error while running env_setup.sh script: about metagem HOT 8 CLOSED

I tried to solve it by adding

export -f conda
export -f __conda_activate
export -f __conda_reactivate
export -f __conda_hashr

to my bashrc file

This allowed conda to continue and install mamba. However, when the script enters the next step and tries to download and set up the metaGEM conda environment, I get:

bash: line 1: mamba: command not found

from metagem.

Hi Sam,

Thanks for reporting the bug, I have not encountered this problem before. Are you setting up metaGEM on the cluster or on your local machine?

From what you are telling me it sounds like the script is having trouble finding your mamba installation. Can you verify that there is indeed a mamba conda env installed on your system by running conda --info envs? If there is an environment called mamba, can you then run source activate mamba && mamba --version? If mamba is properly installed you should get an output like:

mamba 0.10.0
conda 4.10.1

from metagem.

Hi Francisco,

Thank you for your reply. I'm trying to set up metaGEM on a local machine with 40 threads and 500 GB RAM.

There is indeed a mamba conda env now, and when I run source activate mamba && mamba --version I get:

mamba 0.13.0
conda 4.10.1

I don't think it is the first time conda gets called from a bash script, I think that has happened before when installing MG-atlas, so I'm not sure what is causing this. Maybe I should try the manual setup?

from metagem.

Hey Sam,

I am curious, what happens if you activate the mamba env and then run bash env_setup.sh?

In any case, if you want to skip the script and set up manually then could you please try activating the mamba env, moving into your metaGEM folder, and running:

mamba env create --quiet --prefix ./envs/metagem -f envs/metaGEM_env.yml

This should set up a conda environment and install most of the core packages.

Also, just in case you haven't already seen it, I recommend you check out the google colab notebook. In section 2) you can see how to finish manually setting up metaGEM.

Please let me know if you have any further issues with the env_setup.sh script or with manual setup.

Best wishes,
Francisco

from metagem.

Hi Francisco,

Ok, I did not yet see the google colab notebook. Very usefull, thanks! In the meantime I started following the Manual setup, but by doing so, I now realize the metagem, metawrap and prokkaroary environments are located in:

/home/slambrecht/miniconda2/envs/

and not in metaGEM/envs/

I assume I can not just copy the env directories to metaGEM/envs/. Would it be best to remove them, and start anew, following the advice you gave me above?

Kind regards,
Sam

from metagem.

Hey Sam,

If you are confident that those envs are functional and properly installed, then you can modify your config.yaml file like so:

1. Open your config.yaml file and go to the envs section at the bottom:
   

   metaGEM/config.yaml

   Lines 79 to 82 in 98facf2

   	envs:
   	metagem: envs/metagem
   	metawrap: envs/metawrap
   	prokkaroary: envs/prokkaroary

2. Append path prefix to the envs, in your case:

envs:
    metagem: /home/slambrecht/miniconda2/envs/metagem
    metawrap: /home/slambrecht/miniconda2/envs/metawrap
    prokkaroary: /home/slambrecht/miniconda2/envs/prokkaroary

Now snakemake should be able to identify and activate envs within jobs.

If you think your envs may have been corrupted/incompletely installed then you can simply remove them and re-install them up in your envs folder.

conda env remove -n ENV_NAME

Let me know if this works out for you.

Best,
Francisco

from metagem.

Hi Francisco,

Great, thank you! Regarding CPLEX, you write

install this dependency manually within the metagem conda environment.

Does that mean I have to specify /home/slambrecht/miniconda2/envs/metagem/bin, when InstallAnywhere prompt asks me where I would like to install CPLEX Optimization Studio 20.1.0.?

Or how should I install CPLEX manually within the metagem conda environment?

from metagem.

Hi Sam,

Thanks for pointing that out, I should clarify: I mean activate the metagem env and then run the installer. I believe you can install CPLEX anywhere, but it does not hurt to put it in that path. Let me know if you have trouble setting up CPLEX.

from metagem.