Comments (7)
The same error as @kaizen89
R version 3.6.2 (2019-12-12)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 18363)
other attached packages:
[1] RColorBrewer_1.1-2 liger_0.5.0.9000 patchwork_1.0.0 Matrix_1.2-17 cowplot_1.0.0 SeuratWrappers_0.1.0
[7] Seurat_3.1.2
Thanks
from seurat-wrappers.
Same error using the pbmcsca data set from SeuratData.
I just ran the following:
data("pbmcsca")
pbmcsca <- NormalizeData(pbmcsca)
pbmcsca <- FindVariableFeatures(pbmcsca)
pbmcsca <- ScaleData(pbmcsca, split.by = "Method", do.center = FALSE)
pbmcsca <- RunOptimizeALS(pbmcsca, k = 20, lambda = 5, split.by = "Method")
pbmcsca <- RunQuantileAlignSNF(pbmcsca, split.by = "Method")
R version 3.6.1 (2019-07-05)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Antergos Linux
other attached packages:
[1] liger_0.5.0.9000 patchwork_1.0.0 Matrix_1.2-17 cowplot_1.0.0 SeuratWrappers_0.1.0
[6] pbmcsca.SeuratData_3.0.0 pbmc3k.SeuratData_3.0.0 hcabm40k.SeuratData_3.0.0 SeuratData_0.2.1 Seurat_3.1.4
from seurat-wrappers.
got same error.
from seurat-wrappers.
same error
sessionInfo()
R version 3.6.1 (2019-07-05)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS Mojave 10.14.6
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] SeuratWrappers_0.1.0 pbmcsca.SeuratData_3.0.0 ifnb.SeuratData_3.0.0 SeuratData_0.2.1 Seurat_3.1.2 liger_0.5.0.9000 patchwork_1.0.0
[8] Matrix_1.2-18 cowplot_1.0.0 devtools_2.2.1 usethis_1.5.1
from seurat-wrappers.
I just did not used the Wrapper in the end. Probably Liger updated the function and wrapper didn't udate it.
I used this and it worked. Then you can just transfer the projection to seurat object again
data.liger <- createLiger(sapply(ob.list, function(data) data[['RNA']]@counts[, colnames(data)]), remove.missing = F)
data.liger <- liger::normalize(data.liger)
[email protected] <- var.genes
data.liger <- scaleNotCenter(data.liger)
data.liger <- optimizeALS(data.liger, k = k, lamda = lamda)
data.liger <- quantileAlignSNF(data.liger, resolution = 1.2)
data.liger <- runUMAP(data.liger, n_neighbors = n_neighbors, min_dist = 0.3)
seurat_obj = ligerToSeurat(data.liger, use.liger.genes = T)
from seurat-wrappers.
Hi, I answered this here
They have updated liger. you need to use liger 0.4.2 to have wrapper functionality, until the wrappers are updated.
from seurat-wrappers.
This was addressed in #41
from seurat-wrappers.
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