Analysis of RNA sequencing of alpha synuclein treated undifferentiated THP-1 monocytic cells.
- 5x105 cells/well in 6 well dish. 2ml of media -> 2.5x105 cells/ml
- 24-hr treatment
- Treated with WT monomeric (ASM) or preformed fibrillar (PFF) alpha synuclein
- ASM 10μg/ml
- PFF 1 and 10μg/ml
- RNA collected in 1ml TRIzol
- TURBO DNase treated
- Cold ethanol precipitated and suspended in 35μl nuclease-free H2O
- polyA selection cDNA library prep
- 2x150bp Illumina Hi-Seq performed by GENEWIZ