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When i read the documentation when doing the gibson assembly it looked like i could expand the number of columns for fragments from 4 to 5 (including an empty one in between with a “-“) in the "reaction excel". For the multifragment one one or two columns extra would be handy for the extra (and differing) amounts of water you have to add. Especially, if it contains a mix reactions that comprise only one insert and reactions with multiple inserts. Alternatively, is it possible to have a column + and adjacent column that specificies the exact volume of a reagent / buffer (i.e. water) to add? (so that you can specify for one reaction water = 2 and another water = 8 etc).

So this is either a bug report or a feature request :-)

The Excel parser assumes that every target reaction has a unique (ID + subID) which is used as a key for the internal dict. If the user re-uses target IDs, only one of the reactions is processed and the others are silently dropped.

ToDo: check for duplicate IDs and raise Exception

running:

C:\EvoData\userInput\Cherrypicking>c:\python27\scripts\assemblysetup.py -dialogs -i targetlist_PCR.xlsx -src partslist.xls -o tmp.gwl

gives this error:

IndexFileError raised in assemblysetup.py line 126.
Reason: volume definition "template" does not match any source column

cherrypick_list.xlsx
dest_list.xlsx

when using distribute.py the following error is generated:

"TypeError raised in distribute.py line 114.

Reason: cannot concatenate 'str' and 'exceptions.KeyError' objects."

Used on command line:
c:\python\scripts\distribute.py -i distribute20151120.xls -scr partslist.xls

No work list is generated. Files used (.xls version, not the .xlsx uploaded here due to github restrictions)

distribution20151120.xlsx

partslist.xlsx