Comments (6)
i opened an issue with them now.
ncbi/sra-tools#865 (comment)
from fetchngs.
I got the same bug, (and my email , full of dots, is in my path...). If fixed this on my side for paired-ends reads with the following code for fetchngs/modules/nf-core/sratools/fasterqdump/main.nf
mkdir -p TEST.OUT TEST_1.OUT TEST_2.OUT
fasterq-dump \\
$args \\
--threads $task.cpus \\
--outfile TEST.OUT/$outfile \\
${key_file} \\
${sra}
mv -v "TEST_1.OUT/${outfile}" ./${outfile}.1.fastq
mv -v "TEST_2.OUT/${outfile}" ./${outfile}.2.fastq
pigz \\
$args2 \\
--no-name \\
--processes $task.cpus \\
*.fastq
(...)
from fetchngs.
This should be fixed in the next pipeline release by downgrading the version of sratools until it is fixed upstream. See #261
I will leave this issue open until then as fixing upstream is the desired resolution. In the meantime, anyone wanting to use more recent support for ngc
files can use sratools v3.0.8 via a custom config file passed to the pipeline:
process {
withName: 'SRATOOLS_FASTERQDUMP' {
container = 'quay.io/biocontainers/mulled-v2-5f89fe0cd045cb1d615630b9261a1d17943a9b6a:2f4a4c900edd6801ff0068c2b3048b4459d119eb-0'
}
}
from fetchngs.
looking at the code and the actual command.sh file (see below) I actually think this is a fasterq-dump issue because the error message seems to be printed by fasterq-dump
#!/bin/bash -euo pipefail
export NCBI_SETTINGS="$PWD/user-settings.mkfg"
fasterq-dump \
--split-files --include-technical \
--threads 6 \
--outfile SRX10737613_SRR14385311 \
\
SRR14385311
pigz \
\
--no-name \
--processes 6 \
*.fastq
cat <<-END_VERSIONS > versions.yml
"NFCORE_FETCHNGS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP":
sratools: $(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
pigz: $( pigz --version 2>&1 | sed 's/pigz //g' )
END_VERSIONS
from fetchngs.
I am able to reproduce this locally when running nf-test:
nf-test test --verbose --tag sra_default_parameters --profile test,docker
from fetchngs.
As outlined in ncbi/sra-tools#865 (comment) fasterq-dump
is unable to resolve paths that contain a .
for writing the output files.
from fetchngs.
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from fetchngs.