We did some things! Now we're on to summer2021 but going to still use this repo
Central dogma of molecular biology intro, Biological sequence information, General transfers of biological sequential information, Special transfers of biological sequential information (key to RNA-seq), Transfers of information not explicitly covered in the theory (especially Post-translational modification, methylation),
DNA sequencing background intro, applications, Illumina (Solexa) sequencing, sample preparation, computational challenges, ethical issues
RNA-seq background Intro, library prep, transcriptome assembly - guided, gene expression quantification, differential expression, applicatkons to medicine, large-scale sequencing efforts
- extra: fusion gene detection
brainstormed list of things we could work on
- figure out how to load formatted heatmaps into Morpheus, write script
- include clustered heatmaps so we can run clustering externally
- build heatmaps from arbitrary set of experiments
- probably needs to have differential metadata generated
- maybe add multiple layers i.e. logFc and adj.P.Value
- "fix" interactive Venn diagram tool
- script for geneBody coverage - make this part of the pipeline
- new GSEA script - use use command line GSEA? investigate other options?
- run genewalk analysis on some sample data, build script to do so consistently