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tlemane avatar tlemane commented on August 26, 2024

Hello,

Sorry for this issue. It seems that the maximum number of simultaneously opened files has been reached on your system. Since you have a large number of samples, kmtricks needs to open a lot of files during the merge.

You can try to increase this limit (see ulimit) and/or reduce the number of threads.

To avoid recomputing the first steps during your tests, I suggest to split the pipeline as follows:

1/ Count

kmtricks pipeline --file list_fastq_kmtricks --run-dir kmtricksDir --kmer-size 31 --hard-min 5 --mode kmer:count:bin --until count --cpr -t 12

2/ Merge

kmtricks merge --run-dir kmtricksDir --recurrence-min 10 --mode kmer:count:bin --cpr -t <nb_threads>

This way you can do several merge tries without recounting the partitions.

I hope this help.

Téo

from kmtricks.

MorillonLab avatar MorillonLab commented on August 26, 2024

from kmtricks.

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