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leepc12 avatar leepc12 commented on August 23, 2024

It looks like the estimated fragment length is negative.
This pipeline is no longer maintained.
Please use https://github.com/ENCODE-DCC/chip-seq-pipeline2.
I think this issue is already fixed in the new pipeline.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

Please read through README on https://github.com/ENCODE-DCC/chip-seq-pipeline2.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

It should be caper run chip-seq-pipeline2/chip.wdl -i testHg19.json

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

Check line 13 column 29 of your input JSON. Check commas, brackets, ... For example, TAB is not allowed in JSON.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

Delete lock file /home/ysun/.caper/cromwell_jar/*.lock and try again. Make sure that you have only one process to run a pipeline.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

Find metadata.json instead of qc.json.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

Can you upgrade croo to 0.3.3 and try again?
pip install croo==0.3.3

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

Can you find/modify your cromwell_metadata.py like the following?

How to find it:

leepc12@kadru:/users/leepc12/code/croo$ python
Python 3.7.3 (default, Mar 27 2019, 22:11:17)
[GCC 7.3.0] :: Anaconda, Inc. on linux
Type "help", "copyright", "credits" or "license" for more information.
>>> import croo
>>> import croo.cromwell_metadata
>>> croo.cromwell_metadata.__file__
'/users/leepc12/code/croo/croo/cromwell_metadata.py'
>>>

Replace this (from line 183)

                for output_name, output_path, _ in out_files:
                    # add each output file to DAG
                    n = CMNode(
                        type='output',
                        shard_idx=shard_idx,
                        task_name=task_name,
                        output_name=output_name,
                        output_path=output_path,
                        all_outputs=None,
                        all_inputs=None)
                    self._dag.add_node(n)

with this

                if out_files:
                    for output_name, output_path, _ in out_files:
                        # add each output file to DAG
                        n = CMNode(
                            type='output',
                            shard_idx=shard_idx,
                            task_name=task_name,
                            output_name=output_name,
                            output_path=output_path,
                            all_outputs=None,
                            all_inputs=None)
                        self._dag.add_node(n)

If this works, I will make a hot fix.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

That sounds good.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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leepc12 avatar leepc12 commented on August 23, 2024

It's Conda's issue. Conda has changed its activation method from source activate to conda activate. source activate worked fine in a BASH script but conda activate doesn't.

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yingsun-ucsd avatar yingsun-ucsd commented on August 23, 2024

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