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Home Page: https://github.com/pblumenkamp/Curare
License: GNU General Public License v3.0
Curare - A customizable and reproducible analysis pipeline for RNA-Seq experiments
Home Page: https://github.com/pblumenkamp/Curare
License: GNU General Public License v3.0
Rename repository to Curare
In GitLab by @[email protected] on Apr 23, 2018, 17:11
The database path must be relative. Absolute paths don't work at the moment.
In GitLab by @[email protected] on Apr 11, 2018, 16:54
DESeq2 should also save a normalized count table
In GitLab by @[email protected] on Mar 30, 2020, 11:32
Automatic preprocessing
The original issue
Id: 22
Title: Werden die ungemappten reads weggeschmissen?
could not be created.
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TODO
The original issue
Id: 26
Title: [DESeq2] Empty first page in correlation heat map
could not be created.
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TODO
In GitLab by @[email protected] on Jun 5, 2018, 09:54
Each run should save the version of each tool
The original issue
Id: 23
Title: readxplorer will relativen pfad haben. hier möglicher workaround
could not be created.
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TODO
In GitLab by @[email protected] on Apr 23, 2018, 12:23
Optional settings in module config files should have a default value.
The original issue
Id: 30
Title: Sample name must not end with '_unmapped.bam'
could not be created.
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TODO
The original issue
Id: 19
Title: unzip module
could not be created.
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TODO
In GitLab by @[email protected] on Apr 11, 2018, 16:49
Switch from command line parameters to yaml config file
The original issue
Id: 11
Title: Bug or Feature: Screaming Files
could not be created.
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TODO
In GitLab by @[email protected] on Jul 4, 2018, 13:35
Only related rules should restart after modifying the pipeline config.
In GitLab by @[email protected] on Jan 17, 2019, 12:54
Add STAR as an additional mapping tool
In GitLab by @[email protected] on Apr 11, 2018, 16:52
Create a setup for conda and make it downloadable from a conda channel
The original issue
Id: 35
Title: tests/resources/run.sh falscher Zugriff auf Array
could not be created.
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TODO
The original issue
Id: 1
Title: add ReadXplorer rule
could not be created.
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TODO
In GitLab by @[email protected] on Apr 23, 2018, 11:48
A module should have the possibility to have library files or scripts which have to be copied to the results folder too.
In GitLab by @rmueller on Jun 14, 2018, 11:32
for i in `ls ../../*/*/analysis/*/mapping/logs/bowtie2_mapping.*.log`; do
echo -n $i" " >> tmp/mappings.log
tail -n+2 $i | sed -e 's/^[^0-9]*\([0-9]\{1,\}\) (\([0-9.]\{1,\}\)%).*/\1\t\2/' | sed -e 's/^\(.*\)%.*$/\1/' | tr '\n' '\t' | sed -e 's/\t$//' >> tmp/mappings.log
echo >> tmp/mappings.log
done
In GitLab by @[email protected] on Jun 8, 2018, 11:40
Files in config and groups file should be checked
In GitLab by @[email protected] on Apr 23, 2018, 17:48
Intermediate file of ReadXplorer import won't be deleted at the moment.
In GitLab by @[email protected] on Apr 11, 2018, 16:53
R scripts should save an r-dump after running
In GitLab by @rmueller on Jun 7, 2018, 15:10
In GitLab by @[email protected] on Apr 23, 2018, 11:48
Integrate DESeq2 as an analysis module
In GitLab by @[email protected] on Apr 23, 2018, 17:09
Add optional settings for bowtie2 module like local or global alignment or sensitivity.
In GitLab by @[email protected] on Apr 11, 2018, 16:58
Integrate a CI-runner for automatized testing
Adding support for 3rd generation sequencing files and adding mapping tools for long reads
The original issue
Id: 32
Title: YAML loader is deprecated
could not be created.
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TODO
In GitLab by @cblume on Oct 15, 2019, 15:06
Merges conda_integration -> development
Conda-Integration vollständig. Tests laufen alle durch und brechen, wie zu erwarten, erst bei readexplorer ab.
In GitLab by @cblume on Oct 17, 2019, 12:21
Merges segemehl_module -> development
Segemehl-Modul läuft bei mir, wie gehabt, durch bis readexplorer.
Auch die Konvertierung zu TSV ist bereits vorhanden und ein Excel-Sheet wird ebenfalls erstellt. Allerdings sollte da nachgeschaut werden, ob das Ergebnis so ausreichend ist.
In GitLab by @[email protected] on Apr 11, 2018, 16:51
Create a requirements file.
Current known requirements:
Minimal:
All (Minimal plus ...):
The original issue
Id: 34
Title: Typo: Ressources
could not be created.
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TODO
In GitLab by @[email protected] on Apr 11, 2018, 16:49
Add rule for FastQC/MultiQC
In GitLab by @cblume on Oct 24, 2019, 09:57
Merges conda_versions -> development
Wie besprochen:
Die Datei snakefiles/misc/parse_versions
enthält eine Snakemake-Rule. Diese wird, zusammen mit den anderen Subworkflows, innerhalb von curare.py
in die Datei Snakefile
kopiert.
Diese Rule führt das Skript dge_pipeline/lib/parse_versions.py
aus (welches im Laufe des Workflows nach output_dir/snakemake_lib/global_scripts
kopiert wird). Dieses Skript ordnet, mittels Kommentaren mit den Modulnamen in den jeweiligen Environment-YAML-Dateien (<module>/lib/conda_env.yaml
), die gehashten Environments den Modulen zu. Danach wird zu jedem Modul die Datei output_dir/.snakemake/conda/<environment_hash>/conda-meta/history
geparsed und die primären und sekundären Abhängigkeiten, gruppiert nach Modul, in versions.yaml
geschrieben. Diese Datei wird letztendlich unter output_dir/.report/
abgelegt.
In GitLab by @rmueller on Jan 21, 2020, 11:02
Die DESeq2 summary table muss überarbeitet werden. Zur Zeit filtert sie automatisch nach einem im Skript deklarierten Cutoff. Die einzelnen Schritte sollten auseinanderge"friemelt" werden. Des Weiteren soll nicht nur die gefilterte Summary table erstellt werden, sondern auch eine mit allen Features.
The original issue
Id: 25
Title: [DESeq2] Changing colors in featCount assignment plot
could not be created.
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TODO
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