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tlemane avatar tlemane commented on August 26, 2024

Hello,

Sorry for this issue.
The problem seems to occur at an early stage of the process in an external library which basically reads a few sequences from each input file to compute a partitioning scheme. I have never encountered this kind of problem, can you post an example of your data ? Just a few lines from one of your fastq ?

Teo

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dosshra avatar dosshra commented on August 26, 2024

Thank you @tlemane for the response. See attached file. I should mention that a toy example of 3 fastq files from a different source worked well on the same machine.
Thank you
ERR_example.fastq.txt

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dosshra avatar dosshra commented on August 26, 2024

Hello,
I Downloaded a different version of the fastq files with a limit of 1M reads. kmtricks pipeline was running successfully on 20 files. I will try up-scaling.

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dosshra avatar dosshra commented on August 26, 2024

Hello
I tried to run with all samples and data. 120 samples, 420 read files (~14GB each). kmtricks pipeline --file ./fof1 --run-dir ~/18tb/kmer_pa_bin --kmer-size 31 --mode kmer:pa:bin -t 10 --hard-min 2 2>kmtricks.err
I got these running messages:

Compute SuperK [=======> ] [09h:33m:15s]
Compute SuperK [================================> ] [01d:11h:10m:14s]
Compute SuperK [==================================================] [02d:04h:29m:01s]
Count partitions [======================Compute SuperK [==================================================] [02d:04h:29m:01s] Count partitions [==================================
================] [02d:04h:29m:01s]
Merge partitions [> ] [00:00s]

This back trace:

Backtrace:
1 0x00007f6987042520 (null) + 140091213489440
2 0x00007f69870969fc pthread_kill + 300
3 0x00007f6987042476 raise + 22
4 0x00007f69870287f3 abort + 211
5 0x00007f6987476f00 __gnu_cxx::__verbose_terminate_handler() + 192
6 0x00007f698747543c (null) + 140091217892412
7 0x00007f698747548e (null) + 140091217892494
8 0x000055bde4189705 (null) + 94274063996677
9 0x00007f6987045495 (null) + 140091213501589
10 0x00007f6987045610 on_exit + 0
11 0x000055bde424b98a (null) + 94274064791946
12 0x00007f6987042520 (null) + 140091213489440
13 0x00007f69870e57f8 clock_nanosleep + 200
14 0x00007f69870ea677 __nanosleep + 23
15 0x000055bde425d66b (null) + 94274064864875
16 0x000055bde43bd7f0 (null) + 94274066307056
17 0x000055bde4170260 main + 3312
18 0x00007f6987029d90 (null) + 140091213389200
19 0x00007f6987029e40 __libc_start_main + 128
20 0x000055bde41725e5 (null) + 94274063902181

And these error log:

[2024-04-02 08:42:46.390] [info] Run with Kmer<32> - uint64_t implementation
[2024-04-02 08:42:46.615] [info] Compute configuration...
[2024-04-02 08:42:46.616] [info] 204 samples found (408 read files).
[2024-04-02 08:43:47.104] [info] Use 169 partitions.
[2024-04-02 08:43:47.108] [info] Compute minimizer repartition...
terminate called after throwing an instance of 'std::runtime_error'
terminate called recursively
what(): Unable to open /home/user/18tb/kmer_pa_bin/counts/partition_6/TA2703.kmer
terminate called recursively
terminate called recursively
[2024-04-06 07:04:10.765] [error] Killed after receive Aborted:SIGABRT(6) signal. Demangled backtrace dumped at ./kmtricks_backtrace.log. If the problem persists, please open an issue with the return of 'kmtricks infos' and the content of ./kmtricks_backtrace.log

The file TA2703.kmer is there and about the same size of the other kmer files.
It seems that some of the first steps wee completed. How can I run kmtricks to try and complete only the missing steps?
Thank you

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dosshra avatar dosshra commented on August 26, 2024

Hello
I ran the following code:
kmtricks pipeline --file ./fof1 --run-dir ~/18TB/kmer_pa_bin --kmer-size 31 --mode kmer:pa:bin -t 10 --hard-min 2 --until count 2>kmtiks.err
This part runs successfully. And then I ran the following code:
kmtricks merge --run-dir kmer_pa_bin --mode kmer:pa:text -t 15 -v debug 2>merge_kmtrics.err
The command terminates immediately while generating the files listed below. Any help would be much appreciated
kmtricks_backtrace.log.txt
merge_kmtrics.err.txt
.

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