Comments (1)
Thanks for checking out vsnp3 and sending issues seen.
I've had inconsistent results with vcftools and bcftools. I typically see bcftools installed via the freebayes requirement so have left it out from explicit requirement list. Same with vcflib for vcftools. I've fought with conda installing bcftools as a Python 2 tool when asking for Python 3 when specifying the install explicitly. I've had best results leaving them out of the explicit requirements and letting them be installed as requirements of freebayes and vcflib. Same with the libcrypto (and other libraries). Other than having comments like this here to help other users, I am convinced that because everyone's environment is slightly different conda may require troubleshooting to either "fix" a user's environment or to fix something being overlooked by conda. That being said I should look at replacing these tools since they're often problematic. I did this for pysam/samtools. These tools would often (but not always) cause conflicting libraries, so pysam was removed from vsnp3. I will be working soon to provide vsnp3 as a container. Hopefully this will ease installation, or at least provide another option.
Nanopore is beta at best. Especially since the technology is steadily changing. Can you share the FASTQ file you're using? If so I would like to troubleshoot.
Sourmash runs quick and I like seeing the "best reference" even when specifying. I should change my wording so there isn't confusion. It should still be using the reference you specified. I'm going to update the wording.
I would like to improve Nanopore support. This has been a first test at seeing how it may work, but the datasets tried so far have been few. This input is good to get.
from vsnp3.
Related Issues (4)
- vSNP3 HOT 6
- Best_ref error
- vcflib unable find libraries HOT 1
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from vsnp3.