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paper2's Introduction

paper2

development setup

The following is not necessary to run if you are following along with the tutorial. Only run these commands if you are publishing a new version or release of this document!

conda env create -n protocols --file qiime2-$RELEASE-py36-$ARCH-conda.yml
conda activate protocols
conda install -c conda-forge songbird redbiom -y
conda install -c bioconda bowtie2 -y
pip install https://github.com/knights-lab/SHOGUN/archive/v1.0.7.zip
pip install https://github.com/qiime2/q2-shogun/archive/master.zip
conda install cytoolz -y
conda install -c conda-forge sphinx awscli -y

building

export AWS_ACCESS_KEY_ID='MY_KEY_ID'
export AWS_SECRET_ACCESS_KEY='MY_SECRET_ACCESS_KEY'

make html

# once you have spot-checked the resulting HTML deploy the build:
make deploy

# and commit/push the latest `docs/` dir changes to this repo
git add docs/*
git commit -m "Some clever message here"
git push origin master

paper2's People

Contributors

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paper2's Issues

Songbird installation in conflict with QIIME 2 release 2021.8

Bug Description
Instructions for songbird installation on top of the latest QIIME 2 distribution cause errors. It appears the latest version of songbird is at odds with the QIIME 2 environment's Python version.

Steps to reproduce the behavior

  1. Install QIIME 2 core distribution (release 2021.8) in conda virtual environment
  2. Follow tutorial instructions to install songbird in the environment: conda install songbird -c conda-forge
  3. See error screenshot below

Screenshots
Screenshot 2021-11-09 at 14 57 26

Computation Environment

  • OS: macOS Big Sur
  • QIIME 2 Release 2021.8, in conda environment

--p-n-jobs should be renamed for qiime2-2021.8

Bug Description
The given command for computing diversity uses an outdated flag --p-n-jobs. The latest release of QIIME 2 (core 2021.8 distribution) renamed this to --p-n-jobs-or-threads.

Steps to reproduce this behavior

  1. Follow the tutorial
  2. Error out running qiime diversity core-metrics-phylogenetic, found twice in the alpha diversity section

BUG: metadata file from qiita is missing crucial columns

Bug Description
The metadata file in qiita must have been swapped since the last time these docs were built/tested.

The tutorial fails to run now because (most) metadata columns are missing. These are the only columns that I am finding right now in the file 81253_mapping_file.txt:
sample_name platform qiita_prep_id run_prefix

Steps to reproduce the behavior

  1. Follow the tutorial
  2. The moment you use the metadata file for anything, it fails on missing metadata columns

I am assuming that those columns were once present in the original metadata file, since this tutorial worked previously.

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