NextDenovo is a string graph-based de novo assembler for long reads (CLR, HiFi and ONT). It uses a "correct-then-assemble" strategy similar to canu (no correction step for PacBio Hifi reads), but requires significantly less computing resources and storages. After assembly, the per-base accuracy is about 98-99.8%, to further improve single base accuracy, please use NextPolish.

We benchmarked NextDenovo against other assemblers using Oxford Nanopore long reads from human and Drosophila melanogaster, and PacBio continuous long reads (CLR) from Arabidopsis thaliana. NextDenovo produces more contiguous assemblies with fewer contigs compared to the other tools. NextDenovo also shows a high assembly accurate level in terms of assembly consistency and single-base accuracy.

• DOWNLOAD
  click here or use the following command:
  wget https://github.com/Nextomics/NextDenovo/releases/download/v2.5.0/NextDenovo.tgz

  Note: If you get an error like version 'GLIBC_2.14' not found or liblzma.so.0: cannot open shared object file, Please download this version.

• REQUIREMENT

  • Python (Support python 2 and 3):
    • Paralleltask

• INSTALL

pip install paralleltask
tar -vxzf NextDenovo.tgz && cd NextDenovo

• TEST
  nextDenovo test_data/run.cfg

• Quick Start - no experience required, download and assemble now
• Tutorial - step by step introduction to assemble the HG002 genome
• FAQ - frequently asked questions
• Parameter Reference - a detailed introduction about all the parameters
• Cite - if you get a good assembly with NextDenovo, please cite it

You can track updates by tab the Star button on the upper-right corner at this page.

The complete user documentation is available here.