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shilpagarg avatar shilpagarg commented on August 14, 2024

Please see #16

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jenny315 avatar jenny315 commented on August 14, 2024

Hi,
Thank you very much, although I don't quite understand what does it means that dipasm is a proof-of-concept tool .

1.Does this mean that I can also adopt the above idea(hifiasm+pstools) instead of using diPasm?
2.For diploid fish (the haploid genome <1G), can the result of Hifiasm mentioned above be replaced by the result of Hicanu?

Thanks for any advice,
Best regards,
Jenny

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shilpagarg avatar shilpagarg commented on August 14, 2024

Yes, please use hifiasm+pstools, because it is fast, accurate, as well as user-friendly.
In theory, hifiasm and Hicanu graphs should be similar, but I have not yet tested hicanu graphs.

Let me know if you have any questions. Look forward!

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jenny315 avatar jenny315 commented on August 14, 2024

Thank you very much, I'll have a try at once.

Best regards,
Jenny

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shilpagarg avatar shilpagarg commented on August 14, 2024

hic_mapping_unitig is a subcommand in pstools. Please read instructions carefully. Thanks.

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duhuipeng avatar duhuipeng commented on August 14, 2024

Thank you

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duhuipeng avatar duhuipeng commented on August 14, 2024

image

image
dear author
I applied your test data in dipasm, However, he made the mistake and what caused it.

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duhuipeng avatar duhuipeng commented on August 14, 2024

image
/home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_1.fastq.gz) <(zcat /home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_2.fastq.gz); /home/Mpzhang/Mzhou/Biosoft/pstools resolve_haplotypes -t 64 hic_name_connection.output pgp1.asm.r_utg.gfa ./; /home/Mpzhang/Mzhou/Biosoft/pstools hic_mapping_haplo -t 64 pred_haplotypes.fa <(zcat /home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_1.fastq.gz) <(zcat /home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_2.fastq.gz) -o scaff_connections.txt; /home/Mpzhang/Mzhou/Biosoft/pstools haplotype_scaffold -t 64 scaff_connections.txt pred_haplotypes.fa ./

This is the code I run, the data comes from githup test data, the result is the picture above,Why is this happening

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shilpagarg avatar shilpagarg commented on August 14, 2024

It may be efficient to schedule a meeting. If you could write me at [email protected], that would be good.

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duhuipeng avatar duhuipeng commented on August 14, 2024

ok, I am writing now

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