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alauring avatar alauring commented on August 15, 2024

Thanks for the comment. We had some issues with this ourselves and were able to get a workaround with the folks who manage our cluster. Feel free to fork our repository and use BiocManager. Please let us know if that provides a more robust solution and perhaps send a pull request?

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grendon avatar grendon commented on August 15, 2024

I am trying my luck with modules and nextflow instead of using your approach with conda and packrat. If I get anywhere I will be happy to post my version as a branch (perhaps?)

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grendon avatar grendon commented on August 15, 2024

Would it be possible for you to post results files within the tutorial
so that we can compare our results to your, please?

This is what deepsnv is generating for us in the output.csv file:

chr pos ref var p.val freq.var sigma2.freq.var n.tst.fw cov.tst.fw n.tst.bw cov.tst.bw n.ctrl.fw cov.ctrl.fw n.ctrl.bw cov.ctrl.bw raw.p.val Id mutation
HA 784 A G 0 1 0.000697 1018 1018 416 416 0 1146 0 215 0 HA-22_S7_L001 HA_A784G
M 4 G A 0.008994 0.147545 0.000885 421 883 1 3 309 937 2 9 1.66E-07 HA-22_S7_L001 M_G4A
NP 4 G A 0.000134 0.192342 0.001148 334 725 1 3 138 518 1 1 2.47E-09 HA-22_S7_L001 NP_G4A

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alauring avatar alauring commented on August 15, 2024

That looks like a typical output file for DeepSNV. I do not know if the data are exactly like what we get in our tutorial, but suspect that everything is working properly here!

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