This is an implementation of the Mitochondrial Event Localiser as a plugin for Fiji/ImageJ.

Copy the MEL_Fiji_Plugin.jar into the the plugins folder of where you extracted Fiji. (e.g. C:<path-to-Fiji>\Fiji.app\plugins\MEL_Fiji_Plugin.jar)

This plugin relies on the 3D ImageJ Suite, which can be downloaded here. Note the version the current MEL plugin relies on is 4.0.1 which can also be downloaded from this repository (the file is called mcib3d-core-4.0.1.zip). Extract and copy it to the same plugins folder.

This plugin is still under development, it should however be mostly functional even though the documentation is currently limited. We hope to improve the user friendliness of the system and have it entirely refined by the end of 2022.

Currently, the plugin can be run by selecting it under "Plugins>MEL Process" in the Fiji menu system.

If you face issues with the use of this plugin, please email me at rptheart(at)sun.ac.za

This plugin relies on the mcib3d-suite to work. The appropriate version can be found as a zip file in this repository. Please extract the zip file to the Fiji plugins folder as explained above under Installation.

If you use this plugin, please cite the following paper:

Theart RP, Kriel J, du Toit A, Loos B, Niesler TR (2020) Mitochondrial event localiser (MEL) to quantitatively describe fission, fusion and depolarisation in the three-dimensional space. PLoS ONE 15(12): e0229634. https://doi.org/10.1371/journal.pone.0229634