Tick the Multi-Template-Matching AND IJ-OpenCV update site of the Fiji udpater.
A new entry will show up in the Plugin Menu (all the way down) after restarting Fiji.
See how to activate an update site.

You can also do a manual installation by copying the files in the right place.
This can be useful if you would like to use a previous version that is not available via the update site, but which is archived in the releases tab.
You still need to tick the IJ-OpenCV update site in the Fiji updater to install the dependencies.

Then you can download the files either on the main page above this readme, by clicking the green button with the arrow pointing down Code then Download ZIP.
You can download the zip of previous versions on the release tab, below asset select Source code (zip).
Unzip the zip file, and on Windows, drag the folder Fiji.app from the unzipped directory, and drop it on the directory Fiji.app of your existing Fiji installation.
The idea is to merge both Fiji.app directories, in Windows this drag and dropping will automatically copy the files in the corresponding subdirectories.
Restart Fiji then.
If the drag and dropping does not work for you, you just have to copy the files from the unzziped directory to the corresponding directories in your Fiji installation.
If some directories (ex: Lib in Fiji.app/jars) does not exist, just create them.

Template matching is an algorithm that can be used for object-detections in images.
The algorithm computes the probability to find one (or several) template images provided by the user.
See the wiki section for the documentation, or the website of the project.

You can find a similar implementation in:

• Python
• KNIME relying on the python implementation

Playlist on YouTube

If you use this implementation for your research, please cite:

Thomas, L.S.V., Gehrig, J. Multi-template matching: a versatile tool for object-localization in microscopy images.
BMC Bioinformatics 21, 44 (2020). https://doi.org/10.1186/s12859-020-3363-7

Download the citation as a .ris file from the journal website, here.

This plugin is using OpenCV in Fiji thanks to IJ-OpenCV.

see also:
Domínguez, César, Jónathan Heras, and Vico Pascual. "IJ-OpenCV: Combining ImageJ and OpenCV for processing images in biomedicine." Computers in biology and medicine 84 (2017): 189-194.

The content of this wiki (including illustrations and videos) is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

As a derived work of IJ-OpenCV, the source codes are licensed under GPL-3.

This work has been part of the PhD project of Laurent Thomas under supervision of Dr. Jochen Gehrig at ACQUIFER.

This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 721537 ImageInLife.

Dataset available on Zenodo

Image courtesy Jakob Gierten (COS, Heidelberg)
Dataset available on Zenodo