Do you have a lot of fluorescent beads and want to detect them accurately and quantify their time course? Try BeadQuant, which is an easy-to-use and function-rich tool based on MATLAB.

The paper draft is under preparation. The user guide is Bead 0.5.docx in the top folder.

The main interface.

Detection results.

Quantify the time course.

Version 0.5

• New user interface
• Support one or two images
• Support one or two channels
• Select which channel is background and which is signal
• Support linear fitting
• Limit the range of τ and ‘a’ in curve fitting

Version 0.42

• Option to choose radius range (in pixels)
• For each bead center, choose a circle with the highest mean value in channel 2. Extract the curve based on pixels in this curve as well as one circle outside it and one circle inside it.
• Bug fix: index for the control beads labeled in the output

Version 0.41

• Bug fix: using unaligned background as F0

Version 0.4

• Select-control beads after detection
• Report curves, ΔF and τ for control beads
• Save to Excel support platforms other than Windows
• Include summary information in the Excel file
• More output figure types
• Bug fixes; Now tested on Matlab 2010b for better compatibility

Version 0.3

• Mean delta F from the entire image (blue channel)
• DeltaF and tau of the selected beads (blue channel)
• Coordinates of location for selected beads
• Time-lapse (in the blue channel) with the selected beads circled
• Set Delta F/F or simply Delta F

Version 0.23

• Support LSM and TIFF
• Can choose a time range in the GUI
• Algorithms updated to allow threshold setting for faster bead detection
• Set thresholds to accelerate the program for very dense image
• All the images are saved to the output folder as tiff format