Comments (1)
Hi @ofiryaish,
At the moment Cas-OFFinder does not support multiple gaps.
Regarding this:
Moreover, It seems that the tool is providing cRNA with 24 nuclides (when the original size is 23), meaning that it seems like it > contains insertion in the guide RNA. How is it possible? The insertion or deletion should be only in the DNA.
It is not regarding indels but bulges:
from cas-offinder.
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from cas-offinder.