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mourisl avatar mourisl commented on July 30, 2024

Can you please show me the command line you use? Can you see anything output to the result file specified from -o option (default output file is rascaf.out).

I'll create and upload a sample data set later.

Thanks.

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peterdfields avatar peterdfields commented on July 30, 2024

The command I'm using is the same as in the sample,

rascaf -b A.bam -f assembly.fa -o A

The A.out file begins as follows:

Contigs
0: scaffold10000_size206 1 206
1: scaffold10001_size206 1 206
2: scaffold10002_size206 1 206
3: scaffold10003_size206 1 206
4: scaffold10004_size206 1 206
5: scaffold10005_size206 1 206
6: scaffold10006_size206 1 206
7: scaffold10007_size206 1 206
8: scaffold10008_size206 1 206
9: scaffold10009_size206 1 206
10: scaffold1000_size26079 1 124
...

And continues until the end which is:

116307 scaffold_j128_1 99476 (18240 19653): (116308 scaffold_j128_1 99477 (19704 21040)):(1 2)
116308 scaffold_j128_1 99477 (19704 21040): (116307 scaffold_j128_1 99476 (18240 19653)):(2 2)
116309 scaffold_j128_1 99477 (21097 21124):
116310 scaffold_j128_1 99477 (21205 22036):
116311 scaffold_j128_1 99477 (22342 22499):
116312 scaffold_j128_1 99477 (22635 26001):
command line: /home/peter/bioinformatics/Rascaf/rascaf -b

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mourisl avatar mourisl commented on July 30, 2024

It seems the error happens when it trying to resolve the path to the alignment file. I've uploaded a more robust version. Can you give it a try?

From the output, are you also using the verbose mode?

Thanks

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peterdfields avatar peterdfields commented on July 30, 2024

The new version completed without error! The previous error was generated with the verbose mode flag included. Thanks for your help.

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