Comments (10)
Please see #16
from dipasm.
Hi,
Thank you very much, although I don't quite understand what does it means that dipasm is a proof-of-concept tool .
1.Does this mean that I can also adopt the above idea(hifiasm+pstools) instead of using diPasm?
2.For diploid fish (the haploid genome <1G), can the result of Hifiasm mentioned above be replaced by the result of Hicanu?
Thanks for any advice,
Best regards,
Jenny
from dipasm.
Yes, please use hifiasm+pstools, because it is fast, accurate, as well as user-friendly.
In theory, hifiasm and Hicanu graphs should be similar, but I have not yet tested hicanu graphs.
Let me know if you have any questions. Look forward!
from dipasm.
Thank you very much, I'll have a try at once.
Best regards,
Jenny
from dipasm.
hic_mapping_unitig is a subcommand in pstools. Please read instructions carefully. Thanks.
from dipasm.
Thank you
from dipasm.
dear author
I applied your test data in dipasm, However, he made the mistake and what caused it.
from dipasm.
/home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_1.fastq.gz) <(zcat /home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_2.fastq.gz); /home/Mpzhang/Mzhou/Biosoft/pstools resolve_haplotypes -t 64 hic_name_connection.output pgp1.asm.r_utg.gfa ./; /home/Mpzhang/Mzhou/Biosoft/pstools hic_mapping_haplo -t 64 pred_haplotypes.fa <(zcat /home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_1.fastq.gz) <(zcat /home/Mpzhang/HpDu/dipasm/DipAsm/hi-c/hic_2.fastq.gz) -o scaff_connections.txt; /home/Mpzhang/Mzhou/Biosoft/pstools haplotype_scaffold -t 64 scaff_connections.txt pred_haplotypes.fa ./
This is the code I run, the data comes from githup test data, the result is the picture above,Why is this happening
from dipasm.
It may be efficient to schedule a meeting. If you could write me at [email protected], that would be good.
from dipasm.
ok, I am writing now
from dipasm.
Related Issues (20)
- can DipAsm use on singularity? HOT 2
- what's the recommended memory to run DipAsm? HOT 1
- permission denied of installment HOT 2
- Can Dipasm be used for plant genomes? HOT 2
- DipAsm HOT 1
- Fast and accurate chromosome-scale phased assembly HOT 7
- gzip: stdout: Broken pipe HOT 1
- The result of the run is returned, but "scaffold_connection.txt" is 0 in the result file HOT 2
- divergent genome size between two haplotypes after using pstools HOT 1
- memory: core dumped at haplotype_scaffold HOT 6
- Pstools pre_hap1 or pre_hap2 HOT 5
- Scaffolded by 3D-DNA or HiRise in unphased assembly
- Installing using singularity HOT 2
- It is hard for me to install through your instruction HOT 1
- Quality control for hifi and hic data HOT 1
- pstools_1 "Segmentation fault (core dumped)"
- install problem
- Applying to contigs or chromosome-level scaffolds
- CommandNotFoundError HOT 2
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from dipasm.