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Container builders for NCBI Utilities

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docker's Issues

Docker hub image (ncbi/blast/2.13.0) has signature-related error

Hi,
I've been using your image in my flask app for a while now, so thanks for making that possible! When I try to build from your ncbi/blast/2.13.0 image on docker hub, however, I suddenly get:
GPG error: https://packages.cloud.google.com/apt cloud-sdk-buster InRelease: The following signatures couldn't be verified because the public key is not available: NO_PUBKEY B53DC80D13EDEF05
whereas the same thing worked when my colleague built from this repo instead, so perhaps you need to update the docker hub image?
/ M

docker build erroneous with edirect 15.0

When using the Dockerfile to build a container with edirect version 15.0 downloading and integration of the gzipped binaries of transmute, rchive & xtract fails in edirect/setup.sh. Therefore the binaries are missing in the image.

In addition, nquire in the resulting image will not work for URLs with https/TLS. That means that nquire is not able to query the eutils which renders the edirect tools mostly non-functional.

I suspect that the malfunctioning of nquire is also the cause of transmute, rchive & xtract not being integrated into the image.

Example:

docker run --rm -it edirect esearch -db pubmed -query "systematic review[ti]"
curl: (60) SSL certificate problem: unable to get local issuer certificate
More details here: https://curl.haxx.se/docs/sslcerts.html

curl failed to verify the legitimacy of the server and therefore could not
establish a secure connection to it. To learn more about this situation and
how to fix it, please visit the web page mentioned above.
 ERROR:
 curl command failed ( Thu May  6 18:04:37 UTC 2021 ) with: 60

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi -d retmax=0&usehistory=y&db=pubmed&term=systematic%20review%5Bti%5D&tool=edirect&edirect=15.0&edirect_os=Linux&email=root%40a73e729c6207
 WARNING:
 FAILURE ( Thu May  6 18:04:36 UTC 2021 )

nquire -url https://eutils.ncbi.nlm.nih.gov/entrez/eutils/ esearch.fcgi -retmax 0 -usehistory y -db pubmed -term "systematic review[ti]" -tool edirect -edirect 15.0 -edirect_os Linux -email root@a73e729c6207
EMPTY RESULT

SECOND ATTEMPT
[...]

FATAL: kernel too old

Hi,
I met this above error when I was doing docker run ncbi/blast.
my docker version is 1.7.0.
Any idea what is going wrong?
Thank you.
Chen

magicblast docker image returning "encryption failed while validating token within cryptographic module"

Hello,

I'm trying to run magicblast from the docker image, but I'm getting a certification verification issue.

โœ— docker run -v /Users/tt/code/mblast/sample:/job ncbi/magicblast magicblast -db /job/sequence.fasta -sra SRR597644 -no_unaligned -splice F -num_threads 8 -out /job/results.sam

Error: (2108.2) [magicblast] VDB 3.0.7: 2024-03-12T18:59:31 . sys: encryption failed while validating token within cryptographic module - Verification issue 0x4008 for this certificate: (  cert. version     : 3  serial number     : 00  issuer name       : C=US, O=The Go Daddy Group\, Inc., OU=Go Daddy Class 2 Certification Authority  subject name      : C=US, O=The Go Daddy Group\, Inc., OU=Go Daddy Class 2 Certification Authority  issued  on        : 2004-06-29 17:06:20  expires on        : 2034-06-29 17:06:20  signed using      : RSA with SHA1  RSA key size      : 2048 bits  basic constraints : CA=true  )

Error: (2108.2) [magicblast] VDB 3.0.7: 2024-03-12T18:59:31 . sys: mbedtls_ssl_get_verify_result for 'locate.ncbi.nlm.nih.gov' returned 0x4008 ( !! The certificate is not correctly signed by the trusted CA  !! The certificate is signed with an unacceptable hash. )

BLAST query/options error: The provided SRA accession 'SRR597644' does not exist
Please refer to the BLAST+ user manual.

I get the same issue when I run "ncbi/magicblast" on an AWS Fargate instance too. Any ideas what could be wrong?

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