polishing strategy and rounds about nextpolish HOT 3 OPEN

Yes, see our paper, Hu, Jiang, et al. "NextPolish: a fast and efficient genome polishing tool for long read assembly." Bioinformatics (Oxford, England) (2019)

from nextpolish.

Thanks for quickly reply. Your paper mainly focus on short reads corrections and documented that two rounds is good enough, so I plan to perform one round PacBio long-reads + two round short-reads correction using wtpos-cns, and finally use NextPolish to do two round short-reads correction. Will it work?
The assembly is ~2Gb and short-reads are about 100 Gb. My severs has ~330 Gb memory, is it enough to run NextPolish?

from nextpolish.

The memory is enough, NextPolish does not require much memory. I do not test wtpos-cns, for NextPolish, two round short-reads correction is enough, but if you want a more accurate assembly, you can try more iterations.

from nextpolish.