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These files contain the raw gut microbiota data and corresponding analysis code. There are totally 3 groups of C57BL/6J male mice, and they are Control grop (normal diet + saline), Model group (high fat diet + saline) and L. paracasei intervention group (high fat diet + L. paracasei). We performed 8 mouse assays for each group of experiments. The gut microbiota determination is performed at GUHE Info technology Co., Ltd (Hangzhou, Jiangsu, China). Further information can refer to our manuscript "Lactobacillus paracasei IMC 502 ameliorate type 2 diabetes by mediating gut microbiota-SCFAs-hormone/inflammation pathway in mice".