cidarlab / fpselection Goto Github PK
View Code? Open in Web Editor NEWAlgorithms for the selection of fluorescent reporters.
Home Page: http://fpselection.org/
License: BSD 3-Clause "New" or "Revised" License
Algorithms for the selection of fluorescent reporters.
Home Page: http://fpselection.org/
License: BSD 3-Clause "New" or "Revised" License
Should be in this format:
FP; Laser; Detector
Remove all options for algorithms and make the default algorithm: Simulated Annealing.
Each Fluorophore, Laser, Filter should have its own plot.
Have the following input fields:
Cytometer Settings - Required
Fluorophore Spectra List - Required
Brightness - Required
Autofluoresence - Optional
N - Required
Plots Info should have the following format:
FP ; Laser ; Filter ; Signal ; Bleedthrough
To more accurately portray bleedthrough information I updated the method in ProteinSelector
that generates the noise treemap. I noticed there was a lot of references to a noiseKey in DataAnalytics, so you should double check those calculations if you need to.
Bleedthrough for a given FP & Detector is now represented as the sum of expressions of all other FPs using the given FP's selected laser. (Before it was of all FPs intended to be used with other lasers, not all other FPs)
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