Set of scripts for analyzing NGS data
BEDtools Version 2.17.0
Bcftools Version 1.2
BWA Version 0.7.12
FastQC Version 0.11.5
fastQValidator Version 0.1.1a
GATK Version 3.4.0
Kraken Version 0.10.5
Picard Version 1.134
Prinseq-lite.pl Version 0.204
Pigz Version 2.3.3
Qualimap Version 2.1.1
Samtools Version 1.2
SnpEff Version 4.1
Vcftools Version 0.1.126
The UVP requires at least 100GB RAM and up to 100GB storage space to run locally. Insatlling the UVP on your local machine is straight forward. Clone the entire repository, and download the specific version of each of the third party tool listed above into the 'Local Directory Path'/uvp/bin folder . You will need to edit the config.yml file in the 'Local Directory Path'/uvp/bin folder to point to the correct directory and file paths of all the scripts and tools listed there in.
You will run the UVP using command line prompts, by invoking the UVP module in the 'Local Directory Path'/uvp/scripts directory:
'Local Directory Path'/uvp/scripts/UVP -q 'input fastq' -r 'path to H37Rv reference genome fasta file' -n 'sample name' -q2 'paired fastq file' -a -v