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Baizhou12138 avatar Baizhou12138 commented on July 29, 2024

I'm wondering if I can use my existing annotated fasta file to directly perform the "Reconstruct & evaluate genome-scale metabolic models with CarveMe and memote" and "Species metabolic coupling analysis with SMETANA" in the workflow. Please let me know if my question is not clear enough. i'm really looking forward to your help. thanks again.

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franciscozorrilla avatar franciscozorrilla commented on July 29, 2024

Hey Baizhou, thanks for posting your issue with screenshots and explanations. I believe that this is not a bug, rather you are providing CarveMe with the incorrect input file. You need to translate the DNA to protein and perform open reading frame (ORF) annotation. For reference have a look at the following tutorial https://github.com/franciscozorrilla/EMBOMicroCom/blob/main/exercises/exercise_4.md

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