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Sinejune avatar Sinejune commented on June 8, 2024 1

I had the same problem, you can try upgrading matrix to 1.6.1.1 and seuratobject to version 5.0, hope this helps!

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zhanghao-njmu avatar zhanghao-njmu commented on June 8, 2024

Strange. In Seurat v4 or SeuratObject v4, there should not be a function called LayerData. This function is only exported in version 5. Therefore, please double-check if the version of Seurat in your environment is indeed v4.

https://github.com/satijalab/seurat/blob/727d26176d87c96c36ccd79d51219e66c9f0da37/NAMESPACE#L517C26-L517C35
https://github.com/mojaveazure/seurat-object/blob/89fe70b832602dd5dc4f846ccd086223a3129fa5/NAMESPACE#L36

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kli28 avatar kli28 commented on June 8, 2024

Same with this problem. When I installed SCP following the instructions, it installed seurat v5.0 by default. And then I just changed it to Seurat 4.4.0. Then I met the problem. But I think upgrading seuratobject to version 5.0 is not a good idea? Because most of the tools in SCP may be supported by Seurat 4 ? So is it possible to be forced to install Seurat 4?

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kli28 avatar kli28 commented on June 8, 2024

@Streisenberg Hi, Have you solved this problem in other ways instead of changing seuratobject to version 5.0?

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Jaimelan avatar Jaimelan commented on June 8, 2024

I also have this problem with seurat 4.4.0

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xiaodiYan avatar xiaodiYan commented on June 8, 2024

I also have this problem with seurat 4.4.0

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