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abolia avatar brendanofallon avatar durtschi avatar jelber2 avatar luobiny avatar

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jelber2

jovian's Issues

Possible for long reads or tool to shred long alignments to non-overlapping records?

Hi,

I have some HG002 long reads that I have error-corrected to almost short-read quality - https://gist.github.com/jelber2/02fdca09ed3b12b650b512ba200f3b67

I was wondering if there might be a tool to shred my alignments say from 20000-bp (just for example) to 150-bp or whatever the maximum read length allowed by jenever is. I guess I could try to make either a Rust (or Julia) tool to do this, but I was asking just in case someone has something already. I know of some tools for unaligned reads, but not for already-aligned reads.

Best,
Jean

Training a long-read model as if the alignments were short reads

Dear Brendan,

I know that you were considering something else in terms of making a long-read model, but I wanted to try and make a model and classifier based on these CRAM files, after shredding to 150-bp alignments pieces

, but I am a little confused as

jovian/README.md

Lines 99 to 101 in 0fe88c5

The BED files must be four-column BED files where the fourth column is the region label.
The label scheme is completely flexible and up to the user, but in general should match the values
in 'vals_per_class' from the configuration file.

seems to suggest that the fourth column of the bed file for the config.yaml (from GIAB for example) would be subdivided into the value classes one sees below

jovian/README.md

Lines 83 to 87 in 0fe88c5

vals_per_class:
'snv': 5000 # keys here should match labels in fourth column of BED files provided below
'deletion': 500
'insertion': 500
'mnv': 1000

Am I correct that such a bed file would be subdivided based on the vals_per_class? I would really be interested in seeing how well a long-read model (even a "shredded" one) for jenever performs on Nanopore SUP_Herro data that I had sent you links for.

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