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rsharris avatar rsharris commented on September 6, 2024

I should add that when I run the example, it reports that a proportion of 1.0 for each and every contig. That seems really strange -- it would be strange example. How can I know whether this is expected or if instead it's an indication somethings wrong with my installation of the program?

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rsharris avatar rsharris commented on September 6, 2024

In the current readme, the threshold output by discoverY.py is described as "proportion_shared_with_female". But I think it is really "proportion_NOT_shared_with_female".

Thus values closer to 1 mean a contig is more likely to be from Y.

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deilepaita avatar deilepaita commented on September 6, 2024

Agree!

Output of DiscoverY in README.md should be corrected to "proportion_NOT_shared_with_female", because after running DiscorerY, contig file has the following header: '>Sc0000000 7492748 0.012910911534003885 102.0'; while the printed results in the terminal are:
'No. of contigs seen so far: 1
Current contig ID is : Sc0000000
Median is: 102.0
Total No. of k-mers from this contig: 7492732
No. of k-mers not shared with female: 96738
Proportion is: 0.012910911534003885'

Another correction that should be made is the description on how to calculate k-mers from male reads, because it indicates:
"cd dependency
ln -s ../data/female.fasta #make sure the correct reads file is provided to DSK
./run_dsk_Linux.sh r1.fastq 25"
which is misleading for new users. Why do the user needs to soft link female.fasta to dependency if it is completely unnecessary for running DSK?

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