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ptbrown1729 avatar ptbrown1729 commented on August 15, 2024

@dpshepherd @fdjutant per our discussion at subgroup meeting today:

Current plan for the Galvo branch: all of the code except for the localization code has already been moved to master. Create a new branch from master and add the localization code (at this point Galvo will be defunct). We can use this as a development branch until the localization code is fully vetted, then it will be merged back into master.

We will also make a single file in master for data/metadata loading. I can take a first crack at this, but will need input from everyone to ensure it works for all experiments. This is likely to break some workflows at the beginning.

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ptbrown1729 avatar ptbrown1729 commented on August 15, 2024

@dpshepherd @fdjutant I think we can delete the galvo branch now right? No one is using it anymore and the relevant code should be on master or localization

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fdjutant avatar fdjutant commented on August 15, 2024

@ptbrown1729. I agree. I just use the master recently for the recon stuff

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dpshepherd avatar dpshepherd commented on August 15, 2024

This looks good and thanks for deleting. The next consolidation effort should be to get an nearly automated pipeline for iterative RNA-FISH localization with coordinate transforms between tiles, channels, and rounds determined using either Python or BigStitcher. I still think BigStitcher does a better job calculating the coordinate transforms, but we can look into other existing Python solutions so that we don't have to make command line calls to BigStitcher. Because BigStitcher can run in FIJI's headless mode, that isn't that hard to include in Python.

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