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heoa's Introduction

HEOA: human Early Organogenesis Atlas

1. This repository contains the R code used in the following paper on human emrbyo:

A single cell transcriptome atlas of human early embryogenesis

2. The introduction for file structure

  1. src/: source codes for each figure (corresponding to *.html) and all other analysis (e.g., identification of cell types and identification of systemically changing genes). All custom functions were stored in 'function.r' and 'functions.r'.
  2. data/: necessary input data for scripts: gene annotation, cell annotation, color scheme, and contours of limb domains.
  3. list/: gene list from published data: batch effect genes, cell cycle genes, and hemoglobin genes.
  4. plot/: figures generated in the analysis that are not included in R markdown files.
  5. remap_Tan_frog: the script to map RNA-seq data of frog embryos (Tan et al. 2013) on latest frog genome.

3. An online depository for cell types and gene expression is avaiable at https://heoa.shinyapps.io/base/.

4. The raw and processed data can be downloaded from the NCBI Gene Expression Omnibus (GSE157329).

heoa's People

Contributors

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Stargazers

Zhiyuan avatar Koh avatar  avatar Biao Zhang avatar onurcanbektas avatar  avatar  avatar Ryan J. Andrews avatar  avatar slp avatar Xue Huiwen avatar  avatar Tianming Wu avatar

Watchers

Ryan J. Andrews avatar

heoa's Issues

the rdata for figure1a

Dear professor,

We want to observe the craniofacial cells in human embryos, but the result of umap in shiny is not repeated in our own code,
is it convenient to provide the Normalized SeuratObject in craniofacial?

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