Files and programs related to my dissertation Meeting 9/3/15- %probably need more than technical replicates - need some kind of information to contsrain the problem start with understanding the modern normalization technologies limma DESeq2 %use multivariate response? Take advantage of the multiple analytes measured simultaneously %Look up multivariate calibration in the anlytical chemistry literature %Measurement error models- "Errors-in-varaibles" %Measurement error in non-linear models with mean variance issue in sequencing data %There will be at least one question on measurement error models %do probe features add information to help with calibration %use normalization comparison paper to motivate the current exercise (avoid using outcome) %Start by writing down model and figureing out how to estimate the parameters %figure out the relationship between x and y without only using y!!
Major take Home: Get to know the calibration problem.