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DoBo: Protein Domain Boundary Prediction by Integrating Evolutionary Signals and Machine Learning 8/15/2016 Web-server ---------- http://sysbio.rnet.missouri.edu/dobo/ Installation ------------ (1) Download 'dobo.zip', 'models.zip', 'programs.zip', and 'db.zip' into a folder Download URL: http://sysbio.rnet.missouri.edu/bdm_download/dobo/downloads/ $ mkdir dobo $ cd dobo (2) Unzip the files $ unzip dobo.zip $ unzip db.zip $ unzip programs.zip $ unzip models.zip (3) Verify that the following files folders and files are present (a) bin/, lib/, sample/, scripts/, old_readme.pdf, readme.txt (this file) (b) programs/ (c) db/ (4) Configure SSPro Update all the 'install dir' and 'db dir' in 'programs/sspro4.1/configure.pl' and run SSPro configuration $ cd ./programs/sspro4.1/ $ ./configure.pl (5) Update the paths for SCRIPT_DIR, PROGRAM_DIR, MODELS_DIR, and LD_LIBRARY_PATH in the following scripts (a) bin/run-dobo-stage1.sh (b) bin/run-dobo-stage2.sh For eg. sed -i "s/\/rose\/space1\/bap54\/temp\/dobo-share\/programs/\/tmp\/dobo\/programs/g" ./bin/run-dobo-stage1.sh (6) Update the paths for BLAST_PATH, BLAST_NR_DB, BLASTMAT, CONVERT_PATH in 'scripts/generate-msa.sh' (7) Test DoBo $ ./bin/run-dobo-stage2.sh sample/test.fasta test-stg2.out (8) Verify the results with the web-server results Submit the same sequence into the web-server and ensure that you local results match the web-server results. Publication ----------- J. Eickholt, X. Deng, and J. Cheng. DoBo: Protein Domain Boundary Prediction by Integrating Evolutionary Signals and Machine Learning. BMC Bioinformatics. 12:43, 2011. Contact ------- Prof. Jianlin Cheng [email protected]
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