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mrna_meth_decon's Introduction

mrna_meth_decon

NOTE: If you don't want to build the Docker image yourself, directly go to the last section.

mrna_meth_decon is available in dockerhub

Create a Docker image

# cd <any root directory>, In our case -
cd ~/

git clone https://github.com/ashwini-kr-sharma/mrna_meth_decon.git && cd mrna_meth_decon
docker build -t ashwinikrsharma/mrna_meth_decon .
docker push ashwinikrsharma/mrna_meth_decon

Explore the Docker container

docker run --rm -it ashwinikrsharma/mrna_meth_decon:latest bash

# A bash terminal inside the Docker container should open

# Start R
R

# Inside the R console, identify if the deconvolution packages are present
ip = as.data.frame(installed.packages()[,c(1,3:4)])
ip = ip[is.na(ip$Priority),1:2,drop=FALSE]

mypacakages = c('DeconRNASeq','EpiDISH', 'estimate', 'ica', 'fastICA', 'NMF', 'fgsea', 'enrichR', 'immunedeconv', 'deconica', 'EDec', 'MeDeCom', 'medepir', 'CellMix')

# All available !
ip[ip$Package %in% mypacakages,]

# Any missing !
mypacakages[! mypacakages %in% ip$Package]

# Exit R
q()

# Exit container bash
exit

Available algorithms

(1) immunedeconv
(2) estimate
(3) CellMix
(4) DeconRNASeq

(5) EpiDISH
(6) EDec
(7) medepir

(8) ica
(9) deconica
(10) fastICA
(11) NMF

Connecting this docker image with ShinyCompExplore and running the app

ShinyCompExplore is a Shiny app that aids in a user-friendly analysis and visualization of various deconvolution algorithms.

cd ~/

# In case you didn't build the Docker image following the steps above,
# you can directly pull the built image from dockerhub

docker pull ashwinikrsharma/mrna_meth_decon:latest

git clone https://github.com/ashwini-kr-sharma/ShinyCompExplore.git

docker run --rm -it -p 3838:3838 -v ~/ShinyCompExplore:/srv/shiny-server/ ashwinikrsharma/mrna_meth_decon

# Wait for few seconds for the shiny app to start

Once you see a message Starting listener on http://[::]:3838, go to your browser (Chrome, Firefox etc) and type in the address bar localhost:3838, wait for some time for the page to load. Now, you should now be able to see the ShinyCompExplore app.

Demo data is made available in ~/ShinyCompExplore/data/demo, that can be used to test the app.

Raise a Github issue in case you face any problems !!

mrna_meth_decon's People

Contributors

ashwini-kr-sharma avatar

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