What's the lowercase letters about nextpolish HOT 7 CLOSED

1. The lowercase letters are low quality bases.
2. No, it means the assembly includes more deletion errors than insertion errors, so the genome size will larger than original after fixing more deletion errors.

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How about the lowercase letters in the output of NextDenovo? The original genome produced by NextDenovo also contained some lowercase letters.

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see here

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Sorry, I didn't notice. Many thanks!

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Hi，all,

Genome had ‘N’ base after nextpolish, what's the N base means? The original assembly from long reads had no N base.

Thanks a lot!

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See FAQ vi.

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Thanks !

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