Comments (2)
Hi, Eric. Is the data from 2018 too or just the machine? I'm wondering if the chemistry is modern,
I would first recommend trying :
goldrush run reads=all-ont-reads G=3e9 t=60 m=10000
if the average read length is 12,000.
GoldRush works better with longer reads and does not need all available reads.
If the data is old, you may have to change the P
parameter to 10.
I should note that this is quite exploratory as we have not tried assembling repeat-rich datasets with low read length. High read lengths aids GoldRush in distinguishing repeats to an extent and scaffolding.
Regards,
Johnathan
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This issue has been automatically marked as stale because it has not had recent activity. It will be closed if no further activity occurs. Thank you for your interest in GoldRush!
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