/Differential.Gene.expression/"
[1] "ParentDir was defined as: ~/Dropbox/Abel.IMBA/AnalysisD/Abel/SEO2/2021.04.12.rPCA.premRNA.dSample.Organoids/"
[1] "ParentDir will be: ~/Dropbox/Abel.IMBA/AnalysisD/Abel/SEO2/2021.04.12.rPCA.premRNA.dSample.Organoids/"
[1] "Call *create_set_Original_OutDir()* when chaning back to the main dir."
[1] "Resolutions analyzed: 0.2 0.5"
[1] "All files will be saved under 'OutDir': ~/Dropbox/Abel.IMBA/AnalysisD/Abel/SEO2/2021.04.12.rPCA.premRNA.dSample.Organoids/Differential.Gene.expression/res.0.2/"
Calculating cluster 0
Calculating cluster 1
Calculating cluster 10
Calculating cluster 11
Calculating cluster 2
Calculating cluster 3
Calculating cluster 4
Calculating cluster 5
Calculating cluster 6
Calculating cluster 7
Calculating cluster 8
Calculating cluster 9
4987.281 sec elapsed
Error in `$<-.data.frame`(`*tmp*`, "combined.score", value = numeric(0)) :
replacement has 0 rows, data has 7937
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