cancerit / allelecount Goto Github PK

Support code for NGS copy number algorithms. Takes a file of locations and a [cr|b]am file and generates a count of coverage of each allele [ACGT] at that location (given any filter settings)

Home Page: http://cancerit.github.io/alleleCount/

License: GNU Affero General Public License v3.0

Makefile 2.92% C 56.16% Shell 9.40% Perl 29.00% Dockerfile 2.52%

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wisekh6 xtmgah danchubb bhernando wook2014 davidstreid abcoxyzide iovlaicu

allelecount's Issues

Installation error

Dear Keiran and David
I'm having the following problem when running the setup script. Could you please help me?
Many thanks, ke

-n Building alleleCounter ...
rm -f ./src/.o *~ ./bin/alleleCounter ./bin/ ./tests/tests_log ./tests/bam_access_tests ./src/.gcda ./src/.gcov ./src/*.gcno .gcda .gcov .gcno ./tests/.gcda ./tests/.gcov ./tests/.gcno
rm: ./bin/alleleCounter.dSYM: is a directory
make: *** [clean] Error 1

Throw informative error when BAM index missing

Currently alleleCounter throws a trace when it is run on a BAM file that has not been indexed. The trace does not mention the missing index, which would be a helpful message.

Example trace:

[ERROR] (src/bam_access.c: bam_access_openhts:55 errno: No such file or directory) HTS index file temp.bam failed to open.
[ERROR] (./src/alleleCounter.c: main:255 errno: None) Error trying to open sequence/index files 'temp.bam'.
*** glibc detected *** alleleCounter: free(): invalid pointer: 0x0000003b6b352a38 ***
======= Backtrace: =========
/lib64/libc.so.6[0x3b6b0722ef]
/lib64/libc.so.6(cfree+0x4b)[0x3b6b07273b]
alleleCounter(hfile_destroy+0x2e)[0x44e5be]
alleleCounter(hclose+0x34)[0x44e664]
alleleCounter(bgzf_close+0x6c)[0x44889c]
alleleCounter(hts_close+0x3d)[0x40a84d]
alleleCounter(bam_access_closehts+0x34)[0x407d14]
alleleCounter(main+0xde)[0x45514e]
/lib64/libc.so.6(__libc_start_main+0xf4)[0x3b6b01d994]
alleleCounter[0x407b89]
======= Memory map: ========
00400000-00461000 r-xp 00000000 00:bd 4069614364                         /home/sdentro/software/bin/alleleCounter
00660000-00661000 rw-p 00060000 00:bd 4069614364                         /home/sdentro/software/bin/alleleCounter
1a8fd000-1a926000 rw-p 1a8fd000 00:00 0                                  [heap]
3629000000-362900d000 r-xp 00000000 08:01 3040234                        /lib64/libgcc_s-4.1.2-20080825.so.1
362900d000-362920d000 ---p 0000d000 08:01 3040234                        /lib64/libgcc_s-4.1.2-20080825.so.1
362920d000-362920e000 rw-p 0000d000 08:01 3040234                        /lib64/libgcc_s-4.1.2-20080825.so.1
3b6ac00000-3b6ac1c000 r-xp 00000000 08:01 3039897                        /lib64/ld-2.5.so
3b6ae1b000-3b6ae1c000 r--p 0001b000 08:01 3039897                        /lib64/ld-2.5.so
3b6ae1c000-3b6ae1d000 rw-p 0001c000 08:01 3039897                        /lib64/ld-2.5.so
3b6b000000-3b6b14d000 r-xp 00000000 08:01 3040106                        /lib64/libc-2.5.so
3b6b14d000-3b6b34d000 ---p 0014d000 08:01 3040106                        /lib64/libc-2.5.so
3b6b34d000-3b6b351000 r--p 0014d000 08:01 3040106                        /lib64/libc-2.5.so
3b6b351000-3b6b352000 rw-p 00151000 08:01 3040106                        /lib64/libc-2.5.so
3b6b352000-3b6b357000 rw-p 3b6b352000 00:00 0 
3b6b800000-3b6b816000 r-xp 00000000 08:01 3040117                        /lib64/libpthread-2.5.so
3b6b816000-3b6ba15000 ---p 00016000 08:01 3040117                        /lib64/libpthread-2.5.so
3b6ba15000-3b6ba16000 r--p 00015000 08:01 3040117                        /lib64/libpthread-2.5.so
3b6ba16000-3b6ba17000 rw-p 00016000 08:01 3040117                        /lib64/libpthread-2.5.so
3b6ba17000-3b6ba1b000 rw-p 3b6ba17000 00:00 0 
3b6c800000-3b6c814000 r-xp 00000000 08:01 2499963                        /usr/lib64/libz.so.1.2.3
3b6c814000-3b6ca13000 ---p 00014000 08:01 2499963                        /usr/lib64/libz.so.1.2.3
3b6ca13000-3b6ca14000 rw-p 00013000 08:01 2499963                        /usr/lib64/libz.so.1.2.3
2b70dfa83000-2b70dfa85000 rw-p 2b70dfa83000 00:00 0 
2b70dfa96000-2b70dfb98000 rw-p 2b70dfa96000 00:00 0 
7fff87887000-7fff8789c000 rw-p 7ffffffe9000 00:00 0                      [stack]
7fff878d6000-7fff878d9000 r-xp 7fff878d6000 00:00 0                      [vdso]
ffffffffff600000-ffffffffffe00000 ---p 00000000 00:00 0                  [vsyscall]
Aborted

allelecount runs endlessly or gets killed on CentOS 7

allelecount runs endlessly without printing anything to results file or gets killed when using a large loci file. Even when allocating huge amounts of resources (16cpu, 90GB RAM). On centOS 7--works when I downsample the loci file down to a toy number of lines so I know none of the files are misformatted. Is there a max size for the loci file?

Option to run through file rather than use pileup/fetch

If flag selected at commandline would iterate through the file rather than pileup.
This would require a sorted input file check and a new method for iterating through the bam file.

Would give a large speed increase for input loci files with many concurrent or close clusters of positions.

patch not required

Updating to htslib 1.2.1+ removes the need for patching

Running aC on scRNA BAMs (10X or otherwise)

Hi there,
Is is essential for the HTS file to be paired-end for aC to work?
Specifically, aC only gives 0 counts (and 0 depth) when supplied with an scRNA BAM file.
Is there any way around this?
Thanks

Scipt convertAlleleCounts.r deleted?

Hi!

I have noticed that the script for convertAlleleCounts.r is not found anymore. However, the step is still listed on the nextflow website under "outputs docs".
Is there a reason it was deleted? Is it still available somewhere?

Running alleleCounter with or without reference file

Hi,

I may have missed this, but providing "reference file" (-r flag) is marked as optional in the help section.

Are there any advantages/disadvantages of providing reference file to alleleCounter?

Thanks.

Update to htslib / add CRAM support

Hi @DrJDev, there's some interest in this, is it a big job?

Memory use vs. size of SNP list

RAM usage spikes when I try a 200+ MB loci list.
Do you have any estimates for how much RAM is necessary for a given number of SNVs in the list?

RNA STAR aligned files may have different flags

STAR aligned files can have different bam flags set. Note about these in documentation and allelecount usage to help users understand why there's a lack of data.

10X test data doesn't work

$ git clone [email protected]:cancerit/alleleCount.git
...
$ cd alleleCount
$ ./setup.sh wibble
...
$ wibble/bin/alleleCounter -x -b testData/test10X.bam -l testData/test10X.loci -o example.tsv
Reading locis
Done reading locis
Using 10X processing mode.
kr2@kr2-test:alleleCount$ cat example.tsv 
#CHR	POS	Barcode	Count_A	Count_C	Count_G	Count_T	Good_depth

Confirmed test BAM covers relevant location:

$ cat testData/test10X.loci 
1	198661939
$ samtools view -c testData/test10X.bam 1:198661939-198661939 
11538

Not possible to emit version on cmd

No option to print the version number

"-b|--hts-file" isn't obvious means BAM/CRAM

Feed back from user.

Admittedly, knowing that the file type is something accepted by HTSlib is a bit niche.

migrate perl alleleCounter.pl to wrap the C version

The perl version has a few options that reformat the output. To reduce dependancies (e.g Bio::DB::HTS) we can change this to wrap the C version of alleleCounter and just have it do any relevant formatting.

Can I use this tools on RNAseq BAM file ?

I want to calculate the allele-based expression using ASCAT-V3.0 gave loci's file, Dose this tool suit for that?

Prototype overlapping read count corrections in alleleCounter.pl

Overlapping read from the same pair are becoming a common feature of modern NGS sequencing.

Prototype changes in the perl version to be compared against the eventual C one.

Any read that disagrees with it's mate at the same position should be counted for each allele and double counted for depth so no bias occurs.

e.g.

#Read of pair agree at same position
Read 1 = A
Read 2 = A
Results in: Allele_A = 1, depth = 1

#Read of pair DISagree at same position
Read 1 = A
Read 2 = T
Results in: Allele_A = 1, Allele_T = 1, depth = 2

Null Iterator Error

Hi,

I recently installed alleleCount v4.2.1 on an HPC cluster using gcc 7.4.0. The installation ran without any problems. However, when testing with example data I receive a long error message, see attached, but here is the top section:

Reading locis
Done reading locis
[E::sam_itr_next] Null iterator
[ERROR] (src/bam_access.c: bam_access_get_position_base_counts:454 errno: No such file or directory) Error detected (-2) when trying to iterate through region.
[ERROR] (./src/alleleCounter.c: main:448 errno: None) Error retrieving stats from bam file for position Y:2934912
*** Error in `alleleCounter': double free or corruption (out): 0x0000000000414b20 ***

The command run was:
alleleCounter -l gender.loci -b test.bam -o test.out

The input files gender.loci and test.bam are from this repository. Running the same command with v4.0.0 works without any errors.

Can you help troubleshoot the problem? Thanks.

installation error

! Configure failed for Bio-DB-HTS-2.9. See /home/users/heskett/.cpanm/work/1515532118.89002/build.log for details. ! Installing the dependencies failed: Module 'Bio::DB::HTS' is not installed ! Bailing out the installation for alleleCount-v3.3.1.

linux 3.10.0-514.16.1.el7.x86_64

the build log says the same--> biodbhts is not installed. But isn't this included?

thanks!

base image update to Ubuntu 20.04

More maintenance for LTS versions in docker

htslib for dockerfile is not updated

alleleCount/Dockerfile

Line 6 in a074b3e

ENV VER_HTSLIB="1.7"

Split install method resulting in divergence of code, needs updating so setup.sh can call the build/* scripts like other repos to reduce the chances of this problem.

installation error on linux

OS: linux 3.10.0-514.16.1.el7.x86_64

Doesn't the install include perl dependencies?

`Max compilation CPUs set to 6
App::cpanminus is up to date. (1.7043)
Previously installed base perl deps...
already staged ...
Bio::DB::HTS already installed ...
Building htslib ... previously installed ...
samtools version is good (1.3.1)
Building alleleCounter ... previously installed ...
Installing Perl prerequisites ...--> Working on /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/perl/.
Configuring /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/perl ... OK
<== Installed dependencies for /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/perl/.. Finishing.
Installing alleleCount ...Writing Makefile for alleleCount
Writing MYMETA.yml and MYMETA.json
Skip blib/lib/Sanger/CGP/AlleleCount/PileupData.pm (unchanged)
Skip blib/lib/Sanger/CGP/AlleleCount/Genotype.pm (unchanged)
Skip blib/lib/Sanger/CGP/AlleleCount.pm (unchanged)
cp bin/alleleCounter.pl blib/script/alleleCounter.pl
/usr/bin/perl -MExtUtils::MY -e 'MY->fixin(shift)' -- blib/script/alleleCounter.pl
Manifying blib/man1/alleleCounter.pl.1
Manifying blib/man3/Sanger::CGP::AlleleCount::Genotype.3pm
PERL_DL_NONLAZY=1 /usr/bin/perl "-MExtUtils::Command::MM" "-e" "test_harness(0, 'blib/lib', 'blib/arch')" t/*.t
t/1_pm_compile.t .. ok
t/2_pl_compile.t ..
Dubious, test returned 2 (wstat 512, 0x200)
No subtests run
t/genotype.t ...... ok

Test Summary Report

t/2_pl_compile.t (Wstat: 512 Tests: 0 Failed: 0)
Non-zero exit status: 2
Parse errors: No plan found in TAP output
Files=3, Tests=36, 1 wallclock secs ( 0.04 usr 0.01 sys + 0.32 cusr 0.14 csys = 0.51 CPU)
Result: FAIL`

Can't locate autodie.pm in @INC (@INC contains: /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/perl/blib/lib /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/perl/blib/arch /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/alleleCount/lib/perl5/x86_64-linux-thread-multi /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/alleleCount/lib/perl5/x86_64-linux-thread-multi /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/alleleCount/lib/perl5 /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/alleleCount/lib/perl5/x86_64-linux-thread-multi /home/exacloud/lustre1/SpellmanLab/heskett/tools/alleleCount/alleleCount/lib/perl5 /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at t/2_pl_compile.t line 32. BEGIN failed--compilation aborted at t/2_pl_compile.t line 32. Failed 1/3 test programs. 0/36 subtests failed. make: *** [test_dynamic] Error 255

int(block_str) error

I am running allelecount on my bam files and WGS vcf file
for some files
I am getting this error

ValueError: invalid literal for int() with base 10: ''
block = int(block_str) - 1;

in which case, this would happen

"python /hpc/users/girdhk01/psychencode/files/bin/allelecounter/allelecounter.py --vcf $VCF
--sample $WGS_NAME${chr_no}
--bam $BAM_file
--ref $REF
--min_cov 2
--min_baseq 2
--min_mapq 2
--max_depth 100000
--o ${BAM_SAMPLE_NAME}_${chr_no}.ac.vcf"
done

Description

I would be helpful to have a description of what the software does and what it can be used for.

10x data

Hi,

I am using allelecount-4.0.0, and I test the module with 10X data:

alleleCounter \ 
    -l alleleCount-4.0.0/testData/test10X.loci \
    -b alleleCount-4.0.0/testData/test10X.bam \
    -o test \
    -m 20 \
    -r /home/BIOINFO_DATABASE/database_for_LongRanger/refdata-hg19-2.1.0/fasta/genome.fa.fai \
    -d

And the test file output:

#CHR    POS     Count_A Count_C Count_G Count_T Good_depth
1       198661939       0       0       0       0       0

Yeah, I use longranger hg19 reference with 'chr' prefix in contigs, but it works well in test.bam data.

So, is there any wrong for 10X mode?

Meanwhile, my data is wgs 10X data, which barcode tag information is different from your provided 10Xtest, is this okay?

Thanks

AlleleCounter for unpaired data

When I try to run alleleCounter for unpaired seq data i get 0's for all positions, though samtools depth gives me proper results (high coverage of selected positions). Sam header for the dataset im trying to analyse is in concordance with loci file.
I found that Genotype.pm module requires sam flag 2 (read paired). Though, if I change it to 0, soft still dont give me proper result.
So is alleleCounter intended for the use for unpaired data? Or there is another kind of problem with dataset im using?

test failed: t/01bam.t .... Failed 235/235 subtests

Hi,
I am trying to install alleleCount-3.3.1 on Ubuntu 16.04 but couldn't pass the test:
" t/01bam.t .... Failed 235/235 subtests"
I have the same issue when installing PCAP-core-4.0.5.
Would really appreciate if you can give me some advice here.
I have attached my screen output here:

Max compilation CPUs set to 6
App::cpanminus is up to date. (1.7043)
Previously installed base perl deps...
already staged ...
Building Bio::DB::HTS ...
ls
echo '/* Empty config.h generated by Makefile */' > config.h
echo '#define HTS_VERSION "1.3.2"' > version.h
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o kfunc.o kfunc.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o knetfile.o knetfile.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o kstring.o kstring.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o bgzf.o bgzf.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o faidx.o faidx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o hfile.o hfile.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o hfile_net.o hfile_net.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o hts.o hts.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o md5.o md5.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o regidx.o regidx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o sam.o sam.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o synced_bcf_reader.o synced_bcf_reader.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o vcf_sweep.o vcf_sweep.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o tbx.o tbx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o vcf.o vcf.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o vcfutils.o vcfutils.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_codecs.o cram/cram_codecs.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_decode.o cram/cram_decode.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_encode.o cram/cram_encode.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_external.o cram/cram_external.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_index.o cram/cram_index.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_io.o cram/cram_io.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_samtools.o cram/cram_samtools.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/cram_stats.o cram/cram_stats.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/files.o cram/files.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/mFILE.o cram/mFILE.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/open_trace_file.o cram/open_trace_file.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/pooled_alloc.o cram/pooled_alloc.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/rANS_static.o cram/rANS_static.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/sam_header.o cram/sam_header.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/string_alloc.o cram/string_alloc.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/thread_pool.o cram/thread_pool.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/vlen.o cram/vlen.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o cram/zfio.o cram/zfio.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o kfunc.pico kfunc.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o knetfile.pico knetfile.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o kstring.pico kstring.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o bgzf.pico bgzf.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o faidx.pico faidx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o hfile.pico hfile.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o hfile_net.pico hfile_net.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o hts.pico hts.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o md5.pico md5.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o regidx.pico regidx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o sam.pico sam.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o synced_bcf_reader.pico synced_bcf_reader.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o vcf_sweep.pico vcf_sweep.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o tbx.pico tbx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o vcf.pico vcf.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o vcfutils.pico vcfutils.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_codecs.pico cram/cram_codecs.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_decode.pico cram/cram_decode.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_encode.pico cram/cram_encode.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_external.pico cram/cram_external.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_index.pico cram/cram_index.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_io.pico cram/cram_io.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_samtools.pico cram/cram_samtools.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/cram_stats.pico cram/cram_stats.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/files.pico cram/files.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/mFILE.pico cram/mFILE.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/open_trace_file.pico cram/open_trace_file.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/pooled_alloc.pico cram/pooled_alloc.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/rANS_static.pico cram/rANS_static.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/sam_header.pico cram/sam_header.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/string_alloc.pico cram/string_alloc.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/thread_pool.pico cram/thread_pool.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/vlen.pico cram/vlen.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -fpic -c -o cram/zfio.pico cram/zfio.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o bgzip.o bgzip.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o htsfile.o htsfile.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o tabix.o tabix.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/fieldarith.o test/fieldarith.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/hfile.o test/hfile.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/sam.o test/sam.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/test-regidx.o test/test-regidx.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/test_view.o test/test_view.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/test-vcf-api.o test/test-vcf-api.c
gcc -g -Wall -O2 -fPIC -Wno-unused -Wno-unused-result -I. -c -o test/test-vcf-sweep.o test/test-vcf-sweep.c
ar -rc libhts.a kfunc.o knetfile.o kstring.o bgzf.o faidx.o hfile.o hfile_net.o hts.o md5.o regidx.o sam.o synced_bcf_reader.o vcf_sweep.o tbx.o vcf.o vcfutils.o cram/cram_codecs.o cram/cram_decode.o cram/cram_encode.o cram/cram_external.o cram/cram_index.o cram/cram_io.o cram/cram_samtools.o cram/cram_stats.o cram/files.o cram/mFILE.o cram/open_trace_file.o cram/pooled_alloc.o cram/rANS_static.o cram/sam_header.o cram/string_alloc.o cram/thread_pool.o cram/vlen.o cram/zfio.o
ranlib libhts.a
gcc -pthread -o bgzip bgzip.o libhts.a -lz
gcc -pthread -o htsfile htsfile.o libhts.a -lz
gcc -pthread -o tabix tabix.o libhts.a -lz
gcc -pthread -o test/fieldarith test/fieldarith.o libhts.a -lz
gcc -pthread -o test/hfile test/hfile.o libhts.a -lz
gcc -pthread -o test/sam test/sam.o libhts.a -lz
gcc -pthread -o test/test-regidx test/test-regidx.o libhts.a -lz
gcc -pthread -o test/test_view test/test_view.o libhts.a -lz
gcc -pthread -o test/test-vcf-api test/test-vcf-api.o libhts.a -lz
gcc -pthread -o test/test-vcf-sweep test/test-vcf-sweep.o libhts.a -lz
gcc -shared -Wl,-soname,libhts.so.1 -pthread -o libhts.so kfunc.pico knetfile.pico kstring.pico bgzf.pico faidx.pico hfile.pico hfile_net.pico hts.pico md5.pico regidx.pico sam.pico synced_bcf_reader.pico vcf_sweep.pico tbx.pico vcf.pico vcfutils.pico cram/cram_codecs.pico cram/cram_decode.pico cram/cram_encode.pico cram/cram_external.pico cram/cram_index.pico cram/cram_io.pico cram/cram_samtools.pico cram/cram_stats.pico cram/files.pico cram/mFILE.pico cram/open_trace_file.pico cram/pooled_alloc.pico cram/rANS_static.pico cram/sam_header.pico cram/string_alloc.pico cram/thread_pool.pico cram/vlen.pico cram/zfio.pico -lz -lm
ln -sf libhts.so libhts.so.1
Created MYMETA.yml and MYMETA.json
Creating new 'Build' script for 'Bio-DB-HTS' version '2.3'
x86_64-linux-gnu-gcc -I/home/keyuan/battenberg_related/alleleCount-3.3.1/install_tmp/bioDbHts/htslib/htslib -I/usr/lib/x86_64-linux-gnu/perl/5.22/CORE -DVERSION="2.3" -DXS_VERSION="2.3" -fPIC -D_IOLIB=2 -D_FILE_OFFSET_BITS=64 -Wno-error -Wformat=0 -Wno-unused-result -c -D_REENTRANT -D_GNU_SOURCE -DDEBIAN -fwrapv -fno-strict-aliasing -pipe -I/usr/local/include -D_LARGEFILE_SOURCE -D_FILE_OFFSET_BITS=64 -O2 -g -o lib/Bio/DB/HTS.o lib/Bio/DB/HTS.c
ExtUtils::Mkbootstrap::Mkbootstrap('blib/arch/auto/Bio/DB/HTS/HTS.bs')
x86_64-linux-gnu-gcc -shared -L/usr/local/lib -fstack-protector-strong -o blib/arch/auto/Bio/DB/HTS/HTS.so lib/Bio/DB/HTS.o -L/home/keyuan/battenberg_related/alleleCount-3.3.1/install_tmp/bioDbHts/htslib -Wl,-rpath,/home/keyuan/battenberg_related/alleleCount-3.3.1/install_tmp/bioDbHts/htslib -lhts -lpthread -lz
x86_64-linux-gnu-gcc -I/home/keyuan/battenberg_related/alleleCount-3.3.1/install_tmp/bioDbHts/htslib/htslib -I/usr/lib/x86_64-linux-gnu/perl/5.22/CORE -DVERSION="2.3" -DXS_VERSION="2.3" -fPIC -D_IOLIB=2 -D_FILE_OFFSET_BITS=64 -Wno-error -Wformat=0 -Wno-unused-result -c -D_REENTRANT -D_GNU_SOURCE -DDEBIAN -fwrapv -fno-strict-aliasing -pipe -I/usr/local/include -D_LARGEFILE_SOURCE -D_FILE_OFFSET_BITS=64 -O2 -g -o lib/Bio/DB/HTS/Faidx.o lib/Bio/DB/HTS/Faidx.c
ExtUtils::Mkbootstrap::Mkbootstrap('blib/arch/auto/Bio/DB/HTS/Faidx/Faidx.bs')
x86_64-linux-gnu-gcc -shared -L/usr/local/lib -fstack-protector-strong -o blib/arch/auto/Bio/DB/HTS/Faidx/Faidx.so lib/Bio/DB/HTS/Faidx.o -L/home/keyuan/battenberg_related/alleleCount-3.3.1/install_tmp/bioDbHts/htslib -Wl,-rpath,/home/keyuan/battenberg_related/alleleCount-3.3.1/install_tmp/bioDbHts/htslib -lhts -lpthread -lz
t/00_load.t .. ok
t/01bam.t .... Failed 235/235 subtests
t/02faidx.t .. ok
t/04tabix.t .. ok
t/05vcf.t .... ok
t/06kseq.t ... ok

Test Summary Report
t/01bam.t (Wstat: 139 Tests: 0 Failed: 0)
Non-zero wait status: 139
Parse errors: Bad plan. You planned 235 tests but ran 0.
Files=6, Tests=245, 0 wallclock secs ( 0.03 usr 0.01 sys + 0.53 cusr 0.04 csys = 0.61 CPU)
Result: FAIL
Failed 1/6 test programs. 0/245 subtests failed.

Many thanks!
Vivi

Pre-release tag for fragment analysis

Based on feature/overlapping_reads

Confirm #30, #32, #37 resolved in branch
Create pre-release tag on feature/overlapping_reads

AlleleCounter bahavior with soft-clipped reads

Hello CancerIT Team,

could you confirm if soft-clipped reads are taken into account in pile-up calculations?

Best wishes
Dominik

Clipping reads and reference bias

Hi,

We have noticed that the Illumina aligner Isaac soft clips the end of reads that have non-reference bases https://www.biorxiv.org/content/10.1101/836171v1. This affects battenberg results so to correct it we have forked a version of allelecounter that removes biased counts by clipping all reads by n bases (e.g. -e 5 end clips 5 bases) https://github.com/danchubb/alleleCount-FixVAF

The main change is in https://github.com/danchubb/alleleCount-FixVAF/blob/dev/c/src/bam_access.c line 131 where the count is skipped if a position is within e bases of the end.

It might be something you'd want to merge in or at least be aware of if you use allelecounter for isaac aligned data.

also, this seemed to be the easiest way of dealing with it in allele counter and I don't think it will break anything else but please let me know if you have a more elegant solution.

Thanks,

Dan

Add travis status to README

In next release or hotfix please

how to install in R

Hello,
can we install this package to be used in R ... and how ??
thank you in advance,
Best,
Esraa

Is LWP::UserAgent required?

Hi, I am trying to build this package from source with local-only dependencies and I would like to know, whether the Perl package LWP::UserAgent is required for this package to function properly. I see that you are installing it in the opt-build script, but it is not mentioned in the perl/Makefile.PL as a prerequisite.

Is htslib 1.10.2 supported?

The bioconda recipe has this pinned to htslib 1.9-- do you know if 1.10.2 works? I'm updating a bunch of recipes that are linked to old versions of htslib, and this is one of them.

Indel support

Is it possible that this tool will ever offer support for counting indels?

sorting of loci file for dense-snps

sort -k1,1 -n 2,2n loci_unsrt.tsv > losi_sorted.tsv does not work.
Did you mean:
sort -k1,1n -k 2,2n loci_unsrt.tsv > losi_sorted.tsv
or
sort -k1,1 -k2,2n loci_unsrt.tsv > losi_sorted.tsv

Either way, it would be better just to say if you want the chromosomes in alphabetical or numerical (natural) order.

Add sam flag filter option

Allow override of the SAM flag filter at commandline.

Confusing error when input is unmapped

This code isn't intended for unmapped BAM files, but logging how it appears.

When an alignment file is not mapped you will see an error similar to this:

[ERROR] (src/bam_access.c: bam_access_get_position_base_counts:454 errno: No such file or directory) Error detected (-2) when trying to iterate through region.
[ERROR] (./src/alleleCounter.c: main:448 errno: None) Error retrieving stats from bam file for position �:65885569

The error doesn't print the contig correctly for some reason at this line:

alleleCount/c/src/alleleCounter.c

Line 448 in b2158f0

    
           check(ret==0,"Error retrieving stats from bam file for position %s:%d",locis[j]->chr,locis[j]->pos);

Rather than this being confusing, this should be prevented by checking the header contains @SQ lines and giving an appropriate message if they are absent.

C alleleCounter - filter by long form

Using the long form of the -f and -F options results in a segfault.

I started to dig in to this and found that the options are not marked as taking values:

alleleCount/c/src/alleleCounter.c

Lines 91 to 92 in e16b76d

    
           {"required-flag", no_argument, 0, 'f'}, 
        
           {"filtered-flag", no_argument, 0, 'F'},

Should this be optional_argument?

With or without that change valgrid gives:

==5663== Memcheck, a memory error detector
==5663== Copyright (C) 2002-2011, and GNU GPL'd, by Julian Seward et al.
==5663== Using Valgrind-3.7.0 and LibVEX; rerun with -h for copyright info
==5663== Command: /.../GitHub/alleleCount/perl/bin/../../c/bin/alleleCounter --loci-file testData/loci_22.txt --hts-file testData/test.bam --output-file test --min-base-qual 30 --min-map-qual 35 --required-flag 3
==5663== 
==5663== Invalid read of size 1
==5663==    at 0x54A66E4: ____strtol_l_internal (strtol_l.c:298)
==5663==    by 0x461A42: alleleCounter_setup_options (stdlib.h:286)
==5663==    by 0x408E00: main (alleleCounter.c:366)
==5663==  Address 0x0 is not stack'd, malloc'd or (recently) free'd
==5663== 
==5663== 
==5663== Process terminating with default action of signal 11 (SIGSEGV)
==5663==  Access not within mapped region at address 0x0
==5663==    at 0x54A66E4: ____strtol_l_internal (strtol_l.c:298)
==5663==    by 0x461A42: alleleCounter_setup_options (stdlib.h:286)
==5663==    by 0x408E00: main (alleleCounter.c:366)

This occurs on both --required-flag 3 and --filtered-flag 3852 but not on the short forms -f 3 and -F 3852 for some reason.

Unpaired data - No alleleCount when reads available

Hi developers,

Thanks for developing this tool. I have questions when I use this tool. I found I cannot count any allele in a bam file, even with -m 0 -q 0. However, I found the bam file has nothing wrong. Below is an example.

Why this happen? Any suggestions?

Best,

Shixiang

$ alleleCounter -l test.loc -b ~/data/gdc/wes/TCGA-AA-3869-10A-01W-0995-10_hg19_Illumina_gdc_realn.bam -o test.txt -m 0 -q 0

Reading locis
Done reading locis


$ cat test.txt 
#CHR	POS	Count_A	Count_C	Count_G	Count_T	Good_depth
chr3	58125556	0	0	0	0	0
chr3	58125557	0	0	0	0	0
chr3	58125558	0	0	0	0	0
chr3	58125559	0	0	0	0	0
chr3	58125560	0	0	0	0	0


$ samtools view -b ~/data/gdc/wes/TCGA-AA-3869-10A-01W-0995-10_hg19_Illumina_gdc_realn.bam chr3:58125556-58125560 | samtools view -
[W::hts_idx_load3] The index file is older than the data file: /public/home/zhaoqi/data/gdc/wes/TCGA-AA-3869-10A-01W-0995-10_hg19_Illumina_gdc_realn.bai
HWI-ST115_0148:1:1:2828:131031#0	0	chr3	58125457	60	100M	*	0	0	TTTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGC	46659B=BA?B@DB?@?>B>@?DB?@EA<ACC@7ABA?C>AAAAABC@CAB@AC?@??@@AC@@DBCCCC@D?DC@A>?=>=ABCBA@=B?<>=@@;<;8	BD:Z:JJAKPPLCMLJKHLKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMNJJLMKAKJMLOOOLCMLLNN	MD:Z:100	RG:Z:0.1	BI:Z:NNHOQROHPPNOMQPQQPOOPQOPQPOQNOPNOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJKQQPIPORQRRQNGNPPQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:66:8947:69599#0	1024	chr3	58125457	60	100M	*	0	0	TTTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGC	46659@=AA>B@DB>@@>B?@?DB??D@A@CBA8@B?>C>@@B?@BB@CAC?@B@A?@A@?@<<C@BBBBBE@DBCB>@><>ABBB?A?B??DBA@9=:8	BD:Z:IIAJPOLBLMJKIMKLMKKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLJJNLLKLKJKMKMJAAAJKHIIAKLMMJJKLJAKJMMOPOKCLLLNN	MD:Z:100	RG:Z:0	BI:Z:MMGOQRPGPONNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLOGQPQPKKQQOHPNQQRRPNGNOPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:61:8962:119547#0	16	chr3	58125457	60	100M	*	0	0	TTTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGA	><<:>CCC?CA?B@CB?C?C@@BF?AD@AA<A8BCE<A?EACEBCCAABADABCA>CACABB@?BCCCCA@>?CCB>CAA@>ABCD@>BD@>A@?77844	BD:Z:BKLMLBMMKLJNLLLMLLLNNMLMMMJMMJJMKMLMNMKJMLKJ@JMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKMNNLBMOOMLJJ	MD:Z:99C0	RG:Z:0.1	BI:Z:HOPOMGOQQOORQQQQQQQOQQQPQPOQQPOQOQQPRQPOQQPOHNQPOQPONPPPONOOOPQQPHHHOQLMOGNPOOJJNPOHNMPPQPQOHPQRQPNN	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:5:15563:60243#0	0	chr3	58125458	60	100M	*	0	0	TTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCG	3533=?@A>A?DB?@?>B>@?DB?=D@A@CBA7@A??C>@@B?@BB@CAC?AB@@?@A@@CB@DBBCBBBDADBBC>@>>>ABBB?@????CBB@<=:92	BD:Z:IIJLPLDLMKKIMLLMKKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIJNLMJLKJKMKMJAAAJKHIIAKLMMJJKLJAKIMMOOOKCMMLNNK	MD:Z:100	RG:Z:0	BI:Z:MMOORPIPONNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNHPPQPKKQQOHPNQQQRQOGPMPPPM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:22:21121:84028#0	0	chr3	58125459	60	100M	*	0	0	TCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGT	3329>??=B?DB??@?B>@?EA@?C>A@CC@7>BA?C>@AB?ACC>C?D@AC@A??A?@BB@DBCCCC@D@BBCB?A>>>A@CC@A?BA?B5@:.@=92;	BD:Z:JJMMMENMKKIMKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIJLMKAKJMLNNOKCNMMNNLM	MD:Z:100	RG:Z:0.1	BI:Z:NNOQPIQPNOMQPQQQPOPQOPPPPPNPOMOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPQPIPORQRRQOIPONQQNQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:24:18332:73108#0	1024	chr3	58125459	60	100M	*	0	0	TCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGT	4548>>@=A@DB>@@>B>@?DB??D@A@CBA7@B??C>@@B?@CB@CAC?BBAA?@A@ACB@EBCCCCBE@ECCB@B>>>BBCCA@?C@@ECCB>A<92;	BD:Z:JJMMMENMKKIMKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIJLMKAKJMLNNOKCNMMNNLM	MD:Z:100	RG:Z:0.1	BI:Z:NNOQPIQPNOMQPQQQPOPQOPPPPPNPOMOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPQPIPORQRRQOIPONQQNQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:67:17292:45690#0	1024	chr3	58125459	60	100M	*	0	0	TCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGT	332:<??=>=EB?A@>????EB?@CAAADC@7?B??C>???AACA@C@D?AAAA??A?ACB@EBCCCC?C@CABB?????<:BB@A?@@?DCCA>@>92;	BD:Z:JJMMMENMKKIMKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIJLMKAKJMLNNOKCNMMNNLM	MD:Z:100	RG:Z:0.1	BI:Z:NNOQPIQPNOMQPQQQPOPQOPPPPPNPOMOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPQPIPORQRRQOIPONQQNQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:22:13156:141937#0	0	chr3	58125460	60	100M	*	0	0	CCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTT	3388=><A?DA>>?>A>?>DA@?D>A@CC@7ABA>C?@ABAAAC@A@C>AB@@??B?ACB@DBC@CC@E@DCAB???5<@BCC@A?BA?B@BB?B>;0<=	BD:Z:JJMJEONKLIMLMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIILMKAKJMLNNNKCMNMONLMK	MD:Z:100	RG:Z:0.1	BI:Z:NNQNJQQNOMQPQQQPPPQOPPPOQNOPMNOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPPIPORQRRQOIQPOOQNQN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:1:15839:10992#0	1024	chr3	58125460	60	100M	*	0	0	CCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAGGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTT	4488;><A?DB>@@>B>@?DB??D@@@CBA7@B??C>@@B?@BB@CAC?@@>@>@B@@CB@DABBBCADAECBC>A>>>ABBB?@>B@?DBBB>A=:29;	BD:Z:IILIENOKLJMLMNKKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLKLKLMKLJJKMKMJAAAJKHIIAKLMMJJKLJAKILLOONJCMNNONKLJ	MD:Z:50A49	RG:Z:0	BI:Z:MMPNIRQNNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPOOOPPOOONPPPPNGGGOPLLNGPPPPKKQQOHPNQQQQPPIQOPNPMOM	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:44:12431:82847#0	0	chr3	58125461	60	100M	*	0	0	CAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTT	4756;:@?CB>@@>B>@?DB??D@@@CBA7AB??C>@@B?@BB@CAC?@B@@>@@@ABB@DBBBBBBD@DBCC?@?>>ABBB?@>B?@DBBB>B>=29<=	BD:Z:IIIAOOMLJNLMNLKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKMKLKIKMKMJAAAJKHIIAKLMMJJKLJAKILLNONJBMNNPOKLJA	MD:Z:100	RG:Z:0	BI:Z:MMNGRQPNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOQOKKQQOHPNQQQQPOIRPPPNMOMG	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:66:3322:117270#0	0	chr3	58125462	60	100M	*	0	0	AAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTC	36667>>CA?@?>B>@>DB??D@@@CBA7ABA?C>@@B?@CC@CAC?AC@A?@A@BCB@DBCCCC@DADBCC??>>>ABCCAA?B@@BCCB?BA?6==>=	BD:Z:JJBLOMMJNLNNLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLKAKJMLNNNJBMMMPOMMKAK	MD:Z:100	RG:Z:0.1	BI:Z:NNHPROPMQPQQQPPQROPPPOPNPOMONPPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHPORQRRQOIQQQQPLQNHO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:43:4574:152452#0	0	chr3	58125463	60	100M	*	0	0	TATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT	4252;<BA>@?>B>@>DB??A@A@CB@7@BA?C>@@B?@BB@CAC?@C@A?@AAACC@DACCCB@EAECCC?A=>>ABDC@@?B@@FCCB?BA?7?>=<>	BD:Z:JJLLMNKNMNNMKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJAKJMLNNNJBLMMOPMNKAKK	MD:Z:0A99	RG:Z:0.1	BI:Z:NNPPPPNQPQQQPPQRPPPPOPNOPMNOPOQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHOORQRRQOIQQQRQMONHOO	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:64:16055:12934#0	1024	chr3	58125463	60	100M	*	0	0	TATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT	4262;<BA=@@>B>@?DB??E@@@CBA7AB??C>@@B?@CB@CAC?AB@@??A?ABB@DBCCCC@EADCCC?A???@CCC@@?C@@ECCC?C@?7?>><?	BD:Z:JJLLMNKNMNNMKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJAKJMLNNNJBLMMOPMNKAKK	MD:Z:0A99	RG:Z:0.1	BI:Z:NNPPPPNQPQQQPPQRPPPPOPNOPMNOPOQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHOORQRRQOIQQQRQMONHOO	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:64:5368:84525#0	1024	chr3	58125463	60	100M	*	0	0	AATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT	4754=<B@>?@>A>@>DBA?E@B@CCA7AB??C??AB@ACC@C@CAAC?A?@@?ACB?CBCCCC@D@DCBB???=?ABCC@A?BA?DBBB?B@=7@?=;>	BD:Z:JJLLMNKNMNNMKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJAKJMLNNNJBLMMOPMNKAKK	MD:Z:100	RG:Z:0.1	BI:Z:NNPPPPNQPQQQPPQRPPPPOPNOPMNOPOQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHOORQRRQOIQQQRQMONHOO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:3676:113118#0	1024	chr3	58125463	60	100M	*	0	0	AATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT	3554<;@?=>=>=:=9>679A:=9=76'8.?<6;<;7.37A:A??AAA?@>?:@BA=>B@@@8=?D@ABB==<9:>AA=B;??;?=ABA>>@??4>=:99	BD:Z:IIKLNNLNMNNLLLLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJLKJKLKMJAAAJKHIIAKLMMJJKLJAKILLNNMJBLMNPPMMJAJJ	MD:Z:100	RG:Z:0	BI:Z:MMPOPPNPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOKJQQOHPNQQQQPOHQQRQPMMMGON	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:22:7204:119714#0	0	chr3	58125464	60	100M	*	0	0	ATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTG	46269@@>?@>B>@?BB??D@@@CBA8@A??C?@@B@@BB@@AC?AB@@>?A??BB>C?B@BB@C@EBBC?A??>ABBB?@>B??CBCB?B??7?@=<<:	BD:Z:IILJOLPMNOLLLMNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKKJKMJMJAAAJKHIIAKLMMJJKLJAKILLNNMIBLMMPPMNKAJJM	MD:Z:100	RG:Z:0	BI:Z:MMONPNROPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJKPQOHPNQQQQPOHQPRRQMOKGONP	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:27:5413:149006#0	1024	chr3	58125464	60	100M	*	0	0	TTAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTG	4526:@@>??>B???BA?:D@@?CBA8AB@?C=?@B?@BB@CAC?BB?@>@A@ABB@DB@BB@@C@DBBB?@?>>ABBB?@>AA?CBBB?B??7@@=:<8	BD:Z:IIJJOLPMNOLLLMNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKKJKMJMJAAAJKHIIAKLMMJJKLJAKILLNNMIBLMMPPMNKAJJM	MD:Z:0A99	RG:Z:0	BI:Z:MMPNPNROPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJKPQOHPNQQQQPOHQPRRQMOKGONP	NM:i:1	AS:i:99	XS:i:20
HWI-ST142_0240:1:3:16511:17168#0	1024	chr3	58125464	60	100M	*	0	0	ATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAACTAGGGGGTTTGTATGCACATATGCGGTTCTG	20396418;<=A>>=C?@>C>@>@@?79A>6@;=:>@>?>>?>A>BB>?9:2>??68B7?BBB?D%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:IILJOLPMNOLLLMNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKKJKMJMJAAAJKHIIKLLMMJJJLJAKILLNNMHJNMMPPMNMJJJM	MD:Z:68A6A10A7T5	RG:Z:0	BI:Z:MMONPNROPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNNPPPOJKJPNHPNQQQQPMMQPRRQMMMMONP	NM:i:4	AS:i:80	XS:i:20
HWI-ST142_0240:1:26:3068:140930#0	0	chr3	58125465	60	100M	*	0	0	TAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGT	4264>><?>>A?@?DB??D@@>CBA7@A??C?@@B@@BB@CAC?@B@@>@A@@BB@DBBBBBBD@DCBB?@>>?BBBB@@>B??CCBB?B??7@A@<=:;	BD:Z:IIJKMPONOMLLMOLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJJKMKLJAAAJKHIIAKLMMJJKLJAKILLNNMIALMMOPMNLBJJMI	MD:Z:100	RG:Z:0	BI:Z:MMNNNRQPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJQPOHPNQQQQPOHQPQRRNOMEONPM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:65:3969:160251#0	1024	chr3	58125465	60	100M	*	0	0	TAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGT	4264=><??>B>@=D??>C@@@CBA7@>??C>@@B?@BB@CAC?AB@?>@A?@BA@D@BBBB@D@DBBB?A???AABB?@>B@?DBBC>B?>7@A@<<:;	BD:Z:IIJKMPONOMLLMOLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJJKMKLJAAAJKHIIAKLMMJJKLJAKILLNNMIALMMOPMNLBJJMI	MD:Z:100	RG:Z:0	BI:Z:MMNNNRQPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJQPOHPNQQQQPOHQPQRRNOMEONPM	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:21:18981:131709#0	0	chr3	58125466	60	100M	*	0	0	AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT	3649<<?>>B>?>DB?@E>AADAA7AB@?C?@ABAACCAC@D?AA@@??A?BCB@EBC@CC@B@DCBB??>>=@BCD@A?BA?ECBB?B@?5ACC?@<<;	BD:Z:JJKIPOPONLLNNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JIMLNNNJBLLLNOMNMBLKNJK	MD:Z:100	RG:Z:0.1	BI:Z:NNOMSQRQQPPQRPQQQOPNOOMNOPOQPNPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPRRQOIQQQRRORNGMOPNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:22:5666:176436#0	1024	chr3	58125466	60	100M	*	0	0	AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT	373;<;<>=A<><A??<B?>@BA@7?>>>A;?AA@ACC@A@C@;A@A?@@=@???DBB?B@>B?BCC@?>=>><@>9:88@A?DB@B??@>6=@@>?:<;	BD:Z:JJKIPOPONLLNNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JIMLNNNJBLLLNOMNMBLKNJK	MD:Z:100	RG:Z:0.1	BI:Z:NNOMSQRQQPPQRPQQQOPNOOMNOPOQPNPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPRRQOIQQQRRORNGMOPNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:5:17906:70621#0	1024	chr3	58125466	60	100M	*	0	0	AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT	373:<;=>>@>?>EB@>C>@@CC@7ABA?C?@ABAACC@C@C@BC?A?@=;=>?;DBCBCC@D@ACCB????>ABCCA@?B@?DCC@?BA?7@CC??<;=	BD:Z:JJKIPOPONLLNNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JIMLNNNJBLLLNOMNMBLKNJK	MD:Z:100	RG:Z:0.1	BI:Z:NNOMSQRQQPPQRPQQQOPNOOMNOPOQPNPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPRRQOIQQQRRORNGMOPNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:62:3161:55853#0	1024	chr3	58125466	60	100M	*	0	0	AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT	4648;:>?=B>@?DB??D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@@@BBA@DBBBBBAD@DCBB>A>>?AABB?@?B??CABB?B=?8@BA?>:;=	BD:Z:IIKIQOPOMMLMOMLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIKMKMIAAAJKHIIAKLMMJJKLJAKILLNNMIAKMMOOMNLCKJMIJ	MD:Z:100	RG:Z:0	BI:Z:MMNLRQRQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPQNHPNQQQQPOHQPQQROPMGMNPMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:66:7395:110588#0	0	chr3	58125467	60	100M	*	0	0	ACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTT	44867<>=A>@?DB??D@@@CBA7@B??C>@AB?@BB@CAC?@B@@?@@@ABB@DBBBBBADADBBC>@?>?BBBC?@>B;?DBBB>C@?7@BB@A:;<=	BD:Z:IIIMPPQMMMMOMMMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJMKMJ@AAJKHIIAKLMMJJKLJAKILLNNMIAKLMOOLNLCLKMIJA	MD:Z:100	RG:Z:0	BI:Z:MMLPQRSPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPOGPNQQQQPOHQPQQQOQNGOLPMMG	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:63:9558:92531#0	16	chr3	58125467	60	100M	*	0	0	ACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTT	;:<9A?>B>B??CD??CA@@B@7@CD@@?DBBDBBB@A@@DABC@>CACABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@AA><=4654	BD:Z:INLLMNNNNNOONMNMJMNJJMKMMMOMLJMMKJAJNLKNKJHLLLJIIIIKLMJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKMMMKLBKNQMKAII	MD:Z:100	RG:Z:0	BI:Z:MPPPNOOQRPQQQPQQOQRPOQOQPPQPPNPPPOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNQRNNGMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:45:1957:27774#0	0	chr3	58125468	60	100M	*	0	0	TATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG	3255:<<<=:3>>@8@>>?BC:4A;:4:.:@AAACC>>?AABC?=;?A?ABB?DBCCCB@D@E@CB?>?9>AB@CAA?@A?BCCB?B?0/??B<@@=;<;	BD:Z:JJLLQQOMMNONLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKNNNJBLLLNNLNLBMLOJKAK	MD:Z:0C99	RG:Z:0.1	BI:Z:NNPPSRRPPQRPQQQPQNOOMNNPPPPOOPOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQQOIQQQRROROIONNNNHO	NM:i:1	AS:i:99	XS:i:20
HWI-ST115_0148:1:4:6609:9121#0	1024	chr3	58125468	60	100M	*	0	0	CATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG	48629<<A>@?DB??E@@@CBA7@B??C>A@B?@BC@CAC?AB@A?@A@ACB@EBCCCC@EAECCC?A??>@BCC@@?C@@ECCC?C@@8ACCAC>==><	BD:Z:JJMLQQOMMNONLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKNNNJBLLLNNLNLBMLOJKAK	MD:Z:100	RG:Z:0.1	BI:Z:NNQPSRRPPQRPQQQPQNOOMNNPPPPOOPOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQQOIQQQRROROIONNNNHO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:28:16780:10277#0	1024	chr3	58125468	60	100M	*	0	0	CAGAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG	48449<<A=@?DB??D@A@CBA7@B??C>@@B?@BB@CAC?@B@@?@A@ACB?DBBBBB@D@EABB?@>??AACB@@>B??BBAB?B@?7@BBAB=;<=:	BD:Z:IINKPQOMMNOMMNMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLKMJA@AJKHIIAKLMMJJKLJAKILLNNMIAKLLOOLMLCLLNIJAK	MD:Z:2T97	RG:Z:0	BI:Z:MMPOQSRPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNHONQQQQPOHQPQQQNQOHONNMMGO	NM:i:1	AS:i:97	XS:i:20
HWI-ST142_0240:1:8:13705:180053#0	1024	chr3	58125468	60	100M	*	0	0	CATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG	47629<<A=@?DB??D@@@CBA7@B??C>@@B?@BB@BAC?@B@@??B@AB@@DABBBB?D@DBBB?@>??BACB@@>B??DBBB>B@?7@BB@B=;=<:	BD:Z:IIMLQQOMMNOMMNMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLKMJA@AJKHIIAKLMMJJKLJAKILLNNMIAKLLOOLMLCLLNIJAK	MD:Z:100	RG:Z:0	BI:Z:MMPORSRPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNHONQQQQPOHQPQQQNQOHONNMMGO	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:45:17861:199730#0	0	chr3	58125469	60	100M	*	0	0	ATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGT	46249:@>??DA??DA@@CBA8@B??C?A@B?@CC@CAC?@B@@>ABA@CB@EBCCCCBEAECCB?@>>?BBCC@A?C@@ECCB?B@@8ACDAD???=:<	BD:Z:JJLNQPNMOONMMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMNNJBLLLNNLMLBLMOKKAKJ	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSRQPQRPQQQPQOOOMNNPOQPNPPNPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPOIQQQRROROIPOOLNHON	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:26:8574:182738#0	1024	chr3	58125469	60	100M	*	0	0	ATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGT	2438;:;<<<@A@:C===???6=A@;?<>=A?BCA=A@CABA@A=>?>@B>;:>@B@B>D>B@A@?@???:;@@@?>BA?>AC?>BA?7>?;??><9:57	BD:Z:IILMROOMNPMMNNLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJMJAA@JKHIIAKLMMJJKLJAKILLNNMIAKLLNOLMKCLLOJJAKI	MD:Z:100	RG:Z:0	BI:Z:MMOPSRROPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGPMQQQQPOHQPQQQNPOIPNPKMGOM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:46:21305:107554#0	1024	chr3	58125469	60	100M	*	0	0	ATANTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGT	222%9:;<<<DB?>D@@@CB@8AB??C?@@B?ABB>CAC?AA@@?@A@ABB@DBBBBB@D?CBBB??>>?BABB@A>B@>7ABB?B=@7@B@@B@>==27	BD:Z:IILIMLOMNPMMNNLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJMJAA@JKHIIAKLMMJJKLJAKILLNNMIAKLLNOLMKCLLOJJAKI	MD:Z:3G96	RG:Z:0	BI:Z:MMOMOOROPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGPMQQQQPOHQPQQQNPOIPNPKMGOM	NM:i:1	AS:i:98	XS:i:20
HWI-ST115_0148:1:42:17429:61202#0	0	chr3	58125470	60	100M	*	0	0	TAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT	42447><?>DB??D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@A?ACB@EBCCCCBDAECCC?A??>ABCCAA?C@@ECCB?C@@8ACCBD?@A>:<>	BD:Z:JJNNPONOPNMNMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMNJBLLLNNLMKBLLPKLAKJK	MD:Z:100	RG:Z:0.1	BI:Z:NNQQSQQQRPQQQPQOPOMNNPOPQNOQNOQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNIQQQRROROIPPPMLHONN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:64:7391:109414#0	1024	chr3	58125470	60	100M	*	0	0	TAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT	42447><?>DB??D@@@CBA7AB??C>@AB?@BC@CAC?AB@A>@A@ACB@EBCCCCBD@DCCC?@>>?@BCC@A?CA?ECCC?CA@7ACC@C?@A>:;>	BD:Z:JJNNPONOPNMNMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMNJBLLLNNLMKBLLPKLAKJK	MD:Z:100	RG:Z:0.1	BI:Z:NNQQSQQQRPQQQPQOPOMNNPOPQNOQNOQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNIQQQRROROIPPPMLHONN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:66:14210:184691#0	1024	chr3	58125470	60	100M	*	0	0	TAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT	32469><@>BA?@EA??BC@8:BA>C=?ABAACC@@@C@?A??>??>AA=@BAAACC@A@C@@B9@??=@BBC@B0=?;07/;4>A?7@BC=C@@??:<%	BD:Z:JJNNPONOPNMNMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMNJBLLLNNLMKBLLPKLAKJK	MD:Z:100	RG:Z:0.1	BI:Z:NNQQSQQQRPQQQPQOPOMNNPOPQNOQNOQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNIQQQRROROIPPPMLHONN	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:62:5505:107378#0	16	chr3	58125471	60	100M	*	0	0	AGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTG	>;9=<B??BD??DA@@BA7ABD?@>CBCEBCBAAA@CBBC@>CACABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@C?@>=4684	BD:Z:LLLLMOPPONONKMNJJMKMMMOMLJMMKJAJNLKNKJILLLIJJJJKLMJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJKAJKOJLDLMKIII	MD:Z:100	RG:Z:0	BI:Z:POOPNNPQRQQQOQRPOQOQQPRQPOPPPOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNIPNNNMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:64:11481:16674#0	0	chr3	58125472	60	100M	*	0	0	GTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGT	44267<=CA??D@@@CBA7@B??C>@@B?@BB@CAC?BB@?>?A??A@>BBBBBBAD@DBBB??>>?<BCB@@?B@?CBBB?B??7@CBAC?@BA=;=:9	BD:Z:IILLPPRNNONMNKLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@AAJKGIIAKLMMJJKLJAKILLNNMIAKLLNNKLKBKKOKLCLIJKI	MD:Z:100	RG:Z:0	BI:Z:MMPPQRSOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPQPQPOHQPQQQNPNHPPRNMGMMMOM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:68:15440:147801#0	1024	chr3	58125472	60	100M	*	0	0	GTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGT	44267<=CA??D@@@CBA7@@??C>@@B?@BB@CAC?@B@?>@B@ACB@DBBBBBAD@BBBB?@>>?BACB@A?B@?DBBB>B??7@@BAB?@BA=;;:9	BD:Z:IILLPPRNNONMNKLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@AAJKGIIAKLMMJJKLJAKILLNNMIAKLLNNKLKBKKOKLCLIJKI	MD:Z:100	RG:Z:0	BI:Z:MMPPQRSOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPQPQPOHQPQQQNPNHPPRNMGMMMOM	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:2:7421:116295#0	0	chr3	58125473	60	100M	*	0	0	TATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTT	4262;;BA>?D>@@DBA8@BA?D?AABAAC>@DAC?AA??=<>;A?>?B@CBCCBDAECCC?@??>A@CC@@>@@?DBB@?B@@6ACC@C?ACCA@>:<=	BD:Z:JJLLQRPNONNOJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIALLLNNLMKAKKOKLCLKKKJK	MD:Z:100	RG:Z:0.1	BI:Z:NNPPSSQQQQPQOPPNONPOPPNOQNOQPNHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPQRROROIPPQOOHNLNONN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:63:4580:108191#0	0	chr3	58125474	60	100M	*	0	0	ATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT	46258@@>>D@@@CCA7@B??C>AAA?@CB@DAC?@B@@>AAAACB@EBACBCBEAECCC?@>?>@CCC@A?BA?DCCC?C@@7ACC@C@ACC?@A;;>?	BD:Z:JJLNRQOOONOKLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKLLNNLMKAKKNKLBMKLKJKA	MD:Z:100	RG:Z:0.1	BI:Z:NNPQTQRQQPQOPPNOOPOPPNOPOOPQNGOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPRROROIPPQOOIOMLONNH	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:6:18428:11442#0	1024	chr3	58125474	60	100M	*	0	0	ATAGCATGCAGACTTCGACGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT	46248@@>>D@@@CBA7@@7?C?@@B?@BB@CAC?@B?@>?A?BCB@DBCCBCBEAECCC?A???ABCC@@?C@@FCCC?C@A8@CCBD@ACC?@A;;=?	BD:Z:JJLNRQOOONOKLKIJKMLKMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKLLNNLMKAKKNKLBMKLKJKA	MD:Z:18T81	RG:Z:0.1	BI:Z:NNPQTQRQQPQOPPNOOPONONOPOOPQNGOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPRROROIPPQOOIOMLONNH	NM:i:1	AS:i:95	XS:i:20
HWI-ST142_0240:1:1:7175:9732#0	1024	chr3	58125474	60	100M	*	0	0	ATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT	46248@@>>D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@A@=C?@B?BBBBAD@DBBB>@?>>ACBB@@?B@?EBBB?B??7@BBBC?@BB??@:;==	BD:Z:IILMSPPOONNKMLHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@JKHIHAKLMMJJKLJAKILLNNMIAKLLNNKLJAKKNJLCMKKKIJA	MD:Z:100	RG:Z:0	BI:Z:MMOPSQRPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPQOOHQPQQQNPNHPOQOOHOMKOMMG	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:4:12180:29132#0	16	chr3	58125474	60	100M	*	0	0	ATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT	89>:=AD??C@??B@8ABD?@?CACD@BB@@@@D@BCA?CAC@BB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>@D?7@BB@@D@BAC>>=4664	BD:Z:LLMNOOOPOKNOKJMKMMMOMLJMMKJAJNLKNKJILLLJJJIJLMNJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IKNIKBJJNLMKAII	MD:Z:100	RG:Z:0	BI:Z:PPNPNPOQRPQRPOQOQQPRQQOQPQOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLPPNNGMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:63:15535:184865#0	0	chr3	58125475	60	100M	*	0	0	TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTT	3031?>><@>99@A<64A?=C>?8BA>CC?C@?@AA?A:>9=?==:BAC==><B45?>?7==<<5/96>7=B@=B@,A?B@?6ACB@B@AC=@A?>>%%%	BD:Z:JJNOQPPOOOKMKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKLNNLMKAKKNJLBLLLLJKAA	MD:Z:100	RG:Z:0.1	BI:Z:NNQRRRRQPQOPPNOOQOPPNOPNPPPOGNPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQROROIPPQOOIPNMMNNHH	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:43:2406:80244#0	1024	chr3	58125475	60	92M8S	*	0	0	TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATACGTTTCTGTTTAGATCGAA	4243=>=>C@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@@@ABBADBBBBBAB@DBBB?@>>>ABCB?A?B@@CCCB?@&=5??@>C@<BB>@@?:+-9	BD:Z:IIMOQPQONOKMLIIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IKHII@KLMMJJKLJAKILLNNMIAKLLLLKLJAJKNJKCLOMMMKMJ	MD:Z:80G11	RG:Z:0	BI:Z:MMPQQRRPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPPNHQPQPPOPNHPOQNOIQPONPNPN	NM:i:1	AS:i:87	XS:i:20
HWI-ST142_0240:1:62:18232:60158#0	1024	chr3	58125475	60	100M	*	0	0	TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTT	3235=>:;@=>=@@?78A>>A>>A@@@B@@B@B@@B??=@:*=<=>B=@<BB>B@CBCB<?>?>A@BB8@>@A=BCBA?A=>7@BB@B@@BB@@8=;;49	BD:Z:IIMOQPQONOKMLIIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IKHII@KLMMJJKLJAKILLNNMIAKLLNNKLJAJKNJKCMKLLIJAA	MD:Z:100	RG:Z:0	BI:Z:MMPQQRRPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPPNHQPQQQNPNHPOQNOIPMMMMMGG	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:8:18333:60211#0	1024	chr3	58125475	60	100M	*	0	0	TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTT	4233=<=>C?@@CB?8@A??A?@AB?@BB@B@C?AB@??@A@@B@@DBBBBB@B@@AA@?@>>>AAB@@@?A@<CBBB?B=?8@@B@B@=BB?@A=;<;;	BD:Z:IIMOQPQONOKMLIIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IKHII@KLMMJJKLJAKILLNNMIAKLLNNKLJAJKNJKCMKLLIJAA	MD:Z:100	RG:Z:0	BI:Z:MMPQQRRPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPPNHQPQQQNPNHPOQNOIPMMMMMGG	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:5:18054:24089#0	0	chr3	58125476	60	100M	*	0	0	TGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTG	332:=;<B;@?BB@3@A@?A?@ABAACC>C@CAAC@A?@@@ABB@D@CCCC@E@CCBB>?????BCCA@[email protected]?B@?7@CC@CAACC@@C@??=>;	BD:Z:JJNNPQPOPKMLIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKNNLMKAKKNJKBLKMLKKAAK	MD:Z:0A99	RG:Z:0.1	BI:Z:NNQPSRRPQOPPNOOQPPPNOPNOQPNHNONOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQOROIPPQOOIPONNLNHHO	NM:i:1	AS:i:99	XS:i:20
HWI-ST142_0240:1:1:6358:13961#0	16	chr3	58125476	60	100M	*	0	0	AGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTG	<9:=B>?CA@@BA7ABD?@>CABDBBCA@A@DACC@>DABABBA?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD?AB<;6684	BD:Z:MNNMNPOLOOKKNKMMMOMLJMMKJAJNLKNKJILLLJJJJJKMNKA@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMIKAIJLJLNEAIII	MD:Z:100	RG:Z:0	BI:Z:NPPPMPPORSPOQOQQPRQQOQQQOHMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNNPIGNMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:22:14034:114749#0	0	chr3	58125477	60	100M	*	0	0	GCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGA	4386;=B?@@BBA7@B??C>@AB?@BB@CAC?ACA@??A??AA?BACCACBDADCCC??>?>@?CCAA?BA=CCCC?BA@7ABCBC@ABC@?CB?B?=:<	BD:Z:JJNMQQPPLMLJJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMNLMKAKKNJKALKLMKLAAKN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSRQQOPPNOOQPQPNOPNOPQNGOOMOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNROIPPQOOIPOOOMLHHOQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:27:14656:55962#0	1024	chr3	58125477	60	100M	*	0	0	GCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGA	3167;;B??>AB<6@@?<A>>>>=@AA>@@@?9A==;>?>9<99<B@BBB@B?BA<=>?;0>@?5:83<1>7<87@>9?>2<@?@CA?A<@A@@@-<559	BD:Z:IIMMRQPOLNLIJKLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGIIAKKMMJJKLJAKILLNNMIAKLLNNKLJAJJMJKBLKLMKKAAKM	MD:Z:100	RG:Z:0	BI:Z:MMOPRRQPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONHPPQQQNPNHPOQNNHQONOMKGGOP	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:43:9839:192700#0	1024	chr3	58125477	60	8S92M	*	0	0	GCTGTGTGGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTCT	43739;>>=?DB??D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@A@@BB@DBBBBBAD@DCBB?A>>?BBCC?A>B??DBBB>B?@7@B@AB>%%%%%	BD:Z:IIMMMLLJNNMMNNLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJMJAA@JKHIIAKLMMJJKLJAKILLNNMIAKLLNOLMKCLLOJJAJJ	MD:Z:90G1	RG:Z:0	BI:Z:MMNPOOOMOOOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGPMQQQQPOHQPQQQNPOIPNPKMGON	NM:i:1	AS:i:90	XS:i:20
HWI-ST142_0240:1:25:16127:188276#0	0	chr3	58125478	60	100M	*	0	0	TATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG	42638@>??CBA7@B??C>@@B?@BB@CAC?AB@@?@B@ABA@DBBBBBAD@DBBB?@>>>A@BB?A?A@@CBCB?B@?8=BBAC?@CB?@B?@A@=9;9	BD:Z:IILNRPQLNMIJKMKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHIAKLLMJJKLJAKILLNNMIAKLLNNKLJAJJMIKBLJLMKLBAKML	MD:Z:0C99	RG:Z:0	BI:Z:MMPPRQROPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGQOQQQNPNHPOQNNHPOOPMMEGOPO	NM:i:1	AS:i:99	XS:i:20
HWI-ST142_0240:1:5:13896:154389#0	1024	chr3	58125478	60	100M	*	0	0	CATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG	48638@>??CBA7@B??C>@@B?@BB@CAB?@B@@?@A@@B@@DABBBB@D@DBBB>@>>>ABBB@@>B??CBCB?B??8@BBAC?@BB?@B?@A@=:;;	BD:Z:IIMNRPQLNMIJKMKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHIAKLLMJJKLJAKILLNNMIAKLLNNKLJAJJMIKBLJLMKLBAKML	MD:Z:100	RG:Z:0	BI:Z:MMPPRQROPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGQOQQQNPNHPOQNNHPOOPMMEGOPO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:64:15528:95249#0	1024	chr3	58125478	60	100M	*	0	0	TATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGGTTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG	42638@>??CBA7@B??C>@@B?@BB@CAC?@B@@?@@@@BB@DBBBBBAD@DBBB>@>>>>@BB?@?B@@DBCB?B?>8=BBAC?@CB?@B?@A@=9;9	BD:Z:IILNRPQLNMIJKMKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHIAKLLMJJJLJAKILLNNMIAKLLNNKLJAJJMIKBLJLMKLBAKML	MD:Z:0C60A38	RG:Z:0	BI:Z:MMPPRQROPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJJOMGOMPPPPONGQOQQQNPNHPOQNNHPOOPMMEGOPO	NM:i:2	AS:i:94	XS:i:19
HWI-ST115_0148:1:3:18398:22371#0	16	chr3	58125478	60	100M	*	0	0	CATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG	;==;>A>A?C46@BC???C@BD@DCA?B@E@CC@<D@CADBA@BCA@A?@@ACCB?C???@BCCD@<BE=ABCB>AE?5@CC@?E?CCEABC@A@=:463	BD:Z:NMNNOLPOLLNLNMNONLKNMKJ@JMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIIKJJLDEMLKJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPQNNQRQPRPRRQSRQPQQPOHNQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQNGOOOQOONNGNNQMOHNMONMOIIPPNNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:48:12854:139828#0	1040	chr3	58125478	60	100M	*	0	0	CATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG	8<?:=BA@@B@7BBD?@>CABDABC@ABAEBBCA>CABABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD>7@BB@@D@BBD@BC?@>;8574	BD:Z:NMMNNLOPLKNLNMMOMLJMMKJAJNLKNKJILLLJJJJJLMMKAAAILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJAIIKJJLDDMKJII	MD:Z:100	RG:Z:0	BI:Z:PPOPNNPRQPQOQQPRQQOQQQPHMROOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNIIPPNMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:41:13159:188033#0	0	chr3	58125479	60	100M	*	0	0	ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGC	4633=<>?BBA7@B??C>@@B?@BB@CAC?@B@@>@@@@BB@DBBBBBAD@DBBB>@>>>>BBB?@?B?@CBAB?C??7@@BAC?@BC?@B?@BA@:;;8	BD:Z:IINNQQNNMJJKMLMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHHAKLMLJJKLJAKILLNNMIAKLLNNKLJAJJMIJBLJKMKLCBKMLO	MD:Z:100	RG:Z:0	BI:Z:MMPPQRQPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPPPQQNPNHPOQNNHPNOQNMGEOPOQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:6:10123:75568#0	1024	chr3	58125479	60	100M	*	0	0	ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGC	4533=<>?BBA7@B??C>@@B?@BA@B@C?@B@@>?A?ABA@DABBBBAD@DBBB?@>>>@BCB?@?@?@CCB@?C??7?BBAC?@BC?@B@@BA@:;:8	BD:Z:IINNQQNNMJJKMLMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHHAKLMLJJKLJAKILLNNMIAKLLNNKLJAJJMIJBLJKMKLCBKMLO	MD:Z:100	RG:Z:0	BI:Z:MMPPQRQPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPPPQQNPNHPOQNNHPNOQNMGEOPOQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:7:13079:161590#0	1024	chr3	58125479	60	100M	*	0	0	ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGTGTTTCTGTTTGTTGTTTTGAGC	4433=<>?BBA7@B??C>?@B?@BB@CAC?@B@@?@A@ABB@DBBBBB@D@CBBB>@>>>@BCB?@?B?@DCBC?A@1@@9B@CA@BC?@B?@BA@:::8	BD:Z:IINNQQNNMJJKMLMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHHAKLMLJJKLJAKILLNNMIAKLLNIIIJAJJMIJBLJKMKLCBKMLO	MD:Z:77C22	RG:Z:0	BI:Z:MMPPQRQPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPPPQNNNNHPOQNNHPNOQNMGEOPOQ	NM:i:1	AS:i:95	XS:i:20
HWI-ST142_0240:1:41:10866:49403#0	16	chr3	58125479	60	100M	*	0	0	ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGA	=?:;A@@@BA7@CD?@>CABDABB@@A@EBCB@?BACBBBA?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD?A@>=5754	BD:Z:MMNMKOPLLNLNNMOMLJMMKJAJNLKNKJILLLJJJJJLMNJAAAJLHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@IIKIJLBDLOJJII	MD:Z:99C0	RG:Z:0	BI:Z:POPPLPQPPROQQPRQQOQQQPHNQPOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGIPRNNMM	NM:i:1	AS:i:99	XS:i:20
HWI-ST115_0148:1:5:2026:25837#0	0	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA	33389=>BBA7AB?@C>@@B?ABBACAD?@BA@>@A?ACB@DBCCBC@E@DBAC?@=>?@BCAAA?CA?DCCB?BAA9ACC@D@ACCAAC?ACCA?>:7=	BD:Z:JJNNRNOMKKLNLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLKAKKNJKAKJKLKLCBLNLON	MD:Z:100	RG:Z:0.1	BI:Z:NNQPSPQPNOOQPQQOPPNOPPNGOOMOOPOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHPPQOOIPOOPOOHGMQPRP	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:68:7477:156944#0	1024	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA	43299<>BA?7@B?>@><=A?AB?@B>B?>A>A?@A>@?>>B?@@BB>D=BB@@=?>>=ABB@?A>BA?AB6@>@@>7@B=@A@@BB@@A@@BBA>=:.=	BD:Z:IINMRNPMJKKMLNMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@KLMMIJKLJAKILLNNMIAKLLNNKLJAJJMIJALJKLKLCCLMLOM	MD:Z:100	RG:Z:0	BI:Z:MMPORQRPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOQPQNPNHPOQNNHPNNQONGGMPOQO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:7:16006:51811#0	1024	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGCTTCTGTTTGTTGTTTTGAGCA	43389<>BAA7@B??C>@@B?@BB@CAC?AB@@>@A@@BB@DBBBBBAD@DBBB>@>>>ABBC?@>C??CBCB?B@?8?BBAC?@BB?@A?@BBA=<:8=	BD:Z:IINMRNPMJKKMLNMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@KLMMIJKLJAKILLNNMIAKLLNNKMMIJJMIJALJKLKLCCLMLOM	MD:Z:78T21	RG:Z:0	BI:Z:MMPORQRPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOQPQNPOOPOQNNHPNNQONGGMPOQO	NM:i:1	AS:i:95	XS:i:20
HWI-ST115_0148:1:5:5308:32801#0	16	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA	>::>@@@BA8BCE@A?CBBE?DCABA@E@CDA?D?D@CBA@BCCCCA@A@BCB>C@?@ABBCD@>BD??BBB>BD?7@BB@@D@BBD@BD@AA@@:7334	BD:Z:NNNKOOMLOMNMNONLKNMLK@JMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJJIKCCLONNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQPOOPPPRPRRQSRQPRRPOHNQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHNOPPOPNNGNNPLOHNMONMOHHOQPOQNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:22:2585:58573#0	1040	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA	:::>??@BA7ABE?@>CABD@BB@@AADBCD@>DACACB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@AA@@:7334	BD:Z:MNMJNPLLOLNNNOMLJMMKJAJNLKNKJILLLJJJJJLMNK@AAJMHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HIKIILBBLNNMNII	MD:Z:100	RG:Z:0	BI:Z:OPPNNQOORPQQPRQQOQQQPHNROPPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGPRPNPMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:44:11750:170821#0	1040	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA	=::=>?@B@7@BE?@>CABDABBAAA?E@CD@?DABACAA?BBBBB@?@?BBB>C@>>ABBBD@>BD??BBB>BD?7?BB@@D@ABD@BC?AA@@:7324	BD:Z:MNMJNPLLOLNNNOMLJMMKJAJNLKNKJILLLJJJJJLMNK@AAJMHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HIKIILBBLNNMNII	MD:Z:100	RG:Z:0	BI:Z:OPPNNQOORPQQPRQQOQQQPHNROPPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGPRPNPMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:46:11710:142327#0	1040	chr3	58125480	60	100M	*	0	0	TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA	?98>??@B@8ABE?@>CABD@BB@@@AEBBD@>DACACB@?BBBBB@?@?BBB>C@@@ABBBD@?BD??BBB>BD?7@BB@@D@BBD@BC@AA@@:7334	BD:Z:MNMJNPLLOLNNNOMLJMMKJAJNLKNKJILLLJJJJJLMNK@AAJMHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HIKIILBBLNNMNII	MD:Z:100	RG:Z:0	BI:Z:OPPNNQOORPQQPRQQOQQQPHNROPPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGPRPNPMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:64:19711:152416#0	16	chr3	58125480	60	10S90M	*	0	0	TTCCGATCTTTGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT	7961<1?>BBB>@B@@?BA6@BD>@?B@BD>@B=?@@D@BC@?C@C@@@@@BBBBABA8?BB>?A>>>@>BBB=>>D??BBB??D=6>AA@>B>>;8354	BD:Z:JKLKNNMLCJNONJMNJJMKMMMOMLJMMKJAJNLKNKJILLKJJJJIKLMJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLKKAJLOJNDMIKII	MD:Z:90	RG:Z:0	BI:Z:NPMNNPOPIPPQQOQRPOQOQQPRPQNPPPOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLPIPLNMM	NM:i:0	AS:i:90	XS:i:20
HWI-ST142_0240:1:44:16206:105260#0	0	chr3	58125481	60	100M	*	0	0	GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG	3275;<?@>5><<2=>>@@?@BA?B@B>AB8;=<???A@>B=@@@B@D@DBB@???>>@?AB@@?@?=DABB?B@?7@BB=B@@?BA?>>9?2:=?;885	BD:Z:IIMNOPOJKLMLNNLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JLMMJIKLJAKILLNNMIAKLLNNKLJAJJMIJAKJKLJLCCMNLOMN	MD:Z:100	RG:Z:0	BI:Z:MMOPQRRNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPQPNPNHPOQNNHPNNPOOHGONOQOP	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:65:13196:26849#0	1024	chr3	58125481	60	100M	*	0	0	GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG	43849<AA@7@B??C>@@B?@BA@CAC?@B@@>@@?ABA@DBBBBBAD@DBBB>@>>>@BBB@@>B@?DBCC?C??7@BBAC?@BB?AB?@BBB>?;8>6	BD:Z:IIMNOPOJKLMLNNLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JLMMJIKLJAKILLNNMIAKLLNNKLJAJJMIJAKJKLJLCCMNLOMN	MD:Z:100	RG:Z:0	BI:Z:MMOPQRRNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPQPNPNHPOQNNHPNNPOOHGONOQOP	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:27:14769:95346#0	16	chr3	58125481	60	100M	*	0	0	GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG	:;=<?>3A7@BD???C@AD@CDABA@EAADA?B@D@?B@ABCBCBA?@@BCB?D??=ABCCE@?BD?@:CB?BD>5@ACAAEABCE@CEABBBC>>4463	BD:Z:NNKNOLMOMOMNONLKNMLKAJMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIIKBCKNNQNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPOQOOORPRRQSRQPRRQOHNQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOOOQOOONGNNPLNHNMONMOHHNQOQQPNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:67:19258:137763#0	1040	chr3	58125481	60	100M	*	0	0	GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG	89;;>?BA7@BD???C@BD>BB@@@?EBBBA>BBCABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@AD@BAAB=<3374	BD:Z:NMJMOLLOMNNNPMLJMMKJAJNLKNKJILLLJJJJJLMNKA@AJMIHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHKIIKBBJNMQNMII	MD:Z:100	RG:Z:0	BI:Z:PPNPOONQPRQPRQQOQQQPHNRPOQPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNRPPPPMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:8:18119:188547#0	0	chr3	58125482	60	100M	*	0	0	TAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATCTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGG	42449?@?6@A??C>@@B?@BB@AAC?@B@@?@A@ABB@DBBBBBAD@DBBB>@>>>:A@A@@?A@?CBBB?A@>8@BB@C?@BB?@B?>BBB@?:9<85	BD:Z:IIMKQOLKLNLNNMKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKMMJJJLMJMILLNNMIAKLLNNKLJAJJMIJAKIKLJKCCMOMOMNM	MD:Z:0C58T40	RG:Z:0	BI:Z:MMPORRPONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPPNPMPPPPONGPOPPQMPNHPOQNNHPNNPNOIHOPMQOPO	NM:i:2	AS:i:94	XS:i:19
HWI-ST115_0148:1:24:6939:189286#0	0	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444<>?6?B??C>@@B?@BB@CAC?@B@@?AAAACB@EBBBBBAD@DCBC>A???ACCC@A?C@@DCCC?C@?8ACCBD@ACC@@C@ACCC@A@?A;9<	BD:Z:JJKMOMMMOMNNMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JKNJKAKJKKJKBBLPMPNONM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPROPOQPQQOPQOPPPNGNOMOOPOOPQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPOOIPOOPOOIIPQOPPQOP	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:28:5159:97290#0	1024	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444>>?6@B??D?@AA?@BCAAAC?AAA@>?@>ACB@DBCCBCAE@EBCC?A??>AACB@A?BA>DCCB?BA@7@DCBB@ACC@AB@@CCC@=@>A:8<	BD:Z:JJKMOMMMOMNNMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JKNJKAKJKKJKBBLPMPNONM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPROPOQPQQOPQOPPPNGNOMOOPOOPQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPOOIPOOPOOIIPQOPPQOP	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:23:11033:120892#0	1024	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444<>?6?B??C>@@B?@BB@CAC?@B@@?@A@ABB@DBBBBBAD@DBBB>@>=>A@BB?A?B??DBBC?B@@7ABBAC?@BB?AB?@BBB?A>=?:9;	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML	MD:Z:100	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:23:5024:179493#0	1024	chr3	58125483	60	98M2S	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGAG	3246<>=5?@><B?<@B>@BA@C?B?@B@?>?A>?B5=B@>;<>;;>:<664+;6?BBBB?9>??>B6A@?A@?7@B@@B;@BBA@B@@9BB:<>=?734	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNKL	MD:Z:98	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:98	XS:i:20
HWI-ST142_0240:1:2:14618:76577#0	1024	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444<>?6?B??C>@@B?@BB@CAC?@B@@?@A@@BA@DBBBBBAD@DBBB>@>>>@BBB?@?B??DBBC?C@@7ABBAC?@BB?@B@@BBB@@?=?;97	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML	MD:Z:100	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:46:19190:95037#0	1024	chr3	58125483	60	98M2S	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGAG	4444<>?6?B??C>@@B?@BB@CAC?@B@@??A??A@>B@BBBBAD@DBBB?@>>>ABBB?@?B??CBAB?C@@7ABBAC?@BB?@B@@BBB?A?=?;;6	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNKL	MD:Z:98	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:98	XS:i:20
HWI-ST142_0240:1:47:11226:2414#0	1024	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444<>?6?B??C>@@B?@BB@CAC?@B@@?@@@@BA@CABBBBAD@DBBB>@>>>ABBB?@?B??CBBB?C@@7ABBAC?@BB?@B@@BBB?B?=?;99	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML	MD:Z:100	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:6:10870:168832#0	1024	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444<>?6?A??C>@@B?@BB@CAC?@B@@??A?<BA@DBBBBB@D@DBBB>@>>>@BBB?@?B??BBBB?C@?7ABBAC?@BB?@B@@BBB?@?=?;89	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML	MD:Z:100	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:8:3362:36600#0	1024	chr3	58125483	60	100M	*	0	0	AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT	4444<>?6?A??C??@A?@BA@C@C?AB@>??B?BBA@CABBBBAD@DBBB>@>>>@@BBA@>:@>CAA@?B@?7ABBAB=A@B?AB@?BBB?A?=?777	BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML	MD:Z:100	RG:Z:0	BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:45:20043:38425#0	0	chr3	58125484	60	100M	*	0	0	GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC	4447;=5?A??C>@@B?@BB@@AC?@B@A>?A@ACB@EBCBBBAD@CBBB?@???ABCC@@?CA@DCCB?BA>7ACCBD@@DC@AC@ACCC@@A?D;9<;	BD:Z:JJMLMNNONNNNKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJNJKAKJKKJKABLONPOONMN	MD:Z:100	RG:Z:0.1	BI:Z:NNPPPPPQPQQOPQOPQPNGNOMNPPNPPPQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNOIPOOPOOIIPRPQNQOPQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:41:16470:171070#0	1024	chr3	58125484	60	100M	*	0	0	GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC	4437;<6??>>C>@@A?@AB@A@A@@B@???A@BBA@DABBBB@C@DBBB>?>>>;ABB@@?A@>CB;A?BA?7@BB@C?=B@?@@?@>BB?A?>@;7:9	BD:Z:IIMLMMNNMONMLMJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLJJKLIAKILLNNMIAKLLNNKLJAJJMIJAKIJKJKBBMONQNNMLM	MD:Z:100	RG:Z:0	BI:Z:MMPPPQPPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMPMHPOQNNHPNNPNNHIQQOQMPOOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:47:19503:63851#0	1024	chr3	58125484	60	100M	*	0	0	GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC	4447;=5?A??C>@@B?@BB@CAC?@B@?>=@>9@@?A?@BBBAD?DBB@>@>>>ABBB?@?B??CCB@?A@?7@BB@B@7@???@??>>B?B@>B;8;9	BD:Z:IIMLMMNNMONMLMJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLJJKLIAKILLNNMIAKLLNNKLJAJJMIJAKIJKJKBBMONQNNMLM	MD:Z:100	RG:Z:0	BI:Z:MMPPPQPPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMPMHPOQNNHPNNPNNHIQQOQMPOOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:22:18100:32416#0	16	chr3	58125484	60	100M	*	0	0	GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC	=;;>?7@BE@@?DBCFA>DAAABF@CDA?D@DBCCA@CCCCCA@@@CCB?C@@@ABBBD@?BD??BBB>BD@7@BB@@D@BBD@BD@BBBD@B=;<4444	BD:Z:NNKKOMPNOPNLKNMLKAKNLJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJABJLLOPQOMMJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPOONQRQSRQPRRQPIOQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQOOONGONPLNGMLONMOHHNPNORQROPNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:41:13824:159325#0	0	chr3	58125485	60	100M	*	0	0	ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT	4476:3>A@?C?@AB?@CB@C@C?ABA@>@A?ACB@DBCCBBAE@DBCB?A>?>ABCCAA?BA@DCCB=BA?7ACDBC@@DC@AC@ACCC@@A?D?;;;@	BD:Z:JJLJNOPNONNLMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMJKAKJKKJKAALOMQOPNMNK	MD:Z:100	RG:Z:0.1	BI:Z:NNPNQPRPQQOPQOPQQNGNOMNOQNOQPPOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNIPOOPOOIIPRQROOOPQO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:67:14854:93529#0	1024	chr3	58125485	60	100M	*	0	0	ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT	4476:3>A>@C>@@B?ABB@CAC?@C@@?@A?ACBAEBCCBC@D@DBCB>A>?>ACCC@A?B?@EBCC?C@?8ACDBE@@CC@AC@ACBC@AA?E>:;;=	BD:Z:JJLJNOPNONNLMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMJKAKJKKJKAALOMQOPNMNK	MD:Z:100	RG:Z:0.1	BI:Z:NNPNQPRPQQOPQOPQQNGNOMNOQNOQPPOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNIPOOPOOIIPRQROOOPQO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:7:1891:111214#0	1024	chr3	58125485	60	100M	*	0	0	ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT	4476:3>A>?C>@@B?@BB@CAC?@B@@>@A@@BB@DBBBBB@D@DBBB>@>>>BBBB?@?B@?DBBA?C??8@BB@C???B?@B?@CBB?@??C<9;<>	BD:Z:IILINNPMOOMLMKKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIJKLJ@KILLNNMIAKLLNNKLJAJJMIJAKIJKIKBBLONQOOMLMJ	MD:Z:100	RG:Z:0	BI:Z:MMPNQPRPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMONGPOQNNHPNNPNNHHQRPQONOOPN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:1985:183505#0	16	chr3	58125485	60	100M	*	0	0	ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT	9:<<5?BD>@?C@BD@BB@>@@D@BB@?B@B@BB@@@BBAB@?@?BBB?C@@>@BABD@?BD??BBA=AD?8@BB@@D@ABD>BD@BBBC?B>=?94334	BD:Z:NJJMLOOOQNMKNMKJAJNLKNKJILLLJJJJJLMNKAAAJMHIKAJKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJAAIKKNOPOPMKII	MD:Z:100	RG:Z:0	BI:Z:QONPLPPPSRQOQQQPHNRPPQQPNQOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPRRQPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:67:14903:30979#0	0	chr3	58125486	60	100M	*	0	0	CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC	47650<@>>C>@@B?@BB@CAC?@B@@>@@AACB@DBBBCBAE@DCAB>>?>>ABCCAA?BA@ECCB?BA@8ACCBC@@CC@AC@ACCC@@@?E@<=:>:	BD:Z:JJJKOQOOONLNJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIKAKJKKJKAAKOMPPPOMNKK	MD:Z:100	RG:Z:0.1	BI:Z:NNNOQRQQQOPQOPQQOGNOMNOPOOPQPNPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHPOOPOOIIPRQSPPMPQON	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:8:17184:151826#0	1024	chr3	58125486	60	100M	*	0	0	CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTA	37651=?>>A>=@B@@BC@C>CA>C>>>=A?ABB>@A?>BC@E@<CCA=?>==@BCCA@?B?:>A<B=BA=7A@A;:=99C@@@@@BCCA3=>@?;=9<8	BD:Z:JJJKOQOOONLNJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIKAKJKKJKAAKOMPPPOMNKL	MD:Z:99C0	RG:Z:0.1	BI:Z:NNNOQRQQQOPQOPQQOGNOMNOPOOPQPNPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHPOOPOOIIPRQSPPMPQOP	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:24:10894:139937#0	1024	chr3	58125486	60	100M	*	0	0	CTTCGATTCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC	47550=@A@C>@@=?@BB@CAC?@@@@=@@@ABB@DBBBBBAD@DBBB?@>>>@BBB?@?B?@BBAB<A@>7@BB@C??BB?@B?@BCB?@>?D?<;7;<	BD:Z:IIIJOPOKKKLMKLMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIKLJAJILLNNMIAKLLNNKLJAJJMIJAKIJKIJBBLNNQOPNLMJJ	MD:Z:7G92	RG:Z:0	BI:Z:MMNOPRRNONPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMHOOQNNHPNNPNNHHPRQROPMOPNN	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:4:3218:151835#0	1024	chr3	58125486	60	100M	*	0	0	CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC	46543<@>>B>@@<?@BB@B@C?@B@>?@@<8A>=CABBBB?D@DBBB?@>>>AABB@@?A??DBB@?BA?8A@@@C@AB@?@B??BBB?>??B>;<9:7	BD:Z:IIIJOPOOONLMKLMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIKLJAJILLNNMIAKLLNNKLJAJJMIJAKIJKIJBBLNNQOPNLMJJ	MD:Z:100	RG:Z:0	BI:Z:MMNOPRRPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMHOOQNNHPNNPNNHHPRQROPMOPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:7:15227:67080#0	1024	chr3	58125486	60	100M	*	0	0	CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC	47650<@>>C>@@B?@BB@CAC?@B@@>@@?@BA@DB@BBBAD@DBBB>@>>>ABBB?@>B?@DBBC?B@?8@CBBB?@BB?@B?@BCB?A@?D?<;<7<	BD:Z:IIIJOPOOONLMKLMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIKLJAJILLNNMIAKLLNNKLJAJJMIJAKIJKIJBBLNNQOPNLMJJ	MD:Z:100	RG:Z:0	BI:Z:MMNOPRRPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMHOOQNNHPNNPNNHHPRQROPMOPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:62:18957:42396#0	16	chr3	58125486	60	100M	*	0	0	CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC	:;;3=BD@B?DBBE@DC?BA?FBCD@?DBD9CCA@BCCCCA@AACCC?CA@?ABCBFA>BD@?BBB>BE?7@BB@@D@BBD@BD@BBBDAC>>A<94444	BD:Z:KKNLONPPOMKNMLKAKNLKOKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAILKNOONPPKLJJ	MD:Z:100	RG:Z:0.1	BI:Z:POQOOPPSRQPRRQPIORQOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPNNHNNQLNGMLNMMOHHNPNOQPQPROPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:27:20341:130547#0	0	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGTGCAGGTGTCC	063-<<>=7>>?A@@B?>C@B@?C?=<=><ACB?AAA>>>=C>>ABB???59<AAC@A;B@??CCB<1??39<=<CBACCAAB@A%%%%%%%%%%%%%%%	BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKJJOOQONIJNM	MD:Z:89A6C3	RG:Z:0.1	BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPONRQQNNMNQO	NM:i:2	AS:i:91	XS:i:20
HWI-ST115_0148:1:45:19946:11303#0	1024	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	364,;?=<B?>=A@ABB?C@CAAC@>:>=?4@=8??B@=@AC@ACAA=??>?@ACC@A?B@>BBC@?BA?7A@B@C@ACCA@CAACD<@A=<B@<?;=;8	BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKNMPOQONNKKM	MD:Z:100	RG:Z:0.1	BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPRQSQQNNQONO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:47:9971:161282#0	1024	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	363,;>=?>=>@A@?BB=@@D?>C>?68::9>?67?A?A?@E@>CAB>=+52>;@CA@?B?<C?+B=@4=8@CB@C?ACC@AC?>CBC@A@5C3>?:<97	BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKNMPOQONNKKM	MD:Z:100	RG:Z:0.1	BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPRQSQQNNQONO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:6:6832:181239#0	1024	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	365+;>=>C?A@AAABB@CAC?AB@A??A?>BA?CBCCCCADADCBB>@>>>ABCD@A?CA@ECCC?B@?7ACC@E@ACC@AD?ACDC@4@>D=<?<<;7	BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKNMPOQONNKKM	MD:Z:100	RG:Z:0.1	BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPRQSQQNNQONO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:22:11619:171791#0	16	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAGACTGTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	>=2=AD?A?DABEBD>@?D*EBCCB?DBC@CBA@BCCCCA@A@CCC?D@A@ABBCD@>BD??BBB>BD@7@BB@@D@CBD@CD@BBBDAC?>A><94424	BD:Z:KNLNNOQOMLNMLKKNNOIILJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKKNNOMOPNLLJJ	MD:Z:15A3C80	RG:Z:0.1	BI:Z:OQOQOORRQPRRQOPRRRNMPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPONGONPMNGMLNMLOHHNPNOQPPPQQPPNN	NM:i:2	AS:i:90	XS:i:20
HWI-ST115_0148:1:63:20138:120245#0	1040	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	<<2;AD???DABEBCD@?BBEBCDA?A@DBCCA@CCCCCA@A@CCC?D@A@ABBBE@?BD??BBB>BD?7@BB@@D@BBE@BD@BBBDAC?>B><94424	BD:Z:KNLNNOQOMLNMLKAKNLKOLJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKKNNOMOPNLLJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQOQOORRQPRRQPIORQPQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPONGONPMNGMLNMLOHHNPNOQPPPQQPPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:6:10123:95999#0	1040	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	>=1<@D???C>@C>=B=?A@E@CC@?C@A@=4AABBCCCAAA@CBB?C>?=>B??B@<BD9@CCB?BE?5@CCA@A?;>>>AB=@CCC?B>9@<<<45-3	BD:Z:KNLNNOQOMLNMLKAKNLKOLJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKKNNOMOPNLLJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQOQOORRQPRRQPIORQPQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPONGONPMNGMLNMLOHHNPNOQPPPQQPPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:1:14398:125110#0	1040	chr3	58125487	60	100M	*	0	0	TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC	;;2;@C?@>C@AA@BB@@@@DABC@?DBCBCB@@BBBBBA@@?BBB>B@@@@BBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC?>B><94314	BD:Z:JMKMNOQONKNNLJAJNLKNKJILLLJJJJJLMNKAAAJMIIJAJLLKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@IKJMONMOPOLKII	MD:Z:100	RG:Z:0	BI:Z:NPNPNOQQRPQQQPHNRPPQQPOQOQNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPQRQOPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:68:17268:187738#0	0	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAGCTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	45+4;<=C>@@B?@C<>C@C?AB@@??A??CB@DBBBBCAD@DCCB?A?>>>BCC@A?@A@ECCB?B@@7ACB@?@ACC@ACA@CCB@@??C@8@??;:=	BD:Z:JJLNPQPOMNKMMMJJNMJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@KJKKJKAAKNLPOPPNOKKMM	MD:Z:15A84	RG:Z:0.1	BI:Z:NNOQRRROPQOPQQOPRNMNOPNOQPPOONOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONOPOOIIPRQSQROOOONOQ	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:68:4020:121175#0	1024	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	45+4;;=B=@@B?@BB@CAC?@BA@?@A@ACB@EBBBBBAD@DBBB>@>>?ACCC@@?B@@ECCC?BA@8@CC@C@ACC@@C@@DCC@A?>F??@@?;;>	BD:Z:JJLNPQPOMNKMMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@KJKKJKAAKNLPOPPNOKKMM	MD:Z:100	RG:Z:0.1	BI:Z:NNOQRRROPQOPQQOHOOMNOPNOQPPOONOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONOPOOIIPRQSQROOOONOQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:23:11356:171933#0	1024	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	45+4;;=B=@@B?@BB@CAC?@B@@?@@>@BB@DBBBBBAD@DBBB>@>>>ABBB?@>B??DBBB?C??8ABBBB@@BB?@B?@BBB@A@?D@>??><:?	BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL	MD:Z:100	RG:Z:0	BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:28:7496:9381#0	1024	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	32+9<;9?<=@>@=@<:@:?==@5>:696><83@6@A>@<A?BCAB5<>>=@9@@A??=><1<@@?B>=6>4@>9??@;?;@?AB:@:.>>B?%=9=439	BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL	MD:Z:100	RG:Z:0	BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:46:17107:126330#0	1024	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	45+4;;=B=@@B?@BB@CAC?@B@@?@@@?BA@DBBBBBAD@DBBB>@>>>ABBB?@>B??DBBB>C??8@BB@B@@BB?@B?@BBB?A@?D@>??><;?	BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL	MD:Z:100	RG:Z:0	BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:48:18179:38080#0	1024	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	45+4;;=B=@@B?@BB@C@C?@B@@?@A@>BA@CABBBBAD@DBBB>@>>>ABBB?@>B??DCBB>C??8ACCBB@@BB?=B?@BBB@@@?D?>?>><8?	BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL	MD:Z:100	RG:Z:0	BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:8:11819:138804#0	1024	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	45+4;;=B=@@B?@BB@CAC?@B@@>?A?<@?>A>BBBBAD@DBBB>@>>>ABBB?@>A??CCBB>C??8@BC@B@@BB?@B?@BBB@A@?D???<>:8:	BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL	MD:Z:100	RG:Z:0	BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:6:5048:176068#0	16	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	;2;>D@??D@BEADCAA@BFBCDB?DBCACCA@BCCCCA@A?CCB?DA@AABCBE@>BD@?BBB>BD?7@BB@@D@CBD@BD@BBBDAC??B?><94234	BD:Z:NLNMOPPMLOMLKAKNLKOLKIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJNNNMNONOLMJJ	MD:Z:100	RG:Z:0.1	BI:Z:QOQQNQQQPRRQPIORQPRPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOGNOPLOGMLNMLNHHNPNOQPPOQPRPONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:26:9631:21633#0	1040	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	82<=B>@>C@BDBBBA@B?AABC@?CBCBCC@?CBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBADAC??B?><94234	BD:Z:MKMMNQONLNNLKAJNLKNKJILLLJJJJJLMNKAAAJMIIK@JLLLIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HKJMNNMMPNPKMII	MD:Z:100	RG:Z:0	BI:Z:PNPPMQPQPRQQPHNRPPQQPOQPPONNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPORQQPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:2:15091:184337#0	1040	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	71:=B>?>C@BD@BB@@@@A@BC@?C@C@B@A@ABBBAA?@?BBB?C>@>@ABBD@?BD??BBB?BD?7@BBA@D>ABD>BD?BBBC@C>>B<><94233	BD:Z:MKMMNQONLNNLKAJNLKNKJILLLJJJJJLMNKAAAJMIIK@JLLLIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HKJMNNMMPNPKMII	MD:Z:100	RG:Z:0	BI:Z:PNPPMQPQPRQQPHNRPPQQPOQPPONNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPORQQPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:48:9330:156263#0	1040	chr3	58125488	60	100M	*	0	0	TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA	:2<=B>@>CABDBBB@@@@AABC@?CBD@CC@?CBBBC@?@?BBB>C@@@ABBBD@>BD??BBB>BD?8@BBA@D@BBD@BD@BBBCAC??B?><94234	BD:Z:MKMMNQONLNNLKAJNLKNKJILLLJJJJJLMNKAAAJMIIK@JLLLIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HKJMNNMMPNPKMII	MD:Z:100	RG:Z:0	BI:Z:PNPPMQPQPRQQPHNRPPQQPOQPPONNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPORQQPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:27:7074:100817#0	0	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	4+468;A=?@B?@BB?CAC?@C@@>@A@ACBAEBCBBBAD@DCCB>@>>>ABCC@A?BA@ECCB?CA@7ACCBD@ACC@@C@@CCC@A@@D=?@AA=8?=	BD:Z:JJNMQQPMOKMNMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JJKKJKAAKNLOOPOOOLKMMM	MD:Z:100	RG:Z:0.1	BI:Z:NNQPSRPPQOPQQOHOPMNOPNOPQPNPNNPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNPOOIIPRQSQRPPPMNOQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:28:19225:82436#0	1024	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	3+469=B=?@A?@CC?DAD?AC?A?>>=8@??CAACCCAD@CCAB?A>>>?BCCA@?BA?DBBB?B?>7ACC@C@ACC@@B@ACBC@A?>B=<@<@<8=<	BD:Z:JJNMQQPMOKMNMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JJKKJKAAKNLOOPOOOLKMMM	MD:Z:100	RG:Z:0.1	BI:Z:NNQPSRPPQOPQQOHOPMNOPNOPQPNPNNPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNPOOIIPRQSQRPPPMNOQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:22:14768:183117#0	1024	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	2*46::?<=@A?@AA?A?A@@B?>??A=AB>>A@BBBB@C@BB?B??>???@B@@@?@@>C=C@=BA?7@A>?B@@B@@A6@@B@B@A??D?>>??:7;8	BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIJKIJAAKNMPNPONOKJLLM	MD:Z:100	RG:Z:0	BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNNPNNHHPQPRQRPOPLNOPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:3:14548:68998#0	1024	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	4+468;A=?@B?@BB@CAC?@B@?>>@?@BA@CABBBBAD@DBBB>@>>>ABBB?@>B@?DBCB?B@?7@BBBB?ABB?@B?@BBB?A@?D@>@@?<;?=	BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIJKIJAAKNMPNPONOKJLLM	MD:Z:100	RG:Z:0	BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNNPNNHHPQPRQRPOPLNOPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:43:4125:134378#0	1024	chr3	58125489	60	97M3S	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTCGTTTTGAGCAGGTCTCACT	3+56<;?==?A?@AA@C@C?@A?=??@=>AB@DBBBB@@B@BC?B?>=>=>;@8?>?BA?BBB?>BA>8@@B@AA<@@@@&4??=:0:48D@>@??:%%%	BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIMKLJAAKNMPNPONOKJKHL	MD:Z:80T16	RG:Z:0	BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNQOPNHHPQPRQRPOPLNPLP	NM:i:1	AS:i:92	XS:i:20
HWI-ST142_0240:1:68:5688:125012#0	1024	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	3*57;:?==@>?@BB@?AA?@A>@????ABA>CABBBBAD@D@B?=@>>>ABBB@??B@?BAA@>BA>7A@B@B@@BB@A??@B:B@A??D@>@@?<;;=	BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIJKIJAAKNMPNPONOKJLLM	MD:Z:100	RG:Z:0	BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNNPNNHHPQPRQRPOPLNOPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:42:18843:165751#0	16	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	1<<A>=>DBCD@CCAAA?EBCDA?D@D@CBA@BCBCCA@A@CCC?DAA?@BBBD@?BE??BBB?BD?7@BB@@D@BBD@BE@BBBDAC??C??><92364	BD:Z:LNMNPONLONLKAKNLKOLKJKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMNNLNNMOOMNJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQQPPPPPRRQPIORQPRQONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHNNQLNHMLNMLNGHNPNOQPPOPPQROQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:47:9508:92109#0	1040	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	%;=A<??C@AD@CCB?A?E@CC@?C@C@CBA@BCCCCAAA@CCB?C???@BCCE@?BD??CCB?BE?7@BCA@E@CCD@?@>BBBD@B>@B>>=<;4353	BD:Z:LNMNPONLONLKAKNLKOLKJKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMNNLNNMOOMNJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQQPPPPPRRQPIORQPRQONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHNNQLNHMLNMLNGHNPNOQPPOPPQROQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:28:16767:127812#0	1040	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	.17><9=C@@868=7><@D7BB??B@B=A@A@?=7@A@?3@@@B?9==>@AAAB@>@B=@@@B>AD(6;@BA?B;@?B?AB@ABAD?@>?B<?><=3343	BD:Z:KMMMPONLONLKBJNLKNKJILLLJJJJJLMNKAAAJMIIKAILLLJIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJJMNMMMNNOOMNII	MD:Z:100	RG:Z:0	BI:Z:NPPOOPPORRQPHNRPPQQPOQPQNONONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPQQROPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:46:9810:69065#0	1040	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	1<<?=?>CAB>@CBA@B@DABC@>DAC?CCA?BBBBBA?@?BBB>C@@@ABBBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C??><92364	BD:Z:KMMMPONLONLKBJNLKNKJILLLJJJJJLMNKAAAJMIIKAILLLJIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJJMNMMMNNOOMNII	MD:Z:100	RG:Z:0	BI:Z:NPPOOPPORRQPHNRPPQQPOQPQNONONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPQQROPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:65:11459:169889#0	1040	chr3	58125489	60	100M	*	0	0	CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT	.9=>=>>CABD@BB?@A@D>BC@>B@C@BBA@BCBBB@?@?BBB?C@@>ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??B??><92364	BD:Z:KMMMPONLONLKBJNLKNKJILLLJJJJJLMNKAAAJMIIKAILLLJIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJJMNMMMNNOOMNII	MD:Z:100	RG:Z:0	BI:Z:NPPOOPPORRQPHNRPPQQPOQPQNONONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPQQROPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:21:7603:28973#0	0	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTCTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	35569?<=?A@@BC@B?BAAC??>?<=7<<@DBC@CC>C@D?AB??><>?AACA>?B@?D@>B=BA?7ACC@C@@BCAA@AACCCAA>?D@>A@C>;==<	BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJJJIJKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN	MD:Z:34T65	RG:Z:0.1	BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOOONOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:25:10397:24281#0	1024	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	44638?<??B?@BB@CAC?@C@A>@A?ACB@EBCCBBAD@DBBB>@>>>ACBC@A?C@@DCCB?C@@8@CC@C@ACC@@C@ACCC@A@@EA?AAC@<@=;	BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:46:19268:182386#0	1024	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	44638?<??B?@BB@CAC?@B@A?@A?ACBAEBCCBBAD@DCBB>@>>>ABCC@@?B@?ECCB?C@@7ACCBD@@CC@AC@ACCC@A@@DA>@AA@<>=;	BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:63:4260:118500#0	1024	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	4453:?<??B?@BB@CAC?AC?A??A??CB@DBCCBBAD@ECBB>A>>>ABCC?A?@@?CCCB?B@@7ACCAC@ADDAAC@ACCC@A?@D?>@@A=;>=<	BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:21:17428:130689#0	16	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	:=?;=>CBCEACC@AAAEBCCA?EBC@CCA@BCCCCA@AACCB?DAAAACBBD@?AD??BBB>BD?7@CB@@D@BCD?CD@BBBDAC??C@??><73684	BD:Z:NMNOOMMONMKAKNLKOLKJLLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMNLMNLNOPNMJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPROOORRQPIORQPRQPNPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHONPMNGNLNMLNGGNPNOQPPOPOQQQQQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:24:13055:69026#0	1040	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	88>9>>A@BD?B@A>@@D@<B=6B>B>6@A@ABBBBA@@@BB@?A>>=>BCCD@>BB?@BBB?AB=7<BBA@C>BBC>BB@AAAB?<<@A==><>:8363	BD:Z:MMMONNLOOLKBKNLKNKJILLLJJJJJLMNKAAAJMIIKAJKLLJJKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJIMNMLMNLONQNMII	MD:Z:100	RG:Z:0	BI:Z:PPOQNPNQRRPHNRPPQQPOQPQONOONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOQRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:44:19183:198872#0	1040	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	0485::=<?96;@<??;B>7??9=>>=@>A@;AAC@@?9=@2@??=<=><A9B@?@C>?>>7:?@?7<AB@@D>AB@<AC<AAAC?A>??=>?9=87473	BD:Z:MMMONNLOOLKBKNLKNKJILLLJJJJJLMNKAAAJMIIKAJKLLJJKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJIMNMLMNLONQNMII	MD:Z:100	RG:Z:0	BI:Z:PPOQNPNQRRPHNRPPQQPOQPQONOONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOQRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:5:16073:147208#0	1040	chr3	58125490	60	100M	*	0	0	GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG	79<:=>C@?C9?B<@>@CABC@?CBB@@AA@?BBCBB@>@BBB?C@>@ABBBD@>BD?@BBB>BC?7@BB@@D@BBB?BD@BBBCAC??A@??>=83574	BD:Z:MMMONNLOOLKBKNLKNKJILLLJJJJJLMNKAAAJMIIKAJKLLJJKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJIMNMLMNLONQNMII	MD:Z:100	RG:Z:0	BI:Z:PPOQNPNQRRPHNRPPQQPOQPQONOONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOQRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:3:12278:12956#0	0	chr3	58125491	60	100M	*	0	0	ATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGT	4623<:??A?@BB@CAC?AB@@?@@?<CB@EBCCCBADAEBCB>@>>>ABCD?@8B?@ECBB?C@@8ACCBDAACDA@D?ACCC@BB@EA>ABDA?A=;;	BD:Z:JJNNQOPLNNNKAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJKJKAAKNLONOONOMLNMMNJ	MD:Z:100	RG:Z:0.1	BI:Z:NNQQQQROPQQOHOPNOOPNOPPPOONOOPPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONOIIPRQSQRPQROMMQQQN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:43:2048:193931#0	16	chr3	58125491	60	100M	*	0	0	ATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAGATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGA	>?:<=DABF@CCA?A?EBDD@?BBEA@CAABBCCCA@A@CCC?DA??@C?CDA>BD??C@B?BD?7@CB@@E@BCD@BE@BBBDAC??C@@@?>;86854	BD:Z:MNONMLPNMLAKNLKOLKJLMKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMMJMKKLMMKJAJJNHJAIHJIHJAAIKJMMMLMMLMNPQMLJJ	MD:Z:59A39T0	RG:Z:0.1	BI:Z:QPRQNNQRQPIORQPRQPOPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQPOQPPQPPOOHOOPLOGMMNMLNGGMPNOQPPOPOPQPSQPNN	NM:i:2	AS:i:94	XS:i:20
HWI-ST115_0148:1:41:8424:161682#0	0	chr3	58125492	60	100M	*	0	0	TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA	3328:;=@?@CCAD@D@@CAA<?A?9@?;ABBCCC;D@CCCB?@>>=@BCC@A?B@@BCCB<BA?7ACCACAAC@?@B>ACDDA@?>D??@@CAAB>9;:	BD:Z:JJNNOQMNONKBJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJJKAAKNLONONNOLMNNMNJM	MD:Z:100	RG:Z:0.1	BI:Z:NNQORRPPQQOHOPNOPPNOPPPNPNNPPOQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNIIPRQSQRPQRPNNOQQNQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:68:19429:200488#0	1024	chr3	58125492	60	100M	*	0	0	TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA	42377<>A>ABB@CAC?AC@A??A??BA?DBCCCCADAEBCB?@>>>ABBC@??=@?BC>B?BA?7ACB?CAACB@AC@ACDD@A@?>?>A@B@?A>:<:	BD:Z:JJNNOQMNONKBJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJJKAAKNLONONNOLMNNMNJM	MD:Z:100	RG:Z:0.1	BI:Z:NNQORRPPQQOHOPNOPPNOPPPNPNNPPOQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNIIPRQSQRPQRPNNOQQNQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:42:11358:14661#0	16	chr3	58125492	60	15S85M	*	0	0	GTGCTCTTCCGATCTTGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGA	;?:;>?BA>7ABA@BD?@>C@BD@BB@?B?EABD@>CBC@BB@?BBBBB@?@?BBB?C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@AC>>;6854	BD:Z:HMOLLLLMNLNMLJIMOMLJMMKJAJNLKNKJILLLJJJJJLLNKAA@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHKAIILJJNDEIKII	MD:Z:85	RG:Z:0	BI:Z:LOQOMONQOPQQPPOPRQQOQQQPGNQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLPIINPMM	NM:i:0	AS:i:85	XS:i:19
HWI-ST142_0240:1:47:7919:158863#0	16	chr3	58125492	60	100M	*	0	0	TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA	?9::A@BD@BB@A@@D@BC@?CACBBCA@BCBBC@?@@BBB>C@@@A?BBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@??<;;8424	BD:Z:MOMLKOOMLBKOMKNKJILLLJJJJJLMNKAAAJMIIKAJLLKJJLMJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMMLLMLMLPQQILII	MD:Z:100	RG:Z:0	BI:Z:OQPPLPPQQINRPPQQPOQPQOOOOOPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPQRRLOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:16954:66540#0	1040	chr3	58125492	60	100M	*	0	0	TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA	:899?=@B>?>6?A?D@BB>>B>=>A9AA?=>,B?8675.A?A>==>7?@D@?@D48416;>D46@BB@@D>BBD@BD<B@?A=?=?A;?@?@<;;8324	BD:Z:MOMLKOOMLBKOMKNKJILLLJJJJJLMNKAAAJMIIKAJLLKJJLMJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMMLLMLMLPQQILII	MD:Z:100	RG:Z:0	BI:Z:OQPPLPPQQINRPPQQPOQPQOOOOOPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPQRRLOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:5:9086:50476#0	0	chr3	58125493	60	100M	*	0	0	GCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAG	43729<@>?AB@CAC?@B@@>@@@AA@?CABBBB@C@DB?B>@>>>BABB?@>B??BBCB?B@?7ABB@B@ABC?@B@AA@B?A@?DA>>>C@>B@::9;	BD:Z:IIMLQNOONKAKLJILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKLNNMHAKLLNNKLJAJJMIJAKIJKIJAAKMLOMONMNLLNNNNILM	MD:Z:100	RG:Z:0	BI:Z:MMNPRQRPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGPMNPNNHHPQPRPQPPRPOOPNPMPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:41:14541:111245#0	0	chr3	58125494	60	100M	*	0	0	CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT	47259>=?AB@CAC?@B@@???@>BB=CABBBBAD@DCBB?@>>>ABBBAA>C@?ECCB?BA?8ACCBC@ACCAAC@ACCC@A@@EA?ABDBACB><:;;	BD:Z:JJLNNPPOLBKLJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJAAKNLONONMNLLNONOJMNN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQQQRQOHOPNOPQOOPPPNONOOPPPNHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHPRQSQRPQRPOPQPONQQQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:43:1724:2639#0	1024	chr3	58125494	60	100M	*	0	0	CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT	3627<><?>B9B;C@=B?@>14<<597>9:8<=5?:AA:?:<<=899C-?>/?;5@6A?<B??7?24;<>;.@0::=?@?C@B??D??@@C>>7B%%%%%	BD:Z:JJLNNPPOLBKLJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJAAKNLONONMNLLNONOJMNN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQQQRQOHOPNOPQOOPPPNONOOPPPNHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHPRQSQRPQRPOPQPONQQQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:26:7434:174379#0	1024	chr3	58125494	60	100M	*	0	0	CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT	47249>=?AB@CAC?@B@@>>@>5A?>BAABBBAD@DBBB>@>>>ABBB?@>B??DBAB>B?@7@BCAC@@BC@@B?@BBB?@??D@?@@CA@DA=;9;9	BD:Z:IILMOOQNKBKLJJLLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKNNMI@KLLNNKLJAJJMIJAKIJKIJAAKMLOMNNMNKLNNOOILMN	MD:Z:100	RG:Z:0	BI:Z:MMPPQRRPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGONMPNNHHPQPRPQPPQPPPPPNMPPQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:44:6770:103256#0	1024	chr3	58125494	60	100M	*	0	0	CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT	4624;>=?@A?A<A?@B@???@??BB@DBBBBB@D@DBBB?@>>>AABB?@?A??CABA?B@?7@B?>C?@BB?@B??BB@?A@?D@>@@C@@DA=;9:7	BD:Z:IILMOOQNKBKLJJLLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKNNMI@KLLNNKLJAJJMIJAKIJKIJAAKMLOMNNMNKLNNOOILMN	MD:Z:100	RG:Z:0	BI:Z:MMPPQRRPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGONMPNNHHPQPRPQPPQPPPPPNMPPQ	NM:i:0	AS:i:100	XS:i:20
HWI-ST142_0240:1:43:4359:117904#0	16	chr3	58125494	60	100M	*	0	0	CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGA	6(;:>C@BB@@A@C@BC@?=AC@BB@@CBCBB@@@?BCB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@<>@@92754	BD:Z:MLJMNMLCLOMLOKJILLLJJJJJLMNKAAAJMIIKAJLLLJILMKAHHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKLMKLLNOPLPLJII	MD:Z:99T0	RG:Z:0	BI:Z:PPNPNPOHOSPPQQPOQPQOOOPOPQPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPRNQPNMM	NM:i:1	AS:i:99	XS:i:20
HWI-ST142_0240:1:48:9513:187557#0	1040	chr3	58125494	60	100M	*	0	0	CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT	99=;@B@BB?@@@C@BC@>C@CABCA@BBBCB@@@?BCB>B@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@=>@@92744	BD:Z:MLJMNMLCLOMLOKJILLLJJJJJLMNKAAAJMIIKAJLLLJILMKAHHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKLMKLLNOPLPLLII	MD:Z:100	RG:Z:0	BI:Z:PPNPNPOHOSPPQQPOQPQOOOPOPQPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPRNQPPMM	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:44:1737:13754#0	0	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCGGGTCTCCATGTAGTG	3244<;>AA@DAC?@B>?>AA>ACBAEBCCBBAE@DBCB>@>>>ABBB?A?B?@DBBB?BA@8ACCBD@ACC@A@A@CCC@AB>8>>@6D@?DB>>;9<;	BD:Z:JJNKPQPLCKLKJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@AKNLOLMJMNKLNNOOKMNNI	MD:Z:84A15	RG:Z:0.1	BI:Z:NNQORRROHOPNOPQOPPPPNONNPPOQNGHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHORQSOPKQRPOPRQPLQQQM	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:44:17666:60002#0	1024	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	4245;;>AA@CAC?@BA?>@A??CB@EBCCBBAD@DBCB>@>>>@BBB?@?B@@DCCC>C@?8ACCBC@@CC?AC@ACCC@AB@FA?A@D@ADBA?;;<<	BD:Z:JJNKPQPLCKLKJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@AKNLONONMNKLNNOOKMNNI	MD:Z:100	RG:Z:0.1	BI:Z:NNQORRROHOPNOPQOPPPPNONNPPOQNGHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHORQSQRPQRPOPRQPLQQQM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:67:11815:169115#0	1024	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	3226<<>A<?B?B@AB>>?@>9;>9=C@>>@C@;?DCCB>?<<<A??<=>?B@?C@AA<B@?6A@B@CAA;@A>=?>A?<@@??D@=@@C@>EB@%%%%%	BD:Z:JJNKPQPLCKLKJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@AKNLONONMNKLNNOOKMNNI	MD:Z:100	RG:Z:0.1	BI:Z:NNQORRROHOPNOPQOPPPPNONNPPOQNGHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHORQSQRPQRPOPRQPLQQQM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:45:19953:130237#0	1024	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	4244;;>AA@CAC?@B@@>@@>=A@@DBBBBBAD@DBBB>@>>>BBBB?@>B??DCBB?B??8@BBBC?@CC@AB@@BBB?A@?D@>AAAA@DB>>9:;:	BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI	MD:Z:100	RG:Z:0	BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:63:9832:33189#0	1024	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	4244;;>AA@CAC?@B@@>@>>9??=BAAABB@D@DBBB>@>>>ABBB?@>B??DBBA>B??7@BB@C?@BB@@B@@BBB?A@?D@>A@CA@DB>>9;:9	BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI	MD:Z:100	RG:Z:0	BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:67:3073:131364#0	1024	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	4234;<>AA@CAC?@B@@>@?=:?8?BABBBBAD@DBBB>@=>>BBBB?@>B??BACB>B??7ABBBB?@BB?@B@@BBB;@@?D@>A@C@@D@>>::;9	BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI	MD:Z:100	RG:Z:0	BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:6:19159:38857#0	1024	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	4244;;>AA@CAC?@B@@>@@@@BBADBBBBBAD@DBCB>@>>>@BBB?@>B?<DBBB>B??7@BBBC?ABB?AB?@BBB=@@?D@>AAAA@DB>=9;;:	BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI	MD:Z:100	RG:Z:0	BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:21:4682:56360#0	16	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTA	9><>CABCAAA?EBCDA?EBDBCCAABCCBCA@@ACCC?DAAA@CCCE@?BD??CBB?BD?7@BB@@D@BBD@BD@BCBDAC??C@@@@@>>AB<77424	BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLMJJ	MD:Z:99G0	RG:Z:0.1	BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPPNN	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:27:11042:76831#0	1040	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	:>9>DACC>@@@E@[email protected]@D@@CA@BB@BBAA@@CC@?CA>?@BCCE@?BD??BCB?BE?7@BB@@E@BBD@>D@BBBD@C?>CAA@>@>@@B<97484	BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLIJJ	MD:Z:100	RG:Z:0.1	BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPMNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:64:15606:175538#0	1040	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG	:?;>D@CB@AA?EBCCA?EBDBCCA@CCCCCA@@@CCC?DAA?BCCCE@?BD??BBB>BD?7@BB@@E@BBD@BD@BBBDAC??C@@@@@>>AB<77484	BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLIJJ	MD:Z:100	RG:Z:0.1	BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPMNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:65:18444:50475#0	1040	chr3	58125495	60	100M	*	0	0	TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTA	:=<>DABCA@A?EBCDA?DBD@CBBABCBCCA@@ACCC?DAAABCCCE@?BD??BBB>BD?7@BB@@D@CBD@@D@BBBDAC??C@@@@@>>AB<77424	BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLMJJ	MD:Z:99G0	RG:Z:0.1	BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPPNN	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:24:8906:58142#0	0	chr3	58125496	60	100M	*	0	0	AGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG	34468<@A?CAC?AB@@>@A?AAA>EBCCCCAD@EBBB>?>>>@BCCAA>B??ECCB?CA>8ACC@CAACCAAB@@CCC@A??D@?ABCB?EC?A=<;;8	BD:Z:JJKMQQMCLLKKMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@KNLONONMNKKNNNPKNNNIM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPSRPHOPNOPQOPQPPNONNOQOPOGGHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQQSQRPQRPOPRRQMOQQMO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:7:12620:40059#0	1024	chr3	58125496	60	100M	*	0	0	TGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGA	43468<@A?CAC?@B@@>@@?ACB@EBCCCBAD@DBBB?@>>>ABBB?@?B?@ECCC?C@@8@CCBD@@CC@AC@@CCC@A@>E@>ABDBAEC?@>;;<<	BD:Z:JJNMQQMCLLKKMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@KNLONONMNKKNNNPKNNNIN	MD:Z:0A98G0	RG:Z:0.1	BI:Z:NNQPSRPHOPNOPQOPQPPNONNOQOPOGGHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQQSQRPQRPOPRRQMOQQMQ	NM:i:2	AS:i:98	XS:i:19
HWI-ST142_0240:1:42:11406:175267#0	1024	chr3	58125496	60	100M	*	0	0	TGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG	43468<@A?CAC?@A@@>@@>=A>=DBBBBBAD@DBBB>@>>>A?BB?@>B??DBBB>B?>7@BBAD?@CC@AC?ABBB@>@?D@>@BCA@CB??<;;:5	BD:Z:IIMLRPMBLMJJMMIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMMIAKKLNNKLJAJJMIJAKIJKIJAAKMLOMNMLNKKMNOPKMMNIM	MD:Z:0A99	RG:Z:0	BI:Z:MMPPRRPGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMPMNHHPQPRPQPPQOOQRQPMNPQMO	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:64:3826:31604#0	1024	chr3	58125496	60	100M	*	0	0	AGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGCGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG	4336:<?A>A?C?@@@=?@A=:@<=CAA?AB?D@DBB@?@=(3;=@@@?>A??DBCB?B@?8@@A?CA?@B@AB@@BBB?>@?D?>@@1<=B@>?<:::5	BD:Z:IIKLRPMBLMJJMMIIMKLJKJIJLJLI@@@IJGHH@JKLLLJLKI@JHKKMMMIAKKLNNKLJAJJMIJAKIJKIJAAKMLOMNMLNKKMNOPKMMNIM	MD:Z:41G58	RG:Z:0	BI:Z:MMOPRRPGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOOMPPNGOMPPPPONGPOPPPMOMGONPMMGOMMPMNHHPQPRPQPPQOOQRQPMNPQMO	NM:i:1	AS:i:95	XS:i:20
HWI-ST115_0148:1:24:12153:162085#0	0	chr3	58125497	60	100M	*	0	0	GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGCTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA	3556=?@>??@??C?=;:<;2>95=;=@=:?C@DCCB?@=?>=BB?@=:=A?DCCB?BA?7>=C@C@?AC@@@@>=?B>?9=B?<B5B<=?@@@>>=;%%	BD:Z:JJMNQNDLMKKNMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMMMIJIJJIJ@@JNLONONMNKKMNNOLNONIML	MD:Z:67T32	RG:Z:0.1	BI:Z:NNPQSPIOPNOPQOPQQPNONNOPPPNHGGOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPQONONNONNHHOQPSQRPQRPOPRRRNPOQMOP	NM:i:1	AS:i:95	XS:i:20
HWI-ST115_0148:1:24:7479:25230#0	16	chr3	58125497	60	100M	*	0	0	GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA	:<@;AC3?B?D@CC@@CBC@B@?@=A3@A@B;@CBB?A<=AABCCE@?CE?@ACB?BD=8>CCA?C@CBDACB@CCCB@@??B>?<?A=@=B><?::233	BD:Z:NMLKBLPMLPLKJLMLKKKKKLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKMNMJNNOLMMJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPOFMQQPRQPOQQQPOPOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMNMMNGHMOMNPOOOPOPPOQQMPQQOQONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:27:11695:17911#0	1040	chr3	58125497	60	100M	*	0	0	GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA	:=@=BB??A@EBCDA@D@C@CCA@BCCCCA@AACCC?CAAABCCCEA?BE??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BC?<?98454	BD:Z:NMLKBLPMLPLKJLMLKKKKKLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKMNMJNNOLMMJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPOFMQQPRQPOQQQPOPOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMNMMNGHMOMNPOOOPOPPOQQMPQQOQONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:46:6962:183922#0	1040	chr3	58125497	60	100M	*	0	0	GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA	;=@<BCAAAAE@CDA?DBC@CBA@BCCCCA@@ACCB?DAAA@BCCDA?BE??BBB>BD?7@BB@@E@BBD@BD@BBBDAC??C@@@@@>?BC?<?98454	BD:Z:NMLKBLPMLPLKJLMLKKKKKLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKMNMJNNOLMMJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPOFMQQPRQPOQQQPOPOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMNMMNGHMOMNPOOOPOPPOQQMPQQOQONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:48:17649:64283#0	0	chr3	58125498	60	100M	*	0	0	ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG	3536<>=?<A=@A6?<;>:>;76:1737+3C<BCC>552<;7>>@@;>?=>BA<@?=@?4@?@?B@<9>?::59:@B@A?7D?;@@A>>BA@@?>><7%%	BD:Z:IINMNDNMKKMMJJMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLHAKLLMNKLJAJJMIJAKIJKIJAAKMLOMNMLMJKMMNPKNOOIMKL	MD:Z:100	RG:Z:0	BI:Z:MMPPPIPPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMNGHPQPRPQPPQOOPQRRNPPOMOPO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:65:18859:157756#0	1024	chr3	58125498	60	100M	*	0	0	ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG	3539<=<A>A=AB5====6=<<9<;??AA;C?CBBB?@=<?=B?B@@??A?DB@B<?6>52;;5=AABB@;6><@??=@?<D@>A@@:>?AA>=?%%%%%	BD:Z:IINMNDNMKKMMJJMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLHAKLLMNKLJAJJMIJAKIJKIJAAKMLOMNMLMJKMMNPKNOOIMKL	MD:Z:100	RG:Z:0	BI:Z:MMPPPIPPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMNGHPQPRPQPPQOOPQRRNPPOMOPO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:3:13191:78850#0	16	chr3	58125498	60	100M	*	0	0	ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG	>@;?B?@@@DBCCA?CBEBCCA@CC@CCAAA@BCA?D??ABCCCDA?CD@?CCB?BD?7@CCAAC@BBD@AD@BBBDAC??CA@@@A=?BD?=A<=4574	BD:Z:MLKALONLPMKJLMLKKKKKMMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLNMINMNLPMKJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPOHLPPPRQPOQQQPOPPOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMOMLOGGNOMNPOONPOPPOQQMPPQNSONNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:7:9375:185965#0	1040	chr3	58125498	60	100M	*	0	0	ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG	=?<=@@@@@D@BC@?C@C@BB@?BCBCCA@@?BBB>CA@>ABBBD@>BD?@BBB=BD?7@BBA@D@BBD@BD@BBBDAC??C@@@@@>?BD?>A<>4574	BD:Z:MKJAKPNMPLKJMLLJJJJJLMNKAAAJMIIKAJLLLJJLMK@IILLLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKKMNMJMMOKPLJII	MD:Z:100	RG:Z:0	BI:Z:PPOGKQOPRRPOQPQOOOPOQQQQGHGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPRNRONMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:22:8056:180239#0	0	chr3	58125499	60	100M	*	0	0	TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG	4346;<A@B?@BAA>A@AACB@DBCBBBAD@DBCB?@>>>ABBCA@?B??ECCB?C@@8ACCBD@@CC@AC@@CCC@A@@EA?A@CBAEC?@?@A@:<:9	BD:Z:JJNKENNLLNNKJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKONONMNKKMMMOKNPOJMLLN	MD:Z:100	RG:Z:0.1	BI:Z:NNQOJPQNOPQOPQQQOONNOPOPOGGHNOMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPRPQRPOPRRRORQPKOPPO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:26:13604:44997#0	1024	chr3	58125499	60	100M	*	0	0	TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG	4346=>A@B??CA@?>@>?CA?BABBBBAD@DBCB?A>>>BACD?A?B@@DACC?CA@8ACCBC@ABC?AC?ACCC@AB@FA>ABDBAECAA???>;<:8	BD:Z:JJNKENNLLNNKJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKONONMNKKMMMOKNPOJMLLN	MD:Z:100	RG:Z:0.1	BI:Z:NNQOJPQNOPQOPQQQOONNOPOPOGGHNOMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPRPQRPOPRRRORQPKOPPO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:25:6242:39043#0	1024	chr3	58125499	60	100M	*	0	0	TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG	4346;<A@B?@B@@>>@>:AA@CAABBBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBBC?@CB?AC@ACBC?@@?D@>@ABA>DB?@?@?=9;;9	BD:Z:IIMJENOKKNMJJNKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@KLLNMKLJAJJMIJAKIJKIJAAKMLOMNMLMJJMMNOKNOPJMKLM	MD:Z:100	RG:Z:0	BI:Z:MMPNIPRNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMHGPQPRPQPPQOOPQQROQPQKOPOO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:6:7030:191659#0	1024	chr3	58125499	60	100M	*	0	0	TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG	2347<;>>:?@A>;;<?=6??;A<>AAB@C=CBBB?>>>=A@BB@@?BA?CBCB>>@>6=@@>A?@B@@AB@AAB@?@??A?>@@B@>CB@@>@?=::85	BD:Z:IIMJENOKKNMJJNKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@KLLNMKLJAJJMIJAKIJKIJAAKMLOMNMLMJJMMNOKNOPJMKLM	MD:Z:100	RG:Z:0	BI:Z:MMPNIPRNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMHGPQPRPQPPQOOPQQROQPQKOPOO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:61:19340:26239#0	16	chr3	58125499	60	3S97M	*	0	0	ATCTGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG	;;9?;;84;<>C@BC@?E?E?AA@@ACA<C@??<;@<?C>><@BCAC<?BE?AC?B>BE=5>CC??C>ACD@<C@CAAE@B>?B>?A?==?AB=;>4613	BD:Z:MKOLLBMOMLOLKJLMLKKKKJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKLMNNJOOOIMJJ	MD:Z:97	RG:Z:0.1	BI:Z:POQPMGNRQPRQPOQQQPOOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNLNNLNHGMOMNPOPOPOPPOQQMQQRMQNN	NM:i:0	AS:i:97	XS:i:20
HWI-ST142_0240:1:22:5430:189914#0	16	chr3	58125499	60	100M	*	0	0	TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG	?<===?A@D@BCA>CABABB@?BBCBC@@@@BBC>C@A@ABBBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@=B>@95744	BD:Z:KJAJONMPMKJMMLJJJJJLMNKAAAJMIIKAJLLLJJLMKAHILLMLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJMNMIMMMKOPJLII	MD:Z:100	RG:Z:0	BI:Z:POGMOOOQRQOQPQOOOPOQQQQHGHNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPNRQNPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:25:7867:50214#0	16	chr3	58125500	60	100M	*	0	0	GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT	;>>=@A?DBCCA?E@DBCCA@CCCCCA@A@CBC?DAA?BBCCEA?CE??CBB>BD?7@BB@@D@CBD@BD@BBBDAC??C@@@@@>?BD@>B?B<:7444	BD:Z:KAKNMLQMLKLMLKKKKKMNNKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLIMLMJOPNLMJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHNQNNQQPOQQQPOPPPQQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMNGHMONNPOONONPPOQQMPPPMRPPPONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:6:13768:9775#0	1040	chr3	58125500	60	100M	*	0	0	GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT	;><>@A@EBCDA?CBE?CCA@CCCCCA@AACCB?DA?A@CCCEA?CE@?CBB?BD?7@CB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>B?B<:7444	BD:Z:KAKNMLQMLKLMLKKKKKMNNKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLIMLMJOPNLMJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHNQNNQQPOQQQPOPPPQQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMNGHMONNPOONONPPOQQMPPPMRPPPONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:24:7977:30681#0	1040	chr3	58125500	60	100M	*	0	0	GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT	79:<<?<@>;=@?B@B@BA>@ACAB7A@@@896><>>>@ABBC@?BD>??AB?BD>7@BB?9A8=@D?AB?BBBA@?>@B>;@?@>?<A<><<A::7123	BD:Z:JAJNMMPMLJMMMJJJJJLMNKAAAJMIIKAJLLLJJLMKAIHLLMMMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLNMILMMIOONLLII	MD:Z:100	RG:Z:0	BI:Z:OGMQMOPQQPQPQOOOPOQQQQHHGOPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLRQPPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:67:7724:174315#0	1040	chr3	58125500	60	100M	*	0	0	GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT	6:::<@@D@BC@?C@B@BB>?@CBBAB@@@BBA?A>>>@AABD@?AC?@BBA>AA=5@BBA>C?BBD@BC@ABBD@A??A>@@?@=?AD?>B?A::8134	BD:Z:JAJNMMPMLJMMMJJJJJLMNKAAAJMIIKAJLLLJJLMKAIHLLMMMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLNMILMMIOONLLII	MD:Z:100	RG:Z:0	BI:Z:OGMQMOPQQPQPQOOOPOQQQQHHGOPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLRQPPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:64:14210:42818#0	0	chr3	58125501	60	100M	*	0	0	AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG	4664<=A>?B@@>@@>?BA@DBCCCB@D@ECBB>@>>>ABBBA@>B??BBBB?BA?8@ACBC?ACCAAC@ABC@@A@?D?>@@C@?EB?A??A?><<%%%	BD:Z:JJBKONMOOKKNLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMONMNKKMMMNJNOOKNMLNMI	MD:Z:100	RG:Z:0.1	BI:Z:NNHOROPPQOPQQQOPONOPOPNGHGNPLLNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOQRPOPRRRORRRMNNPOPM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:68:20373:148127#0	1024	chr3	58125501	60	100M	*	0	0	AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG	4664<=A>?B@@>@@>>BA@DBCCCB@D@DCCB?A>>>ABBC?@?B??BCCB?B@?8@CC@D?A<BAAA@AC-C@>?>??>A@C@>=BA@?B@?<@<78;	BD:Z:JJBKONMOOKKNLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMONMNKKMMMNJNOOKNMLNMI	MD:Z:100	RG:Z:0.1	BI:Z:NNHOROPPQOPQQQOPONOPOPNGHGNPLLNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOQRPOPRRRORRRMNNPOPM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:45:11826:198924#0	1024	chr3	58125501	60	100M	*	0	0	AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG	4664<=A>?B@@>@@??BA?DBBBBBAD@DBBB>@>>>@BBB@A?B??DBBB>B@?7@BBAC@@BB@@B@@BAC@A@?D@>@ACA@DA?@?@A@>?:8*8	BD:Z:IIAKPMMNNKJNLMJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKLNNKKJAJJMIJAKIJKIJAAKMLOMNMLMJJLLNOJMOPKOLLMLI	MD:Z:100	RG:Z:0	BI:Z:MMGNRPPPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGPPPRPQPPQOOPQQQNRRRMONOOOM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:45:5304:83561#0	1024	chr3	58125501	60	100M	*	0	0	AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG	4664<=A>?B@@>@@@ABB@DBBBBBAA@DBBB?@>>>ABBB?@>B??CBBB?B@?7@BB@B@@BB@AB@ABBB@A@?D@>@ACA@DC?@>@@?=?;8*9	BD:Z:IIAKPMMNNKJNLMJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKLNNKKJAJJMIJAKIJKIJAAKMLOMNMLMJJLLNOJMOPKOLLMLI	MD:Z:100	RG:Z:0	BI:Z:MMGNRPPPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGPPPRPQPPQOOPQQQNRRRMONOOOM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:1:7700:156580#0	16	chr3	58125501	60	100M	*	0	0	AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG	;<9;??DABC@>D@C@BBA?BBBBC@?@@CCB>D@@@B@BBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C?C>=<4484	BD:Z:AJNLLPMLKMMMKJJJJLMNKAAAJMIIKAJLLLJJLMKAIIKLMMNJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMMILLMIMOMPLHII	MD:Z:100	RG:Z:0	BI:Z:GMQOMPPPPRPQOOOPOQQQQHHHNQMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPQPROLMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:22:11100:183122#0	1040	chr3	58125501	60	100M	*	0	0	AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG	5.:;??D@BA>=C>C>B?@5@BBBB>;8>4B@?B>8>@?@CD@=BB:?<<A?AC=7@BBA?C=BBA@BA?BABC?A>?A==6=A<@<C>>A>B;<<3373	BD:Z:AJNLLPMLKMMMKJJJJLMNKAAAJMIIKAJLLLJJLMKAIIKLMMNJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMMILLMIMOMPLHII	MD:Z:100	RG:Z:0	BI:Z:GMQOMPPPPRPQOOOPOQQQQHHHNQMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPQPROLMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:43:19528:142803#0	16	chr3	58125502	60	100M	*	0	0	AACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGA	=<;=@DACCA?D@D@CBA@BCCCCA@A@CCC?DAA?@CCCEA?CD??CBC>BD?7@BB@@D@BBD@BD@CBBEAC??C@@A@@>?BE@>C@C???94854	BD:Z:KNLKPMMKMNLKKKKKMNNLBAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLLLINNMOPILJJ	MD:Z:100	RG:Z:0.1	BI:Z:NQPOOOOOQQQPOPPPQRRPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHGMPMNQOONONOOOQQMPPPMQOOQQMPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:21:20040:121958#0	1040	chr3	58125502	60	100M	*	0	0	AACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGA	869==CA@C@>CBB@@BA?BBBBBA@@?BBC>C@@@ACBBD@?BD??B@B>BD?8@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@C???94854	BD:Z:JNLKOMLKNMMKKJJJLMNKAAAJMIIKAJLLLJJLMKAIILKMMNK@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLILLLIMMMOPHKII	MD:Z:100	RG:Z:0	BI:Z:MQOONPOORQQOOOPOQQQQHHHOPNMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPOPRQLPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:8:13725:143226#0	1040	chr3	58125502	60	100M	*	0	0	AACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGA	%%%%%%%%A=50>C@BB@@9BBBBA@A@BBA?>@>?>BBBD@?BD??BBB?BD?8@BBA@C@ABD@AD@BBBD@@=?C@@A@@>?BC;?B?C?>?94734	BD:Z:JNLKOMLKNMMKKJJJLMNKAAAJMIIKAJLLLJJLMKAIILKMMNK@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLILLLIMMMOPHKII	MD:Z:100	RG:Z:0	BI:Z:MQOONPOORQQOOOPOQQQQHHHOPNMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPOPRQLPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:46:2641:21330#0	0	chr3	58125503	60	100M	*	0	0	ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC	4475<;?A?@>?@@?BA@DBCBCBAD@ECCB>A>>>ABBB?@>B??DCCB?B@@7ACC@C@ACC?ACAABCC@B?=E@?A@D@AEC@@?AA@?@?%%%%%	BD:Z:JJLKNQPLLNMNJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMMNKKMMMNJMNOJNNMOMINM	MD:Z:100	RG:Z:0.1	BI:Z:NNPNQQROPQQQOPOOPPOPNGGGOOLMMGPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQPOPRRRORRRNPPOMPMQP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:25:12209:32246#0	1024	chr3	58125503	60	100M	*	0	0	ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC	4365<;>A?>=?@@?A>=@=ABBB@D@CBBB??>7>?ABB???A??=BBB?B??8ABB@B@@BB@@B@ABBB?B??D@>@>C@@DBA??@@?>A?<2:56	BD:Z:IILJNPPKKOLMKLJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMNKLJ@JJMIJAKIJKIJAAKMLOMNMLMJJLLMNJMNOKOMNNLIMM	MD:Z:100	RG:Z:0	BI:Z:MMPNPRRNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPPQPQPPQOOPQQQNQQSOPPOMOMPP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:48:11161:156653#0	1024	chr3	58125503	60	100M	*	0	0	ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC	4475<;>A??>?@?>BB?DBBBBB@D@DBBB?@>>>ABBB?@>B??CBBB>B??7@BBAC?@CB?@C?@CBC@A@?D@>@ACA@CB?@=@@?>@?<<986	BD:Z:IILJNPPKKOLMKLJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMNKLJ@JJMIJAKIJKIJAAKMLOMNMLMJJLLMNJMNOKOMNNLIMM	MD:Z:100	RG:Z:0	BI:Z:MMPNPRRNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPPQPQPPQOOPQQQNQQSOPPOMOMPP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:5:13378:186738#0	1024	chr3	58125503	60	100M	*	0	0	TCTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC	3256<:9?==<<@:=@;=@????8=98>?6<9<=<2,364347/==A7@@?A?>7==?=??<2;=948?@BB@A>:@><82A?<@BA?=%%%%%%%%%%%	BD:Z:IIJJNPPKKOLMKLJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMNKLJ@JJMIJAKIJKIJAAKMLOMNMLMJJLLMNJMNOKOMNNLIMM	MD:Z:0A99	RG:Z:0	BI:Z:MMNNPRRNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPPQPQPPQOOPQQQNQQSOPPOMOMPP	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:25:4411:84732#0	16	chr3	58125503	60	100M	*	0	0	ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC	<;;=DACCA?DBCBCCA@BCCCDA@B@CCB?DAAABCCCEA?CE@@CCB?BD?7@BB@@D@BCE@CD@CBBDAC??C@@@@@>?BD@>C@D?@A<99544	BD:Z:NLKOMLLMNMKKKKKMNNLBBAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKLIMNLNPLLNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPOQNNNQQQPOPPPQRRQHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHMONNPPONONOONQQMPPPMQONQPOPQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:48:8985:65471#0	1040	chr3	58125503	60	100M	*	0	0	ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAA	%%97A:@94>74?.-?A6@-B@7A?A8B6B?C>>>??@BB=>4>::@@?>?@93*<A=3B8ACC<AB@BBBA=@89?:=@0(,8-@5;6<@=>?9::353	BD:Z:NLKNLLKNNMKKKJJLMNKAAAJMIIKAJLLLJJLMKAIILLLMNKAIKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHLLLHMMKOOLKJII	MD:Z:99C0	RG:Z:0	BI:Z:QOOPNONQQROOOPOQQQQHHHOQMNOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONRQNPOMM	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:5:9908:10539#0	1040	chr3	58125503	60	100M	*	0	0	ACTCTGAAGTAGAGAATCGTTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC	7;;;@@BC@>CAA@BB@7@@ABB@??@BBA?B@>?@BABD@>BD?@B@B?BD?8@BBA@D@BBD@BD@BBBAAB??A@@@@@>?BD@>C@C?@A<99344	BD:Z:NLKNLLKNNMKKKJJLMKKAAAJMIIKAJLLLJJLMKAIILLLMNKAIKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHLLLHMMKOOLKMII	MD:Z:18A81	RG:Z:0	BI:Z:QOOPNONQQROOOPOQQOOHHHOQMNOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONRQNPPMM	NM:i:1	AS:i:95	XS:i:20
HWI-ST142_0240:1:48:10959:184359#0	0	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	3538:<?=>=<>97@A;AAAAA>,C;;A=;><===?@2@:=7?>>7<??9B?>5ABB@@??;@?@:>@@@/8A6<B==@?0>=B@@??=<@;@@;>;;%%	BD:Z:IIJJQPMKOMMKLKIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMKLJAIJMIJAKIJKIJAAKMLOMNMLMJJLLMNIMNOJOMNOMIMMI	MD:Z:100	RG:Z:0	BI:Z:MMNNRRPOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOROQPPQOOPQQQNQQROQQOOMMPPL	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:68:13457:188269#0	1024	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAATTATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACA	3638:=?=?==<<<>./3=166*%85266.3466/?;:4:41161BB/@6?5,6=9@<A;@%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:IIJJQPMKOMMKLKIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLHJIIKMMKLJAIJMIJAKIJKIJAAKMLOMNMLMJJLLMNIMNOJOMNOKJKMI	MD:Z:47A47T4	RG:Z:0	BI:Z:MMNNRRPOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONPNPPPPMOMGONPMMGOMMOMMGGOPOROQPPQOOPQQQNQQROQQOOHJPPL	NM:i:2	AS:i:90	XS:i:20
HWI-ST142_0240:1:7:15934:30090#0	1024	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	47578<@??>?@@=BB@DBBBBBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BB@C?@@B?@B@@BCC@A@?D@>@@CA@DB?@??@??A@=>;;7:	BD:Z:IIJJQPMKOMMKLKIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMKLJAIJMIJAKIJKIJAAKMLOMNMLMJJLLMNIMNOJOMNOMIMMI	MD:Z:100	RG:Z:0	BI:Z:MMNNRRPOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOROQPPQOOPQQQNQQROQQOOMMPPL	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:45:11810:80190#0	16	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	;:;??AC@>CAA@BB@?BBBCB@?@?BBB?D@@AABBBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@>>?BD@>C@D@@B><=5434	BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII	MD:Z:100	RG:Z:0	BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:4:11059:91320#0	1040	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	9;:??AC??CAB@BB@?BBBBB@?@?BCC?D@>A@BBCD@>BD??BBB>BD?8@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@@B><=5434	BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII	MD:Z:100	RG:Z:0	BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:66:2424:23337#0	1040	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	;::??AA@?C@C@BBB?BBBBB@???CCC?D@>AABBBD@>BD??BBA>BD?7@BB@@D@BBD@BD@BBBD@C?@C@@@@@=?BD@>C@D@@B><=5234	BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII	MD:Z:100	RG:Z:0	BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:6:16257:60848#0	1040	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	9:8??AA@?C@C@BBA?BBBABA@@?BB@?B>>4@=BCD@?BD??BBB?BD?8@BB@@D?CBD@BC@BBBD@<??B@@@?=>?AD@?C@C@>B><=5434	BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII	MD:Z:100	RG:Z:0	BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:7:7721:186507#0	1040	chr3	58125504	60	100M	*	0	0	CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA	;::??AC@>CACBBB@?BBBBB@?A?CCC?D@>A@BBCD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@A>?BD@>C@D@@B><=5434	BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII	MD:Z:100	RG:Z:0	BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:41:11840:25955#0	0	chr3	58125505	60	100M	*	0	0	TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACAT	45739>>?=@@AACBAEB==BBAD@DBBB>@>>>ABBB?@>B??DCBB>BA?8ACCBC@@CC@AC@ACCC@AA@E@?ABCBBEC@A?AA@>B@?%%%%%%	BD:Z:JJKNQNMONNKLJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMKKMMMNJMNNINMMPKKLMIM	MD:Z:94T5	RG:Z:0.1	BI:Z:NNOPSPQQQQOPOOPQPPNGGGNOMLMHOOQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONPRRRORRRNPQQOIHPPMQ	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:42:14945:88827#0	16	chr3	58125505	60	100M	*	0	0	TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAA	<9>;BD@?CAD>CCA@BCCCCAAA@CCC?CA?ABBBCCA?CE?@BCB?@D?7@CC@@D@BCD@CE@BBBE@C?@C?@A@@>@BD@>C?D@AB?>@:5364	BD:Z:KOLKKMOMLLKKKMNNLBBBKMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHMMKMNKOQJKJJ	MD:Z:99T0	RG:Z:0.1	BI:Z:OQPOLOPQPOPPPQRRQIIHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPMNQOOOONOONPPMPPPMQONPONQSNONN	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:43:3535:86981#0	1040	chr3	58125505	60	100M	*	0	0	TATGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAT	7-<:;?868959):=:?AA(@A?7?BA@?C><;?BBBD??BD>?B?B?BD?8@BC@@D:?CB:@@<?CBA<C?>A@@@?@=?AB?>=>B=>=99?;6353	BD:Z:LMKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKNQINII	MD:Z:1C98	RG:Z:0	BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONRRMPMM	NM:i:1	AS:i:98	XS:i:0
HWI-ST142_0240:1:4:15304:147199#0	1040	chr3	58125505	60	100M	*	0	0	TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAT	8:?<@B@?CAC@BBA?BBBBB@?@@BBB?DA@@@BBBE@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@AB?>@:4364	BD:Z:KNKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKNQINII	MD:Z:100	RG:Z:0	BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONRRMPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:62:21314:22111#0	1040	chr3	58125505	60	100M	*	0	0	TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGNCAT	88=;?B9?B@;@BB@@BCB8BA?@?BB@?C>?=?BBBD@?BD??BBB?BD?7@BB@@D@BAC?BB@BBBD@C?@A@@@@@>?BD@?C@D?=@;6>:%432	BD:Z:KNKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKLMINII	MD:Z:96A3	RG:Z:0	BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONOOMPMM	NM:i:1	AS:i:98	XS:i:19
HWI-ST142_0240:1:68:17222:120327#0	1040	chr3	58125505	60	100M	*	0	0	TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAT	9;?<@B@>BAC@B@@@A@B=BA@@@;A@?A>@>@BBBE?>BD?ABBB>BD?7@BBA@D?ABD@BC@BBBD@B??B<@@@>>?AC@<C@D@AB?>@:4354	BD:Z:KNKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKNQINII	MD:Z:100	RG:Z:0	BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONRRMPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:23:6412:139348#0	0	chr3	58125506	60	100M	*	0	0	CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGCGCCATA	3637<;><7@17/0-34.>??88?A@>>><=>?7B>;:7;;:979>>.=7:4=?<=<@A35<?<<>B4:=-815>0A>@=>B=A%%%%%%%%%%%%%%%%	BD:Z:JJNNNNPNOKLKJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJKMMMNJMNNIMMMOPMONMML	MD:Z:93T1A4	RG:Z:0.1	BI:Z:NNPQQQRQQOPOOPQPQNGGGNOLMMGPOPOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONORRRORRRNPQQPOMNPQQP	NM:i:2	AS:i:93	XS:i:19
HWI-ST115_0148:1:28:17969:176762#0	1024	chr3	58125506	60	100M	*	0	0	CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATA	3735<>=>@@>;>?>BABBAC@D@EC>B?@??>:ABAA=>A@=BACB?B@?7@C@?@@?@CA?=@?B@9>B@?D@9A%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJNNNNPNOKLKJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJKMMMNJMNNIMMMOLKMMIML	MD:Z:93T6	RG:Z:0.1	BI:Z:NNPQQQRQQOPOOPQPQNGGGNOLMMGPOPOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONORRRORRRNPQQPJINPMQP	NM:i:1	AS:i:95	XS:i:20
HWI-ST115_0148:1:4:8937:164749#0	1024	chr3	58125506	60	100M	*	0	0	CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA	4734;<>=?@>@BB@DBCCCBAD@DBBB?@>>>ABBB?A>B??EBCB?B?@9@CCBC@@CC@AC@ACCC@A@@E@=@BCBABB@A?A@@?A@>?A@<>=9	BD:Z:JJNNNNPNOKLKJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJKMMMNJMNNIMMMOOJOMIML	MD:Z:100	RG:Z:0.1	BI:Z:NNPQQQRQQOPOOPQPQNGGGNOLMMGPOPOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONORRRORRRNPQQPPLOPMQP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:62:7763:195878#0	1024	chr3	58125506	60	100M	*	0	0	CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA	4724;<>=?@?>AA?DBBABBAD@DBBB>@>>=@BBB?@?B??DBBB?B??7@BBAC?@BB?AB?@CBB?AA?C@?@ABA@DB?@>@@?>A@>@@@:=;5	BD:Z:IIMMNMQMNLLKJKLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKJAJJLIJAKIJKIJAAKMLOMNMLMJJLLMNILMOJNLNONKNMIML	MD:Z:100	RG:Z:0	BI:Z:MMPPPQSPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOQOPQOOPQQQNQQRNPRQPOMNPLPO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:23:14784:196081#0	16	chr3	58125506	60	100M	*	0	0	CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTCTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA	:@:?B@?C@C@BBAABCCCBA@A@C@B?C???@BCBE@?BD9@BBA?BE?7@CCA?E@A@E@CD@CCCE@C?AC?@A?A<@AD?=A@C>?@>>B<<6533	BD:Z:OLKJMNNLLLKKMNNLBBBKNHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHLJNHJIHJAAIKJMMMKLLJKKLMLHLKKHLMKLNJNQMNLJJ	MD:Z:59T40	RG:Z:0.1	BI:Z:QPONNOPPOPPPQRRQIIIOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMPOQMONMOHHNPNNPPONPNOONPPLPPPMQONPOMQRPQPNN	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:26:20630:27949#0	1040	chr3	58125506	60	100M	*	0	0	CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA	7?8=@?>BACABC@?BBBB7@@@@BBA?B>>=;@9BB@?BD?@BBB>BD?7@BCA>D@BBD@BA@BBBD@C??B@@@@A>?BD@>C@D@AC??B=93624	BD:Z:NKJIMNNLLKKKMMNKAAAJMIIKAJLLLJJLMKAIILLMMNJAJLLLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKHLLJMMINPMNLII	MD:Z:100	RG:Z:0	BI:Z:PPONNOPOPPPOQQQQHHHOQNNPGOPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLRROPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:2:18389:84462#0	0	chr3	58125507	60	100M	*	0	0	TGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATAT	43469<=<@A>BB@CBCCCCAD@EBBB?@>>>ABBB?@>B??DBBB>B??8@CCAC@@CC@AC@@CCC@AA@E@?ABEBAEC@@?A@A>B??=BA>@<:;	BD:Z:JJNKNQOOLLKKKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJMMMNJMNNIMLMONKONIMLL	MD:Z:100	RG:Z:0.1	BI:Z:NNQORRRQOPOOPQPQOGGGNOLLNGOPPNJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQRRORRRNPQQPQMPNMQPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:67:20358:45569#0	16	chr3	58125507	60	100M	*	0	0	TGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATAT	?;>???C?E?CBAAB@CCCAAAACDB=B????BBCD@?BD@@ACB?BE?6@BB@@D@BBD@CD@BBBDAC??C@@A@A>?@D@>B@D@AC@?C?<86264	BD:Z:LKJLNMMLLLKMNNLBBBKNIIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLKLMJMPMQLLJJ	MD:Z:100	RG:Z:0.1	BI:Z:PONPNOOOPPPQRRQIIIPQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOPOPNOOOONPPLOPPMQONPOMPROSPPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:24:19376:128752#0	0	chr3	58125508	60	100M	*	0	0	GAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATG	45649;<?>@B@?CABCCBAD@EBCB?@>>>>>CCA@?BA@DCAB>B??7@ACBC@ACC?AB?ABCC?A@?D@>@BE@>EC@A<BAA<A%%%%%%%%%%%	BD:Z:JJKKQPPLMKKLMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLMMNJMNNIMLLONJPNJMLLN	MD:Z:100	RG:Z:0.1	BI:Z:NNOPSRROPOOPQPQOHGGNOLLMHOOQNIJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQRORRRNPQQPQNQOKQPPQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:26:19751:62521#0	0	chr3	58125509	60	100M	*	0	0	AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA	46447>?@<CB?EBCBCBAD@DBBB>@>>>@BCC?A?B@@DBCB>B??8ACC@C?ACC@AC?ACCCAA@?D?>A@DBAFBAA@?AA>A?>9B@??>;;;;	BD:Z:JJKNPQMMLKLNKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLMNJMNNIMLLNNJOOJNLLNN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSRPPOOPQPQOHHGNOLLMGPOPOIIPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQORRRNPQQPQNRPLOPPQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:44:13221:170521#0	1024	chr3	58125509	60	100M	*	0	0	AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA	36349:=<6@A?CBCBBC@D@DCCB??>>?;BCC@@?BA@ECCA?B??8>CC@CAACC?ADAACCC???@D?>A@EBBFB@A@AAA>@?>>@?>C@;<<<	BD:Z:JJKNPQMMLKLNKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLMNJMNNIMLLNNJOOJNLLNN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSRPPOOPQPQOHHGNOLLMGPOPOIIPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQORRRNPQQPQNRPLOPPQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:65:10341:97177#0	1024	chr3	58125509	60	100M	*	0	0	AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA	36459;>=:BBACBCBBCAE@DBBB>@>>>ABCCA@?B?@DCBB?B??7@CBBD@@CCA@C@@CCC@A@@EA>@BCB?DB@A@A@@?A??4B;@B?9<:<	BD:Z:JJKNPQMMLKLNKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLMNJMNNIMLLNNJOOJNLLNN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSRPPOOPQPQOHHGNOLLMGPOPOIIPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQORRRNPQQPQNRPLOPPQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:45:5212:195541#0	16	chr3	58125509	60	100M	*	0	0	AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA	=>;9A@CABB@?CBBBB@?=?BBB?B>A>@CBBE@?BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@A@@>?BD@>C@D@AC@@D@?=>96854	BD:Z:ILLLKLLLLMNOLAAAJMIIKAJLLLJJLMKAIILLMMNKAJKLMMMJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKJLLHLNJQOPMKII	MD:Z:100	RG:Z:0	BI:Z:NPOPLNNPPRQQQHHHOQNNPHOQPQJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMRRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:65:19328:2272#0	0	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	44429=<<BA?DACCBBAE@EBC?>A>>>ABCC?@>B?@CCAB?B??8@BCAD@ACC@AC@@CCC@A?@E@?@BCBBFC?A@B@@>A?>8AB@C%%%%%%	BD:Z:JJNMQNNLLLNLLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNIMLLNMJONKNMLNNM	MD:Z:100	RG:Z:0.1	BI:Z:NNQQSPQOOPQPQOHHHNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNPQQPQNRQMPNPQQP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:66:3484:54472#0	1024	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTCTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	44439=<:AA?CAC@CBAD@DBBB>@>>>@BBB?@>B?@E?CB?B??7ACCAA@@CC@AC@ACCC@A??E@>A@C??DB@A?@@@>AA>A?B@EB;>:<<	BD:Z:JJNMQNNLLLNLLJJJIJKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNIMLLNMJONKNMLNNM	MD:Z:14T85	RG:Z:0.1	BI:Z:NNQQSPQOOPQPQOOONNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNPQQPQNRQMPNPQQP	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:7:12445:175053#0	1024	chr3	58125510	60	100M	*	0	0	TGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	43429>?@BB?EBCCBBAD@DBBB?@>>>ABBB?@>B??EBCB>B??7@CCAD?ACC@@C@@CCC@A?@E??@@CBAFC@A?B@@>A@>?@A@DB=>:<=	BD:Z:JJJMQNNLLLNLLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNIMLLNMJONKNMLNNM	MD:Z:0A99	RG:Z:0.1	BI:Z:NNNQSPQOOPQPQOHHHNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNPQQPQNRQMPNPQQP	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:7:1540:124569#0	1024	chr3	58125510	60	100M	*	0	0	TGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGCAGGGGACATATGAT	3456998:3<;B@@@BB?D@>??<<?</:8>>@<=?AA>ACCB>?=;4=AA7@AAB9????A=CC>@%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJJMQNNLLLNLLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNINNONJKMNKNMLNNM	MD:Z:0A84G3T10	RG:Z:0.1	BI:Z:NNNQSPQOOPQPQOHHHNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNRQRPKKQQMPNPQQP	NM:i:3	AS:i:89	XS:i:0
HWI-ST142_0240:1:2:19963:130770#0	1024	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	44439=<9@@?B@AABB@D@DBBB?@>>>@BBB@??B??DBBB>B@?8@BB@B?@BB?@B@?BAB@A??D??@@B@@DB?;????>@@>8@@>B@<<::8	BD:Z:IINLQNOLKLMKMJ@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IIMIJAJIJKIJAAKMLOMNMLMJJLLMNILMNIMKMNMJOOKOMLNML	MD:Z:100	RG:Z:0	BI:Z:MMQPRQQONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPONPQQQNQQRNPQPPPORQLPMPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:14161:187574#0	1024	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	44429==>@A?DBBBBBAD@DBBB?@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@CBB@A@?DA>@@BB@DB?@>@@?>A@><?A@C@<<8;8	BD:Z:IINLQNOLKLMKMJ@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IIMIJAJIJKIJAAKMLOMNMLMJJLLMNILMNIMKMNMJOOKOMLNML	MD:Z:100	RG:Z:0	BI:Z:MMQPRQQONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPONPQQQNQQRNPQPPPORQLPMPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:41:13983:94410#0	16	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	?;9?@CACCA@CCCCCA@A@CCC?DAAA@CCCEA?CE@@CCC?CE@8ACCA@D@BBD@CD@BBBEAC??C@@A@@>?BD@>C@D@AC@@EA?>@:;8564	BD:Z:LMLKLLMLNONLBBBKNIJKAJKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLILNKONOPLNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PPPOLNOPQRRQIIIPRNOOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPNOOOOOPPLOOOLQONPOMPQNQQQSPQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:47:12483:53170#0	1040	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	?;9??CABCAABCCCCA@A@CCC?CAAABCCCEA?BE?@CCB?CE@8ACCA@D@BCD@CE@BBCDAC??C@@@@@>?BE@>C@D@AC@@DA?>@:;8564	BD:Z:LMLKLLMLNONLBBBKNIJKAJKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLILNKONOPLNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PPPOLNOPQRRQIIIPRNOOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPNOOOOOPPLOOOLQONPOMPQNQQQSPQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:4:16748:194148#0	1040	chr3	58125510	60	100M	*	0	0	AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	=;:?@C@CCA?CCCCBA@A@@DC@EA?>ACCCEA?CE@@CCB?CE@8ABC@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA?>@:;8564	BD:Z:LMLKLLMLNONLBBBKNIJKAJKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLILNKONOPLNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PPPOLNOPQRRQIIIPRNOOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPNOOOOOPPLOOOLQONPOMPQNQQQSPQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:43:2720:132802#0	1040	chr3	58125510	60	100M	*	0	0	ACTAGAGAATCATTTTTCATAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT	5/7==AABB@;?AAAB@??(ABB?C@A>BCCBDA>BD@?BBB?BD?7@BB@@D@BBD@AC@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA?>@:;8564	BD:Z:NMLJKLLLNNOLBAAJMNLJAJLLLJJLMKAIILLMMNKAJLKMMMKJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKILLHKNJOOOQKMII	MD:Z:1G17C80	RG:Z:0	BI:Z:QPPNLNOORRQQHHHOQQQQHOQQPKJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPRQRPPMM	NM:i:2	AS:i:93	XS:i:19
HWI-ST115_0148:1:4:18448:107363#0	0	chr3	58125511	60	100M	*	0	0	GTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATG	44249;?AA?EBCCCB?D@EBBB>@>>>ABBB@@>B??EBBB>B??7@BBBC@ACC@AC@@CCC@AA@E@>A@C@AEC@A?A@@?A@>9@B@CB>A=<=;	BD:Z:JJMNNOMLMNLMJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMJMNNIMLLNMIONJOMMNNMN	MD:Z:100	RG:Z:0.1	BI:Z:NNQQQQPOPQPQOHHHOOLLMGOOQNIJOOOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQRRNPQQPQNRQNQONQQPQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:3:19432:186788#0	1024	chr3	58125511	60	100M	*	0	0	GTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATATGATG	4324=:.=7:@>><?BAD@DBAA>@>>>ABBB?@?A@?DAB@?B??7?BB?AA?@>@@:>@:@B=@9=B??A@C>>CA@@=@@?>@?<#;>>B9>>6148	BD:Z:IILMOONKLNKMJA@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILIJAKHJKIJAAKMLOMNMLMJJLLMNILMNIMKLNKKLOKONMNMLN	MD:Z:88T11	RG:Z:0	BI:Z:MMPPQQQNPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNOOQQQNQQRNPQPPKKRRMPONPPPP	NM:i:1	AS:i:95	XS:i:20
HWI-ST142_0240:1:65:19856:17943#0	1024	chr3	58125511	60	100M	*	0	0	GTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTTAGCAGGTCTCCATGTAGTGGAGGGGACATATGATG	43249<4A>>B@AAAB?D?DAAA>@>=>A;AAA>????DACA;A@?7?@@@>@ABB?@B?@BBAB?@>D@>@@B@>DA@@9?A?6>;=&6>>0?<>;;88	BD:Z:IILMOONKLNKMJA@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILIJAKHJKIJAAAJMOMNMLMJJLLMNILMNIMKLNKKLOKONMNMLN	MD:Z:64G23T11	RG:Z:0	BI:Z:MMPPQQQNPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGGPPQOPOOPNOOQQQNQQRNPQPPKKRRMPONPPPP	NM:i:2	AS:i:90	XS:i:20
HWI-ST115_0148:1:24:11998:133823#0	0	chr3	58125512	60	100M	*	0	0	TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA	3336:>AA@DBCCCCAD@DCBB<@>>>ABBB?@>B??DCCB<BA?7@BCAB@ACC@AC?ACBCAA??E@?A@B@?EC@A?@AA>A??9@B?DB>A?>;=;	BD:Z:JJNKONMMOLMK@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMIMNNIMLLNMINNJNNMONMNN	MD:Z:100	RG:Z:0.1	BI:Z:NNQORPPPQPQOHHHOPLLMGOOPOIIPONHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQRNPQQPQNRQNRPOOQPQQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:27:1639:51624#0	1024	chr3	58125512	60	100M	*	0	0	TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA	32269=?@>B=B??BAD@DBAB>@>;;?ABB@=?B@>CBBB>B??7@BBAC?@BB?@B?@BBC@A@?D@>@@B@>DB?@?@@?>A@>;@,8453=>=988	BD:Z:IIMKPNMLNLMJAA@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHJAKIIKIJAAKMLOMNMLMJJLLMNILMNIMKLMMJNNKONNOMLNM	MD:Z:100	RG:Z:0	BI:Z:MMPOQQPPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNPPQQNQQRNPQPPPNQRNQOPNPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:63:2606:53050#0	1024	chr3	58125512	60	100M	*	0	0	TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA	203;;5==<@AAAAAAD@DBBB>@>>>ABBB?@>B??DBAA>B??7@BBAB@?BB@?A@@BBC?A@>C?>@@B?>EB?@?@@A=A@?5>7:@=;===::7	BD:Z:IIMKPNMLNLMJAA@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHJAKIIKIJAAKMLOMNMLMJJLLMNILMNIMKLMMJNNKONNOMLNM	MD:Z:100	RG:Z:0	BI:Z:MMPOQQPPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNPPQQNQQRNPQPPPNQRNQOPNPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:65:11877:144770#0	1024	chr3	58125512	60	100M	*	0	0	TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA	213::<>@>BABBBBAD@=B@B=?>>>A?BB>??B@?>@BB:B@?7@BB@@@?BB?@B@@BBBA@>>C@?ABB@@BA?@?=?A>A???@?8>:8:>:78:	BD:Z:IIMKPNMLNLMJAA@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHJAKIIKIJAAKMLOMNMLMJJLLMNILMNIMKLMMJNNKONNOMLNM	MD:Z:100	RG:Z:0	BI:Z:MMPOQQPPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNPPQQNQQRNPQPPPNQRNQOPNPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:24:2929:82313#0	0	chr3	58125513	60	100M	*	0	0	AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	3445<>@@CBBBBBAD@DBBB>@>>>ABBB?@>B??EBBB>B??7ABBAC@@CC@@C@@CCC@A@@E@>A@CBADC@@?AA@?BA>A@B?DB?B@?>;<8	BD:Z:JJKLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:43:9326:76966#0	1024	chr3	58125513	60	100M	*	0	0	AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	2277;==8?@CCCC@CAECBB<A>>>>BCC?@>B??E@AB?BAA8@CCACAACC?AC@ACCC?A@@E@=A@B@?DB@A??@@?B==9:@-?1:?=?><%%	BD:Z:JJKLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:48:1537:53772#0	1024	chr3	58125513	60	100M	*	0	0	CGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	4*46:=>>@@BCCCAC?DCCA?@?>>ABCC?@?BA@ECCB>BAA7@CC@C?ACC@AC?ACCC@A?=D@>@@C@?E@@A??@@>AB,54,4A??B@?>:;;	BD:Z:JJNLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM	MD:Z:0A99	RG:Z:0.1	BI:Z:NNQPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:65:2864:31597#0	1024	chr3	58125513	60	100M	*	0	0	TGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCCCCATGTAGTGGAGGTGACATATGATGAC	3345;??>CABBBBAD@DBBB?A?>>ABCC?@?B?@DCCB?B??7@CCAC?@CCA@C@ACCC@B?>C??A@?>?BB@A??AA>A?>@@@??A;A%%%%%%	BD:Z:JJNLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMLIILLMILNNIMLLNMINMJNMNOOMNNM	MD:Z:0A70T28	RG:Z:0.1	BI:Z:NNQPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQOKKQQQNQQQNPQQPQNRQNRQPPOPQQP	NM:i:2	AS:i:94	XS:i:21
HWI-ST115_0148:1:8:6415:195009#0	1024	chr3	58125513	60	100M	*	0	0	AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	3356;??>DABBBBAD@DBCB?@>>>ABBB?@>B??EBCB?B??7ACCAC?@CC@AC@ACCC@AA?D?>A?C@?DBAA??AA>B@9@@A@EB?BA@>:<;	BD:Z:JJKLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:21:15989:132253#0	1024	chr3	58125513	60	100M	*	0	0	TGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	3456:>>>A@BBBBAD@DBBB?@>>>ABBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB@A@?E@>@@C@ADC?@>@=?>@@>@@<:6365=?;:79	BD:Z:IIMLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMLJNNJONNPNLNMM	MD:Z:0A99	RG:Z:0	BI:Z:MMPOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPPNQQNRPPPNPPPP	NM:i:1	AS:i:99	XS:i:21
HWI-ST142_0240:1:26:3977:39672#0	1024	chr3	58125513	60	100M	*	0	0	AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	3245:?>>B@BBBBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB@@@?E@>A@DBADC?@?@=?>@@>@?@@DB?B?>=:;6	BD:Z:IIKLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMLJNNJONNPNLNMM	MD:Z:100	RG:Z:0	BI:Z:MMOOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPPNQQNRPPPNPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:27:20026:127868#0	1024	chr3	58125513	60	100M	*	0	0	TGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATATGATGAC	3345=>?>BABBBBAD>DBBB>@>>>@BAB???B??CCBB>B??7@BBAC?@BB?@B?@@BB@A??D@>@BDB>BC?@?@;?>@?>&;91<*9==?;893	BD:Z:IIMLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMJKLNJONNPNLNMM	MD:Z:0A85T13	RG:Z:0	BI:Z:MMPOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPKKQQNRPPPNPPPP	NM:i:2	AS:i:94	XS:i:21
HWI-ST142_0240:1:62:8654:148039#0	1024	chr3	58125513	60	100M	*	0	0	AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC	4356:>@?BABBBBAD@DBBB?@>>>@BBB?@?B??DBBB>B??7@BBAC?@BB?@B?@BBB@A@?E@>ABBBADC?@>@@?>@@>@@?@CB?B??<::6	BD:Z:IIKLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMLJNNJONNPNLNMM	MD:Z:100	RG:Z:0	BI:Z:MMOOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPPNQQNRPPPNPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:64:12047:152217#0	16	chr3	58125514	60	100M	*	0	0	GAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACG	;><?AAABCCACA@A@CCB?DAAABCCCEA?CE?@CCB=CE?7ACCAADABCD@CD@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA@?B>AC?>=53+4	BD:Z:KKKKNOPMCCBKNIJKAKLLLIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMINLLNMPPONMJJ	MD:Z:100	RG:Z:0.1	BI:Z:NOOONPQQIIIPRNOPIPQPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPONQPLPOOLPNMOOMPQNQPPQPRRRQQNN	NM:i:0	AS:i:100	XS:i:20
HWI-ST115_0148:1:65:11545:192642#0	1040	chr3	58125514	60	100M	*	0	0	GAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTCGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACG	=?;?@A?BCCCCA@A@CCC?CAAABB@CEA?CE@@CCC?BE@7ACCAADABBD@@7@BBBDAC?AC@@@@@>?BD@>C@D@AC@@DA@?B>AC?>=54+4	BD:Z:KKKKNOPMCCBKNIJKAKLLLIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHLMKJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMINLLNMPPONMJJ	MD:Z:55T44	RG:Z:0.1	BI:Z:NOOONPQQIIIPRNOPIPQPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMPQOOHHNPNOQPPOPOPONQPLPOOLPNMOOMPQNQPPQPRRRQQNN	NM:i:1	AS:i:95	XS:i:20
HWI-ST142_0240:1:47:16052:133779#0	1024	chr3	58125515	60	95M5S	*	0	0	AGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATAGATC	3287;<BAAABBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?AA?D@?@@DBACC?A>@@?;A@>A?AADB>B?AA=;;;9	BD:Z:IIKJOPNNKBAAJKGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JIJKIIAAKMLOMNMLMJJLLMNILMNIMKLMLIMNJNMNPONMMKLM	MD:Z:95	RG:Z:0	BI:Z:MMONRRRPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPQMQQRNPQPPPNQQMQQRQPPMPOPP	NM:i:0	AS:i:95	XS:i:19
HWI-ST142_0240:1:5:17706:32015#0	0	chr3	58125515	60	100M	*	0	0	AGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGT	4756;=BAABBBAD@DBBB?@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@A?D@?@ACBAEC?A>@@?<@@>@@@@DB>B?@A=;;27	BD:Z:IIKJOPNNKBAAJKGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JIJKIIAAKMLOMNMLMJJLLMNILMNIMKLMLIMNJNMNPONOMMKL	MD:Z:100	RG:Z:0	BI:Z:MMONRRRPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPQMQQRNPQPPPNQQMQQRQPPNPPNO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:62:21194:135565#0	1024	chr3	58125515	60	100M	*	0	0	AGANTCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGT	23.%;;=>>>BBAD@DBBB>@>>>@BBB?@>B??DBBB?B??7@BBAC?@BB?@B?@BBB?AA?D@>@@BBAEC?A>@@?4@@><?>=>>>BAB?==3.7	BD:Z:IIKIMLNNKBAAJKGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JIJKIIAAKMLOMNMLMJJLLMNILMNIMKLMLIMNJNMNPONOMMKL	MD:Z:3A96	RG:Z:0	BI:Z:MMOMOORPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPQMQQRNPQPPPNQQMQQRQPPNPPNO	NM:i:1	AS:i:98	XS:i:19
HWI-ST142_0240:1:44:17812:198584#0	16	chr3	58125515	60	100M	*	0	0	AGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGA	<9==?>BBBBAA@@?BBB>C@@@ACBBEA?CD?@BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>A@D@AC@@DA@?B>BC@@@:4+54	BD:Z:JJJLNPMCCBKNJIKAJLLLJJLMKAIILLMMNKAJLLMMMKJAJKNHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLINLLNLNPNQLKII	MD:Z:99T0	RG:Z:0	BI:Z:NONPNPPHIIOQNNPHOQQQKKPQPHNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPRRRONMM	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:46:21053:166072#0	1040	chr3	58125515	60	100M	*	0	0	AGAATCATTTTTCACAAATAGGGGATTTGTATACAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGT	%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%>=@=>=A<8>87+02	BD:Z:JJJLNPMCCBKNJIKAJLLLJJLMKAIILLLLIKAJLLMMMKJAJKNHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLINLLNLNPNQLKII	MD:Z:32G67	RG:Z:0	BI:Z:NONPNPPHIIOQNNPHOQQQKKPQPHNLOOPPMOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPRRRONMM	NM:i:1	AS:i:95	XS:i:20
HWI-ST115_0148:1:67:16722:167166#0	0	chr3	58125516	60	100M	*	0	0	GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGCGACATATGATGACATG	3467;B@BBBC?C@EBBA?@>?>/ACC@>>BA?CA>A>A@?7ACC@C@AC@A@<?ACCC@A??D?=@;C??<B@B@B@@=A?%%%%%%%%%%%%%%%%%%	BD:Z:JJKLQOOLBAAKKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHMLLNNLNMIMLMOOOOOMIMN	MD:Z:83T13G2	RG:Z:0.1	BI:Z:NNOPSQROHHHOPMMNHOOPNIIOPNGOMPPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPQPPORQNRQQRRPPOPMQQ	NM:i:2	AS:i:92	XS:i:19
HWI-ST142_0240:1:43:18744:44408#0	1024	chr3	58125516	60	100M	*	0	0	GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGAG	3568;@@AAABAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?A@@D@>@ABBAEC@@?@@?>4?>>@@>B?<BAAA?%%%%%	BD:Z:IIJKQNPKBBAJKHHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHJKIJ@AKMLOMNMLMJJLLMNILMNIMKLMLIMMJNMMPONPNMKML	MD:Z:98T1	RG:Z:0	BI:Z:MMNPRRRNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPNPQRNPQPPPNQQMQPRRQPPNPNPO	NM:i:1	AS:i:98	XS:i:19
HWI-ST115_0148:1:6:1840:111707#0	16	chr3	58125516	60	100M	*	0	0	GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG	;>==>BC@CCA@A@CC=@EB?BBBDCD@?BD?AB?B?BE;7@BCBAEACCE@CBACCCEAC@ACAAAAA>?BEA?C@E>@D?@EA@@B?BE@AC>:,493	BD:Z:KKMNOMDCCLNIJKAKLLLJJLMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKLMLONNQPLIJJ	MD:Z:100	RG:Z:0.1	BI:Z:OOPQOOHIIPRNOPIPQQQKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOPPMOOPLPNMONLPQNQPPQPQQQRSOMNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:46:18554:196838#0	1040	chr3	58125516	60	100M	*	0	0	GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG	6<=;=@BB@B@@@@BBB?B@?=@ABAC??BE??CBB>CD>7>CB@@D@BBD@CB@BBBDAC?AB@@@@A>?BD@>B@D@AC=@D:@?B>AD@AB=:+484	BD:Z:JJLMOMCCCKNJJKAJLLLJJLMKAIILLMMNKAJLLMMMKK@JKNIJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHNLLMLNNNPPKHII	MD:Z:100	RG:Z:0	BI:Z:ONPPNPGHIPQNNPHOQQQKKPQQGOLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPRRQNLMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:5:7801:61872#0	1040	chr3	58125516	60	100M	*	0	0	GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG	<==;=ABCAB@@A?BBB?C@@@@BBCCA?CE??CBB>CD?7@BB@@C@BBD@BC@BBBD@C?@C@@@@@>?BD@>C@D@AC@@D?@?B>BD@AB=9+484	BD:Z:JJLMOMCCCKNJJKAJLLLJJLMKAIILLMMNKAJLLMMMKK@JKNIJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHNLLMLNNNPPKHII	MD:Z:100	RG:Z:0	BI:Z:ONPPNPGHIPQNNPHOQQQKKPQQGOLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPRRQNLMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:7:2991:103346#0	1040	chr3	58125516	60	100M	*	0	0	GAATCATTTTTCACAAATAGGGGATTTGTATGCAAACATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG	399==??AAA=>>?::@8A4>>>BBBC@?BD?@BB@@?C>3>BBA@B?BBD@AD@AAB=>A<788=@<>2>>C?>@=B==B=?C??=?>@@===/;/372	BD:Z:JJLMOMCCCKNJJKAJLLLJJLMKAIILLMMNKAJINMMMKK@JKNIJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHNLLMLNNNPPKHII	MD:Z:36T63	RG:Z:0	BI:Z:ONPPNPGHIPQNNPHOQQQKKPQQGOLOOPOPOGMMPPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPRRQNLMM	NM:i:1	AS:i:95	XS:i:20
HWI-ST115_0148:1:5:3749:99449#0	0	chr3	58125517	60	100M	*	0	0	AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC	3656?>@ABB?C?DBBB?@>><BACC@?<;@?BC5B:A@>7@C;@CA@CC@@??>7?=?0==A>7A?A=<=>?;=?%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJLNOPMBBAKLHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLLLNMINMIMLLOONPONLMIN	MD:Z:100	RG:Z:0.1	BI:Z:NNPQRRPHHHOPMMNHPOPNIIOOOGNNPOQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPPQNRQNRQQRRQQPNOQMQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:65:19877:44416#0	1024	chr3	58125517	60	100M	*	0	0	AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAAGTGC	3656?>@AAA?C?DCBA=?>>>=BCC@??B@?CCCB?B@?7ACC@B@ACC@ABAA?BBAA??D>=A@A@?DB@A??A@?>?<??<@%%%%%%%%%%%%%%	BD:Z:JJLNOPMBBAKLHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLLLNMINMIMLLOONPOLKNIN	MD:Z:95C4	RG:Z:0.1	BI:Z:NNPQRRPHHHOPMMNHPOPNIIOOOGNNPOQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPPQNRQNRQQRRQQPMPQMQ	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:47:6693:50311#0	1024	chr3	58125517	60	100M	*	0	0	AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC	3545>>;>>@:C><@=A:==<<:<8>>>:8@=A@<=<?@?7?@@>%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:IIKMOPMBBBJKHIH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIKIJA@KMLOMNMLMJJLLMNILMNIMKLMLIMMINMMOONPONKLIN	MD:Z:100	RG:Z:0	BI:Z:MMPPRRPGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMQPRNPQPPPNQQMQPQRRQPPNNOMP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:24:11281:81065#0	16	chr3	58125517	60	100M	*	0	0	AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC	>=;=ABBCCA@AACCB?DA?ABCCCE@?BE?@CCC?BE@7ACCAAEACBE@BD@BBBEAC?AC@@@@@>?BD@>C@D@AC@@DA@?B>BDAAC?<04834	BD:Z:KMNNMCDCLOIJKAKLLLJJMMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKMLNNMPPOINJJ	MD:Z:100	RG:Z:0.1	BI:Z:OPQQNGHIPRNOPIPQQQLKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQPLPOOMPNMONLOQNQPPQPQQPRRQMPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:28:14521:122053#0	1040	chr3	58125517	60	100M	*	0	0	AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC	;=;<@BCCCAAA@BCC?CB??BCDDE@?BD??CCB?BE99@CCABEA?DE@CCACCCEAC@@BAAAAA>?BEA<CAEAAAAACA@?B?BEAAD?<25733	BD:Z:KMNNMCDCLOIJKAKLLLJJMMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKMLNNMPPOINJJ	MD:Z:100	RG:Z:0.1	BI:Z:OPQQNGHIPRNOPIPQQQLKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQPLPOOMPNMONLOQNQPPQPQQPRRQMPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:8:10443:148224#0	0	chr3	58125518	60	100M	*	0	0	ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC	3539<>?@@?<=A=B?=?><=@AAA?><A@=??A>>?A?8/>@>AAA@@?@B@@BBB@>@>@?:?@B@>CB@@????>A?,6?9<DA?A%%%%%%%%%%%	BD:Z:IIMKQMDBBKKHII@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJIJAAJMLOMNMLMJJLLMNILMNIMKLMLIMMIMMMONNPOOLLINM	MD:Z:100	RG:Z:0	BI:Z:MMPPRPIGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPQQNPQPPPNQQMQPQQRRQPPLOMPO	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:47:6712:171630#0	16	chr3	58125518	60	100M	*	0	0	ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC	<=;>A@/A??A@;BB?C<?=?BBBD??BD?@CBB??D?7@CCA@D@CCD@CEAACCDAD@AC;@B>A<ABEA?@AE=AD?AE@@?B?BE?BD@>3;:323	BD:Z:MNNLCCDLOJJKAKLLLJJMNKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLLNMMOOOLNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQQPFGHPRNOPIPQQQLLOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQLOPOLQNMONLOPNQPPQPQQPQRPOPONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:63:14295:160964#0	1040	chr3	58125518	60	100M	*	0	0	ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC	;<;>ACCCBA<?;==?C>?<@BCBD@?BE?@CAB?BE?7=CCAAFACCFACD@CACDAC?ABAAA@A>A@D@?C;E?@DAAE@??B?AEAAD@=5;9213	BD:Z:MNNLCCDLOJJKAKLLLJJMNKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLLNMMOOOLNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQQPFGHPRNOPIPQQQLLOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQLOPOLQNMONLOPNQPPQPQQPQRPOPONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:7:6785:81212#0	1040	chr3	58125518	60	100M	*	0	0	ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC	=<<>ACB?A?AACCB@CB?>ACCCD@?BD?@CCB?BE?7?CCA@EACCF@BD@CBBDAC?AC@@A@@;?BD@>CAD@AC@@D???B>BDABC@>39:324	BD:Z:MNNLCCDLOJJKAKLLLJJMNKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLLNMMOOOLNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQQPFGHPRNOPIPQQQLLOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQLOPOLQNMONLOPNQPPQPQQPQRPOPONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:41:14818:141318#0	0	chr3	58125519	60	100M	*	0	0	TCATTTTTCATAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT	3477=?@A:B@9A>@;?>>?1BCAAA>B@;3:5?.B@>5ACC@AAACCAAA@ACCC@A>?D@>A@C@?EBAA?@A@>A??<@==BB?B@@@@=;4?<18=	BD:Z:JJLMNDCBLLMLIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKNMINMIMLLNNNOOOMNINNL	MD:Z:10C89	RG:Z:0.1	BI:Z:NNPQQIIHOPQPNHPPQNIIOONGOMPPPPPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPNRQNRQQRRQRRPNOMQPP	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:61:16724:48061#0	1024	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGGCATATGATGACGTGCCT	4586;>@A@E@DBBB>@>>>ABBB?@>B??EBCB>B??7@BBAC?@BB?@C?ACCC@AA@E@?A@DBAECAA?A@@?AB>>@>?DB?B@A@@B@7>=9:;	BD:Z:JJLMNDCBLLIIIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKNMIMNNMLLNNNOOOMNINNL	MD:Z:82A17	RG:Z:0.1	BI:Z:NNPQQIIHOPMMNHPPQNIIOONGOMPPPPPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPNPPQRQQRRQRRPNOMQPP	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:23:18737:20226#0	1024	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT	4586;>@A@D@DBBB?@>>>@BBB?@?B??DBBB=B??8@BBAC?@BB?@B?@BBB??@?D@?@ACB@BC@@?@@@>A??3@@@DB?B@?A=@=5<<77;	BD:Z:IIKMNDDBKLHIIAJKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHJAAKLLOMNMLMJJLLMNILMNIMKLMLIMMIMLMONMPOOMMINML	MD:Z:100	RG:Z:0	BI:Z:MMPPPIIGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPRMPQPPPNQQMQPQQQRRQPNMMPOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:25:11308:176882#0	1024	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT	3377<>?@>=;CB;>9?>><=@AA?;99<>DBB@;?@=8@B?@A@@B@@@;A@BBBAA=>D@8A?A@>CAA@>?@A>A@?0A@9C?=??=8?@>4<7859	BD:Z:IIKMNDDBKLHIIAJKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHJAAKLLOMNMLMJJLLMNILMNIMKLMLIMMIMLMONMPOOMMINML	MD:Z:100	RG:Z:0	BI:Z:MMPPPIIGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPRMPQPPPNQQMQPQQQRRQPNMMPOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:62:8076:104111#0	1024	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT	4586;>@A?D@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@?@ACB@DC@A?A@@>A@>=?A@DB?B@@B?A>6=;789	BD:Z:IIKMNDDBKLHIIAJKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHJAAKLLOMNMLMJJLLMNILMNIMKLMLIMMIMLMONMPOOMMINML	MD:Z:100	RG:Z:0	BI:Z:MMPPPIIGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPRMPQPPPNQQMQPQQQRRQPNMMPOP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:3:17353:40399#0	16	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA	;;<?@CCA@A?CCB?A?BBBBBCD=?BD?@>BB;BD?7@@CA?D@9CEBCE?CCCEBD@?DAAA?A>?BEA?D@E?AD?AEAA@A?BEABC??4<?4253	BD:Z:NNLBCCMOJKKAKLLLJJMNLAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKNMLONNLQNMJJ	MD:Z:99T0	RG:Z:0.1	BI:Z:QQPHFGORNOPIPQQQLLPQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMOOPLPOMONLOPMQPPQPQQPQQPNROONN	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:4:12205:180848#0	1040	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT	<<=>BBBA@@@CCC?D?AABBCDE@?CE?ACCC?BE@7ACCAAEACCD@@D@BBBDAC@?B@@@@@>?BE@?@@E@@C@@D@@?B>BDABD@?5<=3244	BD:Z:NNLBCCMOJKKAKLLLJJMNLAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKNMLONNLQNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QQPHFGORNOPIPQQQLLPQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMOOPLPOMONLOPMQPPQPQQPQQPNROONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:64:10493:106639#0	1040	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA	;;<?9:?=;8=B?C?EA??@BDCF@?BD?@BCB?BE@7@CCA?EBCCE@CDACCCCBD@@D?@>@A>?AD@?DAE?@A?@E>??B?BE?BE@?6>?4133	BD:Z:NNLBCCMOJKKAKLLLJJMNLAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKNMLONNLQNMJJ	MD:Z:99T0	RG:Z:0.1	BI:Z:QQPHFGORNOPIPQQQLLPQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMOOPLPOMONLOPMQPPQPQQPQQPNROONN	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:25:4650:187088#0	1040	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTGTGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT	8:<=@ABA@@?BCB>C@@@ABBADADAD@@CBB?BD?8@BBA@D@BBD@BD@BBBDAC??C@@A@@>?AD@>C@D@AC@@DA@?B>BDABD@?5<=3244	BD:Z:MNKABCLOKJLBKLLLJJLMKAIIHIHMNKAJLLMMMKKAJKMIKAIHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKMKMMLNMNKQMMII	MD:Z:26A73	RG:Z:0	BI:Z:PPPGEGNQOOPHOQQQKKPQQHOMLMLOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPOPNQOOMM	NM:i:1	AS:i:95	XS:i:0
HWI-ST142_0240:1:44:10947:22007#0	1040	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA	8:==@ABA?@?BCB?C@@@ABBBDA?CE@@CBB?BD?8@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA??B>BDABD@?5<=3124	BD:Z:MNKABCLOKJLBKLLLJJLMKAIILLMMNKAJLLMMMKKAJKMIKAIHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKMKMMLNMNKQMMII	MD:Z:99T0	RG:Z:0	BI:Z:PPPGEGNQOOPHOQQQKKPQQHOMOPPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPOPNQOOMM	NM:i:1	AS:i:99	XS:i:19
HWI-ST142_0240:1:68:15360:190460#0	1040	chr3	58125519	60	100M	*	0	0	TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA	9:<=@AB@?@?BCB?C@@@ABBBDA>AC@@CBB?BD?8@BBA@D?CBD@CD@BBBDAB??C@@A@@>?BD@>C@D@AC@@D<@?B>BDABD@?5<=3234	BD:Z:MNKABCLOKJLBKLLLJJLMKAIILLMMNKAJLLMMMKKAJKMIKAIHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKMKMMLNMNKQMMII	MD:Z:99T0	RG:Z:0	BI:Z:PPPGEGNQOOPHOQQQKKPQQHOMOPPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPOPNQOOMM	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:23:6094:167071#0	0	chr3	58125520	60	100M	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA	3857=?A>CACBBB>>=>>=BCC@A?B??EACB>B??8@CCAC@@CC?@CA@CCCAA?=D?>@@D@AEB?A>?A@>A?>@@A@DB?BAABAAA5@>:9>%	BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOONNNJNNLL	MD:Z:100	RG:Z:0.1	BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQOPKQPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:26:13769:48947#0	1024	chr3	58125520	60	93M7S	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACAGATCGG	3866;?A>D@DBCB>?==>@BCC?@>B??ECCB?B??7@BB@C?@CC?AC?ACCC@AA?E@=A@DBABBAA??@@>A?:A@A@DB?B@ABA@@C??:;29	BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOONKPLMNLM	MD:Z:93	RG:Z:0.1	BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQMPMPQOO	NM:i:0	AS:i:93	XS:i:19
HWI-ST115_0148:1:46:3303:72160#0	1024	chr3	58125520	60	92M8S	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAGATCGGAA	3856;@@@C@DBBB>@>>>@BBC?A?B??EBBB>B??7@BA@C@ACC?@C?@CCC@AA@E@>ABDBADC@A?AAA?A??4@=@DB?B@AB@A??=>496;	BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOOMMNOLMLK	MD:Z:92	RG:Z:0.1	BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQOOOOOPO	NM:i:0	AS:i:92	XS:i:19
HWI-ST115_0148:1:4:18275:87094#0	1024	chr3	58125520	60	100M	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA	4866;>@@C@DBCB>A>>>ABBB?@>B??DBBB>B??7@BBAC?@BB?@B@@CCC@A@@EA>@BCBAEC@A?AA@>@@??A@@CB=BABA@B?5@?;9>8	BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOONNNJNNLL	MD:Z:100	RG:Z:0.1	BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQOPKQPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:43:2951:146335#0	1024	chr3	58125520	60	98M2S	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCAC	4756;>>?C?CBBB?>===?8AA?==A@>CBBB?B??8@BB@A?@BB??B?@BBB?B@?D@>@@C@?D>@@?@@@>A?>5)::>?>A@A@?A@7?%%%%%	BD:Z:IIMJEDDKLIIIAKKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHIAAKMKOMNMLMJJLLMNILMNIMKLMLIMMIMLLONMOOOMNJNMLH	MD:Z:98	RG:Z:0	BI:Z:MMPNIIIOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQNOQPPPNQQMQPQQQQRRQNOKPOPL	NM:i:0	AS:i:98	XS:i:19
HWI-ST142_0240:1:8:13137:17336#0	1024	chr3	58125520	60	92M8S	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAGATCGGAA	4756;>@?C?BBBB>@>>>?BBB?@?B??DBBB?B??7@BB@C??BB??B??BBB?B@>D?>?>B@@CA?A?@@@>A?>@8@=A@=??A5@B?A9<36:%	BD:Z:IIMJEDDKLIIIAKKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHIAAKMKOMNMLMJJLLMNILMNIMKLMLIMMIMLLONMOONMNNKLKJ	MD:Z:92	RG:Z:0	BI:Z:MMPNIIIOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQNOQPPPNQQMQPQQQQRRPOPNNMPN	NM:i:0	AS:i:92	XS:i:19
HWI-ST115_0148:1:22:12391:16829#0	16	chr3	58125520	60	100M	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA	;=<?@AAAAACCB?BB?BBCCCFB@BD@@B@B?BE@7@CDAAEACCEA=EACCCEAC@AB?@AAA>@BEA?DAE?@B?@DA@?B?BE?BDA?7?B:3423	BD:Z:NLBBCLPJKLAKLLLJJMNLBIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMMLNNMKQQNNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPHHFNQNOPIPQQQLLPRPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPOOMPNNONLOPMPPPQPQQPQQONQQOQNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:1:18497:115417#0	1040	chr3	58125520	60	100M	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA	9<;=@AA???BBC>C@@?ABBBD@?BE@@BCB?CD?7?BB@@D@BBD@BD@BBBDAC??C@@A@@>?BD@?C@D@AC@@DA@?B>BDABDA?6>@82424	BD:Z:NKAABLOKKLBKMLLJJLMKAIILLMMNKAJLLMMMKKAJKNHKAIIJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLKMMKNMLKPQMMII	MD:Z:100	RG:Z:0	BI:Z:PPGGENPNOQHOQQQKKPQQHOMPOQOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONNQQOPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:42:16985:26745#0	1040	chr3	58125520	60	100M	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA	8===@A@@@?BBC?C@@>ABBBD@?AD@@BCB?@D?7ABB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>B@D@@C@@C@@?B>BDABDA?6>@82424	BD:Z:NKAABLOKKLBKMLLJJLMKAIILLMMNKAJLLMMMKKAJKNHKAIIJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLKMMKNMLKPQMMII	MD:Z:100	RG:Z:0	BI:Z:PPGGENPNOQHOQQQKKPQQHOMPOQOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONNQQOPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:7:15321:9803#0	1040	chr3	58125520	60	100M	*	0	0	CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA	:=<=@A@?@?BBC?C@@@ABBBD@?BE?@CBB>CD?8ABB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@A@AC@@DA@?B>BDABDA?6>@82423	BD:Z:NKAABLOKKLBKMLLJJLMKAIILLMMNKAJLLMMMKKAJKNHKAIIJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLKMMKNMLKPQMMII	MD:Z:100	RG:Z:0	BI:Z:PPGGENPNOQHOQQQKKPQQHOMPOQOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONNQQOPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:22:19130:140774#0	0	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	4666;>?D?DBBB>@>>>@BBBA@>B??EBAB?B??8@BBAC?@CC?AC?ABCC@AA@EA>ABD@ADC@A?@AA?@?>>BA@DB?BAA?AA@7@@=;<:<	BD:Z:JJKADDMMJIJBKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLINMIMLLNNMNONMOJONLLL	MD:Z:100	RG:Z:0.1	BI:Z:NNOHJIPPMMNHPPQOJIOONGNMQOPQOMHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQQNRQQRRQRRQPQLOPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:8:14113:59358#0	1024	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	4666;>?C?DBBB>@>>>@BBB@@?B@?DBBB>B??7@BCAC?@BA?@A?ACCC@AA>E@=A@C@ADB@A?@AA=A>>?@B@DB?B@AB@A=7AA7:<8;	BD:Z:JJKADDMMJIJBKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLINMIMLLNNMNONMOJONLLL	MD:Z:100	RG:Z:0.1	BI:Z:NNOHJIPPMMNHPPQOJIOONGNMQOPQOMHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQQNRQQRRQRRQPQLOPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:8:9706:101181#0	1024	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	3568=??D@DCCA?A>==@ACC???A??EC>B>BA?7@CCAC?@CC?@C?ADCC@A=?D?>A@C@?CB@A??@A=@=<;@A@DB?B@A229,2=859<8;	BD:Z:JJKADDMMJIJBKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLINMIMLLNNMNONMOJONLLL	MD:Z:100	RG:Z:0.1	BI:Z:NNOHJIPPMMNHPPQOJIOONGNMQOPQOMHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQQNRQQRRQRRQPQLOPPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:13388:89156#0	1024	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	4666;>?C?DBBB>@>>>@BBB??>A??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@BCA@EB?A?A@@>@@>@?<@DB?B?AB?@@7@>=9<9=	BD:Z:IIJAEDMLIJIAKLLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@AKMLNMNMLMJJLLMNILMNIMKLMLIMMIMLLNNMONOMNKOMLLL	MD:Z:100	RG:Z:0	BI:Z:MMNGIIQPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMPPPPPNQQMQPQQQQQRROOMNOPPP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:27:5834:78263#0	16	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	=>>?AA@AAC@B?B????BCCE@?BD?ACBB?BD?7@CCAAE@BBEACE@CCBDAC??C@@A@?>ABE@?C@E@AC@@E@A?A?ADABDA@6?B;74264	BD:Z:LBBBLOKKLBKLLLJJMNLBJHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLLNMMJPQQNLJJ	MD:Z:100	RG:Z:0.1	BI:Z:PHHHMPMOPIPQQQLLPRQHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPOLQNMPNLOPMPOPQPQQPQQOMQPQQPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:41:2831:14874#0	1040	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	<>;?AAA@@CCC?C?A?@BACEA?CD?ACC@:BE?7ACCA@EABCEABEABBCDAC?AC?@A@A>?BE@?C@D@AC?@E>@?A>AEAADA@6?B;74263	BD:Z:LBBBLOKKLBKLLLJJMNLBJHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLLNMMJPQQNLJJ	MD:Z:100	RG:Z:0.1	BI:Z:PHHHMPMOPIPQQQLLPRQHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPOLQNMPNLOPMPOPQPQQPQQOMQPQQPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:25:19258:173665#0	1040	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGTCTAT	7;6=?@??@?BB?C@>?=B@BC@>BD?AAA@?AC>7@BBA@D@AAB<@D?BBBD@B>AB>?A??>??D@=B@A;@C;<B???A>AD?BC?<5=@;<4243	BD:Z:KAAAKOKKMBKMMLJJLMKAIILLMMNKAJLLMMMKKAJKNIJAIIKHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJMMKMMLILPOMLII	MD:Z:95C4	RG:Z:0	BI:Z:PGGGLPMNQIOQQQKKPQQHOMPPPPPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLNRQPOMM	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:5:16655:34005#0	1040	chr3	58125521	60	100M	*	0	0	ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT	;<:=@?@A?BB;?C@>>@A@BD@>BD??BB@??D>8@BB@?D@BBD@BD@BBBD@C>?C@@@@A>@BD??@@D@@A@@D??=B>BB@BC?>7?B;84254	BD:Z:KAAAKOKKMBKMMLJJLMKAIILLMMNKAJLLMMMKKAJKNIJAIIKHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJMMKMMLIPPQMLII	MD:Z:100	RG:Z:0	BI:Z:PGGGLPMNQIOQQQKKPQQHOMPPPPPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLQQQPOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:23:6146:104545#0	0	chr3	58125522	60	100M	*	0	0	TTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCGGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATC	3666==B@DBBB>@>>>ABCB?A?B?@ECCB?B??7@CCAC?@BB@@C?ACDC?AA>7?>A@D@?DBAA?@BA>B?>>@A>DB?B4<<:7=6@%%%%%%%	BD:Z:JJAADNNJJJBLKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNKLILMJJLLLMILMMHLKKMLHNMIMLLNNMNNNMNKOOLLLN	MD:Z:57A42	RG:Z:0.1	BI:Z:NNHHJPQMMNHPPQOJJOONGNMPPPPPMGPPPQQNQNHOOPNNHONNONNHHOQPRNOJPQONOQQQNQQQMOPPOPMQPNRQQRRQRRQPRMPNPPPQ	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:26:17568:111462#0	16	chr3	58125522	60	100M	*	0	0	TTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATC	=<==>>@?BBB?D@@@@BB?D@?AE?@CCC?BD@7@BC@@D@BBD=BD@BBBDAC??B@@@@@>?BD@>B@D@AB@@DA@?A?BDABDA@7?C=:92644	BD:Z:AAAJNKKMCKMMMJJLMKAIILLMMNKAJLLMMMKKAJKNIK@IIKIHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLMKMLLINPPQLLII	MD:Z:100	RG:Z:0	BI:Z:GGGNNMMPIPQQQKKPQQHOMPPQOQOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOQQROPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:48:19526:151624#0	1040	chr3	58125522	60	100M	*	0	0	TTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATC	;:<=??@@?B>?B>>??A<8C??BC>@3:=>>D?7?BBA@D?<BB@CB@BBCC@A<?>;-A>@>@>@??A;A<<8=>C=>?><AB?<A=:6>B<;:4543	BD:Z:AAAJNKKMCKMMMJJLMKAIILLMMNKAJLLMMMKKAJKNIK@IIKIHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLMKMLLINPPQLLII	MD:Z:100	RG:Z:0	BI:Z:GGGNNMMPIPQQQKKPQQHOMPPQOQOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOQQROPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:23:21094:189737#0	0	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCT	4668:@>CBBB>@>>>>ABB?A>A@?ECCB?B??7@BB@C@@CCAAC@@BCCAA@>B?>A@C?>CB@A?@@@>@@>:B@3BB=@BB@<A16??<2@::73	BD:Z:JJAANOKJKBLLMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMMIMLLNNMNNMMNJPOMLLNK	MD:Z:99C0	RG:Z:0.1	BI:Z:NNHHQQNMNHPPQOJJPONGNMPOQPONGOPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMRQQRRQRRQPRNQONPPQO	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:24:7477:78408#0	1024	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC	4666:B>CABB>@>>>@BBB?@?B??DBBB>B??7@BBAC?ABC?AB?@CCB@@A@EA?ABCBAEC@A?AA@>A?>AAA@EB?BABB@A?7A@??A;<;8	BD:Z:JJAANOKJKBLLMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMMIMLLNNMNNMMNJPOMLLNM	MD:Z:100	RG:Z:0.1	BI:Z:NNHHQQNMNHPPQOJJPONGNMPOQPONGOPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMRQQRRQRRQPRNQONPPQO	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:43:9326:167668#0	1024	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATTC	4666:@>CABB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACB@DB@@>A@@?A@=@?A@/?=??AB@A@7?@>=?97&7	BD:Z:IIAANNKJJBKLMMIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JMLOMMMLMJJLLMNILMNIMKLMLIMMIMLLNMLONNLNKPOMLLJJ	MD:Z:98C1	RG:Z:0	BI:Z:MMGGQRNLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPPOPNQQMQPQQQQQQQPQNPONPPNO	NM:i:1	AS:i:98	XS:i:19
HWI-ST115_0148:1:28:4544:44664#0	16	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC	;===>@ACCB?DAAABCCCE@@CFA?CBB?BE?8ACCA@DABCEABEADBCDAA??CAAB?A>@?E@>D@E?@B?@E@@?B>BDABDA?7@C><;76423	BD:Z:BBKNJKMBLMLLJJMNLBJIMKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMMLIOOPQOMLJJ	MD:Z:100	RG:Z:0.1	BI:Z:HHOQKMOIPQQQLLPRQIOMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMPNNONMOPMPOOPPQQPQQOMPOPRRPPNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:22:13125:90542#0	1040	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC	9;;<>?@BBA>?A@>AABB;@>BD@ABBA?@D?8@BBA@D@ABB@BD@BBBDAC??C@@A@@>@BD@>B@D>AC@@DA@?B>BDABDA@7@C>;<76424	BD:Z:AAJMJKMCLMMMKJLMKAIILLMMNKAJLLMMMKKAJKNIKAHIKIIJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLKMLKINNPPPLKII	MD:Z:100	RG:Z:0	BI:Z:GGNPKMOHPRQQKKPQQHOMPPQPPPGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOQRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:28:12239:165270#0	1040	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC	9<;;=?@BBB=B??=@B?CA@?BD??BCC?AD?7@BBA@D@BBD@BD@BBBCAC??B?@A@B>?BD@?C@D@AC@@D?@?B>BDABDA@7@C>;<88423	BD:Z:AAJMJKMCLMMMKJLMKAIILLMMNKAJLLMMMKKAJKNIKAHIKIIJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLKMLKINNPPPLKII	MD:Z:100	RG:Z:0	BI:Z:GGNPKMOHPRQQKKPQQHOMPPQPPPGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOQRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:61:10099:38446#0	1040	chr3	58125523	60	100M	*	0	0	TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC	;=;;=?@BBB>CA@>ABBBD@>BD@@BBA?AD>8@BBA@D@BBD@BD@BBBDAC?@C@@@@A>?BD@>C@D@AC@@D?@?B>BDABDA@7@C><<76424	BD:Z:AAJMJKMCLMMMKJLMKAIILLMMNKAJLLMMMKKAJKNIKAHIKIIJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLKMLKINNPPPLKII	MD:Z:100	RG:Z:0	BI:Z:GGNPKMOHPRQQKKPQQHOMPPQPPPGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOQRQPPMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:21:5903:122469#0	0	chr3	58125524	60	100M	*	0	0	TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	4665=<BAAB>@>>>@BBB?A?B@?DCCB>B??8@BCAC?@BB?@B?ABBC?AA@EA>A@CBAEC@A?AA@>A@>AAAADC?CAABAA@7AA?@B>>::<	BD:Z:JJAKOLKKCLLNMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLIMLLNNMNNMLNJOPMMLNMJ	MD:Z:100	RG:Z:0.1	BI:Z:NNHORNNNHPPQOJJPPNGNMPOPQOMHOOPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQQQRRQRRQPRNRPONPQOK	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:43:7687:177083#0	1024	chr3	58125524	60	100M	*	0	0	TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	4665=<BAAB>@>>>@BBB?@>B??DBBB>B??8@BBAC?@BB?@B?@B?C?AA@EA?ABDBAEC@@?AAA>A?>?@B@DB?BAACAAA7A@?@B>>:;:	BD:Z:JJAKOLKKCLLNMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLIMLLNNMNNMLNJOPMMLNMJ	MD:Z:100	RG:Z:0.1	BI:Z:NNHORNNNHPPQOJJPPNGNMPOPQOMHOOPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQQQRRQRRQPRNRPONPQOK	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:28:17653:40535#0	1024	chr3	58125524	60	100M	*	0	0	TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	4665=<BAAB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACAADB?A>@@@?AA>@?@@D??B?@?@A?7@?>@B=<:89	BD:Z:IIAJOKLJBLLMMJIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLLOMNLLMJJLLMNILMNIMKLMLIMMIMLLNMLNNNLMKPONMLMLJ	MD:Z:100	RG:Z:0	BI:Z:MMGORNNNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOPONQQMQPQQQQQQQOQOQOPNPPOJ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:6794:128447#0	1024	chr3	58125524	60	100M	*	0	0	TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	4665=<BAAB>@>>>@BBB?@?A??DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@>@ABAADB?A>@@@>A@>>?A@C??B@AB@A<7@?=@A<=<7;	BD:Z:IIAJOKLJBLLMMJIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLLOMNLLMJJLLMNILMNIMKLMLIMMIMLLNMLNNNLMKPONMLMLJ	MD:Z:100	RG:Z:0	BI:Z:MMGORNNNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOPONQQMQPQQQQQQQOQOQOPNPPOJ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:8:15977:151184#0	1024	chr3	58125524	60	100M	*	0	0	TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	4566=<B@AB>@>>>@BBB???A??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@@BAADB?@?@@@>BA>>?A@D@?B?AB@A?6@?>@B<=::7	BD:Z:IIAJOKLJBLLMMJIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLLOMNLLMJJLLMNILMNIMKLMLIMMIMLLNMLNNNLMKPONMLMLJ	MD:Z:100	RG:Z:0	BI:Z:MMGORNNNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOPONQQMQPQQQQQQQOQOQOPNPPOJ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:43:17533:112959#0	16	chr3	58125524	60	100M	*	0	0	TATCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	5*;;>?BBB>C@=@@BBBD@>BD?@BBB?CE>7ABB@AD@BBD@BD@BBBDAC??B@@@@A>?BD@>C@D@AC@@D?@?B>BDABB@?8?D><>:;4224	BD:Z:LLMIJMCLNMMKKLMKAIILLMMNKAJLLMMMKKAJKNIKAIHKIIK@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJMLKHNNNPOPKJII	MD:Z:1T98	RG:Z:0	BI:Z:OPPMKOGORRQKKPQQHOMPPQPQOHNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOORQRPJMM	NM:i:1	AS:i:98	XS:i:19
HWI-ST142_0240:1:67:8529:131204#0	1040	chr3	58125524	60	100M	*	0	0	TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC	=;;:=>BBB?B@>:@BBBC@?BD?@BBC?CE?7ABB@AD@CBD?BD@BBBDAC?@C@@@@A<?BD@?C@A@@B=@D7@?B>BDABDA@8@D>=>:;4224	BD:Z:AJMIJMCLNMMKKLMKAIILLMMNKAJLLMMMKKAJKNIKAIHKIIK@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJMLKHNNNPOPKJII	MD:Z:100	RG:Z:0	BI:Z:GNPMKOGORRQKKPQQHOMPPQPQOHNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOORQRPJMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:64:11727:57771#0	0	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	46599@AAA??==>ABBC?@>BA?DBBB?BA?7@CCAC?@BB??A@@C>CAAA@D??@@>@?DBAA<?>A=@>=4?>;C?9?10??@*37<*>5<@<9:>	BD:Z:JJKLLLLCMLNNIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHMLLNNMNNMLMJOONMMNMJM	MD:Z:100	RG:Z:0.1	BI:Z:NNOPONOHPPQOJJPPOGNMPOPPPMGPOOQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPQRRQRRQPRNRQPONQOKQ	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:26:10573:112719#0	1024	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	46589@@AA>@>>>@BBA?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ABA@EB?@?@A@>A@><?A@DB>B?AB@B@7???@?>>:77:	BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL	MD:Z:100	RG:Z:0	BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:41:15139:143134#0	1024	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	46589@@AA>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB@@@?D@>@ACA@EB?@?@A@>A?>??A@DB>A?@B@@A7>?>@B>?:787	BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL	MD:Z:100	RG:Z:0	BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:48:9218:101715#0	1024	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	46589@@AA>@>>>@ABB??>B??DBBB>B??7@BB@C@@BB?@B?@BBB?A??D?>@@BA@EB@@?@@@>A??=@A@D@>B@@B@A>+?@??C=@;8:=	BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL	MD:Z:100	RG:Z:0	BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:64:4088:153434#0	1024	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	46589@@AA>@>>>@BBB?@>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@@?D@=@AB@@CA?@?@@@>A?>>@A?DA>B?@B@A=7??=@@>:;88:	BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL	MD:Z:100	RG:Z:0	BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:25:6245:195434#0	16	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	><:<>BBB?DAAABCBCEB?BF@@CCC?CE@8ACCAAEACCEACEACCBEAC??C@@@@@>?BD@>C@D@AC@@D@@AB>BDABDA@7@D?=?<>92234	BD:Z:KNIJLBMMMMJJMNLBJIMLMMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKINNOONPOJMJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQMNMGOQQQLLPRQIONQPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOMOOLOQMPOOPOPQPQQOMPOOQQQRJONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:3:18284:27273#0	1040	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	=<<=>CCB@C??>@BCBDB@BD?A@B@>BE?7@@@A?DACCE?CD@CC2D@C@@C?BA@?>?BEA?CAE?@C=@C;@AB?ACABEA@8AD@=>=>;3123	BD:Z:KNIJLBMMMMJJMNLBJIMLMMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKINNOONPOJMJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQMNMGOQQQLLPRQIONQPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOMOOLOQMPOOPOPQPQQOMPOOQQQRJONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:6:8671:12086#0	1040	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	=<:=>CCB?C??>@BBCEA?BD?@C@>6@B<7@CBB?D@>CB;CE@CC@D>C?AC?@B@??@BD@>C@D@@C=@C@AAB?@E?AC@@7?D>=><>;3123	BD:Z:KNIJLBMMMMJJMNLBJIMLMMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKINNOONPOJMJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQMNMGOQQQLLPRQIONQPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOMOOLOQMPOOPOPQPQQOMPOOQQQRJONN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:42:8160:116894#0	1040	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	;;9;>AB@>C@>AABBAD@:AD??BA@?BD?5@BBA@E@BBD?BC@BBBDAB??C@@@@@>?BD@?C@D@AC@@DA@?B>BDABDA@7@D?=?<>92234	BD:Z:JMIILCLNNMKKMMKAIILLMMNKAJLLMMMKKAJKNIKAIIJIIKA@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLLKHMNNNOOOJMII	MD:Z:100	RG:Z:0	BI:Z:NPMMMGNQRRKKPQQHOMPPQPQPGOPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPQRRJOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:62:2822:138861#0	1040	chr3	58125525	60	100M	*	0	0	TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA	;:9;=ABB?A@@A>BBBD@9BB??@@A?@D?7@CB@@D@ABD?BC@BBBB@C??C>@@@@??BD@>B@D@@C@@D@??B?@D@ACA@8@D>=?<>92234	BD:Z:JMIILCLNNMKKMMKAIILLMMNKAJLLMMMKKAJKNIKAIIJIIKA@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLLKHMNNNOOOJMII	MD:Z:100	RG:Z:0	BI:Z:NPMMMGNQRRKKPQQHOMPPQPQPGOPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPQRRJOMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:23:11954:92523#0	0	chr3	58125526	60	100M	*	0	0	TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA	4584?>@A=@>>>ABBB?@?B??EBBB>B??7@BBAD?@BC?AC?ACCC?A@@E@?ABDB?EC@@??A@>A@>B@A@EC?CAAB@A?7AA@?C?B?:;@>	BD:Z:JJLILMDMMNNJIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLLLNNMNNMLMIOOMNMOMJMJ	MD:Z:100	RG:Z:0.1	BI:Z:NNPMOOIPPQOJJPPOHNMPOPPONGOPOPQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPRRQRRQPRNRQQPOOOKQN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:28:10887:136134#0	1024	chr3	58125526	60	100M	*	0	0	TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA	4584=>@A=@>>>@BBB?@>A??DBBB?B??7@BBAC?@BB?@B?@BBB?A@?D@?@ABA@DC?@?A@??A??7@A@DA?B?@B@@@8@?>@B?A>;;?=	BD:Z:IIKHMLDLMNMJJKKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNMNMLLJJLLMNILMNIMKLMLIMMIMLLNMLNMMLMJOONNNNLJLI	MD:Z:100	RG:Z:0	BI:Z:MMPLNPIPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOONPQMQPQQQQQQQOPNRQQPPNOJPN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:46:9571:119534#0	1024	chr3	58125526	60	100M	*	0	0	TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA	4564=>@A=@>>>@BBB??>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@??D@>@@CA@DC?@>@@?>A@>=@A@BB???@B?B@8@?>>C>A=98;9	BD:Z:IIKHMLDLMNMJJKKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNMNMLLJJLLMNILMNIMKLMLIMMIMLLNMLNMMLMJOONNNNLJLI	MD:Z:100	RG:Z:0	BI:Z:MMPLNPIPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOONPQMQPQQQQQQQOPNRQQPPNOJPN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:7:7761:36811#0	1024	chr3	58125526	60	100M	*	0	0	TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA	3375>>?@=?<:<8?AA=?>??=BABB;A@?7@BB>A?AB?=????@@@?A@?C@>@@B@>CB@@?@>@>A@?9@A?D@>9@AA:A>5@-<<@=>=97;9	BD:Z:IIKHMLDLMNMJJKKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNMNMLLJJLLMNILMNIMKLMLIMMIMLLNMLNMMLMJOONNNNLJLI	MD:Z:100	RG:Z:0	BI:Z:MMPLNPIPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOONPQMQPQQQQQQQOPNRQQPPNOJPN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:8305:80246#0	16	chr3	58125526	60	100M	*	0	0	TCACAAATAGGAGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA	8::;>AA?B=?/>ACCD@?@B>?@@:?BD?7@BB@@D;@BB=AB>BCBD@C??C>?A?>>@BD@>C@D@>C=@D??=B?@D@BD??8@D>=?>;=82353	BD:Z:MIIKBLNNNMKKNKAIILLMMNKAJLLMMMKKAJKNIKAIIKHIKAAHJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKKHMMNNMONNMKII	MD:Z:11G88	RG:Z:0	BI:Z:PMMOENPQRQOOQQHOMPPQPQPHNQOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPORRLOOMM	NM:i:1	AS:i:95	XS:i:19
HWI-ST115_0148:1:41:5881:8051#0	0	chr3	58125527	60	100M	*	0	0	CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	4848=>@=?>>>@BBB?@>B??DCCB>B??7@BBAC?@BB?AB?@BCC?A??EA>ABDBAECAA?@AA>A@?@@@@DB?BA@B@A@5A@?=A>A3=:=;<	BD:Z:JJIIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB	MD:Z:100	RG:Z:0.1	BI:Z:NNMMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:43:18815:159969#0	1024	chr3	58125527	60	100M	*	0	0	CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	483:;?@=@>=>@BBC???B@?DACB?B?@7@BBAC?ABC?@B?ACBC?@??E@>A@C@ABB@A?AA@?A@>:@A@FB?BA@B@A=7A@?8>0<1<;<>;	BD:Z:JJIIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB	MD:Z:100	RG:Z:0.1	BI:Z:NNMMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:45:18848:163363#0	1024	chr3	58125527	60	100M	*	0	0	TACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	4248;>@=?>>>@BBB?@>B??DBCB>B??7@BBAC?@BB?@B?@BBB?A@?EA>ABCB?DC@A?AA@?A@??A@@DB?BAABAA@5A@??B?B?>;=>=	BD:Z:JJMIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB	MD:Z:0C99	RG:Z:0.1	BI:Z:NNPMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH	NM:i:1	AS:i:99	XS:i:19
HWI-ST115_0148:1:62:10186:37536#0	1024	chr3	58125527	60	100M	*	0	0	CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	3739>=?===99-<;??=<=?4C>BB>B??7ACA@@@ABCA@B@A>?<9@?>C>.;;>:9=B@A??;@=A@?;?@:B?<?@???@94<@7>B.@>::>3<	BD:Z:JJIIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB	MD:Z:100	RG:Z:0.1	BI:Z:NNMMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:7:16791:111675#0	16	chr3	58125527	60	100M	*	0	0	CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	;;:?BB>DAAA@BCCEA?CE@@CCC?CE?8ACCAAEACCFACEACCCEAD??C@@A@@<?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@=A>:73664	BD:Z:IJKALMNMKKMNLBJIMLMNNJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMNNNMNNMPKAJJ	MD:Z:100	RG:Z:0.1	BI:Z:MNOHMOPQLLPRQIONQQRPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPNLPPMQOOPOPPOQQOMPOOQPPQKQOHNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:25:19405:133414#0	1040	chr3	58125527	60	100M	*	0	0	CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	685<=@?B>>89BBBC??A>>>><??AC?7=BB@@D@B@D@CD@CBBD@B??B@@?=A8<?D@?A>D>@B>?C;?>=<4@?@B>?8?D==>=@<:85543	BD:Z:IIKAKNNNLKMNLAIILLMMNKAJLLMMMKKAJKNIKAIIKIHKAAIJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJHMMMNMMNMQKAII	MD:Z:100	RG:Z:0	BI:Z:MMOGLPPQLLPQQHOMPPQPQPHOPPPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPRLQOGMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:42:13567:43693#0	1040	chr3	58125527	60	100M	*	0	0	CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA	999=?A?B>@>@BBBD@?BB?@BBB?BD?8@BB@@D@CBD@BD@BBBD@C??B@@A@@>?@C@?C@D@@C@@B?@?B?BC@BD@@8?D?=?=>>:73554	BD:Z:IIKAKNNNLKMNLAIILLMMNKAJLLMMMKKAJKNIKAIIKIHKAAIJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJHMMMNMMNMQKAII	MD:Z:100	RG:Z:0	BI:Z:MMOGLPPQLLPQQHOMPPQPQPHOPPPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPRLQOGMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:46:19063:128461#0	0	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	4486;><?=>>@BBB?@>B??DBBB>B??8@BBAD?@BB?@B?@BBB?@@@DA>ABCBAEC@A?@A@>A@>@@A@EC?CAABAA@7AA??C?C?@?@<>;	BD:Z:JJIJEONOOJJLLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKNNMNNMLMINNMMMPNKMJBK	MD:Z:100	RG:Z:0.1	BI:Z:NNMNJQQQOJJPPOHONPOPPOMGPOOQPMQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQQRRQPRNRQQQQQNIQNHO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:48:14037:187991#0	1024	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	3488<><>====ACC;;<=>?CC@A?B@?7@CC@CA@CCAACA@CCCAA>?D?+A@C?>D?@>;>@A=@?.8/96A==@=A?<A@7???>C?B?:=@<>9	BD:Z:JJIJEONOOJJLLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKNNMNNMLMINNMMMPNKMJBK	MD:Z:100	RG:Z:0.1	BI:Z:NNMNJQQQOJJPPOHONPOPPOMGPOOQPMQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQQRRQPRNRQQQQQNIQNHO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:67:3978:70373#0	1024	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	4486;><?=>>@BBB???B??DBBB?B??7@BBAC?@CC?@B?ABBC?A@@F@>A@CB?DC@A?AAA>@@?@A@@EB?BAAC@A@7@A??C?C@?=8=>:	BD:Z:JJIJEONOOJJLLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKNNMNNMLMINNMMMPNKMJBK	MD:Z:100	RG:Z:0.1	BI:Z:NNMNJQQQOJJPPOHONPOPPOMGPOOQPMQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQQRRQPRNRQQQQQNIQNHO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:26:3317:7675#0	1024	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	2/84:809869:<5=>?<;>=BA=A;>==6???>A@@B?AAAA>B?@@B9>B>;?@A@?A@@?<?@@=A?:AAB@;@><=<BAB%%%%%%%%%%%%%%%%	BD:Z:IIIIENONNKJKLJ@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMMLMJILLMNILMNIMKLMLIMMIMLLNMLNMMKLJONMNNONKLIAK	MD:Z:100	RG:Z:0	BI:Z:MMLNIRQPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPQLQPQQQQQQQOPNQPRRQPOHPNGN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:27:5617:148989#0	1024	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	4486;><?=>>?BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@>@ACA@DB?A>@@@>@A>A@A@EB>B?>B@@@7@@?@C>BA?>>=89	BD:Z:IIIIENONNKJKLJ@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMMLMJILLMNILMNIMKLMLIMMIMLLNMLNMMKLJONMNNONKLIAK	MD:Z:100	RG:Z:0	BI:Z:MMLNIRQPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPQLQPQQQQQQQOPNQPRRQPOHPNGN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:4:19975:33455#0	1024	chr3	58125528	60	100M	*	0	0	TCAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGAGCCTATCCCAAAC	4586;=<?=>>@BBB??>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?A>@@@>AA>9AB@DB?B?<A@B@81@==?=>=<<><<9	BD:Z:IIKIENONNKJKLJ@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMMLMJILLMNILMNIMKLMLIMMIMLLNMLNMMKMMPNMNNONKLIAK	MD:Z:0A85T13	RG:Z:0	BI:Z:MMPNIRQPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPQLQPQQQQQQQOQPRPRRQPOHPNGN	NM:i:2	AS:i:94	XS:i:19
HWI-ST115_0148:1:44:8541:127472#0	16	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	:;=?@>CA???BCCDA?CD@?CCB?CE?7ACCAAEACCEACE@CCCEBC@?C@@A@A>?BD@>C@D@AC@@D@@?B>BDABDA@7@D?>@>A?<:86644	BD:Z:JKAKMMNKKNNLBJIMLMNNK@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMNNLNMLPNAKJJ	MD:Z:100	RG:Z:0.1	BI:Z:NOHONOPLLPRQIONQQRQQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOLOQMPPOPOPPOPQOMPOOQPPPKPQHNNN	NM:i:0	AS:i:100	XS:i:19
HWI-ST142_0240:1:68:18974:109354#0	1040	chr3	58125528	60	100M	*	0	0	ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC	89;4?=A==>*?B@A2/=?(.416;-::1/AB@?B,9=B<4147/>8>A;:A98?<?(=?C@?B?D@@B>@A@@?B?BB@BD??8>C:<<>??;;86523	BD:Z:IKAJMNNLLMNLBIILLMMNKAJLLMMMKKAJKNIKAIIKIIJAAIKILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGMMMMMMLMPOAJII	MD:Z:100	RG:Z:0	BI:Z:MOGNNPPKLQQQHOMPPQPQPHOQOQOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPLQQGMMM	NM:i:0	AS:i:100	XS:i:19
HWI-ST115_0148:1:25:1660:58913#0	0	chr3	58125529	60	100M	*	0	0	CTAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATATGATGACGTGCCTATCGCAAAAA	3729<<?==>@@BCA9;;=8DA=A<B@?7ACA@C@@CC?AC?ACCC<A??E?<@@C??DB@A?@A@>A??%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJLJOOPOKJLMJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMIIKLHLKKMNMNNMLMINNLMMONOOJBBB	MD:Z:1A68T22C4C1	RG:Z:0.1	BI:Z:NNPNRQROJJPPOHONQOPPOMGOPOPQMPNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOJJPPMQPPQQPRRQPRNRQQQQROONNHHH	NM:i:4	AS:i:86	XS:i:20
HWI-ST115_0148:1:27:6860:74605#0	1024	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	3768=:<;=>>>BB@<>A@?ECAB?BA@8@CC@CA@CC@@C?@DCC?@@?EB<@AEA>DB@A?@@@=@@>9?9>@B?B@ABA==5A@=<B;B@?=D?>;>	BD:Z:JJJBOOPOKJLMJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMNMNNMLMINNLMMOOKNJBKI	MD:Z:100	RG:Z:0.1	BI:Z:NNNHRQROJJPPOHONQOPPOMGOPOPQMPNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPRRQPRNRQQQQROJONHOM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:26:3714:23222#0	1024	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	4766;:>==>@BBA??>A??DBAB=B@=86BA@C@@BB?@B?@BBBA@@?A@?A@BA>DB@@?@@@>A@<=<A>CA?B?@<?A57;?>>A=B)?=2><6:	BD:Z:IIIAOOPNKKKLJAJHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLLMJJKLMNILMNIMKLMLIMMIMLLNMLNMMKLIONMMNONLMIAKI	MD:Z:100	RG:Z:0	BI:Z:MMNGRQROJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPMPPQQQQQQQOPNQPQRRQOJNNGNL	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:27:16183:58921#0	1024	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	4867;;>===@@BA??>@??DBAB><@?8@BB>B?@BB?@B?@BBB?A@>D?>A@C@>BAA@??@?>B?>8)9=>A?A=A9AA@5;@=>@>B@==A>;79	BD:Z:IIIAOOPNKKKLJAJHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLLMJJKLMNILMNIMKLMLIMMIMLLNMLNMMKLIONMMNONLMIAKI	MD:Z:100	RG:Z:0	BI:Z:MMNGRQROJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPMPPQQQQQQQOPNQPQRRQOJNNGNL	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:4:9939:78105#0	1024	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	4856;;====@ABB???A??DBBB>B@?7@BB@A?@BB?@B?@BBB?@@?D@>@ACA@DB?@?@@??A@<>A??AB?B?A9?@@8>@?<?>B>:=@=;7:	BD:Z:IIIAOOPNKKKLJAJHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLLMJJKLMNILMNIMKLMLIMMIMLLNMLNMMKLIONMMNONLMIAKI	MD:Z:100	RG:Z:0	BI:Z:MMNGRQROJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPMPPQQQQQQQOPNQPQRRQOJNNGNL	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:2:3326:122977#0	1040	chr3	58125529	60	6S94M	*	0	0	CGATCTCAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC	%:<>=A?B@@=B>>:?BBC?9=?B<BC<B=@D<7<CCA@E<?CD<>><@?CC@A?@@?<??A>ABE=?C>E?@@?@E@@A>=?A8<D>=6=@=;;98523	BD:Z:LNMKMOMBLMLLJJMNLBJIMKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMMLIOOPQOMLJJ	MD:Z:94	RG:Z:0.1	BI:Z:OQPONPOIPQQQLLPRQIOMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMPNNONMOPMPOOPPQQPQQOMPOPRRPPNN	NM:i:0	AS:i:94	XS:i:19
HWI-ST115_0148:1:2:7831:17632#0	16	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	;>;>=C<>?;BA?D@?BD=@<8A/@D?7@A,A>D@@BC4:??CBBE@??AA=>;=A>A>C@?A@D?@C?@A=>@A>BB@BA??4;D<;=<A<<;<>8433	BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:43:1461:170805#0	1040	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	;><>=C=>?@@2CFA?BE@@CBA?BE?7@BCAAE@CBE@CD@CCCEBD?AC?@??A>?BE@>C@E?AC@@EA@@B?BEABD@@8@D?=@?B?=<;;6433	BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:5:8019:89787#0	1040	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	;>=>=C??>@@A<D5?BD?@CBB?BD)7@CCBAE@@AA@CD@CCBE@D?@C?AA?A>ABD@>CAD?AC@@DA@?B?BE?BEA@7@E?=A?B@=<;=6434	BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:63:8805:141247#0	1040	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	;>=>>CAA?BCCCEA?BE@@CCC?CE?8ACCAAEACCEACEACCCEBD?@C@@@@A>?BD@>C@D@AC@@DA@?B?BEABDA?7@D?>@>B?=<;;6434	BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:7:2654:174038#0	1040	chr3	58125529	60	100M	*	0	0	CAAATAGGTGATCTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGCCATATGATGACGTGCCTATCCCAAACA	%%%%%%%%%%%%%%%?BD?@?9@>AB=63@39:-./;D;BB>:BB=:><>@';>=A9>BD>>C@E=8B:?A9'7=:<>;:C??6?=8:<8>6:<::9532	BD:Z:KAKLMMOJMOMKOIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHMMLMKKLKMLKMLKHMMMNLMMKONDKJJJ	MD:Z:8G3T59A27	RG:Z:0.1	BI:Z:OHOPNOONQRQPRNQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMONOPOPPOPPOPPOMPOOQPPPJPPJNNNN	NM:i:3	AS:i:85	XS:i:19
HWI-ST142_0240:1:2:17083:137881#0	1040	chr3	58125529	60	100M	*	0	0	CTAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	;7<=<A@>@ABBCD@?BC??BBB>CD@7>CB@@E@BBE@BC@BBBDAC??C@@@@A>?BD@>C@D@AC@@DA@?B>BDABDA?7@D?>@>B?=<;;6434	BD:Z:MJJLMNLLNNLBJILLMMNKAJLLMMMKKAJKNIKAIIKIIK@AIKJLMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLMMMLMLKPNEJIII	MD:Z:1A98	RG:Z:0	BI:Z:PPNPNPJKQRQHOMPPQPQPHOQPPPONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJQQIMMMM	NM:i:1	AS:i:98	XS:i:0
HWI-ST142_0240:1:42:16611:43608#0	1040	chr3	58125529	60	100M	*	0	0	CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA	4<<==A@@@ABBBD@>AD??B@A?BC@8ABB@@D@@BC@CD@BBBD@C?@B>@B@@=??D@?C@D>>C@@C?@>?=BB@@DA@8@D?>@>B?=<;;6434	BD:Z:KAJLMNLLNNLBJILLMMNKAJLLMMMKKAJKNIKAIIKIIK@AIKJLMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLMMMLMLKPNEJIII	MD:Z:100	RG:Z:0	BI:Z:OGNPNPJKQRQHOMPPQPQPHOQPPPONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJQQIMMMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:23:11369:77134#0	0	chr3	58125530	60	100M	*	0	0	AAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAG	46667<<==@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ABA@DB?@>@@@>A?>@@A@CA?B?@B=?@7@@?@B<A@>@B@=;;6	BD:Z:IIAKPPPKKLLJAKHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKMJJLKMNILMNIMKLMLIMMIMLLNMLNMMKLINNMMMONLNJAKIN	MD:Z:100	RG:Z:0	BI:Z:MMGPQRQJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLQOQQQQQQQOPNQPQQRRPJPLGNLP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:65:18149:144308#0	1024	chr3	58125530	60	100M	*	0	0	AAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAG	46668<<==@BBB?@>B??DBBB>@??8@BBAC?@BB@@B?@BBB?@@?D@>@@CA@DA?@>A@?>A?>@@A@CB?B?@B;A@7@@?A@=BA@@B@=:>9	BD:Z:IIAKPPPKKLLJAKHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKMJJLKMNILMNIMKLMLIMMIMLLNMLNMMKLINNMMMONLNJAKIN	MD:Z:100	RG:Z:0	BI:Z:MMGPQRQJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLQOQQQQQQQOPNQPQQRRPJPLGNLP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:64:2167:161953#0	0	chr3	58125531	60	100M	*	0	0	TATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGT	42629:<=>BBB?@?B??EBCB<B??7@CB@C?@BC?@C?@CCC?A@?C?>@@C@ADBAA??A@>A??>A?@9B;AAAB@?37;B?>B:A>9>C.@;>6;	BD:Z:JJLLQQLKMMKAJILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLNNMLMINNLLLONKOKCKION	MD:Z:0A99	RG:Z:0.1	BI:Z:NNPPSPKJPPOHONQPQPOMGOOOQPMQMGOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQQPRNRQQQQRPLQMFOMQQ	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:44:10831:41289#0	16	chr3	58125531	60	100M	*	0	0	AATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGT	;<8=<?=@BBBD@?BD??B@B?BB?8@BBA@D?BBC@BC@BBCD@B>?C@@A@@>?@D@?B@D@@C@@C@@?B?BD?BD??8?D?=@>B@<<>?>:6623	BD:Z:JLLLKLNOMBJJMLMMNKAJLLMMMKKAJKNIKAIIKIIKAAHKJMNLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLMLLKKNLDMMMLII	MD:Z:100	RG:Z:0	BI:Z:NPPPHJOQRIOMPPQPQPHOQPQPOONGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOIOOPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:4:12425:123146#0	1040	chr3	58125531	60	100M	*	0	0	AATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGT	7=9>>=@AAABD@>BD??BBA?BD>7=BBB?D@BAD@BD@BBBD@C??C@@A@@>@BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>=>@>93744	BD:Z:JLLLKLNOMBJJMLMMNKAJLLMMMKKAJKNIKAIIKIIKAAHKJMNLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLMLLKKNLDMMMLII	MD:Z:100	RG:Z:0	BI:Z:NPPPHJOQRIOMPPQPQPHOQPQPOONGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOIOOPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:2:16506:172864#0	0	chr3	58125532	60	100M	*	0	0	ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCTAACAGTC	342688.<=<;;;4>@=ABB>=>@=6?BA=B@@B=@@@>?BABA<=>D>?A@C@=B?A@>>@@<A@>@@>;C6>?8;;>9*-7415>,58=>06@<>988	BD:Z:IILMQMMLMKAKILKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIJLLMMILMNIMKLMLIMMIMLLNMLNMMKLINMLMMNMLNNLLINNM	MD:Z:92A7	RG:Z:0	BI:Z:MMOPQLLPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOQPQQQQQOPNQPQQQQQLQPNLLPQP	NM:i:1	AS:i:95	XS:i:0
HWI-ST142_0240:1:68:15909:199980#0	1024	chr3	58125532	60	100M	*	0	0	ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC	46257:<?ABB?@>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@?@A?>A@>?@A@DB>B@@B?@@7???@C?B@@>=BA>?::9	BD:Z:IILMQMMLMKAKILKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIJLLMMILMNIMKLMLIMMIMLLNMLNMMKLINMLMMNMLNKCLINNM	MD:Z:100	RG:Z:0	BI:Z:MMOPQLLPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOQPQQQQQOPNQPQQQQQLQNGLLPQP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:65:7341:14563#0	16	chr3	58125532	60	100M	*	0	0	ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC	;:9;@?ABCCEA?CE@@CCC?CF@9ACCA@EACBEA@EACCCEBD@@D@AA@@>?BD@>C@D@AC@@D@@?B>BDABDA@7@E?>@>B@>>>A@<87444	BD:Z:LLLJKNPMCKIMLMNNKAKLLLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLLJMLBMMQLMJJ	MD:Z:100	RG:Z:0.1	BI:Z:PPPKINQQIONQQRQRPIPPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNPOPPOQPOPPNLOOOQPPPJOOHOORPPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:68:10363:186753#0	1040	chr3	58125532	60	100M	*	0	0	ATAGGGGATTCGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC	<:>;>=?BCA5@?BE?@CCB?@E?7@CCA@D@@CD>;D@CB=D@C?AC=@@@A>ABE@?C@E?@C?@E@@@B?BC@AE?@7@D>=>?A@<=@A@<;8343	BD:Z:LLLJKNPMLOLMLMNNKAKLLLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLLJMLBMMQLMJJ	MD:Z:10T89	RG:Z:0.1	BI:Z:PPPKINQQPRPQQRQRPIPPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNPOPPOQPOPPNLOOOQPPPJOOHOORPPNN	NM:i:1	AS:i:95	XS:i:0
HWI-ST142_0240:1:21:15621:181493#0	1040	chr3	58125532	60	100M	*	0	0	ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCTAAACAGTC	77<;;=A?BBD@?BD??BBB?BD?7@BBA@D@BAC@CB?BBABAC?@B@@A>A>A@B@>C@D>AC@@D@@?B?BAABC@?8@D=>??@A=?<A@<87334	BD:Z:LLLJKNOMCJJMMMMNKAJLLMMMKKAJKNIKAIIKIIKAAIJJMNMKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLLLKMNKBMLQLMII	MD:Z:91C8	RG:Z:0	BI:Z:PPPJHOPQIPMPPQPQPHOQPQPPNOGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPOPPGOORPPMM	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:25:4337:107457#0	1040	chr3	58125532	60	100M	*	0	0	ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC	=9=;>=ABBAD@?BD??BB<?BD?7@BCA?D@C=D@CE@BBBDAC?@B@@A@@>@AD@=C@D@AC@@D@??B>BDABDA@7@D?>@?A@>>>A@<87444	BD:Z:LLLJKNOMCJJMMMMNKAJLLMMMKKAJKNIKAIIKIIKAAIJJMNMKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLLLKJNLBMLQLMII	MD:Z:100	RG:Z:0	BI:Z:PPPJHOPQIPMPPQPQPHOQPQPPNOGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGOORPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:6:20790:48630#0	1040	chr3	58125532	60	100M	*	0	0	ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC	79<;<=@?@AD@?@D??BB?=BD?7?BBA?C>CBC?@A>CABC=B?@B>@A@A>?@D@=B@D@@C@@D@@?B>BDABCA@7@D?>@>B?>=>A@<87444	BD:Z:LLLJKNOMCJJMMMMNKAJLLMMMKKAJKNIKAIIKIIKAAIJJMNMKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLLLKJNLBMLQLMII	MD:Z:100	RG:Z:0	BI:Z:PPPJHOPQIPMPPQPQPHOQPQPPNOGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGOORPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:48:10313:163996#0	0	chr3	58125533	60	100M	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC	42427:>AAB?@>B??DBCB?B??7@BB@A?@BB?@B?@BBB?B??E@>ABCB?DB@A>@A@?B==;B@?DB=@AB=:A?5A@??C?B@??CCC@B=;;7	BD:Z:JJNNMMNNLAKJLKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMMLMINNLLLNMKNKDLJONNM	MD:Z:100	RG:Z:0.1	BI:Z:NNQOLKQPOHONQPQQPMGOOOPPNPMHNNPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPORNRQQQQRPLROHNKQQQO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:61:13700:181997#0	1024	chr3	58125533	60	100M	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC	42427:>AAB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?B@?E@>@@CBACC@A?@AA=A@>@@A@DB?BAABAA@7AA?AC?B@A??@C@B<;;8	BD:Z:JJNNMMNNLAKJLKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMMLMINNLLLNMKNKDLJONNM	MD:Z:100	RG:Z:0.1	BI:Z:NNQOLKQPOHONQPQQPMGOOOPPNPMHNNPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPORNRQQQQRPLROHNKQQQO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:23:10492:110769#0	1024	chr3	58125533	60	100M	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC	42427:>AAB??>A??DBBB>B??8@BBAC?@BB?@B?@BBB?@@?D@>@@CA@DB?@?@@@>A@><@A?CB<A?AA@A?.@@?@<??@=@DBB?@;886	BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMKLINMLLMNMKNKCMJNNML	MD:Z:100	RG:Z:0	BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQOPNQPQQQQPLROGNJPQPO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:43:9276:78825#0	1024	chr3	58125533	60	97M3S	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACTTGCCTATCCCAAACAGCAC	42427:>AAB?>>B??DBB@>B??7@BBAB?@BB?@B?@BBB?@@?D>>@ABA@DB?@>@A?>B@>:?>?C@>@@B>@A32:=:=C=B>==BBB>%%%%%	BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMLIKNMLLMNMKNKCMJNOMH	MD:Z:80G16	RG:Z:0	BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQQOPQPQQQQPLROGNJPQOL	NM:i:1	AS:i:92	XS:i:19
HWI-ST142_0240:1:68:5786:48821#0	1024	chr3	58125533	60	100M	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC	42427:>?AB?>>A?=DBAB>B?>7@BBAC??BBA@B@ABBB??@?D@;@@C@@DB@@>@@@>B@>2A;>C>>@@AB@B@7@?>@B?C>@>B@C9@:955	BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMKLINMLLMNMKNKCMJNNML	MD:Z:100	RG:Z:0	BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQOPNQPQQQQPLROGNJPQPO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:8:20535:125701#0	1024	chr3	58125533	60	100M	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC	42427:>AAB>>>A??DBBB>B??7@BB?C@@@>@@A@?BBA?A@?D@>@ACA@DB?@>@@?>A@><?A@CB?B@AB?@@4@??@C?B@=>DBC?A:985	BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMKLINMLLMNMKNKCMJNNML	MD:Z:100	RG:Z:0	BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQOPNQPQQQQPLROGNJPQPO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:41:6715:44727#0	16	chr3	58125533	60	100M	*	0	0	TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC	8>97>@BC@DA?CF@@CCB?BD?7ACCA?EACCEABE@CCCEBC@@CA@A?<?ABE@?C@E?AD?@E@>@B?BDABDA@7@D?>>>B@>>?BA>=<5414	BD:Z:LLJJNONCKJMLMNNKAKLLMMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKJMKBLLQOMLJJ	MD:Z:100	RG:Z:0.1	BI:Z:PPKKMPPIONQQRQRPIPQPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQOOQOPQOPPNLONOQPPPJOOHNOQRPPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:26:15826:129753#0	0	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC	22269<?AA??:<><:;;>6:;53;;>:8?ACB@AC@AB?0:?;4C;9@?C@?DAA;>??</@?:8??9;A=<>@B@%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJNJMOOLBKJMKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLLMINNLLLNMJNKCMJPNNMJ	MD:Z:100	RG:Z:0.1	BI:Z:NNOJLQQOHONQPQQPNGOOOPPMQMGONONNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQNRQQQQRPLROIOLOQQOK	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:7:13526:193281#0	1024	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC	44227<@AA???B??EBBB>B??7@BBAC?@BB?@B?@BBB?@@@D@>@ADAAEC@@?@A@?A??>AA@DB?B@ACAAA8A@@AD?CB?AECCAC==::7	BD:Z:JJNJMOOLBKJMKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLLMINNLLLNMJNKCMJPNNMJ	MD:Z:100	RG:Z:0.1	BI:Z:NNOJLQQOHONQPQQPNGOOOPPMQMGONONNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQNRQQQQRPLROIOLOQQOK	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:23:17158:177951#0	1024	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC	44227<@AA>@>@??DBBB?B?>7@BBAC?@BB?@B?@BBB?A@?D?>@@C@@DB?@>@@?>A@>A@A@EA?@AAB@A@7;@?@C>B@@@CBB@C><985	BD:Z:IIMJNNOKBLILLNMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKLMNIKMNIMKLMLIMMIMLLNMLNMMKLINMLLLNMKMKCMKONMLJ	MD:Z:100	RG:Z:0	BI:Z:MMOJLRRNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPQPQQQOPNQPQQQQPKRPHNLNQPOJ	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:23:9097:197994#0	1024	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCA	44227<@AA?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@@CA@DB?@?@@@>A?>A@@@DB?B@AB?A@8@@?@;?B@>=DBB@B==88:	BD:Z:IIMJNNOKBLILLNMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKLMNIKMNIMKLMLIMMIMLLNMLNMMKLINMLLLNMKMKCMKONMLL	MD:Z:99C0	RG:Z:0	BI:Z:MMOJLRRNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPQPQQQOPNQPQQQQPKRPHNLNQPOP	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:45:14120:173983#0	1024	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCCAACAGTCCC	32158<<@@?><>;=CAAA>@@=6?AA@>@@BB@AB@@BBB@A@?D@>@@B@>DB@@?@@8>A?<8@<>CA?B@A?@A>6;A=>B=@,==)BB;;=;867	BD:Z:IIMJNNOKBLILLNMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKLMNIKMNIMKLMLIMMIMLLNMLNMMKLINMLLLNMKKNKMKONMLJ	MD:Z:90A9	RG:Z:0	BI:Z:MMOJLRRNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPQPQQQOPNQPQQQQPKLRONLNQPOJ	NM:i:1	AS:i:95	XS:i:0
HWI-ST115_0148:1:44:14880:140203#0	16	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC	=:9;??@CDA?CE@@CCC?CE@8ACCA@D@CCCACEACCCEBD@@DAAAA@>?BD@>C@D@AC@@DA@?B>BDABDAA7@D@>@>B@>>?BA?=?94224	BD:Z:LJJMOMDKJNLMNNKAKLLMNMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKIMKALKPOPLJJJ	MD:Z:100	RG:Z:0.1	BI:Z:PKKOOOHONQQRQRPIPQQQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPOPPPPPPPNLONNQPPPJOOHNNQQRPJNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:46:16430:16956#0	1040	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC	=:82>AC=D@=BEA@CCB?BD@7ACCAAE@CCDACD@CCBDBC@AC?@AAB=@BD@?C@C@AC@@E@@AB>BDABDA@7@D?=?>BA>>?BA>=?:6223	BD:Z:LJJMOMDKJNLMNNKAKLLMNMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKIMKALKPOPLJJJ	MD:Z:100	RG:Z:0.1	BI:Z:PKKOOOHONQQRQRPIPQQQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPOPPPPPPPNLONNQPPPJOOHNNQQRPJNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:64:9439:169187#0	1040	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCA	=;99@BCCEA?BE@@CCC?BE@7@CCAAEA@CEABEACCCEBC@ADAAAA@>?BE@?C@D@@C@@CA@?B>BD@BD@@7@D@=@>BA>>?BA?=?;6234	BD:Z:LJJMOMDKJNLMNNKAKLLMNMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKIMKALKPOPLMJJ	MD:Z:99C0	RG:Z:0.1	BI:Z:PKKOOOHONQQRQRPIPQQQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPOPPPPPPPNLONNQPPPJOOHNNQQRPONN	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:26:17469:23074#0	1040	chr3	58125534	60	100M	*	0	0	AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC	9769<@AAD@?BD>?@B>?@B>7?BBA@D?ABC?@D@BBBA@C>?A>@B@A>@<C??C@D@@B@?D@A@B>BDABA@?7@D?>@=@@>=?B>?=?94114	BD:Z:LJJLNMCKKMMNNNKAJLLMMMKKAJKNIKAIIKIIKAAIKJLNMLLLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKKJMKBKJPOQKJII	MD:Z:100	RG:Z:0	BI:Z:PJJONPGONQPQPQPHOQPQPPOOGOOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMRRRPJMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:67:21313:129109#0	0	chr3	58125535	60	100M	*	0	0	TGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT	4323;>@A>@>B??EBBB?B??7@CBAC?@BB?@B?@CBB?A@?E@>A@CB?CA@A??A@>B@??A@8AA:BABBA@@7AA?>C?B@??DCC@D=><93=	BD:Z:JJMJOPMBLJMLMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKMINNLLLNMJMKCLKPONMJL	MD:Z:0G99	RG:Z:0.1	BI:Z:NNOJRQPHONQPQQPNHOOOPPMPNGNOOMNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMRQQQQRPLROIPMPOQOKP	NM:i:1	AS:i:99	XS:i:0
HWI-ST115_0148:1:7:21244:71724#0	1024	chr3	58125535	60	100M	*	0	0	TGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT	4312;>@A>@?@@@EBBB?B??7@CBAC?@BB?@C?@CCC@A@?E?>AACB?BC@A??A@?B=?>@A@?B?@AB<AB97@A>>C?B>7>DCC@E<?<8:=	BD:Z:JJMJOPMBLJMLMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKMINNLLLNMJMKCLKPONMJL	MD:Z:0G99	RG:Z:0.1	BI:Z:NNOJRQPHONQPQQPNHOOOPPMPNGNOOMNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMRQQQQRPLROIPMPOQOKP	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:27:7230:150558#0	1024	chr3	58125535	60	100M	*	0	0	GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT	42229>@A>@>B??DBBB>B??7@BB@C?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>AA>@@A@DB?C@AB@@@7@??@C>?A>@DBB@D?>;;;9	BD:Z:IIJJOOMBLJLLNNLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKMNILLNIMKLMLIMMIMLLNMLNMMKLINMLLLMMKMJCMKPOMLJL	MD:Z:100	RG:Z:0	BI:Z:MMJJRRPGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPQPQQOPNQPQQQQPKQPIOLPOPOJP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:46:14388:190521#0	1024	chr3	58125535	60	100M	*	0	0	GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT	4222;>@A>@>B??DBBB>B??7?BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@??>A?>@?A@CC?C@B??A@7@=?@A>A@=>DBB@D>><;;;	BD:Z:IIJJOOMBLJLLNNLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKMNILLNIMKLMLIMMIMLLNMLNMMKLINMLLLMMKMJCMKPOMLJL	MD:Z:100	RG:Z:0	BI:Z:MMJJRRPGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPQPQQOPNQPQQQQPKQPIOLPOPOJP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:41:6701:64831#0	16	chr3	58125535	60	100M	*	0	0	GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCA	99.7=BCA@=;D?@C/B<BD?7<B@A@D>8CE@?E@CCCD@@?@@?@?=@>ABC@<C@B>=A<3B@?@>?@C<?A?>7@E=>>=@A<=?@B=>@:;4243	BD:Z:JJMNMCLJNMMNNKAKLLMNNKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILKAKKONPOJMJJ	MD:Z:99T0	RG:Z:0.1	BI:Z:KKOQNGNNQQRQRPIPQQRPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPPOQPOQPNLONNPPPPJOOHNNPQQRJONN	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:45:15962:97980#0	1040	chr3	58125535	60	13S87M	*	0	0	GCTCTTCCGATCTGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATA	8:;;?@>5@A?@D@>?@BABD@>BE?@BBA>AC?7:BBA@D@ABB@BB@BBBBAC?@B@=@@@><>D@>C?D@AC>@CA??B>BDABD??7?C9::2624	BD:Z:OLKJKMNMOMLOMJJLMKAIILLMMNKAJLLMMMKKAJKNIK@IIKIHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLMKMLLINPPQLLII	MD:Z:86C0	RG:Z:0	BI:Z:QOOOLPMORRPQQKKPQQHOMPPQOQOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOQQROPMM	NM:i:1	AS:i:86	XS:i:19
HWI-ST142_0240:1:5:19100:129082#0	1040	chr3	58125535	60	100M	*	0	0	GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT	988;>ABC??<D?@BBB<A;?8?BB@ACABAB@BB@BCCDAC>@C@?A@@>?BD@?C@B@AB@?C@@?B>BDABDA@7@D=>@>A@>>?BB?>?<;3244	BD:Z:JJLMLCKKNMNNOKAJLLMMMKKAJKNIKAIIKIIKAAIKJMMMLLMJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJJMKAKJNOPOJMII	MD:Z:100	RG:Z:0	BI:Z:JJOPNGNMQQQPQPHOQPQPPOOHNPPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPRRRJOMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:6:11384:77072#0	1040	chr3	58125535	60	100M	*	0	0	GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT	387:=AAB@?BD>@BBB>AA?5=CBA?B?@CD@@D@BBBD@B?@B?=@?@>AAC?=B=B>>B>?C8>?A=?A=?@;=6;@=<==>@9<=>>>><;<6123	BD:Z:JJLMLCKKNMNNOKAJLLMMMKKAJKNIKAIIKIIKAAIKJMMMLLMJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJJMKAKJNOPOJMII	MD:Z:100	RG:Z:0	BI:Z:JJOPNGNMQQQPQPHOQPQPPOOHNPPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPRRRJOMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:26:7487:94776#0	0	chr3	58125536	60	100M	*	0	0	TGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	4324<>@>??B??DBBB>B??7ABB@CA@CB?@C?@BBC@AA@DA>@AC@?EB?@?@@@>A@?>A@:1B?@@ABAA<6?@?BE?B@2?ABC@D9@>;9>;	BD:Z:JJMLPNCLKMLNMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLINNLLLNMJMJCLJQOOMJLJ	MD:Z:0G99	RG:Z:0.1	BI:Z:NNOPRPIONQPQQPNHPOOPPMPMHNNPMMHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQQQQQRPLROIPNQPOOKPN	NM:i:1	AS:i:99	XS:i:0
HWI-ST115_0148:1:42:20655:156999#0	1024	chr3	58125536	60	100M	*	0	0	GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	4218;>@>?>B??DBBB;BA?7@BBAC?@CB?@B?@BBB?@@?D@>@@C@ADC@A?A@@?A?>;@A@DB?B@AB@A47A;=>B;?@?>BCC@A>A>;8>>	BD:Z:JJJLPNCLKMLNMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLINNLLLNMJMJCLJQOOMJLJ	MD:Z:100	RG:Z:0.1	BI:Z:NNJPRPIONQPQQPNHPOOPPMPMHNNPMMHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQQQQQRPLROIPNQPOOKPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:6:17640:16154#0	1024	chr3	58125536	60	100M	*	0	0	GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	4226;>@>?>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@@?E@>@ACAAEC@A?AA@?A???AA@DB?BBAB@A@7@@??C?B@??BCC@E??@=:>>	BD:Z:JJJLPNCLKMLNMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLINNLLLNMJMJCLJQOOMJLJ	MD:Z:100	RG:Z:0.1	BI:Z:NNJPRPIONQPQQPNHPOOPPMPMHNNPMMHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQQQQQRPLROIPNQPOOKPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:44:21041:193043#0	1024	chr3	58125536	60	100M	*	0	0	GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	4224;>@>?>A??DABB?B??7@BB?C?@BB?@B?@BBB?@@?D@>@@C@>DB?@?@A?>A@>;@?>BB>AAA@@A@7A@9@A?B@>@C=B=A>?=9;9;	BD:Z:IIJKPMDLJMLNNMH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLNILMMIMKLMLIMMIMLLNMLNMMKLINMLLLMLKMJBMKPPNLJLI		MD:Z:100	RG:Z:0	BI:Z:MMJPRPIOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPQPQOPNQPQQQQPKQOIPMPQNOJPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:43:6266:2559#0	16	chr3	58125536	60	100M	*	0	0	GGGATGTGTATGCAGATATTCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGATGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	639=@'A==@A;:<*8.=5,4.>@?@B<?CC:79<A@9=<A;@B<<@?@<A>B@?;>A7:=-8@=A>=8@??=A9=45A0:1==>;,9@?85;6020752	BD:Z:JMNMJJKNMNNNNKNLLLKNLJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLMLHHKMIMKKLKMLKMLKHMMMMKLKILJAKJOMOOMNKJJ	MD:Z:5T8A4G41G38	RG:Z:0.1	BI:Z:KOQQKLMQQRQRQPRQQQPQOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOQQMMPQNQPPQOPQOPQNLONNPOPPJOOHNNPPQQLOPNN	NM:i:4	AS:i:80	XS:i:19
HWI-ST142_0240:1:1:5882:180128#0	1040	chr3	58125536	60	100M	*	0	0	GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	;8<=@AD@>BD?@BBB?BD?8@BBA@EABBE@BE@BBCDAB??C@@@@@>?BD@?B@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@>B><72464	BD:Z:JLMKBKKNNNNOLAJLLMMMKKAJKNIKAIIKIIKAAIKJMNLLLMKKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJIMKAJJNMPNNMJII	MD:Z:100	RG:Z:0	BI:Z:JOPPENLPQRPQPHOQPQPPOOHOOQLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPRRLOOMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:47:16817:196618#0	1040	chr3	58125536	60	100M	*	0	0	GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT	;;:=@AD@>BD?@BBB?BD?7@BBA@E@CBE@BE@BBCDAC??C@@@@@>?BD@>C@D@AC@@DA@?B>BDABDA@7@D>>@>B@>>?BB?>B><72464	BD:Z:JLMKBKKNNNNOLAJLLMMMKKAJKNIKAIIKIIKAAIKJMNLLLMKKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJIMKAJJNMPNNMJII	MD:Z:100	RG:Z:0	BI:Z:JOPPENLPQRPQPHOQPQPPOOHOOQLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPRRLOOMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:61:20673:123650#0	0	chr3	58125537	60	100M	*	0	0	GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC	4246;>=?=B??DBBB>B??7@BBAC?@BB?@B?@BCB?A@?D@>@ACA@EB@A?B@A?@@>?@A@DB?B@BB@B@7A@>?C<?@<>C9C@D==?<:==5	BD:Z:JJLMNDMKNLNNMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHNNLLLNMJMJBLJPPONJLJK	MD:Z:100	RG:Z:0.1	BI:Z:NNPPQIPNQPQQPNHPPOPPMPMGONONMGONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPQQQRPLROIPNRQPMKPNO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:47:16975:197510#0	1024	chr3	58125537	60	100M	*	0	0	GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC	3246;>=>>A??DBBA?B?=8?BA@C??BB??B@@@BB@@@?D?>?@C@@DA?@?.;<39@?>AB@BB?B8A>AA=1;?>>B?B@@@DBA@B9??;9;57	BD:Z:IIKLNDNJMMNNMI@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMILMNHMKLMLIMMIMLLNMLNMMKLINMLLLMLJMJBLKPPOMJLIJ	MD:Z:100	RG:Z:0	BI:Z:MMPPPIQMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPQPOPNQPQQQQPKQOHPNQQPMJPNO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:66:20946:104000#0	16	chr3	58125537	60	100M	*	0	0	GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC	1979?D=?BC>@B=B?BE?5?ACB@8*6&@@>B9CCCD@B@@C?A@?A>AAE@?C@D@@A@@DA@AB?BEABDA@7@E?>@>@@>>ACB@?B?>:85643	BD:Z:MNLBKJOMNONKAKLLMNNLKAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@KJNMNNMQKKJJ	MD:Z:100	RG:Z:0.1	BI:Z:OQPHLLPQRQRPIPQQRQQOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPPPPPPOLONNPOOPJOOHNNPPPQKQPONN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:8:18877:191709#0	1040	chr3	58125537	60	100M	*	0	0	GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC	577-9B>>BC=>:B??;D?2@CCB@C;=A?;A9=@CA9>C??C7@B>B>@BD><C<=;9?2:74<A>?AA>>A<=+<A=:=>AA==:AA?=A>>;:66.3	BD:Z:LMKAJKNNONOLBJLLMMMKKAJKNIKAIIKIIKAAIKJMNMKLMKLJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILKAJINMNNMQJJII	MD:Z:100	RG:Z:0	BI:Z:OPPGLLOPRQQPHOQPQPPOOHOPPMNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPRLQONMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:28:19470:70891#0	0	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	4466;;>=A??DBCB>B??7@BBAC?@BB?@B?@BBB?@@@D@>AACA?EB@A>?A?>@@?>@@@DB?B@BBAA@8@@@AC?B@?ADCCAE?AA?>?=;=	BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMNLLLNMJMJBKJPOPNKLJKL	MD:Z:100	RG:Z:0.1	BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNGNNNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPQQRPLROIPNRRQNIPNOP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:42:7431:10467#0	1024	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	4466;;>=A??DBBB>B??7@BBAC?@BB?@B?@BBB??@?D@>A@C@@DC?A?@A@>A??;@@@DB?B>B@AB?7A@>?C?B??ADCB@;8<=;===;=	BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMNLLLNMJMJBKJPOPNKLJKL	MD:Z:100	RG:Z:0.1	BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNGNNNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPQQRPLROIPNRRQNIPNOP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:65:1258:51190#0	1024	chr3	58125538	42	26M74S	*	0	0	GATTTGTATGCAAATATGCGTTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	3558====A@>C<C@;A><4>AB?%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIJJJJJJJJJJJKKKLKKJJJJ	MD:Z:26	RG:Z:0.1	BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNMMNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNOOOOOOOOOOOONMLNNNN	NM:i:0	AS:i:26	XS:i:0
HWI-ST115_0148:1:7:18508:91166#0	1024	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	3558><>=:?>BB@B>A??7@CC@C@9@C@@C@@CCC@A@?D??A@C@?4B??>?A@?????BA5DB?B@BBAA?7??9>C?B@??DCC@D>@>==?=;>	BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMNLLLNMJMJBKJPOPNKLJKL	MD:Z:100	RG:Z:0.1	BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNGNNNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPQQRPLROIPNRRQNIPNOP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:67:12142:176815#0	16	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	9=<5B2:,1125$?;@C:04BC?<D690D:@.9B<CC@C;@A=?A=@<@@@>=B=>7>?:;C=>>B>>A@>@??3<@?:=>;A;=>C@??B??<::8453	BD:Z:NLBJJNNNOOKAKLLMNNLKBJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JJNLNMLQNKNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPHNKOPRQRPIPQQRQQPOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQPOQPNMONNPOOOJOOHNNPPPPKPROQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:7:5473:177428#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	9==>B==0=>@<<B?@B96=CCA?D>CCD@5B?@@@D>A=@@:<<>A?A:A=;94823@)8:;@@<9AA=@@<>6<@5:=?B@=>@CB@>B><<::9423	BD:Z:NLBJJNNNOOKAKLLMNNLKBJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JJNLNMLQNKNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPHNKOPRQRPIPQQRQQPOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQPOQPNMONNPOOOJOOHNNPPPPKPROQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:28:9824:174688#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTAACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	69:=A???B><+@B??@>64B>A?@?AAC>AB=BC>C@A??A>4@:3>ABD@?C@@;;?:8>&>??<>C@A@3?6?B=>>>>@==?AA>?A>@:;96424	BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKKIIHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII	MD:Z:62G37	RG:Z:0	BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOOPMMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:43:5151:42131#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	5<:=B?>BD??BBB>@A=5$BB@@D@CBEACD@CBBD@C??B@@@@@>?BD@>C@D@AB@@D@@?B>BDABDA@7@D?>@?B@>>?BB@?C??<:96434	BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII	MD:Z:100	RG:Z:0	BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:46:16188:21914#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGGTGACGTGCCTATCCCAAACAGTCCCTTCA	9=;=B?>BD??BB??BD?7@BB@@E@ABD?BD@BBBD@C??C>@A@@>?BD@>B@D@AC@@DA@?B>BD@@DA@7@D?>@>B@>>?BB@?A??<:96434	BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLKKGJLKJGLLLLKKJILJAJIMMNLMPNJMII	MD:Z:70A29	RG:Z:0	BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPOLPPONLOOOPOPPJOOGMMPPPPLQQNPMM	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:46:5500:75404#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	99<=B??BD??BCB?BD?7@BB@@E@BBEABD@BBBDBC??B@@A@A>?BD@?C@D@AC@@DA@?B>BDABDA@7@D?>@>A@>>?BB@?C??<:96434	BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII	MD:Z:100	RG:Z:0	BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:46:9470:13130#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	<<==B?>BD??BCB?BD?7=BB@@E@CBEACD@CBBDBC??C@@A@A>?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C??<:96434	BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII	MD:Z:100	RG:Z:0	BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:7:12534:169939#0	1040	chr3	58125538	60	100M	*	0	0	GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA	7<<=B??BD??BCB?BD?8@BBB@D?CBDACC@BB@DBB??C@@@@A>?BD@>C@D@AB@?D@@?B?BD@BD@@7@D?>>>B?>>?BB@?A??<:96434	BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII	MD:Z:100	RG:Z:0	BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:62:21313:158611#0	0	chr3	58125539	60	100M	*	0	0	ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA	45668<<A>?DBBB?B??7@BBAD?ACB?@B?@BBB?A@?E@>@@CA@EB@A;@AA<B??AAA@D>?B@AB@A?5A=?>B?B>>?D@C?C==,<%%%%%%	BD:Z:JJKANMOMONNJAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMLLLNMJMJBKIPOOOKMJKLJ	MD:Z:100	RG:Z:0.1	BI:Z:NNOHQORPQQPNHPPPQPMPMGNNPMMHNMNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPQRPLROIPNRRROJNNOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:66:2007:42196#0	1024	chr3	58125539	60	100M	*	0	0	ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA	46688<<A>?DBBB>B??7@BBAC?@BB?@B?@BBB?A@?EA>AAC@@DB@@?@@A>A@>?A>@DB=B@@A@>@7@;;:@<=:=9DBB@B>99>7<=753	BD:Z:JJKANMOMONNJAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMLLLNMJMJBKIPOOOKMJKLJ	MD:Z:100	RG:Z:0.1	BI:Z:NNOHQORPQQPNHPPPQPMPMGNNPMMHNMNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPQRPLROIPNRRROJNNOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:8:8758:124039#0	1024	chr3	58125539	60	100M	*	0	0	ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA	35679==A>@ECCB?B?@8@CCAD?@CC?@C?@CCC?AA@EA>AADAADB?@?A@@>AA>?@B@DB?BABB@A=6A@>>C?B@ABDC<@E??@>=A?:?;	BD:Z:JJKANMOMONNJAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMLLLNMJMJBKIPOOOKMJKLJ	MD:Z:100	RG:Z:0.1	BI:Z:NNOHQORPQQPNHPPPQPMPMGNNPMMHNMNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPQRPLROIPNRRROJNNOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:21:16438:172597#0	1024	chr3	58125539	60	100M	*	0	0	ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA	3557:<9@>=B@@==?>=3?@???@@A@>@B>@BBB>A=>B=>?@B@=CA@==??@;?>>:?A>B4:947>@B?5@:9<@3?49=BAA8B%%%%%%%%%%	BD:Z:IIJAOLOMOOMIAKKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKMNIMJLMLIMMIMLLNMLNMMKLINMLLLMLJLIBLJOPONLMIJKI	MD:Z:100	RG:Z:0	BI:Z:MMNGQORPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPOONQPQQQQPKQOHOMRSQOJNNOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:22:4826:29353#0	1024	chr3	58125539	60	100M	*	0	0	ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA	46668<<A>?DBBB>B??7@BB@A?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>B@>:@A?D@?B@AA@A?7@??@B<B@>@BBB@B???>=?=7;9	BD:Z:IIJAOLOMOOMIAKKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKMNIMJLMLIMMIMLLNMLNMMKLINMLLLMLJLIBLJOPONLMIJKI	MD:Z:100	RG:Z:0	BI:Z:MMNGQORPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPOONQPQQQQPKQOHOMRSQOJNNOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:5:4421:176647#0	1024	chr3	58125539	60	100M	*	0	0	ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA	4666:<<A>?DBBB>B??7@BBAC?@BB?@B?@BBB?@@?C@>@@CA@DB?@>@@@>A@>@?A@DB>B@BB@A@7A??@B>B@>@CAC@D@@A>?A=<>9	BD:Z:IIJAOLOMOOMIAKKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKMNIMJLMLIMMIMLLNMLNMMKLINMLLLMLJLIBLJOPONLMIJKI	MD:Z:100	RG:Z:0	BI:Z:MMNGQORPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPOONQPQQQQPKQOHOMRSQOJNNOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:22:14446:26100#0	0	chr3	58125540	60	100M	*	0	0	TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGAGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	225:9::<297<<-0=;4;==:9;?9@@77?AB;:>A?<D;<<=@@>DBA%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJAKMPNOONJBKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMKKJLHLKKMMLMMLKLHMMKLLNMJMJBKIOOONLMKKLJK	MD:Z:59T40	RG:Z:0.1	BI:Z:NNHOPRQQQPNHPPPQQMPMGNNONMGOMMONNHHOQPRPQOPQONOQQQNQQQMOPPOPPOPMQPPQQPQQPOQMQPPPPRPLROIPNRRRPKOLOPNP	NM:i:1	AS:i:95	XS:i:19
HWI-ST142_0240:1:48:10504:20320#0	1024	chr3	58125540	60	100M	*	0	0	TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	3555::@><C?6?7>@>7@AA?A?@BB??B??B@B?@>>A?;?>?@?B?A?:>??<?>>26>4B9::<A=@A?68=-79>7>0:@A7>@488/8??;%%%	BD:Z:IIAKMOOOONIAKLKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLNIMKKMLIMMIMLLNMLNMMKLINMLLLMLJLIALJOOONLNJJKIJ	MD:Z:100	RG:Z:0	BI:Z:MMGOORRPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNPMQPQQQQPKQOHOMQSRPJPLOPNO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:8:20458:106737#0	1024	chr3	58125540	60	100M	*	0	0	TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	46639:@>>DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@:@ACA>DB?@(??@>B@?>@AACA?B>AA@A@7A?>>C?BA@@DBB@6<@>>>A@<=<;	BD:Z:IIAKMOOOONIAKLKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLNIMKKMLIMMIMLLNMLNMMKLINMLLLMLJLIALJOOONLNJJKIJ	MD:Z:100	RG:Z:0	BI:Z:MMGOORRPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNPMQPQQQQPKQOHOMQSRPJPLOPNO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:2:12658:158403#0	16	chr3	58125540	60	100M	*	0	0	TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	==@<=BE@@CCC=CE@8ACCAAFACCE@CEACCCEBD@ADAAAAA?@BDA>C@E@AC@@DA@?B>BDABEA@7@D?>@>BA>>?BB@?C@@==>>93674	BD:Z:BJIMMNPOLBKLLMNNLKBKKNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMLMLKOMNQKJJJ	MD:Z:100	RG:Z:0.1	BI:Z:HNMPNPPRPIPQQRQQPPIOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPPPOLOONPOOOINOHNNPPPPJOQPSONNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:47:4453:178967#0	1040	chr3	58125540	60	100M	*	0	0	TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	98=:<>B>?68;==B>26A7A;B?1AD?A?>A?B4<=:>9;>>?B7A@D@;7=C>4?:9>,7))/BA>=C?@38A:6:;-4;9<9?8>A>?<<<>=6553	BD:Z:AIILMOOPMBKMMMMMKKAJKNIKAIIKIIKAAIKJMNMLLMJLKMNLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IIMLMLKNMMQKIII	MD:Z:100	RG:Z:0	BI:Z:GNLOMPOQQIOQPQPPOOHOPQMOGOLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOQPRONMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:67:1442:112789#0	1040	chr3	58125540	60	100M	*	0	0	TTTGNATNNNAANATNCTTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	%%%%%%%%%%%%%%%%%%%%A@B?C<=+92=@5@B:@=AC;:,>91:73,=A:A;3</14.;4=3>799;;=55A:.>=4?=>?AA??A>@<<<>;7553	BD:Z:AIIIJKKKKJJJJIIIJAAJKNIKAIIKIIKAAIKJMNMLLMJLKMNLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IIMLMLKNMMQKIII	MD:Z:4T2G0C0A2T2G1G82	RG:Z:0	BI:Z:GNMMKMMNOONNNNNNPHHOPQMOGOLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOQPRONMM	NM:i:7	AS:i:83	XS:i:19
HWI-ST142_0240:1:68:10518:127054#0	1040	chr3	58125540	60	100M	*	0	0	TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG	=9<;=AD?@A<B?BD?7@B?@@D?BBC>AC@BBBCAC??C@@A@A>@@D?>C@D@@B>@D@A?B??D@=D@@8@D==>?@A>=?BB??C@@==>>93674	BD:Z:AIILMOOPMBKMMMMMKKAJKNIKAIIKIIKAAIKJMNMLLMJLKMNLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IIMLMLKNMMQKIII	MD:Z:100	RG:Z:0	BI:Z:GNLOMPOQQIOQPQPPOOHOPQMOGOLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOQPRONMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:25:1522:126422#0	0	chr3	58125541	60	100M	*	0	0	TTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGG	46367>=>DCBB?B?>7@CB@C?@AC@A=@@CBC?A@?D@>@@CA@EA?A?@@@?B??;@?@CA7>@@@@@@7?@?BC?B=>?CCC?3@??:>-B%%%%%	BD:Z:JJKJPOPOOJBLKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKLNMJMJBKIONONKNKLLJKN	MD:Z:100	RG:Z:0.1	BI:Z:NNONSQRQPNHPPPQQNPMGNNOMNGNNMNNNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQPLROIPNRRRPLPMMPNPO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:28:2058:137769#0	16	chr3	58125542	60	100M	*	0	0	TGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGT	>;:=B>@CBB6BD?4@CCB@E@CCE?>D@CACD@C?@A>@?@<>@BE@?C@E?@C>@E@@AB?AE@BE@@7@E?>?>?>>>ABB?A@?===?A>==8243	BD:Z:IMLMOOMBLMLMNNLKBKKOIJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLLJNLLPNMLMJJ	MD:Z:100	RG:Z:0.1	BI:Z:MPPQNPOIPQQRQQPPIPPQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQNLPNNQOOOINNGNNPPPPJOPORPPPONN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:63:2187:107403#0	0	chr3	58125543	60	100M	*	0	0	GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	44268;BAAB>B??7@BBAC?@BB?@C?@BBB?A@?E@=@@AA@EB?A?@?A>B@?8@@@DB=BB@BA@?7A@5?A?B@>>DCC?E@A@??CC@%%%%%%	BD:Z:JJMLQQPKCLLLMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMMJMJBKIONNMKMKMMKKNMI	MD:Z:100	RG:Z:0.1	BI:Z:NNQPSRQNHPPPQQNQNGNNOMMGOMMOMMHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKROIPNRRRPLQOOOLPOPM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:45:13340:71390#0	1024	chr3	58125543	60	100M	*	0	0	GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	44268;BAAB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@CB?@>@@?>@@>@@A@DB?B?@B?@?7@@?@D>BA@>DBB@D@@@>@BBAC@:867	BD:Z:IILLRQPJBLLLNNJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLKLMLHMMIMLLNMLNMMKLINMLLLMLJLIAKINONMKNKLMJJMLI	MD:Z:100	RG:Z:0	BI:Z:MMPPRRQNGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPPPQQQPKQOHOMQRQPLROOPLOOOM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:6:4941:69627#0	1024	chr3	58125543	60	100M	*	0	0	TTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	2227<76;;:<>@:3?A??A???A??>>?@BB?@@?A@>@@B>>DB@???A@>A?<9==:?=:5::>9>=7A@?9A=6>>0B@<<B<?9;>A@=%%%%%%	BD:Z:IIJLRQPJBLLLNNJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLKLMLHMMIMLLNMLNMMKLINMLLLMLJLIAKINONMKNKLMJJMLI	MD:Z:0G99	RG:Z:0	BI:Z:MMPPRRQNGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPPPQQQPKQOHOMQRQPLROOPLOOOM	NM:i:1	AS:i:99	XS:i:0
HWI-ST142_0240:1:23:14443:42600#0	16	chr3	58125543	60	100M	*	0	0	GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	;9<?=>BBB>>D@7@BB@@D@BBDABEACCBDAD??BA@A@@>?BD@>C@D@AC@@D@@@B>BDABDA@7@D?>@?B@>>?BB@?C@@>>@A?=><4484	BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII	MD:Z:100	RG:Z:0	BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:3:18249:30680#0	1040	chr3	58125543	60	100M	*	0	0	GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	99=?=?BBB?BD@7@BB@@>@BBD@CD@BBCC@D??BA@@@@>?BD@>C@D@AC@@D@@?B>BD@BDA@7@D?>@>B@>>?BB@?C@@>>@A?=><4484	BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII	MD:Z:100	RG:Z:0	BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:44:7743:26975#0	1040	chr3	58125543	60	100M	*	0	0	GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	;9=?=>BBB?BD?7@CB@@D@BBDABEACCCD@D??CA@@@@>?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@A?=><4484	BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII	MD:Z:100	RG:Z:0	BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:7:12525:180227#0	1040	chr3	58125543	60	100M	*	0	0	GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG	99=?=@@@@>>D?7=BB@?>@ABD@CD?BBCDAD?@B?@A@B>?BD@?C@C@AB>@D>??B>BD@ADA@7@D>>@>A@>>?BB@?C@@>>@A?=><4484	BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII	MD:Z:100	RG:Z:0	BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:22:11114:21452#0	0	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	42638@@AA>B??7@BBAC?@BB?@B?@BBB?@A?D@>@ACA@CB?@>@@A>A@>;AB@DB?BAAB@A@5@A>?C?B@?AECCAE?A@?ACC@BC>;;<9	BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIONNMJMKLNKLNMIM	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNRRRPLQOPPMNOPMO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:27:17514:154190#0	1024	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACACTCCCTTCGAGGGGG	32468@@?>=A?>7A>:>;?@A?@@A@ACCC@A>?C??@@C@?3B?:;;;>9A?<A@A?DB?545:@?0(@A*>B0A=:;-=5(8'93/7CB?%%%%%%%	BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIILKMJMKLNOMNJJJ	MD:Z:85G7A3T2	RG:Z:0.1	BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNNQOPLQOPOPNOJJJ	NM:i:3	AS:i:88	XS:i:0
HWI-ST115_0148:1:42:20622:45748#0	1024	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	42638@@AA>B??7@CBAC?@BB?@B?@BBB?@@?D@>@ADA@DB?@>A@@>AA??@A@DC?C@ABAA=7A@>?B?B@?AECCAA@A@?ACC@DA>:<;9	BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIONNMJMKLNKLNMIM	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNRRRPLQOPPMNOPMO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:61:15878:77743#0	1024	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	42638@@BA>B??7@BBAC?@BC?@C?@BBB?A@?E@>@ACA@DB?@?@@@>AA>@AA@DB?CAAC@B@8AA?9A>A<=>DCC@DAAB?AC@@>C>;<;9	BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIONNMJMKLNKLNMIM	MD:Z:100	RG:Z:0.1	BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNRRRPLQOPPMNOPMO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:28:17271:25147#0	1024	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	42638@@@A>B?>7@BBAC@@BA?@B?@BBB?A@?D@>@ACA@DB?@?@@?>A@>@?A@DB?B@AB@@@8@@?AC?B@@@ABA?B?A@>=@B@CA=99:5	BD:Z:IILNRPLBLMLNNKKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJLMLILMIMLLNMLNMMKLINMLLLMLJLIAKINNNMKMKLMKKMLIM	MD:Z:100	RG:Z:0	BI:Z:MMPPRQPGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOQPQQPKQOHOMQRQPKRPPPNMOOMO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:23:15894:66320#0	1040	chr3	58125544	60	11S89M	*	0	0	TCTTCCGATCTTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAATAGTCC	%:;==5>?B=C?B@5@AC;?@B(79@7A=C?>9D9C7<CB@D?C;AC=@A?;?@=D@?C><?@@?@E9AAB?BE@?@>?4@D<;??B@<:?B<?;<5613	BD:Z:KKKLNMPNLLJLMNNKAKLLMMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKJMKBLNOOMLJJ	MD:Z:83C5	RG:Z:0.1	BI:Z:OPOPMNQQPQOQRQRPIPQPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQOOQOPQOPPNLONOQPPPJOOHOQQRPPNN	NM:i:1	AS:i:84	XS:i:0
HWI-ST115_0148:1:24:10729:139872#0	16	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	:=>;@CCB?BE?7@BCA?EBCCEBCD@CCCDAC@ACA@A?A?@BFA?CBEA@D?@E?@?B?BE@BEA@8AE@>@?BA>>ACC??B@@=>AC@>AA;5843	BD:Z:LMNNLBMMMNNNLKBKKOIKBIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKINKKOLLOPIMJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQPQMGOQQRQQPPIPPRNOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMONOPOPOINNGMMPPPPJOPOQOOQQMQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:41:8779:7311#0	1040	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	:=?;>CCB?BE?8@CBA?D?8CE@CDACCCE@D?@D?@AAA?ABFA?D@E?@D?@CB@?B?BD@AD@@7AD?>@?BA>??BB@?CAA>>AC?>A?94823	BD:Z:LMNNLBMMMNNNLKBKKOIKBIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKINKKOLLOPIMJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQPQMGOQQRQQPPIPPRNOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMONOPOPOINNGMMPPPPJOPOQOOQQMQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:42:5182:53868#0	1040	chr3	58125544	60	100M	*	0	0	TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG	;=?;@CBB=BE@8ACDA?F@ABE@BB@ACBE@C@@C?@B>@>A@DA?D@E?@C?@D@@AB>BDABD@@7@D??@?BA>>?CC??B@>>>@B@>A?95844	BD:Z:LMNNLBMMMNNNLKBKKOIKBIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKINKKOLLOPIMJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQPQMGOQQRQQPPIPPRNOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMONOPOPOINNGMMPPPPJOPOQOOQQMQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:1:2492:135734#0	0	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGCGACATCTGATGACGTGCCAATGACAAACAGTCCATTAAAGGAGGC	3446??@?=@=<6@=@<A?33=<=;==@B8?A>,A?<=:@?=>BAA??A%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJNNQMDMMLNNKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMKMLHLMJMMLMMLKLHMMLIKMMLIJBKIONNMMMLKKDLOLLNN	MD:Z:54T5A12T2C0C10C2C4T3RG:Z:0.1	BI:Z:NNQQROIPPPQQNQNHONOMMGNMNNMNGGOQPRPQOPQONOQQQNQQQMOPPOONQPMQQOPQPQQPOQMQPQNPQQPMNIPNRRRPRRPQOHOMPPOO	NM:i:8	AS:i:63	XS:i:19
HWI-ST115_0148:1:5:15089:25076#0	1024	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	4533?>@A>B@;8@CC?C?@BC?@B?@BCB?@@=D@=@ACA?EB@@>@@?>A@>A@AAEB?BBAB?A=7@B=?C=A@??DCC@B?A@A?CC@B:@<:;:9	BD:Z:JJNNQMDMMLNNKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLIMJBKIONNMJLKLMLLOMIMN	MD:Z:100	RG:Z:0.1	BI:Z:NNQQROIPPPQQNQNHONOMMGNMNNMNGGOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNIPNRRRPLQOPQNOMPMOO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:61:3907:69136#0	1024	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	4633=>AA=B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@??AA>A@AAEB?C@ABAA@7AA?AC?BBBADDCAA@A@?ACC@DC?<=:9/	BD:Z:JJNNQMDMMLNNKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLIMJBKIONNMJLKLMLLOMIMN	MD:Z:100	RG:Z:0.1	BI:Z:NNQQROIPPPQQNQNHONOMMGNMNNMNGGOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNIPNRRRPLQOPQNOMPMOO	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:45:12621:117199#0	1024	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	3533=>@A>A>?8?BA=A?@A???B??BBB?A??D@>?@@>5A@?A?@?A?A=?6<0>?@:<7><@A;7@<;>B>?:8>A:C=%%%%%%%%%%%%%%%%%	BD:Z:IINNQLDLMMNNKLI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKMLIMLIMLLNMLNMMKLINMLLLMLJLIAKINNMMKMJLMKLNLIMM	MD:Z:100	RG:Z:0	BI:Z:MMPPQPIPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPQPQPKQOHOMQRQPKQPQQNOMOMOO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:22:9598:56018#0	16	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	>@:;BCB?CE@8@CCA@EACBEACEACCCEBD@@DAAAAA?@BEA?DADAAC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@>AA<98434	BD:Z:MNNKBLNMNONLKBKKOIKBJHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMKKNLKNPLMNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPQOFNPQRQQPPIPPRNPHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPNNQOOPINNGMMOPPPJOPOQONQPOQPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:47:10637:156404#0	1040	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	<?,<BAB?@D=6?@BAADACCD@BD@CCCDBD@@DA@A?B>@BEA>CADA@C?@D@AAB?BEABD@A5@D?>@?BA>>?CC@?D?A>>AB@>AB<98433	BD:Z:MNNKBLNMNONLKBKKOIKBJHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMKKNLKNPLMNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPQOFNPQRQQPPIPPRNPHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPNNQOOPINNGMMOPPPJOPOQONQPOQPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:64:7317:137760#0	1040	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	<@9<AC??BE?4@CCA@E@CCD@>C?BBBD?B?@C?@A@@>A?E@?C@D?@A?8D@@@B?BE@BD@A7@C?>@?B@>?ACC@?B???>>A?>A>;::233	BD:Z:MNNKBLNMNONLKBKKOIKBJHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMKKNLKNPLMNJJ	MD:Z:100	RG:Z:0.1	BI:Z:QPQOFNPQRQQPPIPPRNPHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPNNQOOPINNGMMOPPPJOPOQONQPOQPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:22:7690:52349#0	1040	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	=?::@AB=@B?7ABBA@D@BBD@BE@CBBD@B?@B@?@@A>?BD@?C@D@AC@@D@@?B>BDAADA@7@D?>@>B@>>?BB??C@@>>@B@>AA<98434	BD:Z:MMNKBLNNONNLLAJKNIKAIIKIIKAAIKJMNMLLMKLKMNLILLLGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJJMJJNLJOOLLMII	MD:Z:100	RG:Z:0	BI:Z:POPOENPPRQPOOHOPQMOHOMOOLOGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONRQNPOMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:44:17314:24318#0	1040	chr3	58125545	60	100M	*	0	0	ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC	;<9;@A=>BD?7?BB@@D@BBD@BE@CCBEBC?@B>@A@A>ABD@>B@D@@C@@D@@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@>AA<98434	BD:Z:MMNKBLNNONNLLAJKNIKAIIKIIKAAIKJMNMLLMKLKMNLILLLGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJJMJJNLJOOLLMII	MD:Z:100	RG:Z:0	BI:Z:POPOENPPRQPOOHOPQMOHOMOOLOGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONRQNPOMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:21:14936:171679#0	0	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	4338=>@=A??7@BBAC?@BB?AB?@BBB?@@?D@>@ACA@DB?@>@@?>AB>9?AAEB?B?AB@A@7@@@AB?BBAAECD<E>A@B?DC@D@?>@<9:<	BD:Z:JJNNMENMMNNLLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILJBKIONNMJLJLMKMONIMNN	MD:Z:100	RG:Z:0.1	BI:Z:NNQPPIQPPQQNQNHOOOMMGNMMOMMHGNQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHPNRRRPLQOPQOPNNMOOO	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:4:21260:196166#0	1024	chr3	58125546	60	100M	*	0	0	TGCAAATATGTGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCACACAGTCCCTTCAAGGTGGCT	3239>@@<A?@?@CCAD?ACC?@C?@CCC?AA?CA>@@DA?DB@@?A@?>A@>=A@?DB?C@AB@@@5@@??C=B@?BE@EBE?A8?>3C?B??%%%%%%	BD:Z:JJNNMENMMNJIIJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIIIIONNMJLJLMKMONIMNN	MD:Z:10C68A20	RG:Z:0.1	BI:Z:NNQPPIQPPQNMNNHOOOMMGNMMOMMHGNQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQMMNNRRRPLQOPQOPNNMOOO	NM:i:2	AS:i:90	XS:i:20
HWI-ST142_0240:1:7:9321:123796#0	1024	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	4338;>@=A?>7@BB@C?@BB@@A>?BBB?@@?D@>@ACA@DB?@>@@?>A@>A?@@DB?B?AB?A@7@?@AB?BB@@BBB@D?@A@ACB@D@@=>:97<	BD:Z:IINMMDNMMONKLJ@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLLIMMHMLLNMLNMMKLINMLLLMLJLIAKINNMLKMJKMKLOMIMMM	MD:Z:100	RG:Z:0	BI:Z:MMPOPIROPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPQPPKQOHOMQRQPKQOQROOOMMOON	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:1:13411:166721#0	16	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	>::>AB?BF?68CCB?E@CCD@BD@CCBD@C?<C??B???ABDA?D@D?AC?3E@@@B?BD@BDAA5@D8?@>B@>>?BB@?C@@>>AC@?AB?<?4344	BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:4:12668:186019#0	1040	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	@:<?AB?BE@7ACCAAEACCEACEACCCDBC?ADAAAAA>ABEA?CAE@BC@@D@@?B?BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?AB><=4344	BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:5:14308:33877#0	1040	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	<::?AA?BE@7ACCAAEACCEACEACCCE@C?@C?@A?A>@BDA?DAE@BC@@DA@?B>BDABDA@7@D@>@>B@>>?CB@?B??>>@B??BC><=4344	BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:5:7280:194860#0	1040	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	@:<?AB>BE@6@CDAAEACCD@CD@CCCD@C?@A?@A@@>@BE@?A@E?@C@@DAA?B>BD@BC@A7@E?>@?B@>>?CB@?B@@>>@B@?AB><?4244	BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ	MD:Z:100	RG:Z:0.1	BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:27:20013:7395#0	1040	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	?::=@A?@D?7@CC@@D@BBD@BDABBBD@D??D@@@A@>?BD@>C@D@AB@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?AB><=4344	BD:Z:MNKAKNNOONLLBJKNIKAIIKIIKAAIKJMNMLLMKLKMNMHLLLHKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJIMJJMLJMOKPMOII	MD:Z:100	RG:Z:0	BI:Z:OPOGLPOQQQOOHOPQMOHOMOOMNHGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPQNROQMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:63:14584:29777#0	1040	chr3	58125546	60	100M	*	0	0	TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT	=::=@A>BD?7@CB@@D@BBD@BDABCBEBD??D@@AA@>?BD@?B@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C?@>>@B@?AB><=4334	BD:Z:MNKAKNNOONLLBJKNIKAIIKIIKAAIKJMNMLLMKLKMNMHLLLHKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJIMJJMLJMOKPMOII	MD:Z:100	RG:Z:0	BI:Z:OPOGLPOQQQOOHOPQMOHOMOOMNHGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPQNROQMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:27:2568:76725#0	0	chr3	58125547	60	100M	*	0	0	GCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTG	4386;><A>?7@BBAC?@AB?@B?@BBB?A@?D>:@@C@@DB?@>@@@2@>>8?@@B?=@@AA=A@5@@?>D;B=>>BBB>D<?>=>%%%%%%%%%%%%%	BD:Z:IIMIENOMOOKLJAIILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKIMMILLLNMLNMMKLINMLLLMLJLIAKINNMLJMJKLKLONJMMMM	MD:Z:100	RG:Z:0	BI:Z:MMONIRQPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPQOKQOHOMQRQPKQOPRPPOOKOONP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:27:6383:156593#0	16	chr3	58125547	60	100M	*	0	0	GCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTG	%%%=/>@A<4?CCA@B<>-D=.A?B@CC=C?@C?@A=@=@BD@?C@B>??;;A5>@@<>>??C?A//;3';:>?>><CB>@B1@<>?A??A>:=@:6454	BD:Z:NKAKMMOOOMKBKKOIKBJIKIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJNKJMNKPQOOJJ	MD:Z:100	RG:Z:0.1	BI:Z:QOHONOQQQPPIPPRNPIOMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOPOPOIONGMMOOOPJOPOQONPONRRRQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:28:2897:141354#0	1040	chr3	58125547	60	100M	*	0	0	TCAAATATGCGTTTCTGCTTATTGTTTTGAGCAGGGCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTACCTTCAAGGTGGCTG	%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%C:@A@;AC5.:.=327')16913(@BC@93)229=>>=:856:33752	BD:Z:NKAKMMOOOMKBKKONOKJLLIHJAAIKJMMMKIMNKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLLLMJJNKJMNKPQOOJJ	MD:Z:0G16T2G14T48C15	RG:Z:0.1	BI:Z:QOHONOQQQPPIPPRQSQOPPNMOHHNPNOQPPKPRPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOPOPOIONGMMOOOQPOPOQONPONRRRQNN	NM:i:5	AS:i:79	XS:i:19
HWI-ST142_0240:1:67:5306:137734#0	1040	chr3	58125547	60	100M	*	0	0	GCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTG	::==@>BD?7@BC@@D@BBD@BD@CBCEBD@?CA@A?A>?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?BB?>@93484	BD:Z:NKAJMNOOOLLBKKNIKAIIKIIKAAIKJMNMLLMKLKMNMIKLLHLKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJILJJMKJMMKOQONII	MD:Z:100	RG:Z:0	BI:Z:POGNNOPPQPOHOPQMOHOMOOMOGHNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPONRQQPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:8:1653:130740#0	1024	chr3	58125548	37	27M73S	*	0	0	CAAATATGCGTTTCTGTTTGTTGTTTTTGAAAGGTGTCCCTAAAATAACGAGGACACAAAACAGCGAGCCCATACCAATGATAGCTAACACAGCGACTGC	%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%	BD:Z:JJJBOONOOLMK@JJMIJ@JIJJIJ@@@JMJAJMLHIMLIKKIAAKKIJKMKMKLHHHIAAJHNNKMKNMILLKLLLIKNNMLNONLJKIJJPQMOMLNN	MD:Z:27	RG:Z:0.1	BI:Z:NNNHRQQQQNQNHOOPNMGNMMNMNGGHNPOHPOPMNQOKPPNHHPPNOOQPOPPMMMNHHOMQRNQPRPKQQPPOQNPQQQQRSPQOPNNNRRMOPPPQ	NM:i:0	AS:i:27	XS:i:20
HWI-ST115_0148:1:8:2670:193217#0	0	chr3	58125548	60	100M	*	0	0	CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT	4866;:@>>7@BBAC?@BB?@B?@BBB?@??E@>AACA@EB?@>@A?>A@>A@A@@?=BBAB@@@7AB??B=B>?BDCC@EA@?A?@C@DB??@@=;=;<	BD:Z:JJJBOONOOLMK@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAKIONNMJLJKLKLOOJNNNNJ	MD:Z:100	RG:Z:0.1	BI:Z:NNNHRQQQQNQNHOOPNMGNMMNMNGGOPORPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMRRRPLQOPQOQPPLMOOPN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:22:15967:185854#0	1024	chr3	58125548	60	100M	*	0	0	CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT	4756;:@>>7@BBAC?@BB??B??BBB?A@?D@>@ACA@DB?@>@@?>A@>=?A@DB?B?@BA@@7AA?>B?B@>ADBB@D?AA?@BC@@B?>@?<:;97	BD:Z:IIIAOOOOOLLJAJILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKHMMIMKLNMLNMMKLINMLLLMLJLIAKINNMLJLJKLJLONKNMMMI	MD:Z:100	RG:Z:0	BI:Z:MMNGRQRPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPPPJQOHOMQRQPKQOPQPQPOMMONPM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:26:7294:74712#0	16	chr3	58125548	60	100M	*	0	0	CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT	;==>>BD@9ACCAAEACBEACEACCCDBD@@CAAAAA?@CEA?DAEABCA@DA@AB>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?BC??B<84844	BD:Z:KAKLMNPOMLBKKOIKBJIKJHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMKJLNJOQROIJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHOPNPPQPPIPPRNPIONONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQOOPINOGMMOOOOJOPOQONPOMRQTQMNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:8:17018:130894#0	1040	chr3	58125548	60	100M	*	0	0	CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT	;==>=BE>6ACCABEBC@F@CEACCCDBC@@C?AB@A>@BE@?C@D=?=>8C<:@0?BDABD@@7AE?>?=BA>>?CB@?C@@=>?C@?BC??B<85844	BD:Z:KAKLMNPOMLBKKOIKBJIKJHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMKJLNJOQROIJJ	MD:Z:100	RG:Z:0.1	BI:Z:OHOPNPPQPPIPPRNPIONONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQOOPINOGMMOOOOJOPOQONPOMRQTQMNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:27:17632:7595#0	16	chr3	58125549	60	100M	*	0	0	AAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTC	:=;;?E@7ACCA?D=@CD=>E@CCCEBB@AC?@A>A>@BEA?C@EA@D@@D@AAB>BDABDAA7@D?>?>BA>>?BCA?C@A>>@B@?BC@?C>;98444	BD:Z:AKLLNOPMLCKKOIKBJIKJIJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJJLMJNPRRIMJJ	MD:Z:100	RG:Z:0.1	BI:Z:HOPPOOPPPIPPRNPIONPNMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPOOJNNHMMOOOOIOPOQONPOMQQSSMPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:44:19454:138733#0	1040	chr3	58125549	60	100M	*	0	0	AAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTC	;=<7@D?7@CCAAEACCEACEACCCE@C@ACAAA@A>ABEA?BAEABC>@EAA?B>BEABEAA7@D?>@?BA>>?BB@?C@@>>@B@?CC@?C>;98444	BD:Z:AKLLNOPMLCKKOIKBJIKJIJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJJLMJNPRRIMJJ	MD:Z:100	RG:Z:0.1	BI:Z:HOPPOOPPPIPPRNPIONPNMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPOOJNNHMMOOOOIOPOQONPOMQQSSMPNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:8:1814:174888#0	1040	chr3	58125549	60	7S93M	*	0	0	CCGATCTAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGT	-39>?=?CDB?2E?8@CCA>D=6CB@@C@?CCDBD@AB?BB>A>@BE@>C@C?@B?>D@>AA?=DABAA@8@>?>??BA>>ABC@?C?@>>?A><;8433	BD:Z:MLNMLOLBLMLMNNLKBKKOIJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLLJNLLPNMLMJJ	MD:Z:93	RG:Z:0.1	BI:Z:OOQPMPPIPQQRQQPPIPPQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQNLPNNQOOOINNGNNPPPPJOPORPPPONN	NM:i:0	AS:i:93	XS:i:0
HWI-ST115_0148:1:1:20930:85007#0	0	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	4662;;>6?BBAC?@BC?@B?@BBB?A@?E@>@ACA@DB?@>@@?>A@>@?@@EB?BAAB@B@6@A>?C?B@?>CCC@E@A@??CC@DC@?;=?=A>;9=	BD:Z:JJLLOQPMNKAKJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHONNMJLJKLJKONJOOONJNL	MD:Z:100	RG:Z:0.1	BI:Z:NNPPRRRNQNHOOPNNHNMMNMMGHNPPQOQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQRPLQOPQOQPQNONMPNQP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:26:13065:57592#0	1024	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	4662;;=6?BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>A@>@@AAEC?C@AC@A@8AA@AD?C@AAECCAEAABAADCBBC@>>B>?A<;;6	BD:Z:JJLLOQPMNKAKJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHONNMJLJKLJKONJOOONJNL	MD:Z:100	RG:Z:0.1	BI:Z:NNPPRRRNQNHOOPNNHNMMNMMGHNPPQOQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQRPLQOPQOQPQNONMPNQP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:44:16873:9543#0	1024	chr3	58125550	60	89M11S	*	0	0	TATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAAGATCGGAAGA	3354<;=6?CCAB@ACB?ABA?CCB@??=EA>@@CA@DA?@>@?@=@@=;@A>BA?BA@B@A?5>A?>>?B???BCC@D?AB??CC@DCCABB>7=><:;	BD:Z:JJLLOQPMNKAKJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHONNMJLJKLJBLLNPMNLKKK	MD:Z:0A88	RG:Z:0.1	BI:Z:NNPPRRRNQNHOOPNNHNMMNMMGHNPPQOQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQRPLQOPQOIQPQQNMPOPO	NM:i:1	AS:i:88	XS:i:0
HWI-ST142_0240:1:66:6615:16862#0	1024	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	4662;;=6?BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>@@>A?@@DB>B?@B@@A7@??@C?CBAADCB@B@@A@@CBAEB@>@@>>A;98=	BD:Z:IIKLPQQLMKAJJMHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKHLLIMLLMMLNMMKLINMLLLMLJLIAKINNMLJLIJLJKNNKOONMIMK	MD:Z:100	RG:Z:0	BI:Z:MMPORRRMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPPOJQNHOMQRQPKQOPQOPQQNOOLPMPP	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:8:13721:15726#0	1024	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	4662;;=6?BBAC?@BB?@B?@BBB?@@?D@>@AC@@DB?@?@@?>A@>=?A@DB>B?@B@A?7@@>@B?B@AADCB@C@=A@@BB@E===@@>>?;9::	BD:Z:IIKLPQQLMKAJJMHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKHLLIMLLMMLNMMKLINMLLLMLJLIAKINNMLJLIJLJKNNKOONMIMK	MD:Z:100	RG:Z:0	BI:Z:MMPORRRMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPPOJQNHOMQRQPKQOPQOPQQNOOLPMPP	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:43:4061:135226#0	16	chr3	58125550	60	100M	*	0	0	AATATGCGTCTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	;<9>;?6?A?AADACCBACDACCCE@C?@CA@A?B?ACC@?CAEA@D?@EA@AB>BD@BDAA7@D?>?>B@>>?BB@@C@@>>@B@?BC@AC?=>=4434	BD:Z:KLLMOONNLMKOIKBJIKJIKAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJILMINOQRLMNJJ	MD:Z:9T90	RG:Z:0.1	BI:Z:OPPQNOOQPQPRNPIONPOMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPPOIONGNMOOOOINPOQONPOMQPSROPQNN	NM:i:1	AS:i:95	XS:i:0
HWI-ST115_0148:1:64:5894:89026#0	1040	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	=;8>B>7@CCA?EACCEACEACCCEBD?@CAAAAA>ABEA?CAE?BDA@E@@AB>BEABEA@7@E?>@>B@>>?BB@?C@@>>@BA?BC@@C?=<=4434	BD:Z:KLLMOONLCLKOIKBJIKJIKAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJILMINOQRLMNJJ	MD:Z:100	RG:Z:0.1	BI:Z:OPPQNOOPIPPRNPIONPOMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPPOIONGNMOOOOINPOQONPOMQPSROPQNN	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:3:12949:146598#0	1040	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	9<8<B>7?BA@@E@ABD@C7@BBBD@B?@B>?BAB=@BC?>C@D@AC=@D?@?B>AD@BDA@7@C?>??B@>>?BB@?A@@=>@B@?BC@@C?=<=5434	BD:Z:JLLMNOMMCKLOJKAIIKIIKAAIKJMNMLLMKLKMNMILLLGLLJLKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJILIILKILLIMNRQMMMII	MD:Z:100	RG:Z:0	BI:Z:NPOPMONOIPPQMOHOMOOMOHHOPONPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPOLPOSRNPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:63:20198:59364#0	1040	chr3	58125550	60	13S87M	*	0	0	GCTCTTCCGATCTAATATGCGTTTCTGCTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC	58;:?@=6<6A@?BB?@D>2@CBA@D?@@D?AC?BBC=@C?AC@@A@B>?BD@?B@D@@B@@D?@?B>BD@BD@?7?C==@?>@=>?BB@>B?>:74634	BD:Z:OLKJKMNMOMLNKJLLMMMKKAJKNMOJIIKIIKAAIKJMNMKLMKLJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILKAJINMNNMQJJII	MD:Z:14T72	RG:Z:0	BI:Z:QOOOLPMORRPQQOQPQPPOOHOPPPQONLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPRLQONMM	NM:i:1	AS:i:82	XS:i:19
HWI-ST142_0240:1:6:5523:114409#0	1040	chr3	58125550	60	100M	*	0	0	AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA	==9=B>7@BBA@D@BAD@B>@BBBEAD@@BA@AA@>?AD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?BC@@C?=<=4434	BD:Z:JLLMNOMMCKLOJKAIIKIIKAAIKJMNMLLMKLKMNMILLLGLLJLKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJILIILKILLIMNRQMMMII	MD:Z:100	RG:Z:0	BI:Z:NPOPMONOIPPQMOHOMOOMOHHOPONPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPOLPOSRNPPMM	NM:i:0	AS:i:100	XS:i:0
HWI-ST115_0148:1:28:10415:169781#0	0	chr3	58125551	60	100M	*	0	0	ATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCAC	46268=6?ABAC?@BB?@C@@CCC?A??D@>@ACA@DB?@>@@@?A<?@@>BEB?C@ACAAA6A@@AD?BB?ADCCAC?A@BADCADCA>A@>?5>=:?8	BD:Z:JJLLQQNNLAKKMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHNNNMJLJKLJKNNJNPOOJNLI	MD:Z:100	RG:Z:0.1	BI:Z:NNPPSROQNHOOPNNHOMMNMMGGOPOROPOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQQPLQOPQOQPQNPONNNQPM	NM:i:0	AS:i:100	XS:i:0
HWI-ST142_0240:1:26:12059:67588#0	1024	chr3	58125551	60	79M21S	*	0	0	......

Choosing SNPs

How to choose SNPs as the input for alleleCount? More specifically;

How many SNPs are sufficient throughout the genome?
How should the SNPs be distributed along the genome? i.e. in terms of window size, genomic region, ... ?

.exe file

Hello,
I have been trying to install alleleCount in order to run it in ASCAT. For that purpose I need an .exe file. Unless I'm missing something, the setup.sh provided installs the dependencies.
Could you provide some instructions on how to get the .exe?

Thanks and best wishes
Georgios

Lines 74 to 75 in 41c4b5a

    
            printf ("                                 and filter out any proper-pair flagged reads not in F/R orientation."); 
        
           printf (" -F  --filtered-flag [int]       Flag value of reads to exclude in allele counting default: [%i].\n",exc_flag);

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	{"required-flag", no_argument, 0, 'f'},
	{"filtered-flag", no_argument, 0, 'F'},

	printf (" and filter out any proper-pair flagged reads not in F/R orientation.");
	printf (" -F --filtered-flag [int] Flag value of reads to exclude in allele counting default: [%i].\n",exc_flag);